LOCUS       sequence-355536-        9410 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pnmr-1::TWK-18(gf)-UrSL-wCherry unc-54 3' UTR C.elegans neural 
            expression of TWK-18-wCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9410)
  AUTHORS   Kawano T, Po MD, Gao S, Leung G, Ryu WS, Zhen M
  TITLE     An imbalancing act: gap junctions reduce the backward motor circuit 
            activity to bias C. elegans for forward locomotion.
  JOURNAL   Neuron. 2011 Nov 17;72(4):572-86. doi: 10.1016/j.neuron.2011.09.005.
  PUBMED    22099460
REFERENCE   2  (bases 1 to 9410)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2011.09"; journalName: "Neuron"; date: 
            "2011-11-17- 17"; volume: "72"; issue: "4"; pages: "572-86"
COMMENT     Sequence Label: pJH2106
FEATURES             Location/Qualifiers
     source          1..9410
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..18
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    135..156
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(171..176,177..194,195..201)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    209..225
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     214..236
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     233..249
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     233..249
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7228..7250)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7350..7369
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7447..7465)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7533..7637
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7638..7706,7707..8498)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7856..7875)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8669..9257
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9158..9177
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
       61 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
      121 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
      181 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
      241 agctatgacc atgattacgc caagctgtaa gtttaaacat gatcttacta actaactatt
      301 ctcatttaaa ttttcagagc ttaaaaatgg ctgaaatcac tcacaacgat ggatacgcta
      361 acaacttgga aatgaaataa gcttgggctg cagcatgcgg ccgctgtggt ctcactttga
      421 acgacaaaag aaaaccaaat attgtaaagg aatagtacag ataaaaggga acaagtgata
      481 ccagctgatg gatgtaggtc agcgacaaaa gacaggagac gtatagtttg atgattatgg
      541 aaccaaactc agaatttaat gttggagacg ctataaaaat tgaattgaga aattgaaacc
      601 atattcttga atatctctca atttggaatt tggaagtagc cgtaaaatcg gctagctaga
      661 acataaaagg actgatttgc aaccttgacc attcatgata tcatttcaat aaaagaagag
      721 attccaatga ccaatcttca agaatcacaa taatcacaac aaaactaagc aaaagtaaca
      781 acaaattgtt caggtagaca agtagacatt tggacacatt ttgcggggat gtgatgactg
      841 tataataaaa ataagtggac actgagagag agaggaggtc agcaggaaaa aagaacatcg
      901 aactgcttga cgaattgaaa ttagaatgag gtgtcaggca ggtgacgatt acatggttaa
      961 ttattagtgt tctttttctt ctttttgtgg atgccgatta gttgaaataa ttgcatggaa
     1021 agtgagaatt caatgtgaat ctctttctta ttatgtttca agtggtatct ggatggaagc
     1081 ttgtactttt atgaatcgaa aattgcgccc aaggttgatt ttgagattat tcagtaaatt
     1141 tttgtgctga ttattcagtt aatgtagaac agagacgcgg atgaaaactt ttttaatttc
     1201 taaaaaaaaa gacaaaatgt ggttaaaaag tttggaaaat aattgttata tacttcttga
     1261 gagcttattc aaacggaaac caatcataaa tagtcgaata gtcatttaga aaagagaaac
     1321 tagttgatgt agagagattc aaaaagtaaa atatgcaaat gatccgtttc ttagatttag
     1381 taaacatcta ccaaatgaaa caggaattgt cgaaacaatt tagaaaagga gaaggaaaca
     1441 tttgtttatt ttccccttta aatggcggta gaggtaaaac agcaatacac atagtacaag
     1501 gttatactac tttcaaaaga atttgacatc ccgccgagaa aatgtggtga ttatatggta
     1561 aagttttcag ttggatatta tcttctacag acggtgattc aaaatgatca aaagggcaag
     1621 tgggtggtag gatttgaaaa attgagatat agaacgtgcg attttggtaa aactttttga
     1681 acacaaggca tatttttgaa ctttgaaaaa cgcaaaattt aaattttgtt aattacatat
     1741 attttgaaca gattatgaat ataagcaagg ctacataaaa tatgtatatt ttctttttac
     1801 aaatattcgt acactgttaa atttatagaa aaatcattta tattcagaaa aacctatttg
     1861 aacaattttt agttgctcaa taaccaattt gcacaaattt agaaaatata atgggggctt
     1921 ttaaaaattt aaatcctcag cgaatattcc tttgcgattt tttcgaacgt tttctttcag
     1981 attaaaaaat ctgaagcaga agtaaatgaa ctaccataga aaattcacca aggatcacat
     2041 ttttacaaag cctccgccca attgtaaact tgggaatttt tgcttgtttt cttttttttt
     2101 tcaaaaaagg aatagttgtt ttaaagtaat ttattcccct atgctttctc cagcaatcag
     2161 ttgttctccc catactttac acaattatca acaacttcac atagaaaaca aaacaacaaa
     2221 tcagaaattc ccaccggtca cagtcacaga ttccccatgt tttgttcatc tcatttctgc
     2281 aagaaagtga catcgctgcc acccaattcg acaaattgtc caagtggtca tcaggaagat
     2341 atgaccttct ttttaatgat aatttagact acaaagggcg tcaaaaaaga ttcaatgatc
     2401 aggggtaatt gctgtaaaat gtagtttgta tgtgtgttta ccgtagccta taactgtaaa
     2461 aattggtttt ttaaaaagta tttctgagat taaaaggctc cccaaggatg cacgattcta
     2521 gctagaaatg aacattccat gggttgtgaa gatgctttta tctcacttta gaatgtctga
     2581 ctccgcccct ctgtgtacga attgaagtgc tcttttgtga tgatcatagc tcctccttgc
     2641 ggcgacgccc gcacacagca cgagatatta cacggaatac ttatgtgatt cataggaaac
     2701 aaggaaatcg aaaaaataat aggattttga ggaggtattt ttagatgaat gcttatgatg
     2761 ttatgatgaa cccaagaaat tctgaataga catatgtcag aacggggtag gagctccagt
     2821 gtcgcttttg aaataaaaaa tcaagtggta tacttctcgt atataccctt ctgaactaat
     2881 aaaataattt atcttttgaa aaagtttgaa aaagaaaaat gcccaaaact tgaattttaa
     2941 tgttttgtat tttgagcata tcgttcaaac gtcgtgatct gaaagtagaa agatctaaat
     3001 attcgtgaca cagtttggtt tgaaggtctt agtggtaatt ttttattaca gaagcaacaa
     3061 aaagttgaca tattttcaga atttccaaaa aaacgtttaa aatttcaatt tcttcgatag
     3121 agttctaaaa attttttgga acggattttg taatatccgc tgataaccgt tcaagaacaa
     3181 tttgaatttt gaaataattt aatcaactag tatccagtgc tctttctcac tgctccaccc
     3241 atttttcaac ccatttcctt tactcaattt cgcccgtttt ccttttgctc aaatgtgccc
     3301 accaaatcat ctttcttcgt cttcttttct cttattcgga aggagaagag gtgaaagtag
     3361 aataagtttt tttgttgaaa agagaggagc acggccactt ttctcttatt tccatccttc
     3421 tactacctcg ttttactttc catttaaaaa cccgtttaaa gttccaaaat ttcaattatc
     3481 ttgcttctgt ttggaacacg acaaacttta gttttgttac agatacccgg gattggccaa
     3541 aggacccaaa ggtatgtttc gaatgatact aacataacat agaacatttt caggaggacc
     3601 cttggctagc gtcgacggat ccaaatggcg attgttgcgc aaggagtttc tacgattttg
     3661 acgacgtttc agaaaacgtt caaaggatta ctcccattga taattcttgt ggcttacact
     3721 ctattgggag catggatctt ctggatgatc gaaggagaaa acgaacgaga gatgctcatt
     3781 gagcaacaaa aagaaaggga tgaactcatc cggcgaactg tctataaaat taatcaattg
     3841 caaatcaaaa gacagagaag attaatgacg gccgaggaag aatacaacag aactgcaaaa
     3901 gtgttgacta cctttcaaga aactttggga attgtaccag ctgatatgga caaggatatt
     3961 cattggacat tcttgggatc aatcttctat tgcatgacag tctatacaac tattggttac
     4021 ggaaacattg ttcctggaac tggatggggt cgttttgcta ctatccttta cgcattcatt
     4081 ggaattccac tcacagtgct cagtctttac tgtcttggta gtctttttgc caagggatgc
     4141 aaaatgttgt ggagattttt tttgaagtcc accagggttg tgagcaaaga tttgtcaaat
     4201 aagatttctg aagctgccga taatattgaa gaaggaacaa cggcaattac tccatcagcc
     4261 gagaaaacag aaaataacga tgatgacttg ttgtctttcc ccatctccgg cctcttgctt
     4321 attaccgtca tctgggtcat tttctgtgcg gtcttgttca ccttcctaga agaatgggac
     4381 tttggaacat ctctctactt cacattgatt tctttcacaa ccatcggttt tggagacatt
     4441 cttccatcgg attacgattt cataccaatt gttggagttc ttcttctcat tggtctttct
     4501 ttggtctcta cagtgatgac actcattcaa cagcaaattg aagcattagc atcgggaatg
     4561 aaagataaca ttgaccaaga atatgctcgt gcattgaacg aggctcgtga agacggagag
     4621 gttgacgagc acgtagatcc agaagaagat ccggagaaca ataaaaagtc ctttgacgct
     4681 gttatctcca gaatgaactg gtccaagaga gggttgtatt atcttcttcc tgatagccag
     4741 aagaaagaac tcgcaaagca gtctgagaag aaaatgggaa gaaaaagtat taaaattcag
     4801 actgataacg atttgttgga aacactcatt cgggaggaaa ttttgaaggc ggagctcaac
     4861 aatgagatgc acaaatatac agcaccacga tcttcacacc agccaaaact tgtctattct
     4921 gatgttcgcg agaaggaggt tccaattgaa gttgtccgtg ttgagcattt caatcacgga
     4981 aatgaagact atctagagca tgacatctag tggatccccg agatcttcag cgctcagcat
     5041 tcgtagaatt gtaagtttaa actttctcat actaactaac gcgctctatt taaattttca
     5101 gaattcgctg tctcatccta ctttcaccta gttaactgct tgtcttaaaa tctatgcttc
     5161 tctttagtat ctaaaatttt cctagaagct tacaagtata taaatggtct cttctcaata
     5221 aaggttgtat atttattcat cttattgaat ctgccatttc ctcgtttttg cgagtttata
     5281 taccttccaa ttttctttct attgtatttt caacttctaa ttttaattca gggaaactgc
     5341 ttcaacgcat cggtacccta tggtctcaaa gggtgaagaa gataacatgg caattattaa
     5401 agagtttatg cgtttcaagg tgcatatgga gggatctgtc aatgggcatg agtttgaaat
     5461 tgaaggtgaa ggagaaggcc gaccatatga gggaacacaa accgcaaaac taaaggtaag
     5521 tttaaacata tatatactaa ctaaccctga ttatttaaat tttcaggtaa ctaaaggcgg
     5581 accattacca ttcgcctggg acatcctctc tccacagttc atgtatggaa gtaaagctta
     5641 tgttaaacat ccggcagata taccagatta tttgaaactt tcattcccgg agggttttaa
     5701 gtgggaacgc gtaatgaatt ttgaagacgg aggagttgtt acagtgacgc aagactcaag
     5761 gtaagtttaa acagttcggt actaactaac catacatatt taaattttca gcctccaaga
     5821 tggagaattt atttataaag tcaaacttcg aggaacgaat ttcccctcgg atggatctgt
     5881 tatgcagaag aagactatgg gatgggaagc ttcaagtgaa agaatgtacc ctgaagacgg
     5941 tgctcttaag ggagagatta aacaacgtct taaattgaaa gatggaggac attacgatgc
     6001 tgaggtaagt ttaaacatga ttttactaac taactaatct gatttaaatt ttcaggtgaa
     6061 gacaacttac aaagccaaaa aaccagttca gctgccagga gcgtacaatg ttaatattaa
     6121 actggatatc acctcccaca acgaggatta cactatcgtt gagcaatatg aaagagctga
     6181 agggcggcac tcgacaggtg gcatggatga attgtataag taggagctcc cgagatcttc
     6241 agcgctcagc attcgtagaa ttgtaagttt aaactttctc atactaacta acgcgctcta
     6301 tttaaatttt cagaattcca actgagcgcc ggtcgctacc attaccaact tgtctggtgt
     6361 caaaaataat aggggccgct gtcatcagag taagtttaaa ctgagttcta ctaactaacg
     6421 agtaatattt aaattttcag catctcgcgc ccgtgcctct gacttctaag tccaattact
     6481 cttcaacatc cctacatgct ctttctccct gtgctcccac cccctatttt tgttattatc
     6541 aaaaaacttc tcttaatttc tttgtttttt agcttctttt aagtcacctc taacaatgaa
     6601 attgtgtaga ttcaaaaata gaattaattc gtaataaaaa gtcgaaaaaa attgtgctcc
     6661 ctccccccat taataataat tctatcccaa aatctacaca atgttctgtg tacacttctt
     6721 atgtttttta cttctgataa atttttttga aacatcatag aaaaaaccgc acacaaaata
     6781 ccttatcata tgttacgttt cagtttatga ccgcaatttt tatttcttcg cacgtctggg
     6841 cctctcatga cgtcaaatca tgctcatcgt gaaaaagttt tggagtattt ttggaatttt
     6901 tcaatcaagt gaaagtttat gaaattaatt ttcctgcttt tgctttttgg ggtttcccct
     6961 attgtttgtc aagatttcga ggacggcgtt tttcttgcta aaatcacaag tattgatgag
     7021 cacgatgcaa gaaagatcgg aagaaggttt gggtttgagg ctcagtggaa ggtgagtaga
     7081 agttgataat ttgaaagtgg agtagtgtct atggggtttt tgccttaaat gacagaatac
     7141 attcccaata taccaaacat aactgtttcc tactagtcgg ccgtacgggc cctttcgtct
     7201 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     7261 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     7321 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     7381 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcggcc
     7441 ttaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     7501 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     7561 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     7621 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     7681 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     7741 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     7801 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     7861 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     7921 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     7981 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     8041 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     8101 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     8161 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     8221 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     8281 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     8341 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     8401 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     8461 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     8521 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     8581 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     8641 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     8701 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     8761 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     8821 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     8881 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     8941 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     9001 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     9061 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     9121 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     9181 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     9241 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     9301 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     9361 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
//
