LOCUS       sequence-355467-        8507 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual fluorescent reporter construct with 1.2kb lambda spacer, 
            upstream TRE (Tetracycline Response Element) recruitment site, 
            phiC31 attB for integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8507)
  AUTHORS   Lensch S, Herschl MH, Ludwig CH, Sinha J, Hinks MM, Mukund A, 
            Fujimori T, Bintu L
  TITLE     Dynamic spreading of chromatin-mediated gene silencing and 
            reactivation between neighboring genes in single cells.
  JOURNAL   Elife. 2022 Jun 9;11. pii: 75115. doi: 10.7554/eLife.75115.
  PUBMED    35678392
REFERENCE   2  (bases 1 to 8507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-06-9"; volume: "11. pii: 75115. doi"; pages: " 
            10.7554/eLife.75115"
COMMENT     Sequence Label: 
            pMH010_phiC31_neo-5xTetO-pEF-H2B-mCitrine-1.2kb,lambda-pRSV-H2B-mChe
            rry
FEATURES             Location/Qualifiers
     source          1..8507
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      135..723
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     624..643
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    907..1041
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    1144..1265
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    1302..1371
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             1637..2440
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1700..1719)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2310..2329
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(2472..2489)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2478..2702
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    2775..2793
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2811..2829
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2847..2865
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2882..2900
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2918..2936
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             3029..3406
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      3425..3434
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3431..3433,3434..3436,3437..4147)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3476..3497)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3737..3756)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4084..4105
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4198..4217)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4199..4217)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    4491..4612
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        5922..6184
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     CDS             6198..6575
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      6588..6597
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6594..7307
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYKS"
     primer_bind     complement(6739..6757)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7007..7026
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    7423..7504
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        7547..7610
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7611..7679,7680..8471)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7829..7848)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
       61 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
      121 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
      181 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
      241 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
      301 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
      361 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
      421 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
      481 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
      541 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
      601 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
      661 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
      721 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
      781 atgttctttc ctgcggtacc gatcataatc agccatacca catttgtaga ggttttactt
      841 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
      901 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
      961 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     1021 gtatcttatc atgtctggat cataatcagc cataccacat ttgtagaggt tttacttgct
     1081 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     1141 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     1201 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     1261 tcttatcatg tctgctcgag gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg
     1321 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc
     1381 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag
     1441 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg
     1501 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg
     1561 tcgacggtat cgataagctt acgcgtgcta gcgcatgcag atctactagt cggccggata
     1621 aagatatcaa ctcgagatgg gatcggccat tgaacaagat ggattgcacg caggttctcc
     1681 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     1741 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     1801 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     1861 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     1921 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     1981 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     2041 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     2101 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     2161 caggctcaag gcgcgcatgc ccgacggcga tgatctcgtc gtgacccatg gcgatgcctg
     2221 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     2281 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     2341 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     2401 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga ggggatcaat tctctagagc
     2461 tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     2521 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     2581 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     2641 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     2701 ggcttctgag gcggaaagaa ccagctgggg gctagcaaag cctaggaaga cgtacgtata
     2761 cgcgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag
     2821 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
     2881 atccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt
     2941 atgtcgaggt aggcgtgtac ggtgggaagg ccgctctagc ccgggcggat cccccgggct
     3001 gcaggaattc gatatcaagc ttgccaccat gccagagcca gcgaagtctg ctcccgcccc
     3061 gaaaaagggc tccaagaagg cggtgactaa ggcgcagaag aaaggcggca agaagcgcaa
     3121 gcgcagccgc aaggagagct attccatcta tgtgtacaag gttctgaagc aggtccaccc
     3181 tgacaccggc atttcgtcca aggccatggg catcatgaat tcgtttgtga acgacatttt
     3241 cgagcgcatc gcaggtgagg cttcccgcct ggcgcattac aacaagcgct cgaccatcac
     3301 ctccagggag atccagacgg ccgtgcgcct gctgctgcct ggggagttgg ccaagcacgc
     3361 cgtgtccgag ggtactaagg ccatcaccaa gtacaccagc gctaaggatc cccgggtacc
     3421 ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     3481 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     3541 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     3601 ctggcccacc ctcgtgacca ccttcggcta cggcctgatg tgcttcgccc gctaccccga
     3661 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     3721 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     3781 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     3841 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     3901 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     3961 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     4021 cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga
     4081 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     4141 gtacaagtag gtcgacctcg agggggggcc cggtaccagg taagtgtacc caattcgccc
     4201 tatagtgagt cgtattacaa ttcactcgat cgcccttccc aacagttgcg cagcctgaat
     4261 ggcgaatgga gatccaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt
     4321 gtgtatttta gattcacagt cccaaggctc atttcaggcc cctcagtcct cacagtctgt
     4381 tcatgatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     4441 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     4501 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     4561 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct taacgcgtaa
     4621 attgtaagcg tctcatgaag acgacgcgaa attcagcatt ttcacaagcg ttatctttta
     4681 caaaaccgat ctcactctcc tttgatgcga atgccagcgt cagacatcat atgcagatac
     4741 tcacctgcat cctgaaccca ttgacctcca accccgtaat agcgatgcgt aatgatgtcg
     4801 atagttacta acgggtcttg ttcgattaac tgccgcagaa actcttccag gtcaccagtg
     4861 cagtgcttga taacaggagt cttcccagga tggcgaacaa caagaaactg gtttccgtct
     4921 tcacggactt cgttgctttc cagtttagca atacgcttac tcccatccga gataacacct
     4981 tcgtaatact cacgctgctc gttgagtttt gattttgctg tttcaagctc aacacgcagt
     5041 ttccctactg ttagcgcaat atcctcgttc tcctggtcgc ggcgtttgat gtattgctgg
     5101 tttctttccc gttcatccag cagttccagc acaatcgatg gtgttaccaa ttcatggaaa
     5161 aggtctgcgt caaatcccca gtcgtcatgc attgcctgct ctgccgcttc acgcagtgcc
     5221 tgagagttaa tttcgctcac ttcgaacctc tctgtttact gataagttcc agatcctcct
     5281 ggcaacttgc acaagtccga caaccctgaa cgaccaggcg tcttcgttca tctatcggat
     5341 cgccacactc acaacaatga gtggcagata tagcctggtg gttcaggcgg cgcattttta
     5401 ttgctgtgtt gcgctgtaat tcttctattt ctgatgctga atcaatgatg tctgccatct
     5461 ttcattaatc cctgaactgt tggttaatac gcttgagggt gaatgcgaat aataaaaaag
     5521 gagcctgtag ctccctgatg attttgcttt tcatgttcat cgttccttaa agacgccgtt
     5581 taacatgccg attgccaggc ttaaatgagt cggtgtgaat cccatcagcg ttaccgtttc
     5641 gcggtgcttc ttcagtacgc tacggcaaat gtcatcgacg tttttatccg gaaactgctg
     5701 tctggctttt tttgatttca gaattagcct gacgggcaat gctgcgaagg gcgttttcct
     5761 gctgaggtgt cattgaacaa gtcccatgtc ggcaagcata agcacacaga atatgaagcc
     5821 cgctgccaga aaaatgcatt ccgtggttgt catacctggt ttctctcatc tgcttctgct
     5881 ttcgccacca tcattccagc tttcctaggg acgtccatat gaatgtagtc ttatgcaata
     5941 cacttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa
     6001 aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg
     6061 caacagacag gtctgacatg gattggacga accactgaat tgcgcattgc agagataatt
     6121 gtatttaagt gcctagctcg atacaataaa cgccatttga ccattcacca cattggtgtg
     6181 caccctgcag atctagaatg ccagagccag cgaagtctgc tcccgccccg aaaaagggct
     6241 ccaagaaggc ggtgactaag gcgcagaaga aaggcggcaa gaagcgcaag cgcagccgca
     6301 aggagagcta ttccatctat gtgtacaagg ttctgaagca ggtccaccct gacaccggca
     6361 tttcgtccaa ggccatgggc atcatgaatt cgtttgtgaa cgacattttc gagcgcatcg
     6421 caggtgaggc ttcccgcctg gcgcattaca acaagcgctc gaccatcacc tccagggaga
     6481 tccagacggc cgtgcgcctg ctgctgcctg gggagttggc caagcacgcc gtgtccgagg
     6541 gtactaaggc catcaccaag tacaccagcg ctaaggatcc accggtcgcc accatggtga
     6601 gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca
     6661 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     6721 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct
     6781 gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg
     6841 acattcccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
     6901 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt
     6961 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
     7021 agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga
     7081 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca
     7141 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca
     7201 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg
     7261 agggccgcca ctccaccggc ggcatggacg agctgtacaa gtcttaagcg actctagatc
     7321 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     7381 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     7441 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     7501 ctgcattcta gttgtggttt gtccaaactc atcaatgtat ctcatatgtg tatccgctca
     7561 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     7621 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     7681 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     7741 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     7801 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     7861 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     7921 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     7981 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     8041 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     8101 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     8161 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     8221 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     8281 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     8341 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     8401 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     8461 agcattggta actgtcagac caagtttact catatatact ttagatt
//
