LOCUS       sequence-355531-        2468 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-hC11
FEATURES             Location/Qualifiers
     source          1..2468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(388..393,394..410,411..416))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(543..1131)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(623..642)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1404..1420
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1404..1420
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1425..1444
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1425..1443
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(join(1618..2468,1..340))
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     2437..2456
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagagaatc cacaggacgg
       61 gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg
      121 ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca
      181 acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat
      241 cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga
      301 cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt
      361 agcaatttaa ctgtgataaa ctaccgcatt aaagctaatc gatgataagc tgtcaaacat
      421 gagaattaat tcatgttctt tcctgcgtta tcccctgatt ctgtggataa cgcaggaaag
      481 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      541 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      601 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      661 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      721 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      781 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      841 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
      901 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
      961 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     1021 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     1081 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     1141 ttgatctttt ctacggggtc tgacgctcag tggaacgacg cgtaactcac gttaagggat
     1201 tttggtcatg ggtggctcga ccagatatgg tctcacgcgg ccaacgccgg atcagggcga
     1261 gataactggg caacaatgct ctccagcgcc ggcctgccgc cgtcatgtga ccgagaccag
     1321 ctttggtcta gaggaggcat gatgatatat ttttatcttg tgcaatgtaa catcagagat
     1381 tttgagacac gggccagagc tgccaggaaa cagctatgac catgtaatac gactcactat
     1441 aggggatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg
     1501 acatactgtt cttccccgat caagggttgg tttgcgcatt cacagttctc cgcaagaatt
     1561 gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca
     1621 ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag
     1681 ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc
     1741 cgtgacgatc agcggtccaa tgatcgaagt taggctggta agagccgcga gcgatccttg
     1801 aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat
     1861 cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc
     1921 gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt
     1981 ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat
     2041 tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc
     2101 gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt
     2161 cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa
     2221 ggctctcaag ggcatgggtc ggcgctctcc cttatgcgac tcctgcatta ggaagcagcc
     2281 cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gtaaggagat
     2341 ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct
     2401 catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc
     2461 agcaaccg
//
