LOCUS       sequence-355326-       10140 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sars-CoV-2 variant protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10140)
  AUTHORS   Liu X, Huuskonen S, Laitinen T, Redchuk T, Bogacheva M, Salokas K, 
            Pohner I, Ohman T, Tonduru AK, Hassinen A, Gawriyski L, Keskitalo S,
            Vartiainen MK, Pietiainen V, Poso A, Varjosalo M
  TITLE     SARS-CoV-2-host proteome interactions for antiviral drug discovery.
  JOURNAL   Mol Syst Biol. 2021 Nov;17(11):e10396. doi: 10.15252/msb.202110396.
  PUBMED    34709727
REFERENCE   2  (bases 1 to 10140)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2021-11"; volume: "17(11):e10396. doi"; pages: " 
            10.15252/msb.202110396"
COMMENT     Sequence Label: MAC-C SARS-CoV-2 Omicron spike protein
FEATURES             Location/Qualifiers
     source          1..10140
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..46)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(200..788)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(280..299)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(959..1750,1751..1819))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1582..1601
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1820..1924)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1999..2018)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2190..2569
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2570..2773
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2724..2744
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2775..2793
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2796..2814
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2824..2848
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2991..3015
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6832..6856)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6903..6926
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6963..6986
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             7017..7043
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             7017..7043
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(7044..7391,7392..7394,7395..8003)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(8047..8064)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8053..8277
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8410..8429)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    8561..8608
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             8616..9638
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             8616..9638
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    9768..9889
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9805..9824)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9859..9878
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9938..9954)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9938..9954)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9951..9973)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9962..9978
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9986..9992,9993..10010,10011..10016))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10031..10052
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
       61 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      121 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      181 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
      241 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      301 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      361 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
      421 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
      481 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
      541 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
      601 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
      661 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
      721 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
      781 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
      841 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
      901 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
      961 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     1021 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     1081 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     1141 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     1201 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     1261 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     1321 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     1381 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     1441 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     1501 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     1561 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     1621 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     1681 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     1741 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     1801 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     1861 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     1921 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc
     1981 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
     2041 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
     2101 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
     2161 tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta
     2221 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     2281 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     2341 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     2401 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     2461 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     2521 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     2581 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     2641 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     2701 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     2761 tataagcaga gctctcccta tcagtgatag agatctccct atcagtgata gagatcgtcg
     2821 acgagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     2881 ccatagaaga caccgggacc gatccagcct ccggactcta gcgtttaaac ttaagcttgg
     2941 taccgagctc ggatccacta gtccagtgtg gtggaattct gcagatatca acaagtttgt
     3001 acaaaaaagc aggctccacc atgttcgtgt ttctggtcct gctgcctctg gtctcatcac
     3061 agtgcgtcaa tctgacaact cggactcagc tgccccctgc ctatactaac agctttacac
     3121 ggggcgtcta ctatcccgac aaggtgttca gaagtagcgt cctgcatagc actcaggacc
     3181 tgttcctgcc cttcttctcc aacgtgactt gtttccatgt catctccggg accaatggga
     3241 ccaaacggtt tgacaaccca gtcctgccat tcaatgatgg ggtctatttt gctagcatcg
     3301 aaaagagtaa tatcattaga gggtggatct ttggaaccac actggatagc aaaacccagt
     3361 ccctgctgat cgtcaacaac gcaactaacg tggtcattaa ggtctgtgag ttccagttct
     3421 gcaatgatcc tttcctggac cacaaaaaca ataagagttg gatggaatct gagttcagag
     3481 tctattcaag tgctaataac tgcaccttcg aatacgtgtc acagccattc ctgatggatc
     3541 tggaaggaaa gcagggcaac ttcaagaatc tgcgggagtt tgtcttcaag aacatcgatg
     3601 gatatttcaa gatctatagc aagcataccc ctatcattgt gcgggagcca gaagatctgc
     3661 cccagggctt tagcgccctg gaacctctgg tcgatctgcc tatcgggatt aacatcactc
     3721 ggtttcagac tctgctggcc ctgcatcgga gttacctgac accaggagat agcagttctg
     3781 gctggaccgc cggcgccgct gcatattatg tgggctatct gcagccacgc acattcctgc
     3841 tgaaatacaa cgaaaatggg actattacag acgctgtcga ctgcgcactg gaccctctgt
     3901 cagaaacaaa atgtactctg aaatctttca cagtggaaaa aggcatctat cagacatcta
     3961 acttccgcgt gcagccaact gaatctatcg tgagatttcc taatattact aatctgtgcc
     4021 cattcgatga ggtgtttaat gctacccggt ttgcctccgt gtatgcatgg aaccggaaaa
     4081 ggattagcaa ttgtgtggct gattatagcg tgctgtataa tctggcacct ttcttcacct
     4141 tcaagtgtta cggagtgtca cccacaaagc tgaatgacct gtgctttacc aatgtctacg
     4201 cagactcttt cgtgattcgc ggcgacgagg tgcgccagat cgcaccagga cagaccggca
     4261 atatcgcaga ttacaattat aagctgcctg acgactttac agggtgcgtg atcgcttgga
     4321 acagtaacaa actggacagc aaagtgagcg gaaactataa ctatctgtat cgcctgttca
     4381 gaaaatcaaa tctgaaaccc ttcgaaagag atatttccac tgaaatttac caggcaggaa
     4441 ataagccctg caacggggtg gctgggttca attgttattt tccactgaga tcttactcat
     4501 tccgccccac atacggagtg ggccatcagc cttaccgggt ggtggtgctg agctttgaac
     4561 tgctgcacgc accagctaca gtctgcggac ccaagaaatc cactaacctg gtcaagaaca
     4621 aatgcgtgaa tttcaatttc aatgggctga agggcactgg ggtgctgaca gagtcaaata
     4681 agaagtttct gcctttccag cagttcggac gcgacatcgc tgacactaca gatgcagtcc
     4741 gggaccctca gaccctggag attctggata tcactccatg ttccttcgga ggcgtctctg
     4801 tgatcacacc cggaactaat accagtaacc aggtcgcagt gctgtaccag ggcgtgaact
     4861 gcaccgaggt gcctgtggcc attcacgcag atcagctgac ccccacatgg cgcgtctaca
     4921 gtactggctc caatgtcttt cagactcggg caggctgtct gattggagct gagtacgtga
     4981 acaactctta cgagtgtgac atcccaatcg gagctggcat ctgtgccagt taccagaccc
     5041 agaccaagag tcatcggcgg gcccgaagtg tggcttctca gagcatcatc gcctatacta
     5101 tgtctctggg cgctgagaat agtgtggctt actcaaataa tagtatcgct attcccacaa
     5161 acttcactat ctctgtgacc acagaaattc tgccagtgtc aatgaccaag acaagtgtgg
     5221 actgcaccat gtatatttgc ggcgatagca ctgagtgctc aaacctgctg ctgcagtatg
     5281 ggtcattctg cacccagctg aaacgcgcac tgacaggcat tgctgtcgag caggacaaga
     5341 atacccagga agtctttgca caggtgaaac agatctataa aacaccacct atcaagtact
     5401 tcgggggctt taacttctct cagattctgc ctgacccaag caaaccatcc aagaggagct
     5461 tcatcgagga tctgctgttc aacaaagtca ctctggctga tgctggattc attaagcagt
     5521 acggagattg tctgggcgac attgcagcaa gagatctgat ctgtgctcag aagttcaagg
     5581 gcctgacagt cctgccacca ctgctgacag atgaaatgat cgcacagtat acttccgccc
     5641 tgctggctgg caccatcact agcgggtgga cattcggagc cggagctgcc ctgcagatcc
     5701 ccttcgccat gcagatggca tacaggttca acggaatcgg ggtcactcag aatgtcctgt
     5761 atgaaaatca gaagctgatt gctaaccagt ttaattccgc catcggcaag attcaggatt
     5821 ctctgagttc taccgcctct gctctgggaa agctgcagga tgtggtcaat cataatgcac
     5881 aggcactgaa caccctggtg aaacagctgt cctctaagtt cggcgctatt tcttcagtcc
     5941 tgaacgatat tttctctcgg ctggataagg tggaggctga ggtccagatt gatagactga
     6001 tcactggacg gctgcagtca ctgcagacct acgtcactca gcagctgatt cgggccgctg
     6061 aaatcagagc atctgcaaac ctggctgcca caaaaatgag tgagtgcgtg ctggggcaga
     6121 gcaagagagt cgacttctgc gggaaaggat accatctgat gagtttccca cagtctgctc
     6181 cacatggagt ggtctttctg catgtcacat acgtccccgc tcaggagaag aatttcacaa
     6241 ctgcacccgc tatttgccac gatgggaagg cacactttcc ccgcgaagga gtcttcgtgt
     6301 ccaatggaac tcactggttc gtgactcagc gaaacttcta cgaaccacag attatcacaa
     6361 ctgataatac attcgtgtca ggcaattgcg acgtggtcat tggcatcgtc aacaatacag
     6421 tgtacgatcc tctgcagcca gagctggata gtttcaaaga ggaactggat aaatacttta
     6481 agaaccacac ctcacctgat gtggacctgg gggacatctc tgggatcaat gcctctgtcg
     6541 tgaatattca gaaggaaatc gaccggctga atgaggtcgc taaaaacctg aatgagtctc
     6601 tgattgacct gcaggagctg ggcaagtacg aacagtatat caagtggcca tggtacatct
     6661 ggctggggtt catcgcagga ctgattgcta tcgtgatggt gacaatcatg ctgtgctgca
     6721 tgactagttg ttgctcctgt ctgaaaggct gttgttcctg cgggtcttgt tgtaaattcg
     6781 acgaggatga ttctgaaccc gtgctgaagg gagtcaaact gcattatacc gacccagctt
     6841 tcttgtacaa agtggttgat atcaaggccg ccgacatcac cagcctgtac aagaaagtgg
     6901 ggtggagcca tccccagttc gagaagggcg gaggatctgg cggcggaagc ggcggaggaa
     6961 gctggtccca ccctcagttt gagaaaggca ccgagctggg cagcaccatg gccagctacc
     7021 cctacgacgt gcccgactac gccaaggaca acaccgtgcc cctgaagctg atcgccctgc
     7081 tggccaacgg cgagttccac tctggcgagc agctgggcga gacactgggc atgagcagag
     7141 ccgccatcaa caagcacatc cagaccctgc gggactgggg cgtggacgtg ttcacagtgc
     7201 ccggcaaggg ctacagcctg cccgagccca tccagctgct gaacgccaag cagatcctgg
     7261 gccagctgga tggcggatct gtggccgtgc tgcccgtgat cgacagcacc aaccagtatc
     7321 tgctcgaccg gatcggcgag ctgaagtccg gcgacgcctg tatcgccgag taccagcagg
     7381 ccggcagagg cggcaggggc agaaagtggt tcagcccctt cggcgccaac ctgtacctga
     7441 gcatgttctg gagactggaa cagggcccag ccgccgctat tggcctgagc ctggtcatcg
     7501 gcatcgtgat ggccgaggtg ctgcggaagc tgggcgccga caaagtgcgc gtgaagtggc
     7561 ccaacgacct gtatctgcag gaccggaagc tggccggcat cctggtggaa ctgaccggca
     7621 aaacaggcga cgccgcccag atcgtgatcg gcgctggcat caacatggcc atgcggcggg
     7681 tggaagagag cgtggtcaac cagggctgga tcaccctgca ggaagccggc atcaacctgg
     7741 accggaacac cctggccgcc atgctgatca gagagctgag agccgccctg gaactgttcg
     7801 agcaggaagg cctggccccc tacctgagca gatgggagaa gctggacaac ttcatcaacc
     7861 ggcccgtgaa actgatcatc ggcgacaaag agatcttcgg catcagccgg ggcatcgaca
     7921 agcagggggc cctgctcctg gaacaggacg gcatcatcaa gccctggatg ggcggcgaga
     7981 tcagcctgcg gagcgccgag aagtgatgac tcgagtctag agggcccgtt taaacccgct
     8041 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     8101 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     8161 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     8221 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     8281 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     8341 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     8401 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     8461 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     8521 accccaaaaa acttgattag ggtgatggtt cacgtaccta gaagttccta ttccgaagtt
     8581 cctattctct agaaagtata ggaacttcct tggccaaaaa gcctgaactc accgcgacgt
     8641 ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg
     8701 agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg
     8761 taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg
     8821 ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt
     8881 gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg
     8941 ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga
     9001 cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt
     9061 tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg
     9121 tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg
     9181 aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc
     9241 gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg
     9301 ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg
     9361 agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg
     9421 gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc
     9481 agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg
     9541 cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa
     9601 accgacgccc cagcactcgt ccgagggcaa aggaatagca cgtactacga gatttcgatt
     9661 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     9721 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     9781 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     9841 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     9901 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     9961 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
    10021 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
    10081 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
//
