LOCUS       sequence-355413-        8030 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of HsAGO2-F2V2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8030)
  AUTHORS   Eulalio A, Huntzinger E, Izaurralde E
  TITLE     GW182 interaction with Argonaute is essential for miRNA-mediated 
            translational repression and mRNA decay.
  JOURNAL   Nat Struct Mol Biol. 2008 Apr . 15(4):346-53.
  PUBMED    18345015
REFERENCE   2  (bases 1 to 8030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2008 Apr . 15(4):346-53."
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-HsAGO2-F2V2_F
FEATURES             Location/Qualifiers
     source          1..8030
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..2510
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2530..2595
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2620..2646
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      4591..4600
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5281..5322
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5332..5349
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5372..5389)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5422..5556
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5446..5465)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5500..5519
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6157..6745)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6237..6256)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6916..7707,7708..7776))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7539..7558
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7777..7881)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7949..7967
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 catgaatggc atcaactctg aatcaaatct ttgcagatgc acctacttct catttccact
       61 gtcacatcat ttttccagat ctcgctgcct gttatgtggc ccacaaacca agacacgttt
      121 tatggccatt aaagctggct gatcgtcgcc aaacaccaaa tacataatga atatgtacac
      181 attcgagaaa gaagcgatca aagaagcgtc ttcgggcgga gtaggagaat gcggaggaga
      241 aggagaacga gctgatctag tatctctcca caatccaatg ccaactgacc aactggccat
      301 attcggagca atttgaagcc aatttccatc gcctggcgat cgctccattc ttggctatat
      361 gtttttcacc gttacccggg gccattttca aagactcgtc ggcaagataa gattgtgtca
      421 ctcgctgtct ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag
      481 tattttgaag aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac
      541 aaaaacatgt tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt
      601 atgtatatac ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac
      661 tgatgatatt ttaagatgat gccagaccaa aaggcttgaa tttctgcgtc ttttgccgaa
      721 cgcagtgcat gtgcaattgt tgttttttgg aatattcaat tttcggactg tccgctttga
      781 tttcagtttc ttggcttatt caaaaagcaa agtaaagcca aaaaagcgag atggcaatac
      841 caaatgcggc aaaacggtag tggaaggaaa ggggtgcggg gcagcggaag gaagggtggg
      901 gcggggcgtg gcggggtctg tggctgggcg cgacgtcacc gacgttggag ccactccttt
      961 gaccatgtgt gcgtgtgtgt attattcgtg tctcgccact cgccggttgt ttttttcttt
     1021 ttatgctgcg ctctctctag cgccatctcg cttacgcatg ctcaacgcac cgcatgttgc
     1081 cgtttccttt tatgcgtcat tttggctcga aataggcaat tatttaaaca aagattagtc
     1141 aacgaaaacg ctaaaataaa taagtctaca atatggttac ttattgccat gtgtgtgcag
     1201 ccaacgatag caacaaaagc aacaacacag gtggctttcc ctctttcact ttttgtttgc
     1261 aagccgcgtg cgagcaagac ggcacgaccg gcaaacgcaa ttacgctgac aaagagcaga
     1321 cgaagttttg gcgaaaaaca tcaaggcgcc tgatacgaat gcatttgcaa taacaattgc
     1381 gatatttaat attgtttatg aagctgtttg acttcaaaac acacaaaaaa aaaaataaaa
     1441 caaattattt gaaagagaat taggaatcgg acgcttatcg ttagggtaac aacaagaaat
     1501 gcttactgag tcacagcctc tggaaaactg ccgcaagcca gagagagaga gaaaaagagg
     1561 gagagcagct tagaccgcat gtgcttgtgt gtgaggcgtc tctctcttcg tctctgttgc
     1621 gcaaacgcat agactgcact gaaaaaatcg attacctatt ttttatgaat gaatatttgc
     1681 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1741 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1801 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1861 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgaatgcc ctactagaag
     1921 atgtgtgttg cacaaaatgt ccctggaata accaatttga agtgcagata gcagtaaacg
     1981 taagctaata tgaatattat ttaactgtaa tgttttaata tcgctggaca ttactaataa
     2041 acccactata aacacatgta catatgtatg ttttggcata caatgagtag ttggggaaaa
     2101 aatgtgtaaa agcaccgtga ccatcacagc ataaagataa ccagctgaag tatcgaatat
     2161 gagtaacccc caaattgaat cacatgccgc aactgatagg acccatggaa gtacactctt
     2221 catggcgata tacaagacac acacaagcac gaacacccag ttgcggagga aattctccgt
     2281 aaatgaaaac ccaatcggcg aacaattcat acccatatat ggtaaaagtt ttgaacgcga
     2341 cttgagagcg gagagcattg cggctgataa ggttttagcg ctaagcgggc tttataaaac
     2401 gggctgcggg accagttttc atatcactac cgtttgagtt cttgtgctgt gtggatactc
     2461 ctcccgacac aaagccgctc catcagccag cagtcgtcta atccagagac cccggatcgg
     2521 ggtaccaaca tggacgcaca aacacgacga cgtgagcgtc gcgctgagaa acaagctcaa
     2581 tggaaagctg caaacggtgg atctcctcca catatggctt acccatacga tgttccagat
     2641 tacgctcctc caagcttgaa ttctatgtac tcgggagccg gccccgcact tgcacctcct
     2701 gcgccgccgc cccccatcca aggatatgcc ttcaagcctc cacctagacc cgactttggg
     2761 acctccggga gaacaatcaa attacaggcc aatttcttcg aaatggacat ccccaaaatt
     2821 gacatctatc attatgaatt ggatatcaag ccagagaagt gcccgaggag agttaacagg
     2881 gaaatcgtgg aacacatggt ccagcacttt aaaacacaga tctttgggga tcggaagccc
     2941 gtgtttgacg gcaggaagaa tctatacaca gccatgcccc ttccgattgg gagggacaag
     3001 gtggagctgg aggtcacgct gccaggagaa ggcaaggatc gcatcttcaa ggtgtccatc
     3061 aagtgggtgt cctgcgtgag cttgcaggcg ttacacgatg cactttcagg gcggctgcct
     3121 agcgtccctt ttgagacgat ccaggccctg gacgtggtca tgaggcactt gccatccatg
     3181 aggtacaccc ccgtgggccg ctccttcttc accgcgtccg aaggctgctc taaccctctt
     3241 ggcgggggcc gagaagtgtg gtttggcttc catcagtccg tccggccttc tctctggaaa
     3301 atgatgctga atattgatgt gtcagcaaca gcgttttaca aggcacagcc agtaatcgag
     3361 tttgtttgtg aagttttgga ttttaaaagt attgaagaac aacaaaaacc tctgacagat
     3421 tcccaaaggg taaagtttac caaagaaatt aaaggtctaa aggtggagat aacgcactgt
     3481 gggcagatga agaggaagta ccgcgtctgc aatgtgaccc ggcggcccgc cagtcaccaa
     3541 acattcccgc tgcagcagga gagcgggcag acggtggagt gcacggtggc ccagtatttc
     3601 aaggacaggc acaagcttgt tctgcgctac ccccacctcc catgtttaca agtcggacag
     3661 gagcagaaac acacctacct tcccctggag gtctgtaaca ttgtggcagg acaaagatgt
     3721 attaaaaaat taacggacaa tcagacctca accatgatca gagcaactgc taggtcggcg
     3781 cccgatcggc aagaagagat tagcaaattg atgcgaagtg caagtttcaa cacagatcca
     3841 tacgtccgtg aatttggaat catggtcaaa gatgagatga cagacgtgac tgggcgggtg
     3901 ctgcagccgc cctccatcct ctacgggggc aggaataaag ctattgcgac ccctgtccag
     3961 ggcgtctggg acatgcggaa caagcagttc cacacgggca tcgagatcaa ggtgtgggcc
     4021 attgcgtgct tcgcccccca gcgccagtgc acggaagtcc atctgaagtc cgtcacagag
     4081 cagctcagaa agatctcgag agacgctggc atgcccatcc agggccagcc gtgcttctgc
     4141 aaatacgcgc agggggcgga cagcgtggag cccatggtcc ggcacctgaa gaacacgtat
     4201 gcgggcctgc agctggtggt ggtcatcctg cccggcaaga cgcccgtgta cgccgaggtc
     4261 aagcgcgtgg gagacacggt gctggggatg gccacgcagt gcgtgcagat gaagaacgtg
     4321 cagaggacca cgccacagac cctgtccaac ctctgcctga agatcaacgt caagctggga
     4381 ggcgtgaaca acatcctgct gccccagggc aggccgccgg tgttccagca gcccgtcatc
     4441 tttctgggag cagacgtcac tcaccccccc gccggggatg ggaagaagcc ctccattgcc
     4501 gccgtggtgg gcagcatgga cgcccacccc aatcgctact gcgccaccgt gcgcgtgcag
     4561 cagcaccggc aggagatcat acaagacctg gccgccatgg tccgcgagct cctcatccag
     4621 ttctacaagt ccacgcgctt cgagcccacc cgcatcatct tctaccgcga cggtgtctct
     4681 gaaggccagt tccagcaggt tctccaccac gagttgctgg ccatccgtga ggcctgtatc
     4741 aagctagaaa aagactacca gcccgggatc accttcatcg tggtgcagaa gaggcaccac
     4801 acccggctct tctgcactga caagaacgag cgggttggga aaagtggaaa cattccagca
     4861 ggcacgactg tggacacgaa aatcacccac cccaccgagt tcgacttcta cctgtgtagt
     4921 cacgctggca tccaggggac aagcaggcct tcgcactatc acgtcctctg ggacgacaat
     4981 cgtttctcct ctgatgagct gcagatccta acctaccagc tgtgtcacac ctacgtgcgc
     5041 tgcacacgct ccgtgtccat cccagcgcca gcatactacg ctcacctggt ggccttccgg
     5101 gccaggtacc acctggtgga taaggaacat gacagtgctg aaggaagcca tacctctggg
     5161 cagagtaacg ggcgagacca ccaagcactg gccaaggcgg tccaggttca ccaagacact
     5221 ctgcgcacca tgtactttgc ttgaggcggc cgctcgagtc tagagggccc gcggttcgaa
     5281 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg tcatcatcac
     5341 catcaccatt gagtttaaac ccgctgatca gcctcgactg tgccttctaa ggcctgagct
     5401 cgctgatcag cctcgatcga ggatccagac atgataagat acattgatga gtttggacaa
     5461 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     5521 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     5581 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa
     5641 tgtggtatgg ctgattatga tcagtcgacc gatgcccttg agagccttca acccagtcag
     5701 ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat
     5761 catgcaactc gtaggacagg tgccggcagc gctctgggtc attttcggcg aggaccgctt
     5821 tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct tgcacgccct
     5881 cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc aggccattat
     5941 cgccggcatg gcggccgacg cgctgggcta cgtcttgctg gcgttcgcga cgcgaggctg
     6001 gatggccttc cccattatga ttcttctcgc ttccggcggc atcgggatgc ccgcgttgca
     6061 ggccatgctg tccaggcagg tagatgacga ccatcaggga cagcttcaag gccagcaaaa
     6121 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     6181 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     6241 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     6301 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     6361 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     6421 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     6481 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     6541 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     6601 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     6661 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     6721 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     6781 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     6841 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     6901 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     6961 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     7021 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     7081 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     7141 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     7201 taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt
     7261 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     7321 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     7381 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     7441 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     7501 gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag
     7561 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     7621 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     7681 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     7741 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     7801 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     7861 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     7921 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca
     7981 agaattaatt ctatattcta aaaacacaaa tgatacttct aaaaaaaaat
//
