LOCUS       sequence-355477-        8805 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses rTetR-KRAB(human) for gene silencing, integration by 
            Piggybac.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8805)
  AUTHORS   Lensch S, Herschl MH, Ludwig CH, Sinha J, Hinks MM, Mukund A, 
            Fujimori T, Bintu L
  TITLE     Dynamic spreading of chromatin-mediated gene silencing and 
            reactivation between neighboring genes in single cells.
  JOURNAL   Elife. 2022 Jun 9;11. pii: 75115. doi: 10.7554/eLife.75115.
  PUBMED    35678392
REFERENCE   2  (bases 1 to 8805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-06-9"; volume: "11. pii: 75115. doi"; pages: " 
            10.7554/eLife.75115"
COMMENT     Sequence Label: pLB62_PB_pGK-H2B-mIFP-T2A-rTetR-humanKRAB-zeo
FEATURES             Location/Qualifiers
     source          1..8805
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        352..851
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(387..410)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     784..803
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             881..1258
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             1277..2236
                     /codon_start=1
                     /product="naturally monomeric infrared fluorescent protein 
                     derived from a truncated bacteriophytochrome (Yu et al., 
                     2015)"
                     /label=mIFP
                     /translation="MSVPLTTSAFGHAFLANCEREQIHLAGSIQPHGILLAVKEPDNVV
                     IQASINAAEFLNTNSVVGRPLRDLGGDLPLQILPHLNGPLHLAPMTLRCTVGSPPRRVD
                     CTIHRPSNGGLIVELEPATKTTNIAPALDGAFHRITSSSSLMGLCDETATIIREITGYD
                     RVMVVRFDEEGNGEILSERRRADLEAFLGNRYPASTIPQIARRLYEHNRVRLLVDVNYT
                     PVPLQPRISPLNGRDLDMSLSCLRSMSPIHQKYMQDMGVGATLVCSLMVSGRLWGLIAC
                     HHYEPRFVPFHIRAAGEALAETCAIRIATLESFAQSQSK"
     CDS             2237..2290
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2960..3145
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
     primer_bind     complement(3165..3182)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3171..3378
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(3411..3431)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3416..3745
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3596..3731
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3658..3677
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3793..3840
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3859..4233
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     primer_bind     complement(4293..4313)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    4355..4476
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4392..4411)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4446..4465
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   5239..5273
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5764..5780)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5764..5780)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5777..5799)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5788..5804
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5812..5818,5819..5836,5837..5842))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5857..5878
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5995..6012)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6166..6754)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6246..6265)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6925..7716,7717..7785))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7548..7567
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7786..7890)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7916..8371)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8053..8074)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8265..8284
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8498..8520
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8512..8529
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8513..8529
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   8659..8721
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
       61 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
      121 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
      181 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
      241 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
      301 cacctggggg gatacgggga aaaggcctcc acggccacta gtaattctac cgggtagggg
      361 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc
      421 gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg
      481 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc
      541 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta
      601 ggctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg
      661 gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg
      721 ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc
      781 cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc
      841 cgggcctttc gacctgcagc ccaagcttgc tagcgccacc atgccagagc cagcgaagtc
      901 tgctcccgcc ccgaaaaagg gctccaagaa ggcggtgact aaggcgcaga agaaaggcgg
      961 caagaagcgc aagcgcagcc gcaaggagag ctattccatc tatgtgtaca aggttctgaa
     1021 gcaggtccac cctgacaccg gcatttcgtc caaggccatg ggcatcatga attcgtttgt
     1081 gaacgacatt ttcgagcgca tcgcaggtga ggcttcccgc ctggcgcatt acaacaagcg
     1141 ctcgaccatc acctccaggg agatccagac ggccgtgcgc ctgctgctgc ctggggagtt
     1201 ggccaagcac gccgtgtccg agggtactaa ggccatcacc aagtacacca gcgctaagga
     1261 tccaccggtc gccaccatgt cggtaccgct gactacctca gcattcggcc acgcgtttct
     1321 ggctaactgt gaacgcgagc agatccacct ggcgggctcc attcagccgc acggtatcct
     1381 gctggctgtg aaagagccgg acaacgtggt gatccaggct tctattaacg ctgcggagtt
     1441 cctgaacacc aactctgttg ttggccgtcc gctgcgtgac ctgggcggcg atctgccttt
     1501 gcagatcctg ccgcacctga acggcccgct gcacctggct ccgatgaccc tgcgttgtac
     1561 cgtgggttct ccgccgcgtc gtgtggactg taccattcat cgtccgtcta acggcggcct
     1621 gatcgtagaa ctggaaccag caaccaagac cactaacatt gcgccggctc tggacggtgc
     1681 gtttcatcgt atcacttctt catcctccct gatgggcctg tgtgacgaaa ccgcgactat
     1741 tatccgtgag attactggct acgaccgtgt gatggtagta cgtttcgatg aagagggtaa
     1801 tggcgaaatt ctgtccgaac gtcgtcgtgc ggacctggaa gcgttcctgg gtaaccgcta
     1861 cccggcgtct actattccgc agatcgctcg tcgcctgtac gaacataacc gtgttcgcct
     1921 gctggtagat gtgaactata ctccggttcc gctacagccg cgcatcagcc cgctgaacgg
     1981 tcgtgatctg gatatgtccc tgtcttgcct gcgctctatg tccccgatcc accagaaata
     2041 catgcaggac atgggcgttg gcgcgaccct ggtttgctct ctgatggtgt ctggtcgtct
     2101 gtggggtctg atcgcttgcc accactacga accgcgcttc gttccgttcc acattcgcgc
     2161 tgctggcgaa gcgctggcgg aaacttgtgc gatccgcatc gcgacgctgg agagctttgc
     2221 acagtctcag tccaaagagg gcagggggag cctcctaact tgcggagacg tcgaggagaa
     2281 tcctggccca tctagactgg acaagagcaa agtcataaac ggagctctgg aattactcaa
     2341 tggtgtcggt atcgaaggcc tgacgacaag gaaactcgct caaaagctgg gagttgagca
     2401 gcctaccctg tactggcacg tgaagaacaa gcgggccctg ctcgatgccc tgccaatcga
     2461 gatgctggac aggcatcata cccacttctg ccccctggaa ggcgagtcat ggcaagactt
     2521 tctgcggaac aacgccaagt cataccgctg tgctctcctc tcacatcgcg acggggctaa
     2581 agtgcatctc ggcacccgcc caacagagaa acagtacgaa accctggaaa atcagctcgc
     2641 gttcctgtgt cagcaaggct tctccctgga gaacgcactg tacgctctgt ccgccgtggg
     2701 ccactttaca ctgggctgcg tattggagga acaggagcat caagtagcaa aagaggaaag
     2761 agagacacct accaccgatt ctatgccccc acttctgaga caagcaattg agctgttcga
     2821 ccggcaggga gccgaacctg ccttcctttt cggcctggaa ctaatcatat gtggcctgga
     2881 gaaacagcta aagtgcgaaa gcggcggggg tagcggcagc ggtagcgcat gcgatgctaa
     2941 gtcactaact gcctggtccc ggacactggt gaccttcaag gatgtatttg tggacttcac
     3001 cagggaggag tggaagctgc tggacactgc tcagcagatc gtgtacagaa atgtgatgct
     3061 ggagaactat aagaacctgg tttccttggg ttatcagctt actaagccag atgtgatcct
     3121 ccggttggag aagggagaag agccctaatt cgaatttaaa tcggcctcga ctgtgccttc
     3181 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     3241 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     3301 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     3361 tagcaggcat gctggggacg cggccgcaat ttaacgcgaa ttaattctgt ggaatgtgtg
     3421 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     3481 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     3541 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     3601 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     3661 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     3721 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     3781 tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac
     3841 aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc
     3901 gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg
     3961 gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag
     4021 gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg
     4081 tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg
     4141 accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac
     4201 tgcgtgcact tcgtggccga ggagcaggac tgagtcgaca atcaacctct ggattacaaa
     4261 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     4321 gctgctttaa tgcctttgta tcatgcgtta actaaacttg tttattgcag cttataatgg
     4381 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     4441 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggaatt gactcaaatg
     4501 atgtcaatta gtctatcaga agctcatctg gtctcccttc cgggggacaa gacatccctg
     4561 tttaatattt aaacagcagt gttcccaaac tgggttctta tatcccttgc tctggtcaac
     4621 caggttgcag ggtttcctgt cctcacagga acgaagtccc taaagaaaca gtggcagcca
     4681 ggtttagccc cggaattgac tggattcctt ttttagggcc cattggtatg gctttttccc
     4741 cgtatccccc caggtgtctg caggctcaaa gagcagcgag aagcgttcag aggaaagcga
     4801 tcccgtgcca ccttccccgt gcccgggctg tccccgcacg ctgccggctc ggggatgcgg
     4861 ggggagcgcc ggaccggagc ggagccccgg gcggctcgct gctgccccct agcgggggag
     4921 ggacgtaatt acatccctgg gggctttggg ggggggctgt ccctgatatc tataacaaga
     4981 aaatatatat ataataagtt atcacgtaag tagaacatga aataacaata taattatcgt
     5041 atgagttaaa tcttaaaagt cacgtaaaag ataatcatgc gtcattttga ctcacgcggt
     5101 cgttatagtt caaaatcagt gacacttacc gcattgacaa gcacgcctca cgggagctcc
     5161 aagcggcgac tgagatgtcc taaatgcaca gcgacggatt cgcgctattt agaaagagag
     5221 agcaatattt caagaatgca tgcgtcaatt ttacgcagac tatctttcta gggttaatct
     5281 agctgcatca ggatcatatc gtcgggtctt ttttccggct cagtcatcgc ccaagctggc
     5341 gctatctggg catcggggag gaagaagccc gtgccttttc ccgcgaggtt gaagcggcat
     5401 ggaaagagtt tgccgaggat gactgctgct gcattgacgt tgagcgaaaa cgcacgttta
     5461 ccatgatgat tcgggaaggt gtggccatgc acgcctttaa cggtgaactg ttcgttcagg
     5521 ccacctggga taccagttcg tcgcggcttt tccggacaca gttccggatg gtcagcccga
     5581 agcgcatcag caacccgaac aataccggcg acagccggaa ctgccgtgcc ggtgtgcaga
     5641 ttaatgacag cggtgcggcg ctgggatatt acgtcagcga ggacgggtat cctggctgga
     5701 tgccgcagaa atggacatgg ataccccgtg agttacccgg cgggcgcgct tggcgtaatc
     5761 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     5821 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     5881 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     5941 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     6001 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     6061 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     6121 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     6181 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     6241 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     6301 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     6361 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     6421 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     6481 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     6541 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     6601 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     6661 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     6721 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     6781 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     6841 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     6901 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     6961 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     7021 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     7081 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     7141 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     7201 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     7261 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     7321 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     7381 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     7441 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     7501 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     7561 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     7621 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     7681 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     7741 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     7801 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     7861 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctaaatt
     7921 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     7981 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     8041 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     8101 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     8161 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     8221 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     8281 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     8341 gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct gcgcaactgt
     8401 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     8461 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     8521 acggccagtg agcgcgcctc gttcattcac gtttttgaac ccgtggagga cgggcagact
     8581 cgcggtgcaa atgtgtttta cagcgtgatg gagcagatga agatgctcga cacgctgcag
     8641 aacacgcagc tagattaacc ctagaaagat aatcatattg tgacgtacgt taaagataat
     8701 catgtgtaaa attgacgcat gtgttttatc ggtctgtata tcgaggttta tttattaatt
     8761 tgaatagata ttaagtttta ttatatttac acttacatac taata
//
