LOCUS       sequence-35530-e         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway donor vector containing HRAS G12V..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Yang X, Boehm JS, Yang X, Salehi-Ashtiani K, Hao T, Shen Y, Lubonja 
            R, Thomas SR, Alkan O, Bhimdi T, Green TM, Johannessen CM, Silver 
            SJ, Nguyen C, Murray RR, Hieronymus H, Balcha D, Fan C, Lin C, 
            Ghamsari L, Vidal M, Hahn WC, Hill DE, Root DE
  TITLE     A public genome-scale lentiviral expression library of human ORFs.
  JOURNAL   Nat Methods. 2011 Jun 26;8(8):659-61. doi: 10.1038/nmeth.1638
  PUBMED    21706014
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-06-26- 26"; volume: "8"; 
            issue: "8"; pages: "659-61"
COMMENT     Sequence Label: pDONR223-H-RAS V12
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    7..106
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(109..141,142..144,145..678)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase H-Ras"
                     /label=H-Ras (G12V)
                     /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVK
                     DSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDXFYTLVREI
                     RQHKLRKLNPPDESGPGCMSCKCVLS"
     protein_bind    complement(680..779)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(796..815)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(797..815)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(820..836)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(820..836)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tcgggcccaa taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat
       61 gagcaatgct tttttataat gccaactttg tacaaaaaag ttggcaccat gacggaatat
      121 aagctggtgg tggtgggcgc cgtcggtgtg ggcaagagtg cgctgaccat ccagctgatc
      181 cagaaccatt ttgtggacga atacgacccc actatagagg attcctaccg gaagcaggtg
      241 gtcattgatg gggagacgtg cctgttggac atcctggata ccgccggcca ggaggagtac
      301 agcgccatgc gggaccagta catgcgcacc ggggagggct tcctgtgtgt gtttgccatc
      361 aacaacacca agtcttttga ggacatccac cagtacaggg agcagatcaa acgggtgaag
      421 gactcggatg acgtgcccat ggtgctggtg gggaacaagt gtgacctggc tgcacgcact
      481 gtggaatctc ggcaggctca ggacctcgcc cgaagctacg gcatccccta catcgagacc
      541 tcggccaaga cccggcaggg agtggaggat nccttctaca cgttggtgcg tgagatccgg
      601 cagcacaagc tgcggaagct gaaccctcct gatgagagtg gccccggctg catgagctgc
      661 aagtgtgtgc tctcctgatt gccaactttc ttgtacaaag ttggcattat aagaaagcat
      721 tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc
      781 catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag
      841 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
      901 tgcct
//
