LOCUS       sequence-35545-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller 
            JA, Somia NV, Bogdanove AJ, Voytas DF
  TITLE     Efficient design and assembly of custom TALEN and other TAL 
            effector-based constructs for DNA targeting.
  JOURNAL   Nucleic Acids Res. 2011 Jul;39(12):e82. doi: 10.1093/nar/gkr218
  PUBMED    21493687
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-07"; volume: "39(12):e82. doi"; pages: " 
            10.1093/nar/gkr21"
COMMENT     Sequence Label: pTAL4
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..427)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGARLQGG
                     TRQCIARCMHCLHCGHAATFCNLHKLACVQL"
     primer_bind     277..299
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     291..308
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     292..308
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     318..337
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        318..336
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(384..404)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(384..402)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(423..439)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(423..439)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(436..458)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    447..463
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(471..477,478..495,496..501))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    516..537
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ggagttgaga ggtccgccgt tacagttgga cacaggccaa cttgtgaaga ttgcaaaacg
       61 tggcggcgtg accgcaatgg aggcagtgca tgcatcgcgc aatgcactga cgggtgcccc
      121 cctggagacg ggcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
      241 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
      301 cggccagtga gcgcgcgtaa tacgactcac tatagggcga attgggtacc gggccccccc
      361 tcgaggtcct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca
      421 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
      481 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
      541 gcgttgcgct cactgcccgc tttccaccgg tcgtctccaa cgaccacctc gtcgccttgg
      601 cctgcctcgg cggacgtcct gccatggatg cagtgaaaaa gggattgccg cacgcgccgg
      661 aattgatcag aagagtcaat cgccgtattg gcgaacgcac gtcccatcgc gttgccgact
      721 acgcgcaagt ggttcgcgtg ctggagtttt tccagtgcca ctcccaccca gcgtacgcat
      781 ttgatgaggc catgacgcag ttcgggatga gcaggaacgg gttggtacag ctctttcgca
      841 gagtgggcgt caccgaactc gaagcccgcg gtggaacgct ccccccag
//
