LOCUS       sequence-355416-        9951 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual fluorescent reporter construct with no spacer, upstream TRE 
            (Tetracycline Response Element) recruitment site, arms for 
            integration at AAVS1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9951)
  AUTHORS   Lensch S, Herschl MH, Ludwig CH, Sinha J, Hinks MM, Mukund A, 
            Fujimori T, Bintu L
  TITLE     Dynamic spreading of chromatin-mediated gene silencing and 
            reactivation between neighboring genes in single cells.
  JOURNAL   Elife. 2022 Jun 9;11. pii: 75115. doi: 10.7554/eLife.75115.
  PUBMED    35678392
REFERENCE   2  (bases 1 to 9951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-06-9"; volume: "11. pii: 75115. doi"; pages: " 
            10.7554/eLife.75115"
COMMENT     Sequence Label: 
            pSL011_AAVS1-puro-9xTetO-pEF-H2B-mCitrine-pRSV-H2B-mCherry
FEATURES             Location/Qualifiers
     source          1..9951
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    7..32
                     /label=SA
                     /note="splice acceptor site"
     CDS             56..109
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             119..718
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(119..138)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     615..635
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(750..767)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    756..980
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    1053..1071
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1089..1107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1125..1143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1161..1179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1197..1215
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1233..1251
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1269..1287
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1305..1323
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1341..1359
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     intron          1604..2542
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2499..2519
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        2575..2593
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             2684..3061
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      3080..3089
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3086..3088,3089..3091,3092..3802)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3131..3152)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3392..3411)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3739..3760
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3853..3872)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3854..3872)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    4146..4267
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4312..4574
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     CDS             4588..4965
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      4978..4987
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4984..5697
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYKS"
     primer_bind     complement(5129..5147)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5397..5416
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    5813..5894
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    5989..6825
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     CDS             complement(join(7100..7891,7892..7960))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7723..7742
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8084..8672
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8573..8592
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8826..8843
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8960..8981
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8996..9001,9002..9019,9020..9026)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9034..9050
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9039..9061
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9058..9074
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9058..9074
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        9095..9113
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    9148..9951
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
ORIGIN
        1 aagcttctga cctcttctct tcctcccaca gggcctcgag agatctggca gcggagaggg
       61 cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggcccta ggctcgagat
      121 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
      181 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
      241 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
      301 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
      361 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
      421 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
      481 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
      541 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
      601 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
      661 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgatc
      721 tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
      781 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
      841 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
      901 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
      961 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctgggggc tagcaaagcc
     1021 taggaacagc tgcatatagc actgcagttt actccctatc agtgatagag agtcaaaagg
     1081 ccatgccctc cctatcagtg atagagagag cagactcaag gtggtcccta tcagtgatag
     1141 agagattgag caagagggcc tccctatcag tgatagagaa tagcactgca gtttactccc
     1201 tatcagtgat agagagtcaa aaggccatgc cctccctatc agtgatagag agagcagact
     1261 caaggtggtc cctatcagtg atagagagat tgagcaagag ggcctcccta tcagtgatag
     1321 agattgatcc agtcagggcc tccctatcag tgatagagaa gctatgtgca ggtagggctg
     1381 tacagtggga atgcatgagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
     1441 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
     1501 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
     1561 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
     1621 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
     1681 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
     1741 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg
     1801 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa
     1861 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag
     1921 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
     1981 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
     2041 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
     2101 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
     2161 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
     2221 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
     2281 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc
     2341 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
     2401 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt
     2461 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca
     2521 aagttttttt cttccatttc aggtgtcgtg agctagcgat tacgccaagc tcgaaattaa
     2581 ccctcactaa agggaacaaa agctggagct ccaccgcggt ggcggccgct ctagcccggg
     2641 cggatccccc gggctgcagg aattcgatat caagcttgcc accatgccag agccagcgaa
     2701 gtctgctccc gccccgaaaa agggctccaa gaaggcggtg actaaggcgc agaagaaagg
     2761 cggcaagaag cgcaagcgca gccgcaagga gagctattcc atctatgtgt acaaggttct
     2821 gaagcaggtc caccctgaca ccggcatttc gtccaaggcc atgggcatca tgaattcgtt
     2881 tgtgaacgac attttcgagc gcatcgcagg tgaggcttcc cgcctggcgc attacaacaa
     2941 gcgctcgacc atcacctcca gggagatcca gacggccgtg cgcctgctgc tgcctgggga
     3001 gttggccaag cacgccgtgt ccgagggtac taaggccatc accaagtaca ccagcgctaa
     3061 ggatccccgg gtaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt
     3121 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     3181 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     3241 caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgatgtgctt
     3301 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     3361 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     3421 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     3481 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     3541 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     3601 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     3661 ccccgtgctg ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc
     3721 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     3781 cggcatggac gagctgtaca agtaggtcga cctcgagggg gggcccggta ccaggtaagt
     3841 gtacccaatt cgccctatag tgagtcgtat tacaattcac tcgatcgccc ttcccaacag
     3901 ttgcgcagcc tgaatggcga atggagatcc aatttttaag tgtataatgt gttaaactac
     3961 tgattctaat tgtttgtgta ttttagattc acagtcccaa ggctcatttc aggcccctca
     4021 gtcctcacag tctgttcatg atcataatca gccataccac atttgtagag gttttacttg
     4081 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     4141 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     4201 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     4261 tatcttaacg cgtaaattgt aagcgtctcg gcctctagag acgtccatat gaatgtagtc
     4321 ttatgcaata cacttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
     4381 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
     4441 attaggaagg caacagacag gtctgacatg gattggacga accactgaat tgcgcattgc
     4501 agagataatt gtatttaagt gcctagctcg atacaataaa cgccatttga ccattcacca
     4561 cattggtgtg caccctgcag atctagaatg ccagagccag cgaagtctgc tcccgccccg
     4621 aaaaagggct ccaagaaggc ggtgactaag gcgcagaaga aaggcggcaa gaagcgcaag
     4681 cgcagccgca aggagagcta ttccatctat gtgtacaagg ttctgaagca ggtccaccct
     4741 gacaccggca tttcgtccaa ggccatgggc atcatgaatt cgtttgtgaa cgacattttc
     4801 gagcgcatcg caggtgaggc ttcccgcctg gcgcattaca acaagcgctc gaccatcacc
     4861 tccagggaga tccagacggc cgtgcgcctg ctgctgcctg gggagttggc caagcacgcc
     4921 gtgtccgagg gtactaaggc catcaccaag tacaccagcg ctaaggatcc accggtcgcc
     4981 accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
     5041 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
     5101 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
     5161 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
     5221 caccccgccg acattcccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
     5281 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
     5341 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
     5401 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
     5461 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
     5521 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
     5581 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     5641 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtcttaagcg
     5701 actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     5761 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     5821 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     5881 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat ctcatatgta
     5941 aattgtaagc gtctcggcct ctagagtcga gatgacattg attattgaac tagggacagg
     6001 attggtgaca gaaaagcccc atccttaggc ctcctccttc ctagtctcct gatattgggt
     6061 ctaaccccca cctcctgtta ggcagattcc ttatctggtg acacaccccc atttcctgga
     6121 gccatctctc tccttgccag aacctctaag gtttgcttac gatggagcca gagaggatcc
     6181 tgggagggag agcttggcag ggggtgggag ggaagggggg gatgcgtgac ctgcccggtt
     6241 ctcagtggcc accctgcgct accctctccc agaacctgag ctgctctgac gcggctgtct
     6301 ggtgcgtttc actgatcctg gtgctgcagc ttccttacac ttcccaagag gagaagcagt
     6361 ttggaaaaac aaaatcagaa taagttggtc ctgagttcta actttggctc ttcacctttc
     6421 tagtccccaa tttatattgt tcctccgtgc gtcagtttta cctgtgagat aaggccagta
     6481 gccagccccg tcctggcagg gctgtggtga ggaggggggt gtccgtgtgg aaaactccct
     6541 ttgtgagaat ggtgcgtcct aggtgttcac caggtcgtgg ccgcctctac tccctttctc
     6601 tttctccatc cttctttcct taaagagtcc ccagtgctat ctgggacata ttcctccgcc
     6661 cagagcaggg tcccgcttcc ctaaggccct gctctgggct tctgggtttg agtccttggc
     6721 aagcccagga gaggcgctca ggcttccctg tcccccttcc tcgtccacca tctcatgccc
     6781 ctggctctcc tgccccttcc ctacaggggt tcctggctct gctcttcgcc ttcttgacga
     6841 gttcttctga attattaacg cttacaattt cctgatgcgg tattttctcc ttacgcatct
     6901 gtgcggtatt tcacaccgca tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     6961 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagatta tcaaaaagga
     7021 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     7081 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     7141 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     7201 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     7261 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     7321 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     7381 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     7441 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     7501 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     7561 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     7621 catccgtaag atgcttttct gtgactggtg aatactcaac caagtcattc tgagaatagt
     7681 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     7741 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     7801 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     7861 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     7921 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     7981 attgaagcat ttatcagggt tattgtctca tgaccaaaat cccttaacgt gagttttcgt
     8041 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     8101 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     8161 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     8221 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     8281 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     8341 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     8401 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     8461 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     8521 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     8581 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     8641 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     8701 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     8761 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     8821 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     8881 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     8941 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     9001 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     9061 gaaacagcta tgaccatgat tacgccaagc tcagaattaa ccctcactaa agggactagt
     9121 cctgcaggtt taaacgaatt cgccctttgc tttctctgac cagcattctc tcccctgggc
     9181 ctgtgccgct ttctgtctgc agcttgtggc ctgggtcacc tctacggctg gcccagatcc
     9241 ttccctgccg cctccttcag gttccgtctt cctccactcc ctcttcccct tgctctctgc
     9301 tgtgttgctg cccaaggatg ctctttccgg agcacttcct tctcggcgct gcaccacgtg
     9361 atgtcctctg agcggatcct ccccgtgtct gggtcctctc cgggcatctc tcctccctca
     9421 cccaacccca tgccgtcttc actcgctggg ttcccttttc cttctccttc tggggcctgt
     9481 gccatctctc gtttcttagg atggccttct ccgacggatg tctcccttgc gtcccgcctc
     9541 cccttcttgt aggcctgcat catcaccgtt tttctggaca accccaaagt accccgtctc
     9601 cctggcttta gccacctctc catcctcttg ctttctttgc ctggacaccc cgttctcctg
     9661 tggattcggg tcacctctca ctcctttcat ttgggcagct cccctacccc ccttacctct
     9721 ctagtctgtg ctagctcttc cagccccctg tcatggcatc ttccaggggt ccgagagctc
     9781 agctagtctt cttcctccaa cccgggcccc tatgtccact tcaggacagc atgtttgctg
     9841 cctccaggga tcctgtgtcc ccgagctggg accaccttat attcccaggg ccggttaatg
     9901 tggctctggt tctgggtact tttatctgtc ccctccaccc cacagtgggg c
//
