LOCUS       sequence-355333-       10695 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for expression of Human Transferrin receptor-1 
            fused mApple.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10695)
  AUTHORS   Rennick JJ, Nowell CJ, Pouton CW, Johnston APR
  TITLE     Resolving subcellular pH with a quantitative fluorescent lifetime 
            biosensor.
  JOURNAL   Nat Commun. 2022 Oct 12;13(1):6023. doi: 10.1038/s41467-022-33348-z.
  PUBMED    36224168
REFERENCE   2  (bases 1 to 10695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-10-12"; volume: "13(1):6023. doi"; pages: " 
            10.1038/s41467-022-33348-z"
COMMENT     Sequence Label: pCDH-TfR-mApple
FEATURES             Location/Qualifiers
     source          1..10695
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..232
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1496..1540
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1807..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1966..2177
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2190..2458
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             4849..5559
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     5262..5281
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    join(5569..6143,6144..6146,6147..6155)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5736..5753)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5963..5982
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6168..6767
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6168..6187)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6664..6684
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6774..7362
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6827..6847)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7245..7256)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7436..7669
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7741..7862
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7778..7797)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7832..7851
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7902..8037
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8070..8086)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8070..8086)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8083..8105)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8094..8110
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8118..8124,8125..8142,8143..8148))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8163..8184
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8301..8318)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8472..9060)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(9231..10022,10023..10091))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9854..9873
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10092..10196)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10264..10282
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10320..10342)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10442..10461
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10655..10677
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10669..10686
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10670..10686
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
     1921 tttatcgata ctagtggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat
     1981 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa
     2041 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
     2101 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
     2161 ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc
     2221 gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg
     2281 gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc
     2341 tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga
     2401 ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca
     2461 agctgtgacc ggcgcctact ctagagctag cgctaccgga ctcagatctc gagcgccacc
     2521 atgatggatc aagctagatc agcattctct aacttgtttg gtggagaacc attgtcatat
     2581 acccggttca gcctggctcg gcaagtagat ggcgataaca gtcatgtgga gatgaaactt
     2641 gctgtagatg aagaagaaaa tgctgacaat aacacaaagg ccaatgtcac aaaaccaaaa
     2701 aggtgtagtg gaagtatctg ctatgggact attgctgtga tcgtcttttt cttgattgga
     2761 tttatgattg gctacttggg ctattgtaaa ggggtagaac caaaaactga gtgtgagaga
     2821 ctggcaggaa ccgagtctcc agtgagggag gagccaggag aggacttccc tgcagcacgt
     2881 cgcttatatt gggatgacct gaagagaaag ttgtcggaga aactggacag cacagacttc
     2941 accagcacca tcaagctgct gaatgaaaat tcatatgtcc ctcgtgaggc tggatctcaa
     3001 aaagatgaaa atcttgcgtt gtatgttgaa aatcaatttc gtgaatttaa actcagcaaa
     3061 gtctggcgtg atcaacattt tgttaagatt caggtcaaag acagcgctca aaactcggtg
     3121 atcatagttg ataagaacgg tagacttgtt tacctggtgg agaatcctgg gggttatgtg
     3181 gcgtatagta aggctgcaac agttactggt aaactggtcc atgctaattt tggtactaaa
     3241 aaagattttg aggatttata cactcctgtg aatggatcta tagtgattgt cagagcaggg
     3301 aaaatcacct ttgcagaaaa ggttgcaaat gctgaaagct taaatgcaat tggtgtgttg
     3361 atatacatgg accagactaa atttcccatt gttaacgcag aactttcatt ctttggacat
     3421 gctcatctgg ggacaggtga cccttacaca cctggattcc cttccttcaa ccacactcag
     3481 tttccaccat ctcggtcatc aggattgcct aatatacctg tccagacaat ctccagagct
     3541 gctgcagaaa agctgtttgg gaatatggaa ggagactgtc cctctgactg gaaaacagac
     3601 tctacatgta ggatggtaac ctcagaaagc aagaatgtga agctcactgt gagcaatgtg
     3661 ctgaaagaga taaaaattct taacatcttt ggagttatta aaggctttgt agaaccagat
     3721 cactatgttg tagttggggc ccagagagat gcatggggcc ctggagctgc aaaatccggt
     3781 gtaggcacag ctctcctatt gaaacttgcc cagatgttct cagatatggt cttaaaagat
     3841 gggtttcagc ccagcagaag cattatcttt gccagttgga gtgctggaga ctttggatcg
     3901 gttggtgcca ctgaatggct agagggatac ctttcgtccc tgcatttaaa ggctttcact
     3961 tatattaatc tggataaagc ggttcttggt accagcaact tcaaggtttc tgccagccca
     4021 ctgttgtata cgcttattga gaaaacaatg caaaatgtga agcatccggt tactgggcaa
     4081 tttctatatc aggacagcaa ctgggccagc aaagttgaga aactcacttt agacaatgct
     4141 gctttccctt tccttgcata ttctggaatc ccagcagttt ctttctgttt ttgcgaggac
     4201 acagattatc cttatttggg taccaccatg gacacctata aggaactgat tgagaggatt
     4261 cctgagttga acaaagtggc acgagcagct gcagaggtcg ctggtcagtt cgtgattaaa
     4321 ctaacccatg atgttgaatt gaacctggac tatgagaggt acaacagcca actgctttca
     4381 tttgtgaggg atctgaacca atacagagca gacataaagg aaatgggcct gagtttacag
     4441 tggctgtatt ctgctcgtgg agacttcttc cgtgctactt ccagactaac aacagatttc
     4501 gggaatgctg agaaaacaga cagatttgtc atgaagaaac tcaatgatcg tgtcatgaga
     4561 gtggagtatc acttcctctc tccctacgta tctccaaaag agtctccttt ccgacatgtc
     4621 ttctggggct ccggctctca cacgctgcca gctttactgg agaacttgaa actgcgtaaa
     4681 caaaataacg gtgcttttaa tgaaacgctg ttcagaaacc agttggctct agctacttgg
     4741 actattcagg gagctgcaaa tgccctctct ggtgacgttt gggacattga caatgagttt
     4801 tcagaattcg gctccaccgg ctccaccggc tccaccggcg cggatccaat ggtgagcaag
     4861 ggcgaggaga ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
     4921 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     4981 gcctttcaga ccgctaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     5041 atcctgtccc ctcagttcat gtacggctcc aaggtctaca ttaagcaccc agccgacatc
     5101 cccgactact tcaagctgtc cttccccgag ggcttcaggt gggagcgcgt gatgaacttc
     5161 gaggacggcg gcattattca cgttaaccag gactcctccc tgcaggacgg cgtgttcatc
     5221 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
     5281 accatgggct gggaggcctc cgaggagcgg atgtaccccg aggacggcgc cctgaagagc
     5341 gagatcaaga agaggctgaa gctgaaggac ggcggccact acgccgccga ggtcaagacc
     5401 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acatcgtcga catcaagttg
     5461 gacatcgtgt cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     5521 cgccactcca ccggcggcat ggacgagctg tacaagtaag cggccgcgcc cctctccctc
     5581 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
     5641 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
     5701 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
     5761 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
     5821 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
     5881 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
     5941 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
     6001 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac
     6061 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt
     6121 cctttgaaaa acacgatgat aatatggcca caaccatggc gtccggaatg accgagtaca
     6181 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     6241 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     6301 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     6361 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     6421 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     6481 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     6541 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     6601 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     6661 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     6721 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctaggtc gacaatcaac
     6781 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     6841 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     6901 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     6961 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     7021 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     7081 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     7141 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     7201 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     7261 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     7321 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggtacc tttaagacca
     7381 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     7441 gggctaattc actcccaacg aagataagat ctgctttttg cttgtactgg gtctctctgg
     7501 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     7561 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     7621 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     7681 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     7741 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     7801 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     7861 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
     7921 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     7981 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     8041 acttttgcag agaccaaatt cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat
     8101 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     8161 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     8221 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     8281 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     8341 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     8401 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     8461 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     8521 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     8581 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8641 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     8701 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8761 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8821 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8881 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     8941 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     9001 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     9061 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     9121 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     9181 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     9241 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     9301 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     9361 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     9421 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     9481 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     9541 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     9601 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     9661 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     9721 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     9781 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     9841 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     9901 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     9961 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    10021 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    10081 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    10141 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    10201 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
    10261 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
    10321 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
    10381 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
    10441 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
    10501 tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg
    10561 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
    10621 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
    10681 gccagtgcca agctg
//
