LOCUS       sequence-35543-e         614 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller 
            JA, Somia NV, Bogdanove AJ, Voytas DF
  TITLE     Efficient design and assembly of custom TALEN and other TAL 
            effector-based constructs for DNA targeting.
  JOURNAL   Nucleic Acids Res. 2011 Jul;39(12):e82. doi: 10.1093/nar/gkr218
  PUBMED    21493687
REFERENCE   2  (bases 1 to 614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-07"; volume: "39(12):e82. doi"; pages: " 
            10.1093/nar/gkr21"
COMMENT     Sequence Label: pTAL4
FEATURES             Location/Qualifiers
     source          1..614
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             62..82
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             83..109
                     /codon_start=1
                     /product="epitope tag from the baculovirus Autographa 
                     californica GP64 envelope fusion protein"
                     /label=AcV5 tag
                     /translation="SWKDASGWS"
ORIGIN
        1 actcgagaaa gatattgtat atatcgtaac aataggaggt tcaacaatgg cttcctcccc
       61 tccaaagaaa aagagaaagg ttagttggaa ggacgcaagt ggttggtcta gaatgcatgc
      121 ggatcccatt cgtccgcgca ggccaagtcc tgcccgcgag cttctgcccg gaccccaacc
      181 ggatagggtt cagccgactg cagatcgtgg ggtgtctgcg cctgctggca gccctctgga
      241 tggcttgccc gctcggcgga cggtgtcccg gacccggctg ccatctcccc ctgcgccctc
      301 acctgcgttc tcggcgggca gcttcagcga tctgctccgt ccgttcgatc cgtcgcttct
      361 tgatacatcg cttcttgatt cgatgcctgc cgtcggcacg ccgcatacag cggctgcccc
      421 agcagagtgg gatgaggcgc aatcggctct gcgtgcagcc gatgacccgc cacccaccgt
      481 gcgtgtcgct gtcactgccg cgcggccgcc gcgcgccaag ccggccccgc gacggcgtgc
      541 tgcgcaaccc tccgacgctt cgccggccgc gcaggtggat ctacgcacgc tcggctacag
      601 tcagcagcag caag
//
