LOCUS       sequence-355475-        8332 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous 
            gene tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8332)
  AUTHORS   Paterou A, Saez Conde J, Tyc J, Sunter JD, Vaughan S, Gull K, Dean S
  TITLE     A comprehensive toolkit for protein localization and functional 
            analysis in trypanosomatids.
  JOURNAL   Open Biol. 2025 Apr;15(4):240361. doi: 10.1098/rsob.240361. Epub 
            2025 Apr 2.
  PUBMED    40169015
REFERENCE   2  (bases 1 to 8332)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Open 
            Biol."; date: "2025-04"; volume: "15(4):240361. doi"; pages: " 
            10.1098/rsob.240361. Epub 2025 Apr 2"
COMMENT     Sequence Label: pPOTv7-g418-3HaloTag
FEATURES             Location/Qualifiers
     source          1..8332
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             171..965
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             1383..2270
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
     CDS             2280..3167
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
     CDS             3180..4067
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
     CDS             4470..5264
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(5457..5475)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5458..5475)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5493..5509)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5493..5509)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5506..5528)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5517..5533
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5541..5547,5548..5565,5566..5571))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5586..5607
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5724..5741)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5895..6483)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5975..5994)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6654..7445,7446..7514))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVIIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7277..7296
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7515..7619)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7697..8152)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7834..7855)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8046..8065
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8278..8300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8292..8309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8293..8309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(8316..8332,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(8316..8332,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgtata atgcagacct gctgcaagct aggggcacag caaggtcttc
       61 tgaaattcat gttttttttt tttttactct gcattgcagt ctccgctctt atttagtttt
      121 gctttacgta aggtctcgtt gctgccataa aataagctct agtggaattc atgattgaac
      181 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
      241 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
      301 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
      361 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
      421 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
      481 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
      541 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
      601 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
      661 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
      721 tcgtcgtgac ccacggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
      781 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
      841 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
      901 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
      961 tctgaccatg gtgcccattt agttggcctt tcccttgtct cgtgtctttt ccgtggaaag
     1021 gttcccggag taatctgatg gcacagcagg gaggtgcgcc tgcaggttgg ttaggaaggg
     1081 gggatgatgt aaaagaagaa aatgggggga ttcgagcccc acgcgttgct caagctgtgt
     1141 agcgcaggcg tttccttaca tatttctcta acaggcacgg aagcctaaca aatacacttg
     1201 gcttattttt ttgccccctc atgtcttgta caaatatttg cgatagctta gctatcagcc
     1261 acattaatca aacaagtata ccaacaagcc cgaaaacaga aactcaactg caacgggtac
     1321 ctatgggttc tggtagtggt tccggttccg gttctaagct tggtgggtct ggaagcggta
     1381 gtgccgaaat aggcacaggc ttcccctttg atccgcatta cgtggaagtt ctgggggagc
     1441 gtatgcacta cgtcgacgtt ggacccaggg atggtacgcc ggttcttttc ctgcacggga
     1501 accccacgtc gagctacgtg tggcgaaaca tcataccgca cgtggctccc acgcaccgat
     1561 gtattgcgcc agatcttata ggcatgggga agtccgacaa acccgatctt ggttacttct
     1621 tcgacgacca tgtgcgcttc atggatgctt ttatcgaggc acttggattg gaggaggttg
     1681 ttttggtaat acacgactgg ggctcagccc ttggctttca ttgggcgaag cgtaatccag
     1741 agcgggttaa aggcatcgct tttatggagt tcataaggcc catcccgacg tgggatgagt
     1801 ggccagagtt tgccagggag accttccaag cctttcgcac gaccgacgtc ggtcgcaaat
     1861 tgataataga ccaaaacgtg tttattgagg gaaccctgcc tatgggagta gtcaggcccc
     1921 tcactgaagt ggaaatggac cactataggg agccattttt gaatccggtt gatcgggaac
     1981 ctctttggcg atttccaaat gaacttccta ttgcaggaga gcccgccaac atcgttgctc
     2041 tggtcgagga atatatggat tggctccatc agtcaccggt cccgaaactt ctgttttggg
     2101 ggaccccagg agttttgata ccccctgccg aggcggcccg ccttgccaaa tcgcttccca
     2161 attgcaaagc agttgacatc ggaccaggtc tcaacctgct ccaggaagat aatccagatt
     2221 tgatcggctc ggaaatcgct cgctggctgt cgacactgga gatatctggt tcgggctcgg
     2281 ctgagatagg tactgggttt ccgttcgacc ctcactacgt agaagttctt ggagagcgga
     2341 tgcactatgt cgatgtgggg cctcgcgatg gcacccccgt acttttcctc cacggcaacc
     2401 ccacgtcgag ctatgtctgg aggaacataa taccacacgt ggctccaacg cataggtgta
     2461 tagctcccga ccttatagga atgggcaaaa gcgacaaacc cgaccttgga tacttcttcg
     2521 atgatcacgt acgcttcatg gacgctttca tcgaagccct gggcttggag gaggttgtct
     2581 tggttattca tgattgggga tcagctcttg ggtttcactg ggctaaacgc aaccctgaac
     2641 gggtaaaagg catcgcgttc atggagttta tacgtcccat accaacctgg gacgaatggc
     2701 cggagttcgc acgtgagacc ttccaggcct ttcgcactac cgatgtaggc aggaagctga
     2761 tcattgacca aaacgtcttt attgaaggca ccctgcctat gggagttgtc aggcctctta
     2821 ctgaggtcga aatggaccat taccgggaac cgtttctgaa tccggtggat cgggaaccac
     2881 tttggaggtt ccccaatgaa ctcccgattg ccggcgagcc agccaacatc gttgcgctcg
     2941 ttgaagaata catggactgg ctgcaccaga gtccggtgcc gaaactcttg ttttggggca
     3001 cccctggcgt tctgatccca ccggccgagg cggcccgact cgccaagtca ttgcctaact
     3061 gtaaagcggt tgatatcggc cctggactta atcttctgca agaagacaac ccagacctga
     3121 tcggctccga gatcgcacgg tggttgtcta ctcttgaaat ttccggttcg cccgggtcgg
     3181 ctgagattgg tacggggttt ccttttgacc cgcactacgt tgaggtgttg ggtgaacgta
     3241 tgcattatgt tgatgttggt ccccgtgatg gtacgcccgt gctgtttttg catggtaacc
     3301 ccacgtcaag ctatgtgtgg aggaacatta ttccccacgt ggcacctact catcgctgta
     3361 ttgctccaga ccttattggt atgggtaagt cagataaacc ggatcttggt tacttctttg
     3421 acgatcacgt gcgttttatg gatgcattta ttgaagcgct tggccttgaa gaagttgtgt
     3481 tagtgattca cgattggggt tctgccttgg gatttcattg ggctaaacgt aatcccgaaa
     3541 gagttaaagg tattgcattc atggagttca ttcgtcctat tcctacgtgg gatgagtggc
     3601 cagagtttgc gcgcgagacg tttcaagcgt ttcgtacaac ggacgtgggt cgtaagctga
     3661 ttattgatca aaatgttttt attgagggta cgctgccgat gggtgttgtg cgtcccctga
     3721 ctgaagtgga gatggatcac taccgtgagc catttttaaa tccagtggac cgtgagccat
     3781 tgtggcgctt ccctaacgag ctgcccattg ctggtgaacc ggctaacatt gtggcgcttg
     3841 tggaggagta tatggattgg cttcatcaat cacccgtgcc taagttgctt ttttggggta
     3901 cgccaggtgt gcttattcca cctgctgaag cagcacgctt ggctaaatca cttccaaact
     3961 gcaaagcagt tgatattggt ccgggtctga acctgttgca agaagataat cccgatctta
     4021 ttggttccga gattgcccgt tggctttcga cacttgaaat ttctggtgga tccggatcag
     4081 gatctggatc aggatcgggt agttaagagc tccgctgcgc ttaaatgtct ttcattataa
     4141 tcaatgtata acctttatgt agtatttcaa tctatgccgc tgtgtacgtg cactgcggtg
     4201 cctatccttc ggcattagag agtcactgtt tgtgtagatc gtagctgcat gtctgacatc
     4261 tggttcatta gtgccgtttt gagtctgcac tgttgtgcaa cttgatattc atcactgcac
     4321 accaaccgtg tgtttcctcc tttgggtgca ccctttaatc cttgtcttct ccttttttgt
     4381 ctctttcccc ctcgaaaagg tgtcaaacta ctgccgcata aactacggtt atcccaaatt
     4441 taagagaaag caataaagca tcagctagca tgattgaaca agatggattg cacgcaggtt
     4501 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     4561 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     4621 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     4681 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     4741 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     4801 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     4861 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     4921 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     4981 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc cacggcgatg
     5041 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     5101 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     5161 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     5221 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgactcgag tgcccattta
     5281 gttggctttt cccttgtctc gtgtcttttc cgtggaaagg ttcccggagt aatctgatgg
     5341 cacagcaggg aggtgcgcct gcaggttggt taggaagggg ggatgatgta aaagaagaaa
     5401 atggggggat tcgagccccg cacaggtctc tcaaattgga tgcatagctt gagtattcta
     5461 tagtgtcacc taaatagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     5521 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     5581 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     5641 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     5701 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     5761 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     5821 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     5881 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     5941 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     6001 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     6061 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     6121 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     6181 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     6241 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     6301 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     6361 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     6421 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     6481 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     6541 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     6601 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     6661 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     6721 tgactccccg tcgtgtagat aattacgata cgggagggct taccatctgg ccccagtgct
     6781 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     6841 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     6901 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     6961 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     7021 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     7081 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     7141 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     7201 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     7261 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     7321 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     7381 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     7441 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     7501 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     7561 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     7621 acatttcccc gaaaagtgcc acctgatgcg gtgtgaaata ccgcacagat gcgtaaggag
     7681 aaaataccgc atcaggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     7741 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     7801 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     7861 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     7921 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     7981 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     8041 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     8101 ctgcgcgtaa ccaccacacc cgccgcactt aatgcgccgc tacagggcgc gtccattcgc
     8161 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     8221 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     8281 agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta ta
//
