LOCUS       sequence-355534-        8595 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Punc-4::UNC-9-UrSL-wCherry unc-54 3' UTR C.elegans neural expression
            of UNC-9-wCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8595)
  AUTHORS   Kawano T, Po MD, Gao S, Leung G, Ryu WS, Zhen M
  TITLE     An imbalancing act: gap junctions reduce the backward motor circuit 
            activity to bias C. elegans for forward locomotion.
  JOURNAL   Neuron. 2011 Nov 17;72(4):572-86. doi: 10.1016/j.neuron.2011.09.005.
  PUBMED    22099460
REFERENCE   2  (bases 1 to 8595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2011.09"; journalName: "Neuron"; date: 
            "2011-11-17- 17"; volume: "72"; issue: "4"; pages: "572-86"
COMMENT     Sequence Label: pJH2141
FEATURES             Location/Qualifiers
     source          1..8595
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     99..118
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     352..369
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    486..507
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(522..527,528..545,546..552)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    560..576
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     565..587
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     584..600
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     584..600
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6764..6786)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6886..6905
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6983..7001)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7069..7173
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7174..7242,7243..8034)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7392..7411)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8205..8595,1..198)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
       61 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
      121 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
      181 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
      241 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
      301 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
      361 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
      421 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
      481 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
      541 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
      601 catgattacg ccaagctgta agtttaaaca tgatcttact aactaactat tctcatttaa
      661 attttcagag cttaaaaatg gctgaaatca ctcacaacga tggatacgct aacaacttgg
      721 aaatgaaata agcttgggct gcagcatgcg gccgcttccc caaattggaa cagtgaaata
      781 ctcaatcaat cactctggcg gttttctcca caaagaacac gaaattggaa aagggaaaag
      841 attgatttgt ttgaaggcgt gaggaaatcg aaaaaaaaat gtttcaaaac aaatgaaata
      901 ttttaatgaa taaacccagg aatgccacct tttggtgccc gcctacaaaa aagtcaactg
      961 ttcttaaact cctacatagt tgccctctct aacgaatgtt gcttttttta aattttcatt
     1021 gaatttgatt atcgtctgta tgcgcctact ctatcagtgg aaaacatgcc catttcccac
     1081 aaatttactc gtttggagct caaaatgtta aatggggcat catggaattg gcatctcaca
     1141 gaaagtggcg gatgagttat atgtaataat aatggtggct gaagtggaaa ccgcaaaaaa
     1201 aggatgtgta tttgagattt tgtaacggaa tttgaatcag atacacaaaa tgagagaaat
     1261 ttatagattc tttaaaatcc acctgaagtc ggcaaatttt tgaaaggtct gttcgttcag
     1321 tttttcataa gttttatgaa ttttgattta ctactgaagt tcgagaaaag ccctttaacc
     1381 ataccaataa gaaattatgt taactttaca agaatattaa caaaaaatta ttaaaaaact
     1441 gtgttcccct ggcattatga tatttccacc gtcagttctc tattaaaact tcaactctac
     1501 acagtttcaa ctctcaaatg agctgtccat cttttgcctt caggaaatta tgctaatctg
     1561 ctgatttcgc gcactttcac cacactattt ccaaatagaa ggaggggtgg tgcaacacac
     1621 gagacactgc aaaaaggttc agtgaagatt tatctacttg tgagatgaga gcaggagggg
     1681 tgggagaagt gtgatgattg acgggatgac aagaacagtg atgtggtgtc gaaatggaag
     1741 atgcgctaat ttgtggggtt ttatgaaatt tgaataatta catttataac atctactagt
     1801 taggcgtctg tgtaggtatt gcctgcctgc ctacaaggca tatgtacgag gcaggttaaa
     1861 cctcctagaa agctacggta ctttcaaacc acataacatt tatactggtg tcttttcgcg
     1921 tcttcttctt gaacatgtag accctcaaaa agctaagttt tacactgtgt agtttgcttt
     1981 gaaatctaca aaattggagg cacgccttct ggactttgcc gacacgcata ctttctacct
     2041 accaactgcc tacttccaca gtctagcgtg aactccaata ccattttaaa aattgtttta
     2101 tctcaaattt tcaattttta aatccaattt cccagttcta aaaaaaaaat ttttttttta
     2161 atttaaaaac tctcatcctt tttaatgctc gttcccattc ggcaagacgt gtaccaaaca
     2221 caaaacaaat tgactggtga tccatctcga atttctcgtt tcaaattagt cgttgctata
     2281 tatattgtct tatgtgtgga aaacgaataa gaaaaattag agaaagatga gagagcaggg
     2341 gcagaatgaa aatttgcatg gaaattgaag aagatcactg ctttttccga tgaatgatga
     2401 ctaaatagaa tgagttgaga ggaaaataat cataattcta attttaatta gaaatatgat
     2461 atggaaagta agcaaaacca caaggctttt gctctgaact tatttccgaa aaaaaaaagt
     2521 gaaactgaaa gttagaattt tcaaaataat attgcgagag tcaaccttca gaaaaattgg
     2581 cgagaaatca aatttgaatt ctctgaaaat ttagttaaaa tacatatgtt atccttcttt
     2641 gttttttggc cttagcctgc cttcaaggcg acctgagcct atgcgggtgc ctaataccga
     2701 aacacacata attttttttt ttaatcttta aatactgtaa cgagtcgcga gatccagcga
     2761 gacccataga atatgttgag cgctgcaaac aatcgtataa ctcttgttca aatttttcca
     2821 ctctgtattt ttgcaagcag ttcaacaaat ggtcatcatt tttataaccc atatctatat
     2881 tcgaatctga cataacaaat aatactaagc acatcctccc acatctcttt tcattcaagt
     2941 tgcgcaattc ccctaataac tatggatgga ggggggaccg agcccaaaga ttattcaaaa
     3001 cgataacgac tttttcatgc ggccaacgaa aaataaaact tttctcatat tctcctattt
     3061 cccatttcaa gaattacatc cccatgggac gttgaaatca catcatatta gtatatgtta
     3121 atagttcttt tcactggaat atcgccacgc cccccttccg gtggctcatt ggtttcgaga
     3181 agaaggcgga gccgaagtgg gttcttcttt ctttttttgt ggtagaagtc tagcaggatt
     3241 aagctactat tccattagag gatcttcttc ttccaaaaag tgaaaatccc gggattggcc
     3301 aaaggaccca aaggtatgtt tcgaatgata ctaacataac atagaacatt ttcaggagga
     3361 cccttggcta gcgtcgacgg atccggatga gtatgctatt gtattatttc gcgagtgctg
     3421 tgaagtcaat acagtttcac gtcgatgatg acattattga caagcttaac tactattaca
     3481 ccacagccat tatcacagtg ttcgcgattc tcgtctctgc taaacagtat gtcggttttc
     3541 caatccaatg ttgggtacct gcgacgttca ccgagcccat ggagcagtac acggaaaact
     3601 attgttgggt tcaaaacacc tactttctcc ctttacacga ctacattcca cataactatg
     3661 cagaacgtga aaatcgacaa atcgggtatt atcaatgggt tccgtttgtg ttagctcttg
     3721 aagctcttct tttctatgtg ccgactattg tatggagact gctcagctgg caatcaggaa
     3781 ttcatgtgca gtctctggtt caaatggctt gtgactccag attgttggat ttggaatcga
     3841 ggaacagagc gttgcaaacc atcgcaacaa atgtagaaga agcgctacac gtgaaacatc
     3901 aagtgatgtc cggaaatcgt ctgaaactgc tcaacttgat tatttgcaca cgatcttccg
     3961 gtgctgctgt cacgttcctc tatatctctg tcaaaattct gtacaccgtg aacatcgtcg
     4021 gccaaatctt tctactgaac acgtttctcg gtaaccgtag caaatggtac ggactgcaag
     4081 ttctcaacga cttgatgaac ggacgagaat gggaggagtc tggtcatttt ccacgtgtaa
     4141 cgttatgtga ctttgaagta aaagtactgg gaaacgtgca ccgacataca gtacaatgtg
     4201 tacttatgat taacatgttc aacgagaaga tctttctgtt cttgtggttc tggtacttcc
     4261 ttcttgccgg tgctactttg tgctcgcttt tctactggat ctacatttca gtggtgccaa
     4321 gcaggcagct caactttgtt ggaaagtatc tgactggaat cgagggatat aaaatggtgg
     4381 attcgcaatc actacgacgt tttgtatttc actttttaag acaggatggt gttttcctac
     4441 ttcgtatggt tgcaactcac gccggagaac taccctgtta cgagcttgcg aaaacgttat
     4501 ggaacaacta ctgtgacaat aaagaaggaa aaatgcacga cgtgtgagga tccccgagat
     4561 cttcagcgct cagcattcgt agaattgtaa gtttaaactt tctcatacta actaacgcgc
     4621 tctatttaaa ttttcagaat tcgctgtctc atcctacttt cacctagtta actgcttgtc
     4681 ttaaaatcta tgcttctctt tagtatctaa aattttccta gaagcttaca agtatataaa
     4741 tggtctcttc tcaataaagg ttgtatattt attcatctta ttgaatctgc catttcctcg
     4801 tttttgcgag tttatatacc ttccaatttt ctttctattg tattttcaac ttctaatttt
     4861 aattcaggga aactgcttca acgcatcggt accctatggt ctcaaagggt gaagaagata
     4921 acatggcaat tattaaagag tttatgcgtt tcaaggtgca tatggaggga tctgtcaatg
     4981 ggcatgagtt tgaaattgaa ggtgaaggag aaggccgacc atatgaggga acacaaaccg
     5041 caaaactaaa ggtaagttta aacatatata tactaactaa ccctgattat ttaaattttc
     5101 aggtaactaa aggcggacca ttaccattcg cctgggacat cctctctcca cagttcatgt
     5161 atggaagtaa agcttatgtt aaacatccgg cagatatacc agattatttg aaactttcat
     5221 tcccggaggg ttttaagtgg gaacgcgtaa tgaattttga agacggagga gttgttacag
     5281 tgacgcaaga ctcaaggtaa gtttaaacag ttcggtacta actaaccata catatttaaa
     5341 ttttcagcct ccaagatgga gaatttattt ataaagtcaa acttcgagga acgaatttcc
     5401 cctcggatgg atctgttatg cagaagaaga ctatgggatg ggaagcttca agtgaaagaa
     5461 tgtaccctga agacggtgct cttaagggag agattaaaca acgtcttaaa ttgaaagatg
     5521 gaggacatta cgatgctgag gtaagtttaa acatgatttt actaactaac taatctgatt
     5581 taaattttca ggtgaagaca acttacaaag ccaaaaaacc agttcagctg ccaggagcgt
     5641 acaatgttaa tattaaactg gatatcacct cccacaacga ggattacact atcgttgagc
     5701 aatatgaaag agctgaaggg cggcactcga caggtggcat ggatgaattg tataagtagg
     5761 agctcccgag atcttcagcg ctcagcattc gtagaattgt aagtttaaac tttctcatac
     5821 taactaacgc gctctattta aattttcaga attccaactg agcgccggtc gctaccatta
     5881 ccaacttgtc tggtgtcaaa aataataggg gccgctgtca tcagagtaag tttaaactga
     5941 gttctactaa ctaacgagta atatttaaat tttcagcatc tcgcgcccgt gcctctgact
     6001 tctaagtcca attactcttc aacatcccta catgctcttt ctccctgtgc tcccaccccc
     6061 tatttttgtt attatcaaaa aacttctctt aatttctttg ttttttagct tcttttaagt
     6121 cacctctaac aatgaaattg tgtagattca aaaatagaat taattcgtaa taaaaagtcg
     6181 aaaaaaattg tgctccctcc ccccattaat aataattcta tcccaaaatc tacacaatgt
     6241 tctgtgtaca cttcttatgt tttttacttc tgataaattt ttttgaaaca tcatagaaaa
     6301 aaccgcacac aaaatacctt atcatatgtt acgtttcagt ttatgaccgc aatttttatt
     6361 tcttcgcacg tctgggcctc tcatgacgtc aaatcatgct catcgtgaaa aagttttgga
     6421 gtatttttgg aatttttcaa tcaagtgaaa gtttatgaaa ttaattttcc tgcttttgct
     6481 ttttggggtt tcccctattg tttgtcaaga tttcgaggac ggcgtttttc ttgctaaaat
     6541 cacaagtatt gatgagcacg atgcaagaaa gatcggaaga aggtttgggt ttgaggctca
     6601 gtggaaggtg agtagaagtt gataatttga aagtggagta gtgtctatgg ggtttttgcc
     6661 ttaaatgaca gaatacattc ccaatatacc aaacataact gtttcctact agtcggccgt
     6721 acgggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     6781 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     6841 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     6901 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     6961 accgcatcag gcggccttaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     7021 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     7081 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     7141 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     7201 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     7261 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     7321 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     7381 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     7441 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     7501 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     7561 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     7621 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     7681 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     7741 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     7801 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     7861 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     7921 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     7981 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     8041 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     8101 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     8161 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     8221 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     8281 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     8341 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     8401 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     8461 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     8521 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     8581 tacctacagc gtgag
//
