LOCUS       sequence-35544-e         638 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller 
            JA, Somia NV, Bogdanove AJ, Voytas DF
  TITLE     Efficient design and assembly of custom TALEN and other TAL 
            effector-based constructs for DNA targeting.
  JOURNAL   Nucleic Acids Res. 2011 Jul;39(12):e82. doi: 10.1093/nar/gkr218
  PUBMED    21493687
REFERENCE   2  (bases 1 to 638)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-07"; volume: "39(12):e82. doi"; pages: " 
            10.1093/nar/gkr21"
COMMENT     Sequence Label: pTAL4
FEATURES             Location/Qualifiers
     source          1..638
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    27..43
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     32..54
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     51..67
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     51..67
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     86..106
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        88..106
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(153..172)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(154..172)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(182..199)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(182..198)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(191..213)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
       61 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
      121 ggacctcgag ggggggcccg gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
      181 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
      241 gccttgcagc acatccccct ttcgccagct ggcgtaatan cgaagaggcc cgcaccgatc
      301 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgccc
      361 gtctccaggg gggcacccgt cagtgcattg cgcgatgcat gcactgcctc cattgcggtc
      421 acgccgccac gttttgcaat cttcacaagt tggcctgtgt ccaactgtaa cggcggacct
      481 ctcaactccc ccgcatccgt gagcaaggcc tccagggcgc gtgcgccgga ccactgtttg
      541 ccgacgccaa cgatgtcttc gtgtgtcgcc tctggcaacg ccgtgattat gtgctgatac
      601 gtgacagcga cggtccctaa cgctgccggg tgttggct
//
