LOCUS       sequence-355411-        2604 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-CT2
FEATURES             Location/Qualifiers
     source          1..2604
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     380..399
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(606..611,612..628,629..634)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             682..1872
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1034..1053)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2046..2065)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2047..2065)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2070..2086)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2070..2086)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      join(2495..2604,1..479)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
       61 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
      121 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
      181 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
      241 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
      301 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
      361 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
      421 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
      481 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
      541 tctttcctgc gttatccaca gaatcagggg ataacgcagg aaagaacatg aattaattct
      601 catgtttgac agcttatcat cgattagctt taatgcggta gtttatcaca gttaaattgc
      661 taacgcagtc aggcaccgtg tatgaaatct aacaatgcgc tcatcgtcat cctcggcacc
      721 gtcaccctgg atgctgtagg cataggcttg gttatgccgg tactgccggg cctcttgcgg
      781 gatatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc gctatatgcg
      841 ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt tggccgccgc
      901 ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat ggcgaccaca
      961 cccgtcctgt ggattctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
     1021 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
     1081 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
     1141 ttgggcgcca tctccttaca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
     1201 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgccg acccatgccc
     1261 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc
     1321 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg
     1381 gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg
     1441 gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt
     1501 ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg
     1561 ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc
     1621 ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag
     1681 ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcat tggaccgctg
     1741 atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc atggattgta
     1801 ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc
     1861 acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccact ccaagaattg
     1921 gagccaatca attcttgcgg agaactgtga atgcgcaaac caacccttga tcggggaaga
     1981 acagtatgtc gagctatttt ttgacttact ggggatcaag cctgattggg agaaaataaa
     2041 atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg
     2101 tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca tgcctcctct
     2161 agaccaaagc tggtctcgca ggttgtagcg attgctagtc atgacggtgg caaacaggct
     2221 cttgaaaccg tccaacgcct tctaccagtt ctctgtcaag cccacggact aaccccagcg
     2281 caagttgtag cgattgctag taatgggggt ggcaaacagg ctcttgagac tgttcagcgc
     2341 cttctaccag ttctctgtca agcccacggc ctgacgcccg agcaagtggt tgagaccata
     2401 tctggtcgag ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag
     2461 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     2521 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     2581 agctaccaac tctttttccg aagg
//
