LOCUS       sequence-355412-        5654 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5654)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_5277:Dendra
FEATURES             Location/Qualifiers
     source          1..5654
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     733..752
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        733..751
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2285..2308
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2565..3365
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3490..4078
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3979..3998
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 caagggcccc gaggcattcc tggtgaccac gacgcagggc aaccggctgt cgaagtccgc
       61 gttcaccaag tcgctgaagc gtggctacgc caagatcggt cggccggaac tccgcatcca
      121 cgacctccgc gctgtcggcg ctacgttcgc cgctcaggca ggtgcgacga ccaaggagct
      181 gatggcccgt ctcggtcaca cgactcctag gatggcgatg aagtaccaga tggcgtctga
      241 ggcccgcgac gaggctatcg ctgaggcgat gtccaagctg gccaagacct cctgaaacgc
      301 aaaaagcccc cctcccaagg acactgagtc ctaaagaggg gggtttcttg tcagtacgcg
      361 aagaaccacg cctggccgcg agcgccagca ccgccgctct gtgcggagac ctgggcacca
      421 gccccgccgc cgccaggagc attgccgttc ccgccagaaa tctagacggt gaccacaacg
      481 cgcccgcttt gatcggggac gtcgaaatat tggatcgtcg gcaccgtcac ggccgtggga
      541 ggcggcacga tccgcgacgt gatgatcggc cgcatcccca cggtgctgcg cagtgagctc
      601 tacgccatcc cggcgttgat ctgtgcgttc gcacgcacag gcccggtgtg agaagggtct
      661 ctgacgagcg ggagaactcc ctatcagtga tagagtttgt cctccctatc agtgatagat
      721 aggcttaagg agtaatacga ctcactatag ggagaaggag gccattatga gcgacttcga
      781 gacgatcctg gtgacccgca ccgaccgggt cgccaccatc cagctgaacc ggcccaaggc
      841 gctcaacgcg ctcaacagcc aggtgatgac ggaagtgacc gcggccgcag ccgaactcga
      901 caaggatccg ggcgtcggcg cgatcatcgt cacgggcaac gagaaggcct tcgccgcggg
      961 tgcggacatc aaggagatgg cggacctgtc gttcgccgag gtcttcgagg cggacttctt
     1021 cgaactgtgg tccaagttcg ccgccacccg cacgcccacc atcgccgcgg tcgccgggta
     1081 cgccctcggc ggcggctgcg agctcgcgat gatgtgtgac atcctgatcg ccgccgacac
     1141 cgcgaaattc gggcagcccg agatcaagct cggcgtgctc cccggaatgg gcggttcgca
     1201 acggctcacc cgcgcgatcg gcaaggccaa ggccatggac ctcatcctca ccggacgcac
     1261 catcgacgcc gccgaggccg aacgggccgg gctggtctcg cgtctggtgc ccgcggacac
     1321 gctgatcgac gaggcgctcg cggtggcgca gaccatcgcc gggatgtcgc tgtcggcgtc
     1381 gcgcatggcc aaggaagccg tcaaccgcgc gttcgagacc agcctcaccg agggtctgct
     1441 ctacgagcgc aggctgttcc actccgcttt cgccaccgcc gaccagaaag agggcatggc
     1501 cgcgtttgcc gagaagcgcg ccgccaactt cacgcatcgc aagcttggtg cggccgcggg
     1561 caacacgcct ggcatcaatc tgatcaagga ggacatgcgt gtcaaggtgc atatggaggg
     1621 caacgtgaac ggtcacgcct tcgtgatcga gggggagggt aagggtaagc cttacgaagg
     1681 gacccagacc gccaacctca ccgtcaagga gggcgcccct ctcccattct cgtacgatat
     1741 cctcactacc gcggtgcatt acgggaaccg cgtgttcacc aagtaccccg aggatatccc
     1801 tgactacttc aagcagagct tcccggaggg atactcctgg gagcggacga tgaccttcga
     1861 agacaagggt atctgtacga tccgcagcga catctcgctg gaaggggact gtttcttcca
     1921 gaacgtccgt ttcaagggga ccaactttcc gccgaacggc ccggtcatgc agaagaagac
     1981 cctcaagtgg gagccgagca cggagaagct gcacgttcgg gacggcctcc tggtcggcaa
     2041 catcaacatg gcgctgctgc tggaaggcgg cggccactac ctatgcgact tcaagaccac
     2101 gtacaaagcg aagaaggtcg tccagctgcc cgacgcgcac ttcgtggacc accgcatcga
     2161 aatcctgggg aacgattcgg actacaacaa ggtcaagttg tacgagcacg ctgtggcacg
     2221 ctatagcccg ctcccgtcgc aggtctggtt cgaaggcggc ggcggggaga acctgtattt
     2281 ccaggactac aaggacgacg acgacaagtg atttaaataa ccccttgggg cctctaaacg
     2341 ggtcttgagg ggttttttgt taactagcgt acgatcgaat tcctgcagct cacggtaact
     2401 gatgccgtat ttgcagtacc agcgtacggc ccacagaatg atgtcacgct gaaaatgccg
     2461 gcctttgaat gggttcatgt gcagctccat cagcaaaagg ggatgataag tttatcacca
     2521 ccgactattt gcaacagtgc cgttgatcgt gctatgatcg actgatgtca tcagcggtgg
     2581 agtgcaatgt cgtgcaatac gaatggcgaa aagccgagct catcggtcag cttctcaacc
     2641 ttggggttac ccccggcggt gtgctgctgg tccacagctc cttccgtagc gtccggcccc
     2701 tcgaagatgg gccacttgga ctgatcgagg ccctgcgtgc tacgctgggt ccgggaggga
     2761 cgctcgtcat gccctcgtgg tcaggtctgg acgacgagcc gttcgatcct gccacgtcgc
     2821 ccgttacacc ggaccttgga gttgtctctg acacattctg gcgcctgcca aatgtaaagc
     2881 gcagcgccca tccatttgcc tttgcggcag cggggccaca ggcagagcag atcatctctg
     2941 atccattgcc cctgccacct tactcgcctg caagcccggt cgcccgtgtc catgaactcg
     3001 atgggcaggt acttctcctc ggcgtgggac acgatgccaa cacgacgctg catcttgccg
     3061 agttgatggc aaaggttccc tatggggtgc cgagacactg caccattctt caggatggca
     3121 agttggtacg cgtcgattat ctcgagaatg accactgctg tgagcgcttt gccttggcgg
     3181 acaggtggct caaggagaag agccttcaga aggaaggtcc agtcggtcat gcctttgctc
     3241 ggttgatccg ctcccgcgac attgtggcga cagccctggg tcaactgggc cgagatccgt
     3301 tgatcttcct gcatccgcca gaggcgggat gcgaagaatg cgatgccgct cgccagtcga
     3361 ttggctgagc tcatgagcgg agaacgagat gacgttggag gggcaaggtc gcgctgattg
     3421 ctggggcaac acgggggatc cactagttcc atgagcgtca gaccccgtag aaaagatcaa
     3481 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3541 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     3601 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     3661 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     3721 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     3781 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     3841 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     3901 tcccgagggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     3961 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4021 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4081 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     4141 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     4201 taccgctcgc cgcagccgaa cgaccgagcg caacgcgtgc ggccgcggta cccggggatc
     4261 ctctagagtc gaccaccaag ggcaccatct ctgcttgggc caccccgttg gccgcagcca
     4321 gctcgctgag agccgtgaac gacagggcga acgccagccc gccgacggcg agggttccga
     4381 ccgctgcaac tcccggtgca accttgtccc ggtctattct cttcactgca ccagctccaa
     4441 tctggtgtga atgcccctcg tctgttcgcg caggcggggg gctctattcg tttgtcagca
     4501 tcgaaagtag ccagatcagg gatgcgttgc aaccgcgtat gcccaggtca gaagagtcgc
     4561 acaagagttg cagacccctg gaaagaaaaa tggccagagg gcgaaaacac cctctgacca
     4621 gcggagcggg cgacgggaat cgaacccgcg tagctagttt ggaagaatgg gtgtctgccg
     4681 accacatatg ggccggtcaa gataggtttt taccccctct cggctgcatc ctctaagtgg
     4741 aaagaaattg caggtcgtag aagcgcgttg aagcctgaga gttgcacagg agttgcaacc
     4801 cggtagcctt gttcacgacg agaggagacc tagttggcac gtcgcggatg gggatcgctg
     4861 aagactcagc gcagcgggag gatccaagcc tcatacgtca acccgcagga cggtgtgagg
     4921 tactacgcgc tgcagaccta cgacaacaag atggacgccg aagcctggct cgcgggcgag
     4981 aagcggctca tcgagatgga gacctggacc cctccacagg accgggcgaa gaaggcagcc
     5041 gccagcgcca tcacgctgga ggagtacacc cggaagtggc tcgtggagcg cgacctcgca
     5101 gacggcacca gggatctgta cagcgggcac gcggagcgcc gcatctaccc ggtgctaggt
     5161 gaagtggcgg tcacagagat gacgccagct ctggtgcgtg cgtggtgggc cgggatgggt
     5221 aggaagcacc cgactgcccg ccggcatgcc tacaacgtcc tccgggcggt gatgaacaca
     5281 gcggtcgagg acaagctgat cgcagagaac ccgtgccgga tcgagcagaa ggcagccgat
     5341 gagcgcgacg tagaggcgct gacgcctgag gagctggaca tcgtcgccgc tgagatcttc
     5401 gagcactacc ggatcgcggc atacatcctg gcgtggacga gcctccggtt cggagagctg
     5461 atcgagcttc gccgcaagga catcgtggac gacggcatga cgatgaagct ccgggtgcgc
     5521 cgtggcgctt cccgcgtggg gaacaagatc gtcgttggca acgccaagac cgtccggtcg
     5581 aagcgtcctg tgacggttcc gcctcacgtc gcggagatga tccgagcgca catgaaggac
     5641 cgtacgaaga tgaa
//
