LOCUS       sequence-355543-        9771 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pacr-5::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans B-MN and 
            other neurals expression of miniSOG-SL2 RFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9771)
  AUTHORS   Gao S, Guan SA, Fouad AD, Meng J, Kawano T, Huang YC, Li Y, Alcaire 
            S, Hung W, Lu Y, Qi YB, Jin Y, Alkema M, Fang-Yen C, Zhen M
  TITLE     Excitatory motor neurons are local oscillators for backward 
            locomotion.
  JOURNAL   Elife. 2018 Jan 23;7. pii: 29915. doi: 10.7554/eLife.29915.
  PUBMED    29360035
REFERENCE   2  (bases 1 to 9771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-01-23"; volume: "7. pii: 29915. doi"; pages: " 
            10.7554/eLife.29915"
COMMENT     Sequence Label: pJH2842
FEATURES             Location/Qualifiers
     source          1..9771
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     98..117
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     351..368
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    485..506
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(521..526,527..544,545..551)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    559..575
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     564..586
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     583..599
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     583..599
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             5402..5722
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     primer_bind     complement(7939..7961)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8061..8080
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8158..8176)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8244..8348
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8349..8417,8418..9209)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8567..8586)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9380..9771,1..197)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
       61 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
      121 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
      181 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
      241 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
      301 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
      361 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
      421 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
      481 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
      541 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
      601 atgattacgc caagctgtaa gtttaaacat gatcttacta actaactatt ctcatttaaa
      661 ttttcagagc ttaaaaatgg ctgaaatcac tcacaacgat ggatacgcta acaacttgga
      721 aatgaaataa gcttgggctg cagcatgcgg ccgcatttgt tgaaaaaacg tacggtcttc
      781 atttaagagt gacatagttt cttcttacat ttttaatatt aatctgatgt ttcagatctg
      841 ctctaataat ggctggttca gcacatcaaa acgagtacga cttttcgcaa cgacggcatt
      901 cacttatcaa catgttcaag gcgtttaaat tgtaaacaat cttgtgatgc agcattgctc
      961 caatgataat ttgggtattt catagggtgt tattttgcat ctgtttatct atgaagaagc
     1021 tgaaaactgg aattatcatt agagcaagtt cgcaagcaga ccagcacaca aaataatgca
     1081 acagaatttg gttcccaaag agctcaataa agtttacttt tcacaaatgc ttcaatttcc
     1141 gtttcttcca tttaaattgt tctgatgtta tagcttctcg cactttccct ccatttcaat
     1201 ttcccgccta ttttctaccc tcaaaagttc ctctcatagt tttcttattg atgaggaact
     1261 tttgggcatt agcaaaattg tgccctttga attaagttaa aatgaattaa ataatctttt
     1321 attaaagcga attcgcatca tattttaaat gcgcggtatt aataaaaata ggttcgtcga
     1381 tcaataggtg ttttcatatt atccaaatac acaattgggc aatttgtaaa acttgaagaa
     1441 tgcggcaacg agttctgtgc aagtctttac ctttaatgct tccgtcatcg ttagcaaaaa
     1501 ctgtgtcatg aaagacaagt gtataagatt attcaaatac aattttctta tggagttttt
     1561 ctagctttta aggatttcca aatagaatga tcattccttg aataataatt ctctatttta
     1621 tcaatatttt gttttttttt ctattttaga aagggcggag agtagtgtgt agtggcggtt
     1681 tcacgctctg atgatctcat gccattgttc accttttcca aattgtttta ggttattcgg
     1741 agtacttctt ttctttcttt tccctcttca ttttcaccct ctgtttgagc atgagtgctc
     1801 tctattcttt ggtgatggaa taaaaagagg tgccatcaaa ttcagaattt tgtaaaatgt
     1861 tgaactttag agtttttagt attcggttag tgtgatgttt gttttatttt caaccctaac
     1921 aaatctcagt tttctggaaa agttgtgcat ttacaatgaa tatattgttt tctgcatttc
     1981 gtctgttctt agattattca gtgactttgg aaatttaagc taattcttgt tgagccattt
     2041 gacataattt gtatagaaaa caaaaatgta tactttacac cgcgatttag atacggcact
     2101 gtgcggaaac tagcaaacaa ttcagagccg acttccataa ggttgccaaa aaaattttag
     2161 taatttatta attcaataat taaatgtatt tatcacgaaa ataaaatgaa aacatttgtt
     2221 gttcaaaatt atatattttc agaaagaaaa aacaagtttt taaaatttaa ttactacttc
     2281 tcattatgag tctctcaaaa agctcaacat ttcagttaga ataaaaatta aaaaatatca
     2341 atgaaagtgt cccgtctaat tccatttttc actccccaca ctgttcaacg tcaaaacaca
     2401 ttgtcattca gtcattttgg aaaaagcacc ccgagcgcag aagcagtagg aaaaacgagt
     2461 ctcgatgggg gtaggttgaa cgagcaaaaa aaaccaattc ggctaattga actcgacttt
     2521 ttctcccaat tttactctca ccaatcgtga tgcgtatcta aatggatacg caaagaaagg
     2581 aaaagaaaga gctttttatt ttcagatcca cccacggaca cgtttttggt agaactatta
     2641 catcactttt tcttagtttt ttctgtgagc ttgatgagaa ctgtggtgta ttttttgtag
     2701 ataaaacaaa atttaaacct acttcataag ttcacacacc taccttatgt cagcctagaa
     2761 agttaccacg atgatggcag aaagttgcct ctttgcctct aaatgccgag tagtttcaaa
     2821 aaatgttgac atttgatttg gaatttaaga gtcctctctg aaaatgtgta attctgaaat
     2881 tttagtgccc atttcaaaaa aataacatct gctcagcaaa ttgtatttca tcctctcttt
     2941 attttttgtt accccgtctt acttcctctc ttttttattt attgtttttt cttgtggtcc
     3001 taataataaa aatgaaaaga gaagctgttg tgctcttcca caacaatgtg ctcttttcaa
     3061 tattctaaaa tggcaatgga attggcaatt gttggagggt taaaattgcc agacaaaagt
     3121 catcttctct atgtttgttc ttcatcttct tttacctcta ttccttatcc ttctcaataa
     3181 catcctgaaa ctgacgattt aatgagtata cttccgttta tgaatgtatc atgataattt
     3241 catgtctgaa aaagaacgga gaaatgagaa tgcatattct caacttgaac gtatagccaa
     3301 gacttatgcc tattttcaat ctgtttagac cctgacaaac atttgaccac acaggagtgt
     3361 ttgggacaac aaccatattt ttgaaaacat ttttgaatga gaaatgctat cttctgtggg
     3421 aagttttaaa gcaagctgat ttctttatat agcccaaaat aattattttt taatagatta
     3481 tgcaaattaa aatatggagc ttgatttgct gaagtgtcta tttgatttga tttgatttga
     3541 tttgatttat tacgattgga atcgtgatgg aataaaagca gtatagtata aagacagaaa
     3601 aaataaatac acaatacata ggaagaataa tcaaaaagtt acgaataaag tacaaacaag
     3661 aaaagcaatt atcacctgca aaagttgatt aattggaaca ttaaagtgaa catgaaatag
     3721 gctatttggg ggtgtgagga gagtgggtga atgatgggta ataagaggtg ccaatataag
     3781 attcgtcatg ctaatttttc atagaacttt agaccctatt tgtgaaaaag tgtcttctta
     3841 gtttggtttt gcatttcccc cagatgaact tggttttggt tctggttttg ttgtttttat
     3901 cgattttgaa aaattggtcc gtgtcaatgt caactttatt taactttaag ctttaaagaa
     3961 taaacttatc aaaataaatc aaacggtact ttttttttga aatttttatt caaaattcaa
     4021 tcttttcaag agcaaactgt aatttttttt gtcattccta tcgcaaataa acgtaaaatt
     4081 cagttaattt aaaatgtgag aaatgaggta acaactacta tagaaaaata atgaaatgtt
     4141 caagcgatga gcaattaaaa tttacgctca aaaattaacc atgatgagaa aaaaattctg
     4201 ctcttcaagt tatacaagac ttatgagtgt taactttata cttcaaaaaa taaattgtat
     4261 tgaaacacct tttttagaga ttttagatta ttagattgag aactgtacaa aaaaacgagt
     4321 gttccaagat acatgagaat gatattttaa cccgaaacaa tgaaacattc aatcatacat
     4381 attctcatcc ttttcaactc gtcaacattc cacgccttgt ctcacttttt tctatttccc
     4441 gtccactggg attttgattt cccgcccgtt tcgtctcggc attttttttt tgaaagaggt
     4501 tactctctca tctttgacac catcttccat aaaacacgag aaatgaataa tggaatcgag
     4561 ggaggttctt gtttgttttt tttgtgtgtt tttgttttgc attttactca tattttagaa
     4621 cttcgaaatc tgatcatgac tcgttttgcg agaaccattg ctttaaaata tttttatttt
     4681 cttgaggata actgtttttt tgaaaaaaaa aacatggaat ctttggaagt atgctgtaaa
     4741 ttgaggaagt cttaaaatgt ttcaacttcc atatgaaaaa ttaaatataa tacagttttg
     4801 tttccgttac aaaatccaag acgtcttctc acaaaaaatc gcctaaattt tcatttaaaa
     4861 atgtgtcttt gcaaaagtta ccagtcctgt caaatactcg caacaaatat ccaagaataa
     4921 ctgactgatc tgatttttgc agttagtttt tcctcttcaa actcaaaatc tgtttttatt
     4981 tgaaccattc aatagtttaa accccatttg aatacgtttt ctcatctttc ctcgtcacca
     5041 gtttcaatgc tttaaaaaca attttcagca tcccgggatt ggccaaagga cccaaaggta
     5101 tgtttcgaat gatactaaca taacatagaa cattttcagg aggacccttg gctagcgtcg
     5161 acggatcccg atgtcggaca caattcttgg tttcaacaaa tcaaacgtcg ttttggctgc
     5221 tggaattgct ggagccgctt tcctcggcta ctgcatttac ttcgatcata agagaatcaa
     5281 cgctccagac tacaaggaca agattaggca aagtcagtgt tttaacaaca tatttccttc
     5341 ggatttttat ctaaaaacaa cttattttct ttcagagaga cgtgcccagg ctggggatcc
     5401 tatggagaaa agtttcgtga taactgatcc acggctgcca gacaatccca tcatcttcgc
     5461 atccgatggc ttcctggagc tgaccgagta ttccagagag gagatcctgg gccgcaatgg
     5521 ccgctttctg cagggaccag agacagacca ggccacagtg cagaagattc gcgatgccat
     5581 tagagatcag cgcgagatta ccgtgcagct gataaactac acaaaaagcg ggaagaaatt
     5641 ctggaacctc ctgcacctcc agcccatgag ggaccagaag ggtgagctcc agtatttcat
     5701 cggagtgcag ctggatggat aaagatcccc gagatcttca gcgctcagca ttcgtagaat
     5761 tgtaagttta aactttctca tactaactaa cgcgctctat ttaaattttc agaattcgct
     5821 gtctcatcct actttcacct agttaactgc ttgtcttaaa atctatgctt ctctttagta
     5881 tctaaaattt tcctagaagc ttacaagtat ataaatggtc tcttctcaat aaaggttgta
     5941 tatttattca tcttattgaa tctgccattt cctcgttttt gcgagtttat ataccttcca
     6001 attttctttc tattgtattt tcaacttcta attttaattc agggaaactg cttcaacgca
     6061 tcggtaccct atggtctcaa agggtgaaga agataacatg gcaattatta aagagtttat
     6121 gcgtttcaag gtgcatatgg agggatctgt caatgggcat gagtttgaaa ttgaaggtga
     6181 aggagaaggc cgaccatatg agggaacaca aaccgcaaaa ctaaaggtaa gtttaaacat
     6241 atatatacta actaaccctg attatttaaa ttttcaggta actaaaggcg gaccattacc
     6301 attcgcctgg gacatcctct ctccacagtt catgtatgga agtaaagctt atgttaaaca
     6361 tccggcagat ataccagatt atttgaaact ttcattcccg gagggtttta agtgggaacg
     6421 cgtaatgaat tttgaagacg gaggagttgt tacagtgacg caagactcaa ggtaagttta
     6481 aacagttcgg tactaactaa ccatacatat ttaaattttc agcctccaag atggagaatt
     6541 tatttataaa gtcaaacttc gaggaacgaa tttcccctcg gatggatctg ttatgcagaa
     6601 gaagactatg ggatgggaag cttcaagtga aagaatgtac cctgaagacg gtgctcttaa
     6661 gggagagatt aaacaacgtc ttaaattgaa agatggagga cattacgatg ctgaggtaag
     6721 tttaaacatg attttactaa ctaactaatc tgatttaaat tttcaggtga agacaactta
     6781 caaagccaaa aaaccagttc agctgccagg agcgtacaat gttaatatta aactggatat
     6841 cacctcccac aacgaggatt acactatcgt tgagcaatat gaaagagctg aagggcggca
     6901 ctcgacaggt ggcatggatg aattgtataa gtaggagctc ccgagatctt cagcgctcag
     6961 cattcgtaga attgtaagtt taaactttct catactaact aacgcgctct atttaaattt
     7021 tcagaattcc aactgagcgc cggtcgctac cattaccaac ttgtctggtg tcaaaaataa
     7081 taggggccgc tgtcatcaga gtaagtttaa actgagttct actaactaac gagtaatatt
     7141 taaattttca gcatctcgcg cccgtgcctc tgacttctaa gtccaattac tcttcaacat
     7201 ccctacatgc tctttctccc tgtgctccca ccccctattt ttgttattat caaaaaactt
     7261 ctcttaattt ctttgttttt tagcttcttt taagtcacct ctaacaatga aattgtgtag
     7321 attcaaaaat agaattaatt cgtaataaaa agtcgaaaaa aattgtgctc cctcccccca
     7381 ttaataataa ttctatccca aaatctacac aatgttctgt gtacacttct tatgtttttt
     7441 acttctgata aatttttttg aaacatcata gaaaaaaccg cacacaaaat accttatcat
     7501 atgttacgtt tcagtttatg accgcaattt ttatttcttc gcacgtctgg gcctctcatg
     7561 acgtcaaatc atgctcatcg tgaaaaagtt ttggagtatt tttggaattt ttcaatcaag
     7621 tgaaagttta tgaaattaat tttcctgctt ttgctttttg gggtttcccc tattgtttgt
     7681 caagatttcg aggacggcgt ttttcttgct aaaatcacaa gtattgatga gcacgatgca
     7741 agaaagatcg gaagaaggtt tgggtttgag gctcagtgga aggtgagtag aagttgataa
     7801 tttgaaagtg gagtagtgtc tatggggttt ttgccttaaa tgacagaata cattcccaat
     7861 ataccaaaca taactgtttc ctactagtcg gccgtacggg ccctttcgtc tcgcgcgttt
     7921 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     7981 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     8041 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     8101 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcggc cttaagggcc
     8161 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     8221 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     8281 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     8341 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     8401 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     8461 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     8521 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     8581 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     8641 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     8701 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     8761 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     8821 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     8881 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     8941 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     9001 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     9061 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     9121 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     9181 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     9241 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     9301 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     9361 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     9421 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     9481 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     9541 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     9601 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     9661 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     9721 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag c
//
