LOCUS       sequence-355460-        9445 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pacr-2::UNC-9-UrSL-wCherry unc-54 3' UTR C.elegans neural expression
            of UNC-9-wCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9445)
  AUTHORS   Kawano T, Po MD, Gao S, Leung G, Ryu WS, Zhen M
  TITLE     An imbalancing act: gap junctions reduce the backward motor circuit 
            activity to bias C. elegans for forward locomotion.
  JOURNAL   Neuron. 2011 Nov 17;72(4):572-86. doi: 10.1016/j.neuron.2011.09.005.
  PUBMED    22099460
REFERENCE   2  (bases 1 to 9445)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2011.09"; journalName: "Neuron"; date: 
            "2011-11-17- 17"; volume: "72"; issue: "4"; pages: "572-86"
COMMENT     Sequence Label: pJH1897
FEATURES             Location/Qualifiers
     source          1..9445
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(6989..7011)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7111..7130
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7208..7226)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7294..7398
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7399..7467,7468..8259)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7617..7636)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8430..9018
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8919..8938
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9172..9189
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9306..9327
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9342..9347,9348..9365,9366..9372)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9380..9396
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9385..9407
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9404..9420
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9404..9420
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaacatgatc ttactaacta actattctca tttaaatttt cagagcttaa aaatggctga
       61 aatcactcac aacgatggat acgctaacaa cttggaaatg aaataagctt gggctgcagc
      121 atgcggccgc attcttggaa tttcgcgttt tccattttcc gttgtttttt cattttataa
      181 aagtttcagc acctacattt ttcaatattt cttcaatgga gaataaatca gttgaatcag
      241 ttgaaaaaca aagtcagagc tgtggaagtc gaaatttaag agcttttttt ttaatttttt
      301 atagttgttg taaagcttat tagtataccg cacctactgt taatatgaac catcaaatgt
      361 tactcttcct ttttcattaa aaaaaacttt cagtttgccc cgccggtgcg attccttata
      421 agatgccaca agttttgtta tgcgacccga caattgttaa tatccgtcca tacgactata
      481 aggtttggtt ttattcgcat tttattgttt tgtttttgga tcaactctat caaaaaaaca
      541 atattcgttc atgatactaa tgactaatga gttttcagtg cgttgaagca gcaaacggac
      601 aatatcttcc tgccgatggc cgatctcttt gctgcaagac caccactctg tactcgtttg
      661 cctcggtttt tggagaggct aaaataagcc ctcggctagt gccaaatccc cctctttcag
      721 cgatcgaata tgtgcgtcgg tattttttgt tcctcttatt ttttgtctta gacatcaacg
      781 ccgccgccgt aaaacagaag acatgtttcc gtacttccag gtcactttga acgttcacac
      841 atccgccaaa gagcactcac cggaaattag aactggagat cattttgtgc tgacaccata
      901 caaactctac gagccggcat atttgaaaaa aatcaaactg ttcacagact ttggtcatgg
      961 ctcatttgtg catgttatac tttttggtaa atctattgaa catgttcgga gtgttcatgc
     1021 gagcgtaact aagttttgag aaaaaaagaa tgagagaata actagggctt ttgtcttttt
     1081 ttgtgaatat ctggttatgt ttgaagatcc aatgtcaaca actgaaacaa tgcaacttta
     1141 ttatgaccga ccaacatcca atgggaaaac tatcaattta gatgagccaa ttccagacgg
     1201 aggtttcaat aattaaaaaa aaattaagaa taatcaaata acatgcgctt ttcaggattc
     1261 atttctaaga aaatattcaa tgcggctcca attacacaaa tagaaaaccc atcacgtccg
     1321 ggtccaataa aagaatgtga gaactttctt cttgaaaaat gaacatattg aattgcacca
     1381 aacgcaaaca agaaactcta aatttcagat cgaaaattgt ggattgtgct cattttcaag
     1441 accacaagcc cgtttggtcg agtttatatt ctttccacca aggatttgca cagcaagtat
     1501 agatcagtca ccgatttttt gaaatccgac acaggaaaca tgctaggaac tcctgtggct
     1561 ggaacttatt tttatgtgag ttttgttttg aaccctcaac tataaaattg tcggcccgtt
     1621 ttgttttcag ctcaccaccg actgattttc atttcaaaat taatgcagat ttctacgtgt
     1681 tctttttcaa ataaatttat atattttttg ttctctcaca aaataaataa tttcgagagt
     1741 taagtgatca ttctcagaca tattctataa ttgtccgtca ggaaacagat agaggaacct
     1801 ttgataaata aattataaaa ggccgacagg gaaatgcttc tccctcgact ctgttagaag
     1861 cgaagaggca atttttaaaa atttcccatt tttatgcgtt tttctgattt ccaaagtcac
     1921 gcatcattta ccaaccgcaa cgattcataa ggacgtaatg ttgattttct ggacgatttt
     1981 tatatagttt tgatgtgaaa ttcgctttat ttgtctgaaa gtggaacaga tttagtgtgg
     2041 atttaaccga aaaatgcaga tcttttaaaa atttaaacca gtgttttgtt gccttatttg
     2101 cgaaaaaaaa ttgtgatatt tcgagtattg ctgcaatttg attagtaaaa tgttaggata
     2161 taagtattta aaaagtataa ttggttgaat tttctttttc atcaccgact tccaaaaata
     2221 tagagatact gagttttcac cattttttga atgtaaataa aaacattttg aacacttgtg
     2281 ctgggattca atccaaatac attcactgtc atttaatatt attatatatc aaaataaaac
     2341 gcataacttt ttctgttttt ttttaaattt tttcaaaatg taggtttggt aagttaaggt
     2401 aaggttaggc aatgtaggta aacacgagat gatactccac gttgaaaatt gttttcataa
     2461 atacagttta gattgaactg aaggatttta tcgtctaata gaatataatt ttttagttca
     2521 agatttagga tttgttttca ctttttttta gattcaagac atatatctcg caaaatgagt
     2581 tttgtctttg agccatcttg tcatcatagc acattgaatt ttgttgttcg caagacggaa
     2641 tatatgttcc cagctgcaaa ttttgtgtcg gatatttctt ctttagcgaa aggcgttaaa
     2701 aaccaaaaat agtcgaagcg tttgatatgt gtcccgttgg tttttcgtgt ccatctattc
     2761 tgtttcgagt acacatgttg cgacgcggca ctgatgaaaa agtgaaagag ctagtgtcac
     2821 acacaaacac taagtatgct catgtgagtg tgtagatatg tgtagatgta tgtcctgaag
     2881 gaataaaaag actttttgaa gctacatttt taaacaaaaa aagacagaaa catgaagcac
     2941 aactctctat tctgtcgaac ggcgtgtgca actcacgcac acccaaaaat atgaaatata
     3001 acattcgcac caacaaagcg cgaatatcaa tgaatctcga gggaacgatg gctgcctgcc
     3061 ggcgcgtgtt gctcttctgt cattgggata actggcgggt ctgttgggtc cgtcctttag
     3121 accatttgac tacactgccc tgcatatgtg tgaaacgtcg tcgttgctgt tgggagctgt
     3181 ctgtgtgtat gtgtgtgtgt gtgagtatgg agcagtaaaa agctgccgat acgagaagcg
     3241 tcgccccgga cggacggcga tggcgcatcg ccaaatgtgt cattttagtt gtcttcacga
     3301 accagagggc ccacgacgga cgggcggcca cgcccacctc aaccagaata tccttccttc
     3361 tcccacccac ccttgcggac gcgcggatgc gctgaacaaa aaggcgacga agaagaagaa
     3421 gaagaagctg aacgaagaaa aaagcatcaa aagaagtgaa gcggctctac caccccgacc
     3481 accttcctcc acaggaagga cgccgttttc atcccgggat tggccaaagg acccaaaggt
     3541 atgtttcgaa tgatactaac ataacataga acattttcag gaggaccctt ggctagcgtc
     3601 gacggatccg gatgagtatg ctattgtatt atttcgcgag tgctgtgaag tcaatacagt
     3661 ttcacgtcga tgatgacatt attgacaagc ttaactacta ttacaccaca gccattatca
     3721 cagtgttcgc gattctcgtc tctgctaaac agtatgtcgg ttttccaatc caatgttggg
     3781 tacctgcgac gttcaccgag cccatggagc agtacacgga aaactattgt tgggttcaaa
     3841 acacctactt tctcccttta cacgactaca ttccacataa ctatgcagaa cgtgaaaatc
     3901 gacaaatcgg gtattatcaa tgggttccgt ttgtgttagc tcttgaagct cttcttttct
     3961 atgtgccgac tattgtatgg agactgctca gctggcaatc aggaattcat gtgcagtctc
     4021 tggttcaaat ggcttgtgac tccagattgt tggatttgga atcgaggaac agagcgttgc
     4081 aaaccatcgc aacaaatgta gaagaagcgc tacacgtgaa acatcaagtg atgtccggaa
     4141 atcgtctgaa actgctcaac ttgattattt gcacacgatc ttccggtgct gctgtcacgt
     4201 tcctctatat ctctgtcaaa attctgtaca ccgtgaacat cgtcggccaa atctttctac
     4261 tgaacacgtt tctcggtaac cgtagcaaat ggtacggact gcaagttctc aacgacttga
     4321 tgaacggacg agaatgggag gagtctggtc attttccacg tgtaacgtta tgtgactttg
     4381 aagtaaaagt actgggaaac gtgcaccgac atacagtaca atgtgtactt atgattaaca
     4441 tgttcaacga gaagatcttt ctgttcttgt ggttctggta cttccttctt gccggtgcta
     4501 ctttgtgctc gcttttctac tggatctaca tttcagtggt gccaagcagg cagctcaact
     4561 ttgttggaaa gtatctgact ggaatcgagg gatataaaat ggtggattcg caatcactac
     4621 gacgttttgt atttcacttt ttaagacagg atggtgtttt cctacttcgt atggttgcaa
     4681 ctcacgccgg agaactaccc tgttacgagc ttgcgaaaac gttatggaac aactactgtg
     4741 acaataaaga aggaaaaatg cacgacgtgt gaggatcccc gagatcttca gcgctcagca
     4801 ttcgtagaat tgtaagttta aactttctca tactaactaa cgcgctctat ttaaattttc
     4861 agaattcgct gtctcatcct actttcacct agttaactgc ttgtcttaaa atctatgctt
     4921 ctctttagta tctaaaattt tcctagaagc ttacaagtat ataaatggtc tcttctcaat
     4981 aaaggttgta tatttattca tcttattgaa tctgccattt cctcgttttt gcgagtttat
     5041 ataccttcca attttctttc tattgtattt tcaacttcta attttaattc agggaaactg
     5101 cttcaacgca tcggtaccct atggtctcaa agggtgaaga agataacatg gcaattatta
     5161 aagagtttat gcgtttcaag gtgcatatgg agggatctgt caatgggcat gagtttgaaa
     5221 ttgaaggtga aggagaaggc cgaccatatg agggaacaca aaccgcaaaa ctaaaggtaa
     5281 gtttaaacat atatatacta actaaccctg attatttaaa ttttcaggta actaaaggcg
     5341 gaccattacc attcgcctgg gacatcctct ctccacagtt catgtatgga agtaaagctt
     5401 atgttaaaca tccggcagat ataccagatt atttgaaact ttcattcccg gagggtttta
     5461 agtgggaacg cgtaatgaat tttgaagacg gaggagttgt tacagtgacg caagactcaa
     5521 ggtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc agcctccaag
     5581 atggagaatt tatttataaa gtcaaacttc gaggaacgaa tttcccctcg gatggatctg
     5641 ttatgcagaa gaagactatg ggatgggaag cttcaagtga aagaatgtac cctgaagacg
     5701 gtgctcttaa gggagagatt aaacaacgtc ttaaattgaa agatggagga cattacgatg
     5761 ctgaggtaag tttaaacatg attttactaa ctaactaatc tgatttaaat tttcaggtga
     5821 agacaactta caaagccaaa aaaccagttc agctgccagg agcgtacaat gttaatatta
     5881 aactggatat cacctcccac aacgaggatt acactatcgt tgagcaatat gaaagagctg
     5941 aagggcggca ctcgacaggt ggcatggatg aattgtataa gtaggagctc ccgagatctt
     6001 cagcgctcag cattcgtaga attgtaagtt taaactttct catactaact aacgcgctct
     6061 atttaaattt tcagaattcc aactgagcgc cggtcgctac cattaccaac ttgtctggtg
     6121 tcaaaaataa taggggccgc tgtcatcaga gtaagtttaa actgagttct actaactaac
     6181 gagtaatatt taaattttca gcatctcgcg cccgtgcctc tgacttctaa gtccaattac
     6241 tcttcaacat ccctacatgc tctttctccc tgtgctccca ccccctattt ttgttattat
     6301 caaaaaactt ctcttaattt ctttgttttt tagcttcttt taagtcacct ctaacaatga
     6361 aattgtgtag attcaaaaat agaattaatt cgtaataaaa agtcgaaaaa aattgtgctc
     6421 cctcccccca ttaataataa ttctatccca aaatctacac aatgttctgt gtacacttct
     6481 tatgtttttt acttctgata aatttttttg aaacatcata gaaaaaaccg cacacaaaat
     6541 accttatcat atgttacgtt tcagtttatg accgcaattt ttatttcttc gcacgtctgg
     6601 gcctctcatg acgtcaaatc atgctcatcg tgaaaaagtt ttggagtatt tttggaattt
     6661 ttcaatcaag tgaaagttta tgaaattaat tttcctgctt ttgctttttg gggtttcccc
     6721 tattgtttgt caagatttcg aggacggcgt ttttcttgct aaaatcacaa gtattgatga
     6781 gcacgatgca agaaagatcg gaagaaggtt tgggtttgag gctcagtgga aggtgagtag
     6841 aagttgataa tttgaaagtg gagtagtgtc tatggggttt ttgccttaaa tgacagaata
     6901 cattcccaat ataccaaaca taactgtttc ctactagtcg gccgtacggg ccctttcgtc
     6961 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     7021 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     7081 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     7141 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcggc
     7201 cttaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     7261 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     7321 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     7381 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     7441 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     7501 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     7561 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     7621 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     7681 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     7741 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     7801 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     7861 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     7921 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     7981 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     8041 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     8101 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     8161 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     8221 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     8281 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     8341 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     8401 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     8461 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     8521 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     8581 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     8641 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     8701 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     8761 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     8821 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     8881 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     8941 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     9001 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     9061 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     9121 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     9181 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     9241 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     9301 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     9361 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     9421 catgattacg ccaagctgta agttt
//
