LOCUS       sequence-355454-       11841 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses rTetR-HDAC4(human) for gene silencing, integration by 
            Piggybac.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11841)
  AUTHORS   Lensch S, Herschl MH, Ludwig CH, Sinha J, Hinks MM, Mukund A, 
            Fujimori T, Bintu L
  TITLE     Dynamic spreading of chromatin-mediated gene silencing and 
            reactivation between neighboring genes in single cells.
  JOURNAL   Elife. 2022 Jun 9;11. pii: 75115. doi: 10.7554/eLife.75115.
  PUBMED    35678392
REFERENCE   2  (bases 1 to 11841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-06-9"; volume: "11. pii: 75115. doi"; pages: " 
            10.7554/eLife.75115"
COMMENT     Sequence Label: pLB37_PB_pGK-H2B-mIFP-T2A-rTetR-HDAC4-zeo
FEATURES             Location/Qualifiers
     source          1..11841
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     84..103
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(322..426)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(452..907)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(589..610)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     801..820
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1034..1056
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1048..1065
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1049..1065
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   1195..1257
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        1693..2192
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1728..1751)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2125..2144
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2222..2599
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             2618..3577
                     /codon_start=1
                     /product="naturally monomeric infrared fluorescent protein 
                     derived from a truncated bacteriophytochrome (Yu et al., 
                     2015)"
                     /label=mIFP
                     /translation="MSVPLTTSAFGHAFLANCEREQIHLAGSIQPHGILLAVKEPDNVV
                     IQASINAAEFLNTNSVVGRPLRDLGGDLPLQILPHLNGPLHLAPMTLRCTVGSPPRRVD
                     CTIHRPSNGGLIVELEPATKTTNIAPALDGAFHRITSSSSLMGLCDETATIIREITGYD
                     RVMVVRFDEEGNGEILSERRRADLEAFLGNRYPASTIPQIARRLYEHNRVRLLVDVNYT
                     PVPLQPRISPLNGRDLDMSLSCLRSMSPIHQKYMQDMGVGATLVCSLMVSGRLWGLIAC
                     HHYEPRFVPFHIRAAGEALAETCAIRIATLESFAQSQSK"
     CDS             3578..3631
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(7542..7559)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7548..7755
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7788..7808)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7793..8122
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7973..8108
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8035..8054
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8170..8217
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8236..8610
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     primer_bind     complement(8670..8690)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    8732..8853
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8769..8788)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8823..8842
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   9616..9650
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(10141..10157)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10141..10157)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10154..10176)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10165..10181
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10189..10195,10196..10213,10214..10219))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10234..10255
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10372..10389)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10543..11131)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10623..10642)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11302..11841,1..252,253..321))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
       61 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
      121 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
      181 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
      241 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      301 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
      361 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
      421 tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa
      481 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
      541 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
      601 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
      661 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
      721 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
      781 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
      841 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
      901 ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
      961 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     1021 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcctcgttc
     1081 attcacgttt ttgaacccgt ggaggacggg cagactcgcg gtgcaaatgt gttttacagc
     1141 gtgatggagc agatgaagat gctcgacacg ctgcagaaca cgcagctaga ttaaccctag
     1201 aaagataatc atattgtgac gtacgttaaa gataatcatg tgtaaaattg acgcatgtgt
     1261 tttatcggtc tgtatatcga ggtttattta ttaatttgaa tagatattaa gttttattat
     1321 atttacactt acatactaat aataaattca acaaacaatt tatttatgtt tatttattta
     1381 ttaaaaaaaa caaaaactca aaatttcttc tataaagtaa caaaactttt atgagggaca
     1441 gccccccccc aaagccccca gggatgtaat tacgtccctc ccccgctagg gggcagcagc
     1501 gagccgcccg gggctccgct ccggtccggc gctccccccg catccccgag ccggcagcgt
     1561 gcggggacag cccgggcacg gggaaggtgg cacgggatcg ctttcctctg aacgcttctc
     1621 gctgctcttt gagcctgcag acacctgggg ggatacgggg aaaaggcctc cacggccact
     1681 agtaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag
     1741 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
     1801 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc
     1861 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa
     1921 tggaagtagc acgtctcact aggctcgtgc agatggacag caccgctgag caatggaagc
     1981 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga
     2041 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg
     2101 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc
     2161 tgttctcctc ttcctcatct ccgggccttt cgacctgcag cccaagcttg ctagcgccac
     2221 catgccagag ccagcgaagt ctgctcccgc cccgaaaaag ggctccaaga aggcggtgac
     2281 taaggcgcag aagaaaggcg gcaagaagcg caagcgcagc cgcaaggaga gctattccat
     2341 ctatgtgtac aaggttctga agcaggtcca ccctgacacc ggcatttcgt ccaaggccat
     2401 gggcatcatg aattcgtttg tgaacgacat tttcgagcgc atcgcaggtg aggcttcccg
     2461 cctggcgcat tacaacaagc gctcgaccat cacctccagg gagatccaga cggccgtgcg
     2521 cctgctgctg cctggggagt tggccaagca cgccgtgtcc gagggtacta aggccatcac
     2581 caagtacacc agcgctaagg atccaccggt cgccaccatg tcggtaccgc tgactacctc
     2641 agcattcggc cacgcgtttc tggctaactg tgaacgcgag cagatccacc tggcgggctc
     2701 cattcagccg cacggtatcc tgctggctgt gaaagagccg gacaacgtgg tgatccaggc
     2761 ttctattaac gctgcggagt tcctgaacac caactctgtt gttggccgtc cgctgcgtga
     2821 cctgggcggc gatctgcctt tgcagatcct gccgcacctg aacggcccgc tgcacctggc
     2881 tccgatgacc ctgcgttgta ccgtgggttc tccgccgcgt cgtgtggact gtaccattca
     2941 tcgtccgtct aacggcggcc tgatcgtaga actggaacca gcaaccaaga ccactaacat
     3001 tgcgccggct ctggacggtg cgtttcatcg tatcacttct tcatcctccc tgatgggcct
     3061 gtgtgacgaa accgcgacta ttatccgtga gattactggc tacgaccgtg tgatggtagt
     3121 acgtttcgat gaagagggta atggcgaaat tctgtccgaa cgtcgtcgtg cggacctgga
     3181 agcgttcctg ggtaaccgct acccggcgtc tactattccg cagatcgctc gtcgcctgta
     3241 cgaacataac cgtgttcgcc tgctggtaga tgtgaactat actccggttc cgctacagcc
     3301 gcgcatcagc ccgctgaacg gtcgtgatct ggatatgtcc ctgtcttgcc tgcgctctat
     3361 gtccccgatc caccagaaat acatgcagga catgggcgtt ggcgcgaccc tggtttgctc
     3421 tctgatggtg tctggtcgtc tgtggggtct gatcgcttgc caccactacg aaccgcgctt
     3481 cgttccgttc cacattcgcg ctgctggcga agcgctggcg gaaacttgtg cgatccgcat
     3541 cgcgacgctg gagagctttg cacagtctca gtccaaagag ggcaggggga gcctcctaac
     3601 ttgcggagac gtcgaggaga atcctggccc atctagactg gacaagagca aagtcataaa
     3661 cggagctctg gaattactca atggtgtcgg tatcgaaggc ctgacgacaa ggaaactcgc
     3721 tcaaaagctg ggagttgagc agcctaccct gtactggcac gtgaagaaca agcgggccct
     3781 gctcgatgcc ctgccaatcg agatgctgga caggcatcat acccacttct gccccctgga
     3841 aggcgagtca tggcaagact ttctgcggaa caacgccaag tcataccgct gtgctctcct
     3901 ctcacatcgc gacggggcta aagtgcatct cggcacccgc ccaacagaga aacagtacga
     3961 aaccctggaa aatcagctcg cgttcctgtg tcagcaaggc ttctccctgg agaacgcact
     4021 gtacgctctg tccgccgtgg gccactttac actgggctgc gtattggagg aacaggagca
     4081 tcaagtagca aaagaggaaa gagagacacc taccaccgat tctatgcccc cacttctgag
     4141 acaagcaatt gagctgttcg accggcaggg agccgaacct gccttccttt tcggcctgga
     4201 actaatcata tgtggcctgg agaaacagct aaagtgcgaa agcggcgggg gtagcggcag
     4261 cggtagcgca tgcagctccc aaagccatcc agatggactt tctggccgag accagccagt
     4321 ggagctgctg aatcctgccc gcgtgaacca catgcccagc acggtggatg tggccacggc
     4381 gctgcctctg caagtggccc cctcggcagt gcccatggac ctgcgcctgg accaccagtt
     4441 ctcactgcct gtggcagagc cggccctgcg ggagcagcag ctgcagcagg agctcctggc
     4501 gctcaagcag aagcagcaga tccagaggca gatcctcatc gctgagttcc agaggcagca
     4561 cgagcagctc tcccggcagc acgaggcgca gctccacgag cacatcaagc aacaacagga
     4621 gatgctggcc atgaagcacc agcaggagct gctggaacac cagcggaagc tggagaggca
     4681 ccgccaggag caggagctgg agaagcagca ccgggagcag aagctgcagc agctcaagaa
     4741 caaggagaag ggcaaagaga gtgccgtggc cagcacagaa gtgaagatga agttacaaga
     4801 atttgtcctc aataaaaaga aggcgctggc ccaccggaat ctgaaccact gcatttccag
     4861 cgaccctcgc tactggtacg ggaaaacgca gcacagttcc cttgaccaga gttctccacc
     4921 ccagagcgga gtgtcgacct cctataacca cccggtcctg ggaatgtacg acgccaaaga
     4981 tgacttccct cttaggaaaa cagcttctga accgaatctg aaattacggt ccaggctaaa
     5041 gcagaaagtg gccgaaagac ggagcagccc cctgttacgc aggaaagacg ggccagtggt
     5101 cactgctcta aaaaagcgtc cgttggatgt cacagactcc gcgtgcagca gcgccccagg
     5161 ctccggaccc agctcaccca acaacagctc cgggagcgtc agcgcggaga acggtatcgc
     5221 gcccgccgtc cccagcatcc cggcggagac gagtttggcg cacagacttg tggcacgaga
     5281 aggctcggcc gctccacttc ccctctacac atcgccatcc ttgcccaaca tcacgctggg
     5341 cctgcctgcc accggcccct ctgcgggcac ggcgggccag caggacaccg agagactcac
     5401 ccttcccgcc ctccagcaga ggctctccct tttccccggc acccacctca ctccctacct
     5461 gagcacctcg cccttggagc gggacggagg ggcagcgcac agccctcttc tgcagcacat
     5521 ggtcttactg gagcagccac cggcacaagc acccctcgtc acaggcctgg gagcactgcc
     5581 cctccacgca cagtccttgg ttggtgcaga ccgggtgtcc ccctccatcc acaagctgcg
     5641 gcagcaccgc ccactggggc ggacccagtc ggccccgctg ccccagaacg cccaggctct
     5701 gcagcacctg gtcatccagc agcagcatca gcagtttctg gagaaacaca agcagcagtt
     5761 ccagcagcag caactgcaga tgaacaagat catccccaag ccaagcgagc cagcccggca
     5821 gccggagagc cacccggagg agacggagga ggagctccgt gagcaccagg ctctgctgga
     5881 cgagccctac ctggaccggc tgccggggca gaaggaggcg cacgcacagg ccggcgtgca
     5941 ggtgaagcag gagcccattg agagcgatga ggaagaggca gagcccccac gggaggtgga
     6001 gccgggccag cgccagccca gtgagcagga gctgctcttc agacagcaag ccctcctgct
     6061 ggagcagcag cggatccacc agctgaggaa ctaccaggcg tccatggagg ccgccggcat
     6121 ccccgtgtcc ttcggcggcc acaggcctct gtcccgggcg cagtcctcac ccgcgtctgc
     6181 caccttcccc gtgtctgtgc aggagccccc caccaagccg aggttcacga caggcctcgt
     6241 gtatgacacg ctgatgctga agcaccagtg cacctgcggg agtagcagca gccaccccga
     6301 gcacgccggg aggatccaga gcatctggtc ccgcctgcag gagacgggcc tccggggcaa
     6361 atgcgagtgc atccgcggac gcaaggccac cctggaggag ctacagacgg tgcactcgga
     6421 agcccacacc ctcctgtatg gcacgaaccc cctcaaccgg cagaaactgg acagtaagaa
     6481 acttctaggc tcgctcgcct ccgtgttcgt ccggctccct tgcggtggtg ttggggtgga
     6541 cagtgacacc atatggaacg aggtgcactc ggcgggggca gcccgcctgg ctgtgggctg
     6601 cgtggtagag ctggtcttca aggtggccac aggggagctg aagaatggct ttgctgtggt
     6661 ccgcccccct ggacaccatg cggaggagag cacgcccatg ggcttttgct acttcaactc
     6721 cgtggccgtg gcagccaagc ttctgcagca gaggttgagc gtgagcaaga tcctcatcgt
     6781 ggactgggac gtgcaccatg gaaacgggac ccagcaggct ttctacagcg accctagcgt
     6841 cctgtacatg tccctccacc gctacgacga tgggaacttc ttcccaggca gcggggctcc
     6901 tgatgaggtg ggcacagggc ccggcgtggg tttcaacgtc aacatggctt tcaccggcgg
     6961 cctggacccc cccatgggag acgctgagta cttggcggcc ttcagaacgg tggtcatgcc
     7021 gatcgccagc gagtttgccc cggatgtggt gctggtgtca tcaggcttcg atgccgtgga
     7081 gggccacccc acccctcttg ggggctacaa cctctccgcc agatgcttcg ggtacctgac
     7141 gaagcagctg atgggcctgg ctggcggccg gattgtcctg gccctcgagg gaggccacga
     7201 cctgaccgcc atttgcgacg cctcggaagc atgtgtttct gccttgctgg gaaacgagct
     7261 tgatcctctc ccagaaaagg ttttacagca aagacccaat gcaaacgctg tccgttccat
     7321 ggagaaagtc atggagatcc acagcaagta ctggcgctgc ctgcagcgca caacctccac
     7381 agcggggcgt tctctgatcg aggctcagac ttgcgagaac gaagaagccg agacggtcac
     7441 cgccatggcc tcgctgtccg tgggcgtgaa gcccgccgaa aagagaccag atgaggagcc
     7501 catggaagag gagccgcccc tgtaaggtaa atttaaatcg gcctcgactg tgccttctag
     7561 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     7621 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     7681 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     7741 caggcatgct ggggacgcgg ccgcaattta acgcgaatta attctgtgga atgtgtgtca
     7801 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     7861 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     7921 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     7981 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     8041 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     8101 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     8161 tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag
     8221 gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac
     8281 gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag
     8341 gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac
     8401 caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac
     8461 gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc
     8521 gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc
     8581 gtgcacttcg tggccgagga gcaggactga gtcgacaatc aacctctgga ttacaaaatt
     8641 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     8701 gctttaatgc ctttgtatca tgcgttaact aaacttgttt attgcagctt ataatggtta
     8761 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     8821 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggaattgac tcaaatgatg
     8881 tcaattagtc tatcagaagc tcatctggtc tcccttccgg gggacaagac atccctgttt
     8941 aatatttaaa cagcagtgtt cccaaactgg gttcttatat cccttgctct ggtcaaccag
     9001 gttgcagggt ttcctgtcct cacaggaacg aagtccctaa agaaacagtg gcagccaggt
     9061 ttagccccgg aattgactgg attccttttt tagggcccat tggtatggct ttttccccgt
     9121 atccccccag gtgtctgcag gctcaaagag cagcgagaag cgttcagagg aaagcgatcc
     9181 cgtgccacct tccccgtgcc cgggctgtcc ccgcacgctg ccggctcggg gatgcggggg
     9241 gagcgccgga ccggagcgga gccccgggcg gctcgctgct gccccctagc gggggaggga
     9301 cgtaattaca tccctggggg ctttgggggg gggctgtccc tgatatctat aacaagaaaa
     9361 tatatatata ataagttatc acgtaagtag aacatgaaat aacaatataa ttatcgtatg
     9421 agttaaatct taaaagtcac gtaaaagata atcatgcgtc attttgactc acgcggtcgt
     9481 tatagttcaa aatcagtgac acttaccgca ttgacaagca cgcctcacgg gagctccaag
     9541 cggcgactga gatgtcctaa atgcacagcg acggattcgc gctatttaga aagagagagc
     9601 aatatttcaa gaatgcatgc gtcaatttta cgcagactat ctttctaggg ttaatctagc
     9661 tgcatcagga tcatatcgtc gggtcttttt tccggctcag tcatcgccca agctggcgct
     9721 atctgggcat cggggaggaa gaagcccgtg ccttttcccg cgaggttgaa gcggcatgga
     9781 aagagtttgc cgaggatgac tgctgctgca ttgacgttga gcgaaaacgc acgtttacca
     9841 tgatgattcg ggaaggtgtg gccatgcacg cctttaacgg tgaactgttc gttcaggcca
     9901 cctgggatac cagttcgtcg cggcttttcc ggacacagtt ccggatggtc agcccgaagc
     9961 gcatcagcaa cccgaacaat accggcgaca gccggaactg ccgtgccggt gtgcagatta
    10021 atgacagcgg tgcggcgctg ggatattacg tcagcgagga cgggtatcct ggctggatgc
    10081 cgcagaaatg gacatggata ccccgtgagt tacccggcgg gcgcgcttgg cgtaatcatg
    10141 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
    10201 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
    10261 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
    10321 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
    10381 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
    10441 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
    10501 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
    10561 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
    10621 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
    10681 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
    10741 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
    10801 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    10861 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    10921 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    10981 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    11041 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    11101 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    11161 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    11221 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    11281 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    11341 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    11401 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    11461 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    11521 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    11581 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    11641 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    11701 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    11761 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    11821 gccatccgta agatgctttt c
//
