LOCUS       sequence-355414-        9571 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsXRN1_1-1596.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9571)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 9571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pT7-EGFP-C1-HsXRN1_1-1596_O
FEATURES             Location/Qualifiers
     source          1..9571
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     613..632
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        613..631
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      659..668
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(665..667,668..670,671..1381)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(665..667,668..670,671..1381)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(710..731)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(971..990)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1318..1339
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6359..6480
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6396..6415)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6450..6469
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6487..6942)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6624..6645)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6836..6855
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6969..7073
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7075..7432
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7075..7266
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7098..7118)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7283..7418
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7467..8261
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7467..8261
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7521..7540)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8131..8150
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8449..8468)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8493..8540
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8869..9457
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9358..9377
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      601 gcttatcgaa attaatacga ctcactatag ggagacccaa gctctagcgc taccggtcgc
      661 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
      721 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      781 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      841 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
      901 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
      961 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     1021 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     1081 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     1141 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     1201 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     1261 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     1321 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     1381 gtccggactc agatctcgag ctcaagcttc gaattctgca gtcgacggta ccgcgggcat
     1441 gggagtcccc aagttttaca gatggatctc agagcggtat ccctgtctca gcgaagtggt
     1501 gaaagagcat cagattcctg aatttgacaa cttgtacctg gatatgaatg gaattataca
     1561 tcagtgctcc catcctaatg atgatgatgt tcactttaga atttcagatg ataaaatctt
     1621 tactgatatt tttcactacc tggaggtgtt gtttcgcatt attaaaccca ggaaagtgtt
     1681 ctttatggct gtagatggtg tggctcctcg agcaaaaatg aaccagcagc gtgggaggcg
     1741 ttttaggtca gcaaaggagg cagaagacaa aattaaaaag gcaatagaga agggagaaac
     1801 tcttcctaca gaggccagat ttgattccaa ctgtatcaca ccaggaactg aatttatggc
     1861 caggttacat gaacatctga agtattttgt aaatatgaaa atttccacag acaagtcatg
     1921 gcaaggagtt accatctact tctcaggcca tgagactcct ggagaaggag agcataaaat
     1981 catggaattt atcagatccg agaaagcaaa gccagatcat gatccaaaca ccagacactg
     2041 tctttatggt ttagatgctg acttgattat gcttggatta acaagtcatg aggcacattt
     2101 ttctctctta agagaagaag ttcgatttgg tggcaaaaaa acacaacggg tatgtgctcc
     2161 agaagaaact acatttcacc ttctacactt gtctttaatg agagagtata ttgactatga
     2221 gttttcagta ttaaaagaaa agatcacatt taaatatgat attgaaagga taatagatga
     2281 ttggattttg atggggtttc ttgttggtaa tgattttatc cctcatctac ctcatttaca
     2341 tattaatcat gatgcactgc ctcttcttta tggaacatat gttaccatcc tgccagaact
     2401 tgggggttat attaatgaaa gtgggcacct caacttacct cgatttgaga aataccttgt
     2461 gaaactatca gattttgatc gggagcactt cagtgaagtt tttgtggacc taaaatggtt
     2521 tgaaagcaaa gttggtaaca agtacctcaa tgaagcagca ggtgtcgcag cagaagaagc
     2581 caggaactac aaggaaaaga aaaagttaaa gggccaggaa aattctctgt gttggactgc
     2641 tttagacaaa aatgaaggcg aaatgataac ttctaaggat aatttagaag atgagactga
     2701 agatgatgac ctatttgaaa ctgagtttag acaatataaa agaacatatt acatgacgaa
     2761 gatgggggtt gacgtagtat ctgatgactt tctggctgat caagctgcat gttatgttca
     2821 ggcaatacag tggattttgc actattacta tcatggagtt cagtcctgga gctggtatta
     2881 tccttatcat tatgcacctt tcctgtctga tatacacaac atcagtacac tcaaaatcca
     2941 ttttgaacta ggaaaacctt ttaagccatt tgaacagctt cttgctgtac ttccagcagc
     3001 cagcaaaaat ttacttcctg catgctacca gcatttgatg accaatgaag actcaccaat
     3061 tatagaatat tacccacctg attttaaaac tgacctaaat gggaaacaac aggaatggga
     3121 agctgtggtg ttaatccctt ttattgatga gaagcgatta ttggaagcca tggagacatg
     3181 taaccactcc ctcaaaaagg aagagaggaa aagaaaccaa catagtgagt gcctaatgtg
     3241 ctggtatgat agagacacag agtttatcta tccttctcca tggccagaaa agttccctgc
     3301 catagaacga tgttgtacaa ggtataaaat aatatcctta gatgcttggc gtgtagacat
     3361 aaacaaaaac aaaataacca gaattgacca gaaagcatta tatttctgtg gatttcctac
     3421 tctgaaacac atcagacaca aatttttttt gaagaaaagt ggtgttcaag tattccagca
     3481 aagcagtcgt ggagaaaaca tgatgttgga aatcttagtg gatgcagaat cagatgaact
     3541 taccgtagaa aatgtagctt catcagtgct tggaaaatct gtctttgtta attggcctca
     3601 ccttgaggaa gctagagtcg tggctgtatc agatggagaa actaagtttt acttggaaga
     3661 acctccagga acacagaagc tttattcagg aagaactgcc ccaccatcta aagtggttca
     3721 tcttggagat aaagaacaat ctaactgggc aaaagaagta caaggaattt cagaacacta
     3781 cctgagaaga aaaggaataa taataaatga aacatctgca gttgtgtatg ctcagttact
     3841 cacaggtcgt aaatatcaaa taaatcaaaa tggtgaagtt cgtctagaga aacagtggtc
     3901 aaaacaagtt gttccttttg tttatcaaac tattgtcaag gacatccgag ctttcgactc
     3961 ccgtttctcc aatatcaaaa cattggatga tttgtttcct ctgagaagta tggtctttat
     4021 gctgggaact ccctattatg gctgcactgg agaagttcag gattcaggtg atgtgattac
     4081 agaaggtagg attcgtgtga ttttcagcat tccatgtgaa cccaatcttg atgctttaat
     4141 acagaaccag cataaatatt ctataaagta caacccagga tatgtgttgg ccagtcgcct
     4201 tggagtgagt ggataccttg tttcaaggtt tacaggaagt atttttattg gaagaggatc
     4261 taggagaaac cctcatggag accataaagc aaatgtgggt ttaaatctca aattcaacaa
     4321 gaaaaatgag gaggtacctg gatatactaa gaaagttgga agtgaatgga tgtattcatc
     4381 tgcagcagaa caacttctgg cagagtactt agagagagct ccagaactat ttagttatat
     4441 agccaaaaat agccaagagg atgtgttcta tgaagatgac atttggcctg gagaaaatga
     4501 gaatggtgct gaaaaagttc aagaaattat tacttggcta aaaggacatc ctgtcagtac
     4561 tttatctcgt tcttcttgtg atttacaaat tctggatgca gctattgttg agaaaattga
     4621 ggaagaagtc gaaaagtgca agcaaagaaa gaataataag aaggtgcgag taacagtgaa
     4681 accccatttg ctatacagac ctttagaaca gcaacatgga gtcattcctg atcgggatgc
     4741 agaattttgt ctttttgacc gtgttgtaaa tgtgagrgaa aacttctcag ttccagttgg
     4801 ccttcgaggc accatcatag gaataaaagg agctaataga gaagccgatg tactatttga
     4861 agtattattt gatgaagaat ttcctggagg gttaacaata agatgctcac ctggtagagg
     4921 ttatcgactg ccaacaagtg ccttggtgaa cctttctcat gggagtcgct ctgaaactgg
     4981 aaatcagaag ttgacagcca tcgtaaaacc acaaccagct gtacatcaac atagctcaag
     5041 ttcatcagtt tcctctgggc atttgggagc cctcaaccat tcccctcaat cactttttgt
     5101 tcctactcaa gtacctacta aagatgatga tgaattctgc aacatttggc agtccttaca
     5161 gggatctgga aagatgcaat actttcagcc aactatacaa gagaagggtg cagttctacc
     5221 tcaagaaata agccaagtaa atcaacatca taaatctggc tttaatgaca acagtgttaa
     5281 atatcagcaa agaaaacatg accctcacag aaaatttaaa gaagagtgta agagtcctaa
     5341 agctgagtgt tggtcccaaa aaatgtccaa taagcagcct aactctggaa ttgagaactt
     5401 tttagcatct ttgaatatct ccaaagaaaa tgaagtacag tcatctcatc atggggagcc
     5461 tccaagtgaa gagcatttgt caccacagtc atttgccatg ggaacacgga tgcttaaaga
     5521 aattctaaaa attgatggct ctaacactgt ggaccataag aatgaaatca aacagattgc
     5581 taatgaaatc cctgtttcct ctaacagaag agatgaatat ggattaccct ctcagcctaa
     5641 acaaaataag aaattagcat cttatatgaa caagcctcac agtgctaatg agtaccataa
     5701 tgttcagtct atggacaata tgtgttggcc tgcccccagc cagatccctc ctgtatccac
     5761 accagtaact gaactttctc gaatttgttc ccttgttgga atgccacaac ctgatttctc
     5821 ctttcttagg atgccacaga caatgaccgt ttgccaagta aaattatcta atggcttact
     5881 ggtacatggg ccacagtgcc actctgaaaa tgaagccaaa gagaaagctg cactttttgc
     5941 tttacaacag ttgggctcct taggcatgaa tttccctttg ccttcacaag tatttgcaaa
     6001 ttatccttca gctgtaccac ctggaaccat tcctccagcc tttcccccac ctactggctg
     6061 ggatcactat ggaagcaact atgcattggg ggcagctaat ataatgcctt cgtcgtctca
     6121 tctctttggc tcaatgccat ggggaccatc ggtgccagtt cctgggaagc ccttccatca
     6181 tactttatat tctgggacca tgcccatggc tgggggaata ccagggtaag gatccaccgg
     6241 atctagataa ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     6301 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     6361 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     6421 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     6481 acgcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     6541 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     6601 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     6661 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     6721 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     6781 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     6841 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     6901 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg
     6961 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     7021 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg
     7081 cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     7141 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     7201 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     7261 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     7321 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     7381 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc
     7441 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     7501 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     7561 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     7621 acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca
     7681 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     7741 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     7801 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     7861 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     7921 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     7981 ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     8041 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     8101 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     8161 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     8221 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
     8281 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     8341 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     8401 cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa
     8461 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca
     8521 cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga
     8581 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca
     8641 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc
     8701 catagcctca ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     8761 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     8821 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     8881 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     8941 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     9001 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     9061 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     9121 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     9181 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     9241 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     9301 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     9361 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     9421 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     9481 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     9541 ttctgtggat aaccgtatta ccgccatgca t
//
