LOCUS       sequence-355538-        9115 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Psra-11::TWK-18(gf)-UrSL-wCherry unc-54 3' UTR C.elegans neural 
            expression of TWK-18-wCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9115)
  AUTHORS   Kawano T, Po MD, Gao S, Leung G, Ryu WS, Zhen M
  TITLE     An imbalancing act: gap junctions reduce the backward motor circuit 
            activity to bias C. elegans for forward locomotion.
  JOURNAL   Neuron. 2011 Nov 17;72(4):572-86. doi: 10.1016/j.neuron.2011.09.005.
  PUBMED    22099460
REFERENCE   2  (bases 1 to 9115)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2011.09"; journalName: "Neuron"; date: 
            "2011-11-17- 17"; volume: "72"; issue: "4"; pages: "572-86"
COMMENT     Sequence Label: pJH2217
FEATURES             Location/Qualifiers
     source          1..9115
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     133..152
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     386..403
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    520..541
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(556..561,562..579,580..586)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    594..610
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     599..621
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     618..634
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     618..634
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7318..7340)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7440..7459
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7537..7555)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7623..7727
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7728..7796,7797..8588)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7946..7965)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8759..9115,1..232)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
       61 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
      121 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
      181 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
      241 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
      301 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
      361 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
      421 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
      481 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
      541 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
      601 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc tgtaagttta
      661 aacatgatct tactaactaa ctattctcat ttaaattttc agagcttaaa aatggctgaa
      721 atcactcaca acgatggata cgctaacaac ttggaaatga aataagcttg ggctgcagca
      781 tgcggccgca cgttttccct ttacattagc aaaaccgacc aacgaccgaa acctaagcat
      841 tatccaggcc taaacatatt ttaaaaaaag tgtagtttac caaatgggag acattttcct
      901 tgttctttgt gggatgcttt tgaaattgaa tagaaatctc tagattaatc aatgtgtttg
      961 ctccgatgcg cctagtctca aagccttctc atgttgcaaa caaatgcact aactaaacta
     1021 attaacaagt cacggaggag attcaaaaca tttcactttt caggaaagta gcatttttaa
     1081 atatattcat gttgtagatt tgtcattttg caatattttt tatcaaaatt ggattacacc
     1141 ctcttttgag cagtctgact tgttggctga cttgattttc ttttaaagtg atgaaatttt
     1201 attcatattt gatttttagt aaataaatta ttgcaaaccc cattatgcaa ctttgacagc
     1261 ttatatctca gttgccgttt gttttatgaa aaattgtcaa cttaccaaat aattcttagt
     1321 tacgatctga aatttttcga aaactctaat ctttagtcaa aaatgttcag caatgtaaac
     1381 attttttcat tacaaaaaaa gatgatgtag atgcatacat caagtatctc aaccggaaca
     1441 ccccctattt tgcaccatct ctgtcatctt tttcttcctt tttcagatgg gaaacataca
     1501 attaatgctc acatgtcatg tctagtcatt tgtttttttt tgtttgtttt taatccgaat
     1561 ctcttattca tctttagacg ttccattcgg cagaggcgag ttggggcgcc tcttactcgc
     1621 ctcaaatttt gtgtttgcac tcataatcaa agaataaatt atcttgccca ttttggtcct
     1681 tgaccatttg tgtaggcaat aggcaggcag acatattttc ttaaacagaa gaccgaactg
     1741 tgagtgtaca aaaataaatt aaatattacc tacctttgtt agaatttgga ataagattaa
     1801 aaaattcgtt gtcctctgtg gaattgctca ttattagatt tttacgaaat catgcggatt
     1861 agaaagttta atatttatgt acagtgcgtc caacttctat agcacccccc tccaattcgg
     1921 ccgtttggag cttttcaaaa tatttttcgg aaaagtgtta gtgcagttcg atagcaaata
     1981 ttgaaaaacc tatgctcccc aatttttatc atgatatctc aaaaatcacg gaaattgggt
     2041 caaaatatct gaaaaatggc cgtcgaactt ctatagcacc ccctctgatt tttgacaaat
     2101 ttctgtaaat tcggggtttt cttcgtagtt aatgaattta ttttgtaaat aacttcacac
     2161 caaaataatc aggtggtttc actttcacat ttaaaaaata aatataacac ttgaaaacac
     2221 gtggaatgcc tgggattgat caaaaattga tcaaaatgtt cttctccgtc tattcattac
     2281 tatgtaatta gttgtagaaa gcatttattc atatgaacca aatggtaaag ggagcaactt
     2341 cagtggtttt taaaaggctc gatttaacca tgaaaacgag cgttcggagg cacaaagctg
     2401 aaggttttga cttcgtattg tacagaactt cagctcatcc tgctcaaatg tctttgaagc
     2461 ttcgattttg attagatgtc tttgaaaatt tatacggaaa agatcatgag accgctcaaa
     2521 aagaggacaa aggcaaagcg aggccgcaag tctttttttc aagctaacat gcgaaaattt
     2581 tagacagaaa agtaagatat acctttccaa atgacgattt tgctagttgt atttataact
     2641 caagttaaat ttagcgaatt cgctcattga atcttttttt ggtcaagtaa tgcaaaagct
     2701 accagacaaa acattgaacc tgcgattgta gatgtctggc atctgattta cccatggatt
     2761 tccacggatt ttttgttcat ataaaataat ttttaatttt tgctataatg tagtaatttt
     2821 tagtcgaaaa tgtattccca accattatct ctgcgcctcc gaacgctcgt tttcatggtt
     2881 aaatcgaacc ttttaaaaac cactgaagtt gctcccttta ccatttggtt catatgaata
     2941 aatgctttct acaactaatt atatagtaat gaatagacgg agaagaacat tttgatctat
     3001 tttggtgctg cgaaaatcat tgggagccaa gatatggcct ctgaagcaaa taagaacgtt
     3061 gaggggggtg ctatagaagt tggacgcact gtatttccaa ttatttttta agaatttcga
     3121 ttttataaac agcctttgct ccgcctattt gagctcatga tgtttctctt ttgttttcaa
     3181 gattgttgat tccttcctat tgtcctattg tgatttttaa atttttaaat tgctcattaa
     3241 cttgattttt atttagtaca tatctctaat atgttgtttc acattgcacc aaccggtctc
     3301 aatttttgaa attatttatt gttatgtttg aatattttaa acaaatttaa aaatttagaa
     3361 aaattgctga gctgttgcct aaactcataa gtgtttcaaa atttgctacg attttgcatg
     3421 tactttttaa tattttatat gctttttcgt attccaaaac aaacaattta gtttacttta
     3481 caaattatga acaaaaaaaa tttgatgtaa cggagagaaa attattaaac gttacatgta
     3541 tttttcgatc gtatcgagaa aaatgcaaaa aatatcaaat tgtaattttt ccgcacatct
     3601 ccgcaacaca caattcccgg gattggccaa aggacccaaa ggtatgtttc gaatgatact
     3661 aacataacat agaacatttt caggaggacc cttggctagc gtcgacggat ccaaatggcg
     3721 attgttgcgc aaggagtttc tacgattttg acgacgtttc agaaaacgtt caaaggatta
     3781 ctcccattga taattcttgt ggcttacact ctattgggag catggatctt ctggatgatc
     3841 gaaggagaaa acgaacgaga gatgctcatt gagcaacaaa aagaaaggga tgaactcatc
     3901 cggcgaactg tctataaaat taatcaattg caaatcaaaa gacagagaag attaatgacg
     3961 gccgaggaag aatacaacag aactgcaaaa gtgttgacta cctttcaaga aactttggga
     4021 attgtaccag ctgatatgga caaggatatt cattggacat tcttgggatc aatcttctat
     4081 tgcatgacag tctatacaac tattggttac ggaaacattg ttcctggaac tggatggggt
     4141 cgttttgcta ctatccttta cgcattcatt ggaattccac tcacagtgct cagtctttac
     4201 tgtcttggta gtctttttgc caagggatgc aaaatgttgt ggagattttt tttgaagtcc
     4261 accagggttg tgagcaaaga tttgtcaaat aagatttctg aagctgccga taatattgaa
     4321 gaaggaacaa cggcaattac tccatcagcc gagaaaacag aaaataacga tgatgacttg
     4381 ttgtctttcc ccatctccgg cctcttgctt attaccgtca tctgggtcat tttctgtgcg
     4441 gtcttgttca ccttcctaga agaatgggac tttggaacat ctctctactt cacattgatt
     4501 tctttcacaa ccatcggttt tggagacatt cttccatcgg attacgattt cataccaatt
     4561 gttggagttc ttcttctcat tggtctttct ttggtctcta cagtgatgac actcattcaa
     4621 cagcaaattg aagcattagc atcgggaatg aaagataaca ttgaccaaga atatgctcgt
     4681 gcattgaacg aggctcgtga agacggagag gttgacgagc acgtagatcc agaagaagat
     4741 ccggagaaca ataaaaagtc ctttgacgct gttatctcca gaatgaactg gtccaagaga
     4801 gggttgtatt atcttcttcc tgatagccag aagaaagaac tcgcaaagca gtctgagaag
     4861 aaaatgggaa gaaaaagtat taaaattcag actgataacg atttgttgga aacactcatt
     4921 cgggaggaaa ttttgaaggc ggagctcaac aatgagatgc acaaatatac agcaccacga
     4981 tcttcacacc agccaaaact tgtctattct gatgttcgcg agaaggaggt tccaattgaa
     5041 gttgtccgtg ttgagcattt caatcacgga aatgaagact atctagagca tgacatctag
     5101 tggatccccg agatcttcag cgctcagcat tcgtagaatt gtaagtttaa actttctcat
     5161 actaactaac gcgctctatt taaattttca gaattcgctg tctcatccta ctttcaccta
     5221 gttaactgct tgtcttaaaa tctatgcttc tctttagtat ctaaaatttt cctagaagct
     5281 tacaagtata taaatggtct cttctcaata aaggttgtat atttattcat cttattgaat
     5341 ctgccatttc ctcgtttttg cgagtttata taccttccaa ttttctttct attgtatttt
     5401 caacttctaa ttttaattca gggaaactgc ttcaacgcat cggtacccta tggtctcaaa
     5461 gggtgaagaa gataacatgg caattattaa agagtttatg cgtttcaagg tgcatatgga
     5521 gggatctgtc aatgggcatg agtttgaaat tgaaggtgaa ggagaaggcc gaccatatga
     5581 gggaacacaa accgcaaaac taaaggtaag tttaaacata tatatactaa ctaaccctga
     5641 ttatttaaat tttcaggtaa ctaaaggcgg accattacca ttcgcctggg acatcctctc
     5701 tccacagttc atgtatggaa gtaaagctta tgttaaacat ccggcagata taccagatta
     5761 tttgaaactt tcattcccgg agggttttaa gtgggaacgc gtaatgaatt ttgaagacgg
     5821 aggagttgtt acagtgacgc aagactcaag gtaagtttaa acagttcggt actaactaac
     5881 catacatatt taaattttca gcctccaaga tggagaattt atttataaag tcaaacttcg
     5941 aggaacgaat ttcccctcgg atggatctgt tatgcagaag aagactatgg gatgggaagc
     6001 ttcaagtgaa agaatgtacc ctgaagacgg tgctcttaag ggagagatta aacaacgtct
     6061 taaattgaaa gatggaggac attacgatgc tgaggtaagt ttaaacatga ttttactaac
     6121 taactaatct gatttaaatt ttcaggtgaa gacaacttac aaagccaaaa aaccagttca
     6181 gctgccagga gcgtacaatg ttaatattaa actggatatc acctcccaca acgaggatta
     6241 cactatcgtt gagcaatatg aaagagctga agggcggcac tcgacaggtg gcatggatga
     6301 attgtataag taggagctcc cgagatcttc agcgctcagc attcgtagaa ttgtaagttt
     6361 aaactttctc atactaacta acgcgctcta tttaaatttt cagaattcca actgagcgcc
     6421 ggtcgctacc attaccaact tgtctggtgt caaaaataat aggggccgct gtcatcagag
     6481 taagtttaaa ctgagttcta ctaactaacg agtaatattt aaattttcag catctcgcgc
     6541 ccgtgcctct gacttctaag tccaattact cttcaacatc cctacatgct ctttctccct
     6601 gtgctcccac cccctatttt tgttattatc aaaaaacttc tcttaatttc tttgtttttt
     6661 agcttctttt aagtcacctc taacaatgaa attgtgtaga ttcaaaaata gaattaattc
     6721 gtaataaaaa gtcgaaaaaa attgtgctcc ctccccccat taataataat tctatcccaa
     6781 aatctacaca atgttctgtg tacacttctt atgtttttta cttctgataa atttttttga
     6841 aacatcatag aaaaaaccgc acacaaaata ccttatcata tgttacgttt cagtttatga
     6901 ccgcaatttt tatttcttcg cacgtctggg cctctcatga cgtcaaatca tgctcatcgt
     6961 gaaaaagttt tggagtattt ttggaatttt tcaatcaagt gaaagtttat gaaattaatt
     7021 ttcctgcttt tgctttttgg ggtttcccct attgtttgtc aagatttcga ggacggcgtt
     7081 tttcttgcta aaatcacaag tattgatgag cacgatgcaa gaaagatcgg aagaaggttt
     7141 gggtttgagg ctcagtggaa ggtgagtaga agttgataat ttgaaagtgg agtagtgtct
     7201 atggggtttt tgccttaaat gacagaatac attcccaata taccaaacat aactgtttcc
     7261 tactagtcgg ccgtacgggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     7321 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     7381 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     7441 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     7501 cgtaaggaga aaataccgca tcaggcggcc ttaagggcct cgtgatacgc ctatttttat
     7561 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     7621 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     7681 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     7741 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     7801 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     7861 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     7921 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     7981 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     8041 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     8101 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     8161 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     8221 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     8281 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     8341 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     8401 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     8461 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     8521 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     8581 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     8641 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     8701 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     8761 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     8821 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     8881 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     8941 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     9001 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     9061 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaac
//
