LOCUS       sequence-355528-        9771 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual fluorescent reporter construct with single HS4 insulator, 
            upstream TRE (Tetracycline Response Element) recruitment site, 
            phiC31 attB for integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9771)
  AUTHORS   Lensch S, Herschl MH, Ludwig CH, Sinha J, Hinks MM, Mukund A, 
            Fujimori T, Bintu L
  TITLE     Dynamic spreading of chromatin-mediated gene silencing and 
            reactivation between neighboring genes in single cells.
  JOURNAL   Elife. 2022 Jun 9;11. pii: 75115. doi: 10.7554/eLife.75115.
  PUBMED    35678392
REFERENCE   2  (bases 1 to 9771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-06-9"; volume: "11. pii: 75115. doi"; pages: " 
            10.7554/eLife.75115"
COMMENT     Sequence Label: 
            pMH002_phiC31_neo-5xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry
FEATURES             Location/Qualifiers
     source          1..9771
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    16..85
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             351..1154
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(414..433)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1024..1043
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(1186..1203)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1192..1416
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    1489..1507
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1525..1543
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1561..1579
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1596..1614
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1632..1650
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     intron          1895..2833
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2790..2810
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        2866..2884
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             2975..3352
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      3371..3380
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3377..3379,3380..3382,3383..4093)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3422..3443)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3683..3702)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4030..4051
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4144..4163)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4145..4163)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    4437..4558
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        5900..6162
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     CDS             6176..6553
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      6566..6575
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6572..7285
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYKS"
     primer_bind     complement(6717..6735)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6985..7004
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    7401..7482
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        7525..7588
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7589..7657,7658..8449)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7807..7826)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8620..9208
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9109..9128
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    9392..9526
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    9629..9750
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 gatgtaggtc acggtctcga agccgcggtg cgggtgccag ggcgtgccct tgggctcccc
       61 gggcgcgtac tccacctcac ccatctggtc catcatgatg aacgggtcga ggtggcggta
      121 gttgatcccg gcgaacgcgc ggcgcaccgg gaagccctcg ccctcgaaac cgctgggcgc
      181 ggtggtcacg gtgagcacgg gacgtgcgac ggcgtcggcg ggtgcggata cgcggggcag
      241 cgtcagcggg ttctcgacgg tcacggcggg catgtcgacg gtatcgataa gcttacgcgt
      301 gctagcgcat gcagatctac tagtcggccg gataaagata tcaactcgag atgggatcgg
      361 ccattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
      421 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
      481 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
      541 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
      601 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
      661 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
      721 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
      781 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
      841 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
      901 gcgatgatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
      961 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     1021 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     1081 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     1141 acgagttctt ctgaggggat caattctcta gagctcgctg atcagcctcg actgtgcctt
     1201 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     1261 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     1321 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     1381 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     1441 gggggctagc aaagcctagg aagacgtacg tatacgcgtc gagtttactc cctatcagtg
     1501 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac
     1561 tccctatcag tgatagagaa cgtatgtcga gtttatccct atcagtgata gagaacgtat
     1621 gtcgagttta ctccctatca gtgatagaga acgtatgtcg aggtaggcgt gtacggtggg
     1681 aatgcatgag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     1741 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     1801 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     1861 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     1921 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
     1981 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     2041 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     2101 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     2161 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     2221 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     2281 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     2341 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     2401 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     2461 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     2521 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     2581 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     2641 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     2701 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     2761 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     2821 tcttccattt caggtgtcgt gagctagcga ttacgccaag ctcgaaatta accctcacta
     2881 aagggaacaa aagctggagc tccaccgcgg tggcggccgc tctagcccgg gcggatcccc
     2941 cgggctgcag gaattcgata tcaagcttgc caccatgcca gagccagcga agtctgctcc
     3001 cgccccgaaa aagggctcca agaaggcggt gactaaggcg cagaagaaag gcggcaagaa
     3061 gcgcaagcgc agccgcaagg agagctattc catctatgtg tacaaggttc tgaagcaggt
     3121 ccaccctgac accggcattt cgtccaaggc catgggcatc atgaattcgt ttgtgaacga
     3181 cattttcgag cgcatcgcag gtgaggcttc ccgcctggcg cattacaaca agcgctcgac
     3241 catcacctcc agggagatcc agacggccgt gcgcctgctg ctgcctgggg agttggccaa
     3301 gcacgccgtg tccgagggta ctaaggccat caccaagtac accagcgcta aggatccccg
     3361 ggtaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     3421 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     3481 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     3541 cgtgccctgg cccaccctcg tgaccacctt cggctacggc ctgatgtgct tcgcccgcta
     3601 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     3661 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     3721 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     3781 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     3841 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     3901 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     3961 gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc ccaacgagaa
     4021 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     4081 cgagctgtac aagtaggtcg acctcgaggg ggggcccggt accaggtaag tgtacccaat
     4141 tcgccctata gtgagtcgta ttacaattca ctcgatcgcc cttcccaaca gttgcgcagc
     4201 ctgaatggcg aatggagatc caatttttaa gtgtataatg tgttaaacta ctgattctaa
     4261 ttgtttgtgt attttagatt cacagtccca aggctcattt caggcccctc agtcctcaca
     4321 gtctgttcat gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     4381 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     4441 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     4501 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaac
     4561 gcgtaaattg taagcgtctc agtaaattgt aagcgtgaga tccgtttcga taagctctag
     4621 agggacagcc cccccccaaa gcccccaggg atgtaattac gtccctcccc cgctaggggg
     4681 cagcagcgag ccgcccgggg ctccgctccg gtccggcgct ccccccgcat ccccgagccg
     4741 gcagcgtgcg gggacagccc gggcacgggg aaggtggcac gggatcgctt tcctctgaac
     4801 gcttctcgct gctctttgag cctgcagaca cctgggggga tacggggaaa aagctttagg
     4861 ctgaaagaga gatttagaat gacagaatca tagaacggcc tgggttgcaa aggagcacag
     4921 tgctcatcca gatccaaccc cctgctatgt gcagggtcat caaccagcag cccaggctgc
     4981 ccagagccac atccagcctg gccttgaatg cctgcaggga tggggcatcc acagcctcct
     5041 tgggcaacct gttcagtgcg tcaccaccct ctgggggaaa aactgcctcc tcatatccaa
     5101 cccaaacctc ccctgtctca gtgtaaagcc attccccctt gtcctatcaa gggggagttt
     5161 gctgtgacat tgttggtctg gggtgacaca tgtttgccaa ttcagtgcat cacggagagg
     5221 cagatcttgg ggataaggaa gtgcaggaca gcatggacgt gggacatgca ggtgttgagg
     5281 gctctgggac actctccaag tcacagcgtt cagaacagcc ttaaggataa gaagatagga
     5341 tagaaggaca aagagcaagt taaaacccag catggagagg agcacaaaaa ggccacagac
     5401 actgctggtc cctgtgtctg agcctgcatg tttgatggtg tctggatgca agcagaaggg
     5461 gtggaagagc ttgcctggag agatacagct gggtcagtag gactgggaca ggcagctgga
     5521 gaattgccat gtagatgttc atacaatcgt caaatcatga aggctggaaa agccctccaa
     5581 gatccccaag accaacccca acccacccac cgtgcccact ggccatgtcc ctcagtgcca
     5641 catccccaca gttcttcatc acctccaggg acggtgaccc ccccacctcc gtgggcagct
     5701 gtgccactgc agcaccgctc tttggagaag gtaaatcttg ctaaatccag cccgaccctc
     5761 ccctggcaca acgtaaggcc attatctctc atccaactcc aggacggagt cagtgaggat
     5821 ggggctctag cggggggatc aaacggccgg cctctagagt cgagatgaca ttgattattg
     5881 acctagggac gtccatatga atgtagtctt atgcaataca cttgtagtct tgcaacatgg
     5941 taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg
     6001 tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacaggt ctgacatgga
     6061 ttggacgaac cactgaattg cgcattgcag agataattgt atttaagtgc ctagctcgat
     6121 acaataaacg ccatttgacc attcaccaca ttggtgtgca ccctgcagat ctagaatgcc
     6181 agagccagcg aagtctgctc ccgccccgaa aaagggctcc aagaaggcgg tgactaaggc
     6241 gcagaagaaa ggcggcaaga agcgcaagcg cagccgcaag gagagctatt ccatctatgt
     6301 gtacaaggtt ctgaagcagg tccaccctga caccggcatt tcgtccaagg ccatgggcat
     6361 catgaattcg tttgtgaacg acattttcga gcgcatcgca ggtgaggctt cccgcctggc
     6421 gcattacaac aagcgctcga ccatcacctc cagggagatc cagacggccg tgcgcctgct
     6481 gctgcctggg gagttggcca agcacgccgt gtccgagggt actaaggcca tcaccaagta
     6541 caccagcgct aaggatccac cggtcgccac catggtgagc aagggcgagg aggataacat
     6601 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
     6661 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     6721 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
     6781 catgtacggc tccaaggcct acgtgaagca ccccgccgac attcccgact acttgaagct
     6841 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
     6901 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
     6961 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     7021 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
     7081 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
     7141 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
     7201 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
     7261 catggacgag ctgtacaagt cttaagcgac tctagatcat aatcagccat accacatttg
     7321 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     7381 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     7441 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     7501 ccaaactcat caatgtatct catatgtgta tccgctcatg agacaataac cctgataaat
     7561 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     7621 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     7681 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     7741 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     7801 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     7861 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     7921 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     7981 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     8041 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     8101 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     8161 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     8221 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     8281 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     8341 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     8401 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     8461 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     8521 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     8581 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     8641 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     8701 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     8761 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     8821 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     8881 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     8941 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     9001 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     9061 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     9121 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     9181 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     9241 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg gtaccgatca taatcagcca
     9301 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     9361 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     9421 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     9481 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcataa tcagccatac
     9541 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     9601 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     9661 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     9721 tggtttgtcc aaactcatca atgtatctta tcatgtctgc tcgaggtcga c
//
