LOCUS       sequence-355445-        2600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-CT8
FEATURES             Location/Qualifiers
     source          1..2600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     376..395
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(602..607,608..624,625..630)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             678..1868
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1030..1049)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2042..2061)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2043..2061)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2066..2082)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2066..2082)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      join(2487..2600,1..475)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
       61 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
      121 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
      181 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
      241 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
      301 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
      361 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
      421 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
      481 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
      541 tcctgcgtta tccacagaat caggggataa cgcaggaaag aacatgaatt aattctcatg
      601 tttgacagct tatcatcgat tagctttaat gcggtagttt atcacagtta aattgctaac
      661 gcagtcaggc accgtgtatg aaatctaaca atgcgctcat cgtcatcctc ggcaccgtca
      721 ccctggatgc tgtaggcata ggcttggtta tgccggtact gccgggcctc ttgcgggata
      781 tcgtccattc cgacagcatc gccagtcact atggcgtgct gctagcgcta tatgcgttga
      841 tgcaatttct atgcgcaccc gttctcggag cactgtccga ccgctttggc cgccgcccag
      901 tcctgctcgc ttcgctactt ggagccacta tcgactacgc gatcatggcg accacacccg
      961 tcctgtggat tctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg
     1021 ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc
     1081 tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg
     1141 gcgccatctc cttacatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac
     1201 tactgggctg cttcctaatg caggagtcgc ataagggaga gcgccgaccc atgcccttga
     1261 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac
     1321 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca
     1381 ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat
     1441 tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg
     1501 gcgagaagca ggccattatc gccggcatgg cggccgacgc gctgggctac gtcttgctgg
     1561 cgttcgcgac gcgaggctgg atggccttcc ccattatgat tcttctcgct tccggcggca
     1621 tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt agatgacgac catcagggac
     1681 agcttcaagg atcgctcgcg gctcttacca gcctaacttc gatcattgga ccgctgatcg
     1741 tcacggcgat ttatgccgcc tcggcgagca catggaacgg gttggcatgg attgtaggcg
     1801 ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct
     1861 cgacctgaat ggaagccggc ggcacctcgc taacggattc accactccaa gaattggagc
     1921 caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac ccttgatcgg ggaagaacag
     1981 tatgtcgagc tattttttga cttactgggg atcaagcctg attgggagaa aataaaatat
     2041 cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc
     2101 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgcc tcctctagac
     2161 caaagctggt ctcgaagtca tgacggtggc aaacaggctc ttgaaaccgt ccaacgcctt
     2221 ctaccagttc tctgtcaagc ccacggacta accccagcgc aagttgtagc gattgctagt
     2281 aatgggggtg gcaaacaggc tcttgagact gttcagcgcc ttctaccagt tctctgtcaa
     2341 gcccacggcc tgacgcccga gcaagttgta gcgattgctt cctgagacca tatctggtcg
     2401 agccacccat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac
     2461 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     2521 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     2581 actctttttc cgaaggtaac
//
