LOCUS       sequence-355423-        7392 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmMextli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7392)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 7392)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmMextli_AA
FEATURES             Location/Qualifiers
     source          1..7392
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        12..2521
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2541..2606
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2631..2657
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4654..4695
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4705..4722
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4745..4762)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4795..4929
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4819..4838)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4873..4892
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(5530..6118)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5610..5629)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6289..7080,7081..7149))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6912..6931
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7150..7254)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7322..7340
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 taaaaaaaaa tcatgaatgg catcaactct gaatcaaatc tttgcagatg cacctacttc
       61 tcatttccac tgtcacatca tttttccaga tctcgctgcc tgttatgtgg cccacaaacc
      121 aagacacgtt ttatggccat taaagctggc tgatcgtcgc caaacaccaa atacataatg
      181 aatatgtaca cattcgagaa agaagcgatc aaagaagcgt cttcgggcgg agtaggagaa
      241 tgcggaggag aaggagaacg agctgatcta gtatctctcc acaatccaat gccaactgac
      301 caactggcca tattcggagc aatttgaagc caatttccat cgcctggcga tcgctccatt
      361 cttggctata tgtttttcac cgttacccgg ggccattttc aaagactcgt cggcaagata
      421 agattgtgtc actcgctgtc tctcttcatt tgtcgaagaa tgctgaggaa tttcgcgatg
      481 acgtcggcga gtattttgaa gaatgagaat aatttgtatt tatacgaaaa tcagttagtg
      541 gaattttcta caaaaacatg ttatctatag ataattttgt tgcaaaatat gttgactatg
      601 acaaagattg tatgtatata cctttaatgt attctcattt tcttatgtat ttataatggc
      661 aatgatgata ctgatgatat tttaagatga tgccagacca aaaggcttga atttctgcgt
      721 cttttgccga acgcagtgca tgtgcaattg ttgttttttg gaatattcaa ttttcggact
      781 gtccgctttg atttcagttt cttggcttat tcaaaaagca aagtaaagcc aaaaaagcga
      841 gatggcaata ccaaatgcgg caaaacggta gtggaaggaa aggggtgcgg ggcagcggaa
      901 ggaagggtgg ggcggggcgt ggcggggtct gtggctgggc gcgacgtcac cgacgttgga
      961 gccactcctt tgaccatgtg tgcgtgtgtg tattattcgt gtctcgccac tcgccggttg
     1021 tttttttctt tttatgctgc gctctctcta gcgccatctc gcttacgcat gctcaacgca
     1081 ccgcatgttg ccgtttcctt ttatgcgtca ttttggctcg aaataggcaa ttatttaaac
     1141 aaagattagt caacgaaaac gctaaaataa ataagtctac aatatggtta cttattgcca
     1201 tgtgtgtgca gccaacgata gcaacaaaag caacaacaca ggtggctttc cctctttcac
     1261 tttttgtttg caagccgcgt gcgagcaaga cggcacgacc ggcaaacgca attacgctga
     1321 caaagagcag acgaagtttt ggcgaaaaac atcaaggcgc ctgatacgaa tgcatttgca
     1381 ataacaattg cgatatttaa tattgtttat gaagctgttt gacttcaaaa cacacaaaaa
     1441 aaaaaataaa acaaattatt tgaaagagaa ttaggaatcg gacgcttatc gttagggtaa
     1501 caacaagaaa tgcttactga gtcacagcct ctggaaaact gccgcaagcc agagagagag
     1561 agaaaaagag ggagagcagc ttagaccgca tgtgcttgtg tgtgaggcgt ctctctcttc
     1621 gtctctgttg cgcaaacgca tagactgcac tgaaaaaatc gattacctat tttttatgaa
     1681 tgaatatttg cactattact attcaaaact attaagatag caatcacatt caatagccaa
     1741 atactatacc acctgagcga tgcaacgaaa tgatcaattt gagcaaaaat gctgcatatt
     1801 taggacggca tcattataga aatgcttctt gctgtgtact tttctctcgt ctggcagctg
     1861 tttcgccgtt attgttaaaa ccggcttaag ttaggtgtgt tttctacgac tagtgaatgc
     1921 cctactagaa gatgtgtgtt gcacaaaatg tccctggaat aaccaatttg aagtgcagat
     1981 agcagtaaac gtaagctaat atgaatatta tttaactgta atgttttaat atcgctggac
     2041 attactaata aacccactat aaacacatgt acatatgtat gttttggcat acaatgagta
     2101 gttggggaaa aaatgtgtaa aagcaccgtg accatcacag cataaagata accagctgaa
     2161 gtatcgaata tgagtaaccc ccaaattgaa tcacatgccg caactgatag gacccatgga
     2221 agtacactct tcatggcgat atacaagaca cacacaagca cgaacaccca gttgcggagg
     2281 aaattctccg taaatgaaaa cccaatcggc gaacaattca tacccatata tggtaaaagt
     2341 tttgaacgcg acttgagagc ggagagcatt gcggctgata aggttttagc gctaagcggg
     2401 ctttataaaa cgggctgcgg gaccagtttt catatcacta ccgtttgagt tcttgtgctg
     2461 tgtggatact cctcccgaca caaagccgct ccatcagcca gcagtcgtct aatccagaga
     2521 ccccggatcg gggtaccaac atggacgcac aaacacgacg acgtgagcgt cgcgctgaga
     2581 aacaagctca atggaaagct gcaaacggtg gatctcctcc acatatggct tacccatacg
     2641 atgttccaga ttacgctcct ccaagcttga attctatggc tcacacacac ctgggacgcg
     2701 ccgtgaaaaa cattgaggcg ccacgcccgt tgaagacaca atcgcgatcg tcgttgaaga
     2761 acagctacct ggtgatcgag gagctaatcc agttgataga caacgtgacc gtcggtcttc
     2821 agtcgtgcaa caccacgccg gaatcgatca cgctgctcct gcacaacctc cgggtgcatg
     2881 gaccgcaact ggaggcggtg tccaaggata cactggaccg ggccttcgtc gttttccgca
     2941 acgcttcgca ggacgaacgg ctgaatataa cgacgcgcct caagctgctc gaactcatcg
     3001 agctgcgcgc caagagctgg gacaatgacg acacaatcgc gtactacaag tccaagcagc
     3061 agatctccaa cgtggagctg ccttcggaat accagtacga tgccggcgtg cagcctggcg
     3121 ccttcagcac ctcccccaca ttcggcgtca gcggcggtgt tggtggcgtt aacgatggag
     3181 ccgcagccgc cgctgccgcc gtcttcaatg cagcatctgc agctgcagcc gcccaagctg
     3241 cggccattgc ggccgtgggc acatccaacc agcagcacat gctgctgccg cccggtgagg
     3301 tcattcgtaa ttcgggcaag ttccccaaac cgaccaagat tcctggcaag acgtactgca
     3361 aggacgaggt cgtgattcgc aatgctgact ccggcaaagt aatgggcatc aaggggcgtc
     3421 gcgtgcacat gatcgaggag ctaagcgaaa ccattatatc gtttcaaaga gtcaacccgg
     3481 gtgccaagga gcgtttggtg cagattactg gtccagctga ggataaaatt aactatgcca
     3541 aacaactgat ggaggacacc attcgtcgga acgcctcgcc ggtgcgcctg gaacccgctc
     3601 ctgccgttgg aggatcctgc tcctcgctta actcgtccaa ctcggacgac gccattgtgc
     3661 aaccaagaac gccaaccgga agcagtttgg ccaaccggct aagcttcaac tcggcccaaa
     3721 acttcatgac cgccaccgct gctgcacagc agatctcgca gcagatgcac caccaaacgc
     3781 atcacctgca gcaccaacag cagcagcagg tcgccgcagt agccgccgca gcagcagcgg
     3841 cggcacatgc tcaggcgaca gccgctgccg gtaaagtctt gcgccccaat caacagctgc
     3901 taatgcactc atattccaca aacgatgctt ccgtaggtga atacaagttc acggtcaacg
     3961 tgggccagca cctgatcaag atcaccggcg actgctgcga actagtgcgc gtggccaaac
     4021 tcgtgctcga tgactatttt agcagctcgg agtttctggc atccattgag gctggcgccg
     4081 ccttcgacgg cacttcgctg gtgaccacgc catcgactcc gcttcccgga gccgggccgc
     4141 cgcaattttg gctgcccggc acggacagtg gcattggact gaattgcgtt gtttcctcgt
     4201 cagcgaacaa caatggtgaa ggcgacgacg aggtgttcgc tgagcccagc aatggaggat
     4261 catcgactag caatcagaat ggactggcca gatcgcgtcg cagccacttt tcgcgcaagg
     4321 agtccacgcc ggagaccaag ggcgctcggg aaaagggtga cttggacgat ttggcaggca
     4381 ccaattcctt gaaaagcaat gcatctcgcg tttcttacga tattgaacac ttgctctact
     4441 actccatgag tccgcactcg tggaccctgc ccaccgactg gcaaaagatg caggagacag
     4501 cgccctcgat tctgcgcaac aaggatctgc aggatgagag ccagcgcttc gatggtgata
     4561 agtatctggc cagcattaag acggccgcca agcgagacat tgtggctgcc gatgaggtcg
     4621 agactctgga cgaataaggg cccgcggttc gaaggtaagc ctatccctaa ccctctcctc
     4681 ggtctcgatt ctacgcgtac cggtcatcat caccatcacc attgagttta aacccgctga
     4741 tcagcctcga ctgtgccttc taaggcctga gctcgctgat cagcctcgat cgaggatcca
     4801 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     4861 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     4921 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     4981 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta tgatcagtcg
     5041 accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
     5101 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc
     5161 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct
     5221 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc
     5281 caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg
     5341 ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct
     5401 cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga
     5461 cgaccatcag ggacagcttc aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     5521 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     5581 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     5641 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     5701 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     5761 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     5821 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     5881 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     5941 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     6001 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     6061 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     6121 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     6181 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     6241 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     6301 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     6361 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     6421 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     6481 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     6541 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     6601 tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     6661 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     6721 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     6781 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     6841 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     6901 gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     6961 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     7021 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     7081 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     7141 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     7201 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     7261 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     7321 aaataggcgt atcacgaggc cctttcgtct tcaagaatta attctatatt ctaaaaacac
     7381 aaatgatact tc
//
