LOCUS       sequence-355456-        9298 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Psra-11::UNC-7-UrSL-wCherry unc-54 3' UTR C.elegans neural 
            expression of UNC-7-wCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9298)
  AUTHORS   Kawano T, Po MD, Gao S, Leung G, Ryu WS, Zhen M
  TITLE     An imbalancing act: gap junctions reduce the backward motor circuit 
            activity to bias C. elegans for forward locomotion.
  JOURNAL   Neuron. 2011 Nov 17;72(4):572-86. doi: 10.1016/j.neuron.2011.09.005.
  PUBMED    22099460
REFERENCE   2  (bases 1 to 9298)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2011.09"; journalName: "Neuron"; date: 
            "2011-11-17- 17"; volume: "72"; issue: "4"; pages: "572-86"
COMMENT     Sequence Label: pJH2037
FEATURES             Location/Qualifiers
     source          1..9298
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    11..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     16..38
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     35..51
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     35..51
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(4255..4266)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
     primer_bind     complement(6918..6940)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7040..7059
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7137..7155)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7223..7327
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7328..7396,7397..8188)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7546..7565)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8359..8947
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8848..8867
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9101..9118
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9235..9256
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9271..9276,9277..9294,9295..9298,1..3)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
       61 gccaagctgt aagtttaaac atgatcttac taactaacta ttctcattta aattttcaga
      121 gcttaaaaat ggctgaaatc actcacaacg atggatacgc taacaacttg gaaatgaaat
      181 aagcttgggc tgcagcatgc ggccgcacgt tttcccttta cattagcaaa accgaccaac
      241 gaccgaaacc taagcattat ccaggcctaa acatatttta aaaaaagtgt agtttaccaa
      301 atgggagaca ttttccttgt tctttgtggg atgcttttga aattgaatag aaatctctag
      361 attaatcaat gtgtttgctc cgatgcgcct agtctcaaag ccttctcatg ttgcaaacaa
      421 atgcactaac taaactaatt aacaagtcac ggaggagatt caaaacattt cacttttcag
      481 gaaagtagca tttttaaata tattcatgtt gtagatttgt cattttgcaa tattttttat
      541 caaaattgga ttacaccctc ttttgagcag tctgacttgt tggctgactt gattttcttt
      601 taaagtgatg aaattttatt catatttgat ttttagtaaa taaattattg caaaccccat
      661 tatgcaactt tgacagctta tatctcagtt gccgtttgtt ttatgaaaaa ttgtcaactt
      721 accaaataat tcttagttac gatctgaaat ttttcgaaaa ctctaatctt tagtcaaaaa
      781 tgttcagcaa tgtaaacatt ttttcattac aaaaaaagat gatgtagatg catacatcaa
      841 gtatctcaac cggaacaccc cctattttgc accatctctg tcatcttttt cttccttttt
      901 cagatgggaa acatacaatt aatgctcaca tgtcatgtct agtcatttgt ttttttttgt
      961 ttgtttttaa tccgaatctc ttattcatct ttagacgttc cattcggcag aggcgagttg
     1021 gggcgcctct tactcgcctc aaattttgtg tttgcactca taatcaaaga ataaattatc
     1081 ttgcccattt tggtccttga ccatttgtgt aggcaatagg caggcagaca tattttctta
     1141 aacagaagac cgaactgtga gtgtacaaaa ataaattaaa tattacctac ctttgttaga
     1201 atttggaata agattaaaaa attcgttgtc ctctgtggaa ttgctcatta ttagattttt
     1261 acgaaatcat gcggattaga aagtttaata tttatgtaca gtgcgtccaa cttctatagc
     1321 acccccctcc aattcggccg tttggagctt ttcaaaatat ttttcggaaa agtgttagtg
     1381 cagttcgata gcaaatattg aaaaacctat gctccccaat ttttatcatg atatctcaaa
     1441 aatcacggaa attgggtcaa aatatctgaa aaatggccgt cgaacttcta tagcaccccc
     1501 tctgattttt gacaaatttc tgtaaattcg gggttttctt cgtagttaat gaatttattt
     1561 tgtaaataac ttcacaccaa aataatcagg tggtttcact ttcacattta aaaaataaat
     1621 ataacacttg aaaacacgtg gaatgcctgg gattgatcaa aaattgatca aaatgttctt
     1681 ctccgtctat tcattactat gtaattagtt gtagaaagca tttattcata tgaaccaaat
     1741 ggtaaaggga gcaacttcag tggtttttaa aaggctcgat ttaaccatga aaacgagcgt
     1801 tcggaggcac aaagctgaag gttttgactt cgtattgtac agaacttcag ctcatcctgc
     1861 tcaaatgtct ttgaagcttc gattttgatt agatgtcttt gaaaatttat acggaaaaga
     1921 tcatgagacc gctcaaaaag aggacaaagg caaagcgagg ccgcaagtct ttttttcaag
     1981 ctaacatgcg aaaattttag acagaaaagt aagatatacc tttccaaatg acgattttgc
     2041 tagttgtatt tataactcaa gttaaattta gcgaattcgc tcattgaatc tttttttggt
     2101 caagtaatgc aaaagctacc agacaaaaca ttgaacctgc gattgtagat gtctggcatc
     2161 tgatttaccc atggatttcc acggattttt tgttcatata aaataatttt taatttttgc
     2221 tataatgtag taatttttag tcgaaaatgt attcccaacc attatctctg cgcctccgaa
     2281 cgctcgtttt catggttaaa tcgaaccttt taaaaaccac tgaagttgct ccctttacca
     2341 tttggttcat atgaataaat gctttctaca actaattata tagtaatgaa tagacggaga
     2401 agaacatttt gatctatttt ggtgctgcga aaatcattgg gagccaagat atggcctctg
     2461 aagcaaataa gaacgttgag gggggtgcta tagaagttgg acgcactgta tttccaatta
     2521 ttttttaaga atttcgattt tataaacagc ctttgctccg cctatttgag ctcatgatgt
     2581 ttctcttttg ttttcaagat tgttgattcc ttcctattgt cctattgtga tttttaaatt
     2641 tttaaattgc tcattaactt gatttttatt tagtacatat ctctaatatg ttgtttcaca
     2701 ttgcaccaac cggtctcaat ttttgaaatt atttattgtt atgtttgaat attttaaaca
     2761 aatttaaaaa tttagaaaaa ttgctgagct gttgcctaaa ctcataagtg tttcaaaatt
     2821 tgctacgatt ttgcatgtac tttttaatat tttatatgct ttttcgtatt ccaaaacaaa
     2881 caatttagtt tactttacaa attatgaaca aaaaaaattt gatgtaacgg agagaaaatt
     2941 attaaacgtt acatgtattt ttcgatcgta tcgagaaaaa tgcaaaaaat atcaaattgt
     3001 aatttttccg cacatctccg caacacacaa ttcccgggat tggccaaagg acccaaaggt
     3061 atgtttcgaa tgatactaac ataacataga acattttcag gaggaccctt ggctagcgtc
     3121 gacggatccc catgctcggc tcctccagca atcctgaacc gccactcctg tcgcggataa
     3181 tcggagtacc tccaccgccg ccgccacgag caccgaccac agctctcgtc cttcgggtgc
     3241 ccaccgtcga cagtccatcg aaaaagaaac aacagccaga cactaggaac aaatatcagg
     3301 aaactgcgct cagggataaa aaaactcgga caccacttga aaaagccaga catctcgaca
     3361 atcttccctc gtatcaggcc caaaaactat tagacggttc gcaccagctt cgcatcgact
     3421 cccaccacgt tggtagtgca ggtcacggtg caggacaagg tcacggacac aagaaagagt
     3481 ttggacctgc aatgattctc tattatttag cttctgcttt tcgggcgctc tatccgcgtc
     3541 tagatgatga ttttgtggac aaacttaatt attattatac tactacaata ttagcttcct
     3601 ttgcacttct tgtttccgcc aagcagtatg tcggttttcc catccaatgt tgggtgcccg
     3661 ccacattcac agatgccatg gagcagtaca ctgagaacta ctgctgggta cagaacacat
     3721 actgggttcc catgcaagag gacattccac gtgaaattta ttcgcgtaga aatcgacaaa
     3781 ttggctatta ccaatgggtg ccgtttattc ttgctattga ggccttgttg ttctacgttc
     3841 catgcattct ctggagagga ttgttgtatt ggcacagtgg tatcaaccta caagggctag
     3901 tccagatggc gtgtgacgca cgactgatgg attcggaaat aaagacgaga acagtctaca
     3961 caatggcccg gcacatgcaa gacgaggttc agctcaccaa tatcgaccgg caaggacaca
     4021 gtcggagttg tttctcgaat ttgcaacttg gcgcaaactg tgggagacat tgtggttgct
     4081 acgtcactat gctctacata ggaatcaagg ttctctactc agcaaatgtg ttgctccagt
     4141 ttttcttgct gaaccatctt cttgggtcta acgacttggc ttatggattc tctttgttga
     4201 aagacttgat gcacgcgatt gagtgggaac agactggaat gttccccagg gtcacacttt
     4261 gtgattttga ggttcgagtt ctgggaaaca ttcatcgaca taccgttcag tgcgtgttaa
     4321 tgatcaatat gtttaatgag aaaattttct tattcctctg gttttggttc ttaacctgtg
     4381 gaattgttac cgtatgtaac actatgtact ggattctcat tatgttcatt ccgtctcagg
     4441 gaatgtcatt tgtccgcaaa tacctccgtg ttctacctga ccaccctgcg aaaccaatag
     4501 ctgatgacgt cacgctgagg aagttcacta ataacttcct ccgcaaagat ggagtcttca
     4561 tgttgcgaat gatttccaca cacgccggcg aactgatgag cagtgagctc attcttgctc
     4621 tctggcagga tttcaacaac gtcgaccgca gtccgacgca attttgggat gcggaacacg
     4681 gtcaagggac gatagactga cggatccccg agatcttcag cgctcagcat tcgtagaatt
     4741 gtaagtttaa actttctcat actaactaac gcgctctatt taaattttca gaattcgctg
     4801 tctcatccta ctttcaccta gttaactgct tgtcttaaaa tctatgcttc tctttagtat
     4861 ctaaaatttt cctagaagct tacaagtata taaatggtct cttctcaata aaggttgtat
     4921 atttattcat cttattgaat ctgccatttc ctcgtttttg cgagtttata taccttccaa
     4981 ttttctttct attgtatttt caacttctaa ttttaattca gggaaactgc ttcaacgcat
     5041 cggtacccta tggtctcaaa gggtgaagaa gataacatgg caattattaa agagtttatg
     5101 cgtttcaagg tgcatatgga gggatctgtc aatgggcatg agtttgaaat tgaaggtgaa
     5161 ggagaaggcc gaccatatga gggaacacaa accgcaaaac taaaggtaag tttaaacata
     5221 tatatactaa ctaaccctga ttatttaaat tttcaggtaa ctaaaggcgg accattacca
     5281 ttcgcctggg acatcctctc tccacagttc atgtatggaa gtaaagctta tgttaaacat
     5341 ccggcagata taccagatta tttgaaactt tcattcccgg agggttttaa gtgggaacgc
     5401 gtaatgaatt ttgaagacgg aggagttgtt acagtgacgc aagactcaag gtaagtttaa
     5461 acagttcggt actaactaac catacatatt taaattttca gcctccaaga tggagaattt
     5521 atttataaag tcaaacttcg aggaacgaat ttcccctcgg atggatctgt tatgcagaag
     5581 aagactatgg gatgggaagc ttcaagtgaa agaatgtacc ctgaagacgg tgctcttaag
     5641 ggagagatta aacaacgtct taaattgaaa gatggaggac attacgatgc tgaggtaagt
     5701 ttaaacatga ttttactaac taactaatct gatttaaatt ttcaggtgaa gacaacttac
     5761 aaagccaaaa aaccagttca gctgccagga gcgtacaatg ttaatattaa actggatatc
     5821 acctcccaca acgaggatta cactatcgtt gagcaatatg aaagagctga agggcggcac
     5881 tcgacaggtg gcatggatga attgtataag taggagctcc cgagatcttc agcgctcagc
     5941 attcgtagaa ttgtaagttt aaactttctc atactaacta acgcgctcta tttaaatttt
     6001 cagaattcca actgagcgcc ggtcgctacc attaccaact tgtctggtgt caaaaataat
     6061 aggggccgct gtcatcagag taagtttaaa ctgagttcta ctaactaacg agtaatattt
     6121 aaattttcag catctcgcgc ccgtgcctct gacttctaag tccaattact cttcaacatc
     6181 cctacatgct ctttctccct gtgctcccac cccctatttt tgttattatc aaaaaacttc
     6241 tcttaatttc tttgtttttt agcttctttt aagtcacctc taacaatgaa attgtgtaga
     6301 ttcaaaaata gaattaattc gtaataaaaa gtcgaaaaaa attgtgctcc ctccccccat
     6361 taataataat tctatcccaa aatctacaca atgttctgtg tacacttctt atgtttttta
     6421 cttctgataa atttttttga aacatcatag aaaaaaccgc acacaaaata ccttatcata
     6481 tgttacgttt cagtttatga ccgcaatttt tatttcttcg cacgtctggg cctctcatga
     6541 cgtcaaatca tgctcatcgt gaaaaagttt tggagtattt ttggaatttt tcaatcaagt
     6601 gaaagtttat gaaattaatt ttcctgcttt tgctttttgg ggtttcccct attgtttgtc
     6661 aagatttcga ggacggcgtt tttcttgcta aaatcacaag tattgatgag cacgatgcaa
     6721 gaaagatcgg aagaaggttt gggtttgagg ctcagtggaa ggtgagtaga agttgataat
     6781 ttgaaagtgg agtagtgtct atggggtttt tgccttaaat gacagaatac attcccaata
     6841 taccaaacat aactgtttcc tactagtcgg ccgtacgggc cctttcgtct cgcgcgtttc
     6901 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     6961 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     7021 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     7081 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcggcc ttaagggcct
     7141 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     7201 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     7261 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     7321 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     7381 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     7441 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     7501 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     7561 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     7621 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     7681 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     7741 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     7801 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     7861 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     7921 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     7981 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     8041 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     8101 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     8161 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     8221 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     8281 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     8341 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     8401 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     8461 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     8521 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     8581 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     8641 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     8701 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     8761 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     8821 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     8881 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     8941 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     9001 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     9061 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     9121 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     9181 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     9241 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatg
//
