LOCUS       sequence-355427-        2602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-CT3
FEATURES             Location/Qualifiers
     source          1..2602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(89..108)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(90..108)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(113..129)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(113..129)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      536..1124
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1025..1044
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1251..1256,1257..1273,1274..1279)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             1327..2517
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1679..1698)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 aaccaaccct tgatcgggga agaacagtat gtcgagctat tttttgactt actggggatc
       61 aagcctgatt gggagaaaat aaaatatccc ctatagtgag tcgtattaca tggtcatagc
      121 tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa
      181 aaatatatca tcatgcctcc tctagaccaa agctggtctc actacaactt gctcgggcgt
      241 caggccgtgg gcttgacaga gaactggtag aaggcgctga acagtctcaa gagcctgttt
      301 gccaccccca ttactagcaa tcgctacaac ttgcgctggg gttagtccgt gggcttgaca
      361 gagaactggt agaaggcgtt ggacggtttc aagagcctgt ttgccaccgt catgactagc
      421 aatcgctacc acgagaccat atctggtcga gccacccatg accaaaatcc cttaacgtga
      481 gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
      541 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
      601 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
      661 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
      721 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
      781 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
      841 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
      901 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
      961 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     1021 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     1081 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     1141 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     1201 ccacagaatc aggggataac gcaggaaaga acatgaatta attctcatgt ttgacagctt
     1261 atcatcgatt agctttaatg cggtagttta tcacagttaa attgctaacg cagtcaggca
     1321 ccgtgtatga aatctaacaa tgcgctcatc gtcatcctcg gcaccgtcac cctggatgct
     1381 gtaggcatag gcttggttat gccggtactg ccgggcctct tgcgggatat cgtccattcc
     1441 gacagcatcg ccagtcacta tggcgtgctg ctagcgctat atgcgttgat gcaatttcta
     1501 tgcgcacccg ttctcggagc actgtccgac cgctttggcc gccgcccagt cctgctcgct
     1561 tcgctacttg gagccactat cgactacgcg atcatggcga ccacacccgt cctgtggatt
     1621 ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc
     1681 tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct
     1741 tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc
     1801 ttacatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc
     1861 ttcctaatgc aggagtcgca taagggagag cgccgaccca tgcccttgag agccttcaac
     1921 ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc
     1981 ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat tttcggcgag
     2041 gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg
     2101 cacgccctcg ctcaagcctt cgtcactggt cccgccacca aacgtttcgg cgagaagcag
     2161 gccattatcg ccggcatggc ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg
     2221 cgaggctgga tggccttccc cattatgatt cttctcgctt ccggcggcat cgggatgccc
     2281 gcgttgcagg ccatgctgtc caggcaggta gatgacgacc atcagggaca gcttcaagga
     2341 tcgctcgcgg ctcttaccag cctaacttcg atcattggac cgctgatcgt cacggcgatt
     2401 tatgccgcct cggcgagcac atggaacggg ttggcatgga ttgtaggcgc cgccctatac
     2461 cttgtctgcc tccccgcgtt gcgtcgcggt gcatggagcc gggccacctc gacctgaatg
     2521 gaagccggcg gcacctcgct aacggattca ccactccaag aattggagcc aatcaattct
     2581 tgcggagaac tgtgaatgcg ca
//
