LOCUS       sequence-35529-e         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway donor vector containing HRAS G12V..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Yang X, Boehm JS, Yang X, Salehi-Ashtiani K, Hao T, Shen Y, Lubonja 
            R, Thomas SR, Alkan O, Bhimdi T, Green TM, Johannessen CM, Silver 
            SJ, Nguyen C, Murray RR, Hieronymus H, Balcha D, Fan C, Lin C, 
            Ghamsari L, Vidal M, Hahn WC, Hill DE, Root DE
  TITLE     A public genome-scale lentiviral expression library of human ORFs.
  JOURNAL   Nat Methods. 2011 Jun 26;8(8):659-61. doi: 10.1038/nmeth.1638
  PUBMED    21706014
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-06-26- 26"; volume: "8"; 
            issue: "8"; pages: "659-61"
COMMENT     Sequence Label: pDONR223-H-RAS V12
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    3..102
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(join(104..637,638..640,641..673))
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase H-Ras"
                     /label=H-Ras (G12V)
                     /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVK
                     DSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREI
                     RQHKLRKLNPPDESGPGCMSCKCVLS"
     protein_bind    complement(676..700)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aggcaataat gattttattt tgactgatag tgacctgttc gttgcaacaa attgataagc
       61 aatgctttct tataatgcca actttgtaca agaaagttgg caatcaggag agcacacact
      121 tgcagctcat gcagccgggg ccactctcat caggagggtt cagcttccgc agcttgtgct
      181 gccggatctc acgcaccaac gtgtagaagg catcctccac tccctgccgg gtcttggccg
      241 aggtctcgat gtaggggatg ccgtagcttc gggcgaggtc ctgagcctgc cgagattcca
      301 cagtgcgtgc agccaggtca cacttgttcc ccaccagcac catgggcacg tcatccgagt
      361 ccttcacccg tttgatctgc tccctgtact ggtggatgtc ctcaaaagac ttggtgttgt
      421 tgatggcaaa cacacacagg aagccctccc cggtgcgcat gtactggtcc cgcatggcgc
      481 tgtactcctc ctggccggcg gtatccagga tgtccaacag gcacgtctcc ccatcaatga
      541 ccacctgctt ccggtaggaa tcctctatag tggggtcgta ttcgtccaca aaatggttct
      601 ggatcagctg gatggtcagc gcactcttgc ccacaccgac ggcgcccacc accaccagct
      661 tatattccgt catggtgcca acttttttgt acaaagttgg cattataaaa aagcattgct
      721 catcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt att
//
