LOCUS       sequence-355535-       10380 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pacr-5::UNC-9-UrSL-wCherry unc-54 3' UTR C.elegans neural expression
            of UNC-9-wCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10380)
  AUTHORS   Kawano T, Po MD, Gao S, Leung G, Ryu WS, Zhen M
  TITLE     An imbalancing act: gap junctions reduce the backward motor circuit 
            activity to bias C. elegans for forward locomotion.
  JOURNAL   Neuron. 2011 Nov 17;72(4):572-86. doi: 10.1016/j.neuron.2011.09.005.
  PUBMED    22099460
REFERENCE   2  (bases 1 to 10380)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2011.09"; journalName: "Neuron"; date: 
            "2011-11-17- 17"; volume: "72"; issue: "4"; pages: "572-86"
COMMENT     Sequence Label: pJH2140
FEATURES             Location/Qualifiers
     source          1..10380
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     133..152
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     386..403
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    520..541
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(556..561,562..579,580..586)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    594..610
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     599..621
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     618..634
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     618..634
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8583..8605)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8705..8724
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8802..8820)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8888..8992
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8993..9061,9062..9853)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9211..9230)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(10024..10380,1..232)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
       61 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
      121 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
      181 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
      241 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
      301 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
      361 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
      421 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
      481 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
      541 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
      601 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc tgtaagttta
      661 aacatgatct tactaactaa ctattctcat ttaaattttc agagcttaaa aatggctgaa
      721 atcactcaca acgatggata cgctaacaac ttggaaatga aataagcttg ggctgcagca
      781 tgcggccgca tttgttgaaa aaacgtacgg tcttcattta agagtgacat agtttcttct
      841 tacattttta atattaatct gatgtttcag atctgctcta ataatggctg gttcagcaca
      901 tcaaaacgag tacgactttt cgcaacgacg gcattcactt atcaacatgt tcaaggcgtt
      961 taaattgtaa acaatcttgt gatgcagcat tgctccaatg ataatttggg tatttcatag
     1021 ggtgttattt tgcatctgtt tatctatgaa gaagctgaaa actggaatta tcattagagc
     1081 aagttcgcaa gcagaccagc acacaaaata atgcaacaga atttggttcc caaagagctc
     1141 aataaagttt acttttcaca aatgcttcaa tttccgtttc ttccatttaa attgttctga
     1201 tgttatagct tctcgcactt tccctccatt tcaatttccc gcctattttc taccctcaaa
     1261 agttcctctc atagttttct tattgatgag gaacttttgg gcattagcaa aattgtgccc
     1321 tttgaattaa gttaaaatga attaaataat cttttattaa agcgaattcg catcatattt
     1381 taaatgcgcg gtattaataa aaataggttc gtcgatcaat aggtgttttc atattatcca
     1441 aatacacaat tgggcaattt gtaaaacttg aagaatgcgg caacgagttc tgtgcaagtc
     1501 tttaccttta atgcttccgt catcgttagc aaaaactgtg tcatgaaaga caagtgtata
     1561 agattattca aatacaattt tcttatggag tttttctagc ttttaaggat ttccaaatag
     1621 aatgatcatt ccttgaataa taattctcta ttttatcaat attttgtttt tttttctatt
     1681 ttagaaaggg cggagagtag tgtgtagtgg cggtttcacg ctctgatgat ctcatgccat
     1741 tgttcacctt ttccaaattg ttttaggtta ttcggagtac ttcttttctt tcttttccct
     1801 cttcattttc accctctgtt tgagcatgag tgctctctat tctttggtga tggaataaaa
     1861 agaggtgcca tcaaattcag aattttgtaa aatgttgaac tttagagttt ttagtattcg
     1921 gttagtgtga tgtttgtttt attttcaacc ctaacaaatc tcagttttct ggaaaagttg
     1981 tgcatttaca atgaatatat tgttttctgc atttcgtctg ttcttagatt attcagtgac
     2041 tttggaaatt taagctaatt cttgttgagc catttgacat aatttgtata gaaaacaaaa
     2101 atgtatactt tacaccgcga tttagatacg gcactgtgcg gaaactagca aacaattcag
     2161 agccgacttc cataaggttg ccaaaaaaat tttagtaatt tattaattca ataattaaat
     2221 gtatttatca cgaaaataaa atgaaaacat ttgttgttca aaattatata ttttcagaaa
     2281 gaaaaaacaa gtttttaaaa tttaattact acttctcatt atgagtctct caaaaagctc
     2341 aacatttcag ttagaataaa aattaaaaaa tatcaatgaa agtgtcccgt ctaattccat
     2401 ttttcactcc ccacactgtt caacgtcaaa acacattgtc attcagtcat tttggaaaaa
     2461 gcaccccgag cgcagaagca gtaggaaaaa cgagtctcga tgggggtagg ttgaacgagc
     2521 aaaaaaaacc aattcggcta attgaactcg actttttctc ccaattttac tctcaccaat
     2581 cgtgatgcgt atctaaatgg atacgcaaag aaaggaaaag aaagagcttt ttattttcag
     2641 atccacccac ggacacgttt ttggtagaac tattacatca ctttttctta gttttttctg
     2701 tgagcttgat gagaactgtg gtgtattttt tgtagataaa acaaaattta aacctacttc
     2761 ataagttcac acacctacct tatgtcagcc tagaaagtta ccacgatgat ggcagaaagt
     2821 tgcctctttg cctctaaatg ccgagtagtt tcaaaaaatg ttgacatttg atttggaatt
     2881 taagagtcct ctctgaaaat gtgtaattct gaaattttag tgcccatttc aaaaaaataa
     2941 catctgctca gcaaattgta tttcatcctc tctttatttt ttgttacccc gtcttacttc
     3001 ctctcttttt tatttattgt tttttcttgt ggtcctaata ataaaaatga aaagagaagc
     3061 tgttgtgctc ttccacaaca atgtgctctt ttcaatattc taaaatggca atggaattgg
     3121 caattgttgg agggttaaaa ttgccagaca aaagtcatct tctctatgtt tgttcttcat
     3181 cttcttttac ctctattcct tatccttctc aataacatcc tgaaactgac gatttaatga
     3241 gtatacttcc gtttatgaat gtatcatgat aatttcatgt ctgaaaaaga acggagaaat
     3301 gagaatgcat attctcaact tgaacgtata gccaagactt atgcctattt tcaatctgtt
     3361 tagaccctga caaacatttg accacacagg agtgtttggg acaacaacca tatttttgaa
     3421 aacatttttg aatgagaaat gctatcttct gtgggaagtt ttaaagcaag ctgatttctt
     3481 tatatagccc aaaataatta ttttttaata gattatgcaa attaaaatat ggagcttgat
     3541 ttgctgaagt gtctatttga tttgatttga tttgatttga tttattacga ttggaatcgt
     3601 gatggaataa aagcagtata gtataaagac agaaaaaata aatacacaat acataggaag
     3661 aataatcaaa aagttacgaa taaagtacaa acaagaaaag caattatcac ctgcaaaagt
     3721 tgattaattg gaacattaaa gtgaacatga aataggctat ttgggggtgt gaggagagtg
     3781 ggtgaatgat gggtaataag aggtgccaat ataagattcg tcatgctaat ttttcataga
     3841 actttagacc ctatttgtga aaaagtgtct tcttagtttg gttttgcatt tcccccagat
     3901 gaacttggtt ttggttctgg ttttgttgtt tttatcgatt ttgaaaaatt ggtccgtgtc
     3961 aatgtcaact ttatttaact ttaagcttta aagaataaac ttatcaaaat aaatcaaacg
     4021 gtactttttt tttgaaattt ttattcaaaa ttcaatcttt tcaagagcaa actgtaattt
     4081 tttttgtcat tcctatcgca aataaacgta aaattcagtt aatttaaaat gtgagaaatg
     4141 aggtaacaac tactatagaa aaataatgaa atgttcaagc gatgagcaat taaaatttac
     4201 gctcaaaaat taaccatgat gagaaaaaaa ttctgctctt caagttatac aagacttatg
     4261 agtgttaact ttatacttca aaaaataaat tgtattgaaa cacctttttt agagatttta
     4321 gattattaga ttgagaactg tacaaaaaaa cgagtgttcc aagatacatg agaatgatat
     4381 tttaacccga aacaatgaaa cattcaatca tacatattct catccttttc aactcgtcaa
     4441 cattccacgc cttgtctcac ttttttctat ttcccgtcca ctgggatttt gatttcccgc
     4501 ccgtttcgtc tcggcatttt ttttttgaaa gaggttactc tctcatcttt gacaccatct
     4561 tccataaaac acgagaaatg aataatggaa tcgagggagg ttcttgtttg ttttttttgt
     4621 gtgtttttgt tttgcatttt actcatattt tagaacttcg aaatctgatc atgactcgtt
     4681 ttgcgagaac cattgcttta aaatattttt attttcttga ggataactgt ttttttgaaa
     4741 aaaaaaacat ggaatctttg gaagtatgct gtaaattgag gaagtcttaa aatgtttcaa
     4801 cttccatatg aaaaattaaa tataatacag ttttgtttcc gttacaaaat ccaagacgtc
     4861 ttctcacaaa aaatcgccta aattttcatt taaaaatgtg tctttgcaaa agttaccagt
     4921 cctgtcaaat actcgcaaca aatatccaag aataactgac tgatctgatt tttgcagtta
     4981 gtttttcctc ttcaaactca aaatctgttt ttatttgaac cattcaatag tttaaacccc
     5041 atttgaatac gttttctcat ctttcctcgt caccagtttc aatgctttaa aaacaatttt
     5101 cagcatcccg ggattggcca aaggacccaa aggtatgttt cgaatgatac taacataaca
     5161 tagaacattt tcaggaggac ccttggctag cgtcgacgga tccggatgag tatgctattg
     5221 tattatttcg cgagtgctgt gaagtcaata cagtttcacg tcgatgatga cattattgac
     5281 aagcttaact actattacac cacagccatt atcacagtgt tcgcgattct cgtctctgct
     5341 aaacagtatg tcggttttcc aatccaatgt tgggtacctg cgacgttcac cgagcccatg
     5401 gagcagtaca cggaaaacta ttgttgggtt caaaacacct actttctccc tttacacgac
     5461 tacattccac ataactatgc agaacgtgaa aatcgacaaa tcgggtatta tcaatgggtt
     5521 ccgtttgtgt tagctcttga agctcttctt ttctatgtgc cgactattgt atggagactg
     5581 ctcagctggc aatcaggaat tcatgtgcag tctctggttc aaatggcttg tgactccaga
     5641 ttgttggatt tggaatcgag gaacagagcg ttgcaaacca tcgcaacaaa tgtagaagaa
     5701 gcgctacacg tgaaacatca agtgatgtcc ggaaatcgtc tgaaactgct caacttgatt
     5761 atttgcacac gatcttccgg tgctgctgtc acgttcctct atatctctgt caaaattctg
     5821 tacaccgtga acatcgtcgg ccaaatcttt ctactgaaca cgtttctcgg taaccgtagc
     5881 aaatggtacg gactgcaagt tctcaacgac ttgatgaacg gacgagaatg ggaggagtct
     5941 ggtcattttc cacgtgtaac gttatgtgac tttgaagtaa aagtactggg aaacgtgcac
     6001 cgacatacag tacaatgtgt acttatgatt aacatgttca acgagaagat ctttctgttc
     6061 ttgtggttct ggtacttcct tcttgccggt gctactttgt gctcgctttt ctactggatc
     6121 tacatttcag tggtgccaag caggcagctc aactttgttg gaaagtatct gactggaatc
     6181 gagggatata aaatggtgga ttcgcaatca ctacgacgtt ttgtatttca ctttttaaga
     6241 caggatggtg ttttcctact tcgtatggtt gcaactcacg ccggagaact accctgttac
     6301 gagcttgcga aaacgttatg gaacaactac tgtgacaata aagaaggaaa aatgcacgac
     6361 gtgtgaggat ccccgagatc ttcagcgctc agcattcgta gaattgtaag tttaaacttt
     6421 ctcatactaa ctaacgcgct ctatttaaat tttcagaatt cgctgtctca tcctactttc
     6481 acctagttaa ctgcttgtct taaaatctat gcttctcttt agtatctaaa attttcctag
     6541 aagcttacaa gtatataaat ggtctcttct caataaaggt tgtatattta ttcatcttat
     6601 tgaatctgcc atttcctcgt ttttgcgagt ttatatacct tccaattttc tttctattgt
     6661 attttcaact tctaatttta attcagggaa actgcttcaa cgcatcggta ccctatggtc
     6721 tcaaagggtg aagaagataa catggcaatt attaaagagt ttatgcgttt caaggtgcat
     6781 atggagggat ctgtcaatgg gcatgagttt gaaattgaag gtgaaggaga aggccgacca
     6841 tatgagggaa cacaaaccgc aaaactaaag gtaagtttaa acatatatat actaactaac
     6901 cctgattatt taaattttca ggtaactaaa ggcggaccat taccattcgc ctgggacatc
     6961 ctctctccac agttcatgta tggaagtaaa gcttatgtta aacatccggc agatatacca
     7021 gattatttga aactttcatt cccggagggt tttaagtggg aacgcgtaat gaattttgaa
     7081 gacggaggag ttgttacagt gacgcaagac tcaaggtaag tttaaacagt tcggtactaa
     7141 ctaaccatac atatttaaat tttcagcctc caagatggag aatttattta taaagtcaaa
     7201 cttcgaggaa cgaatttccc ctcggatgga tctgttatgc agaagaagac tatgggatgg
     7261 gaagcttcaa gtgaaagaat gtaccctgaa gacggtgctc ttaagggaga gattaaacaa
     7321 cgtcttaaat tgaaagatgg aggacattac gatgctgagg taagtttaaa catgatttta
     7381 ctaactaact aatctgattt aaattttcag gtgaagacaa cttacaaagc caaaaaacca
     7441 gttcagctgc caggagcgta caatgttaat attaaactgg atatcacctc ccacaacgag
     7501 gattacacta tcgttgagca atatgaaaga gctgaagggc ggcactcgac aggtggcatg
     7561 gatgaattgt ataagtagga gctcccgaga tcttcagcgc tcagcattcg tagaattgta
     7621 agtttaaact ttctcatact aactaacgcg ctctatttaa attttcagaa ttccaactga
     7681 gcgccggtcg ctaccattac caacttgtct ggtgtcaaaa ataatagggg ccgctgtcat
     7741 cagagtaagt ttaaactgag ttctactaac taacgagtaa tatttaaatt ttcagcatct
     7801 cgcgcccgtg cctctgactt ctaagtccaa ttactcttca acatccctac atgctctttc
     7861 tccctgtgct cccaccccct atttttgtta ttatcaaaaa acttctctta atttctttgt
     7921 tttttagctt cttttaagtc acctctaaca atgaaattgt gtagattcaa aaatagaatt
     7981 aattcgtaat aaaaagtcga aaaaaattgt gctccctccc cccattaata ataattctat
     8041 cccaaaatct acacaatgtt ctgtgtacac ttcttatgtt ttttacttct gataaatttt
     8101 tttgaaacat catagaaaaa accgcacaca aaatacctta tcatatgtta cgtttcagtt
     8161 tatgaccgca atttttattt cttcgcacgt ctgggcctct catgacgtca aatcatgctc
     8221 atcgtgaaaa agttttggag tatttttgga atttttcaat caagtgaaag tttatgaaat
     8281 taattttcct gcttttgctt tttggggttt cccctattgt ttgtcaagat ttcgaggacg
     8341 gcgtttttct tgctaaaatc acaagtattg atgagcacga tgcaagaaag atcggaagaa
     8401 ggtttgggtt tgaggctcag tggaaggtga gtagaagttg ataatttgaa agtggagtag
     8461 tgtctatggg gtttttgcct taaatgacag aatacattcc caatatacca aacataactg
     8521 tttcctacta gtcggccgta cgggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     8581 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     8641 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     8701 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     8761 agatgcgtaa ggagaaaata ccgcatcagg cggccttaag ggcctcgtga tacgcctatt
     8821 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     8881 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     8941 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     9001 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     9061 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     9121 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     9181 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     9241 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     9301 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     9361 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     9421 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     9481 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     9541 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     9601 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     9661 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     9721 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     9781 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     9841 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     9901 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     9961 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
    10021 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
    10081 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
    10141 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
    10201 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
    10261 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
    10321 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
//
