LOCUS       sequence-35537-e         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller 
            JA, Somia NV, Bogdanove AJ, Voytas DF
  TITLE     Efficient design and assembly of custom TALEN and other TAL 
            effector-based constructs for DNA targeting.
  JOURNAL   Nucleic Acids Res. 2011 Jul;39(12):e82. doi: 10.1093/nar/gkr218
  PUBMED    21493687
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-07"; volume: "39(12):e82. doi"; pages: " 
            10.1093/nar/gkr21"
COMMENT     Sequence Label: pNK5
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(9..25)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9..25)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      304..891
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     793..812
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt
       61 acattgcaca agataaaaat atatcatcat gcctcctcta gaggtctcct gttgccggtg
      121 ctgtgccagg accatggcct gaccccggac caagtggtgg ctatcgccag caacaagggc
      181 ggcaagcaag cgctcgaaac ggtgcaggag accctcgagc cacccatgac caaaatccct
      241 taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
      301 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
      361 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
      421 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
      481 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
      541 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
      601 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
      661 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
      721 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
      781 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
      841 acttgagcgt cgatttttgt gatgctcgtc agggggcgga gcctatggaa aaacgccagc
      901 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
//
