LOCUS       sequence-355544-        2604 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-GC2
FEATURES             Location/Qualifiers
     source          1..2604
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     342..361
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(568..573,574..590,591..596)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             644..1834
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(996..1015)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2008..2027)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2009..2027)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2032..2048)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2032..2048)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      join(2457..2604,1..441)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
       61 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
      121 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
      181 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
      241 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
      301 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
      361 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
      421 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
      481 tgctggcctt ttgctcacat gttctttcct gcgttatcca cagaatcagg ggataacgca
      541 ggaaagaaca tgaattaatt ctcatgtttg acagcttatc atcgattagc tttaatgcgg
      601 tagtttatca cagttaaatt gctaacgcag tcaggcaccg tgtatgaaat ctaacaatgc
      661 gctcatcgtc atcctcggca ccgtcaccct ggatgctgta ggcataggct tggttatgcc
      721 ggtactgccg ggcctcttgc gggatatcgt ccattccgac agcatcgcca gtcactatgg
      781 cgtgctgcta gcgctatatg cgttgatgca atttctatgc gcacccgttc tcggagcact
      841 gtccgaccgc tttggccgcc gcccagtcct gctcgcttcg ctacttggag ccactatcga
      901 ctacgcgatc atggcgacca cacccgtcct gtggattctc tacgccggac gcatcgtggc
      961 cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg
     1021 ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc
     1081 aggccccgtg gccgggggac tgttgggcgc catctcctta catgcaccat tccttgcggc
     1141 ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa
     1201 gggagagcgc cgacccatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc
     1261 gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg
     1321 acaggtgccg gcagcgctct gggtcatttt cggcgaggac cgctttcgct ggagcgcgac
     1381 gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc aagccttcgt
     1441 cactggtccc gccaccaaac gtttcggcga gaagcaggcc attatcgccg gcatggcggc
     1501 cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat
     1561 tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag
     1621 gcaggtagat gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcct
     1681 aacttcgatc attggaccgc tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg
     1741 gaacgggttg gcatggattg taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg
     1801 tcgcggtgca tggagccggg ccacctcgac ctgaatggaa gccggcggca cctcgctaac
     1861 ggattcacca ctccaagaat tggagccaat caattcttgc ggagaactgt gaatgcgcaa
     1921 accaaccctt gatcggggaa gaacagtatg tcgagctatt ttttgactta ctggggatca
     1981 agcctgattg ggagaaaata aaatatcccc tatagtgagt cgtattacat ggtcatagct
     2041 gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa
     2101 aatatatcat catgcctcct ctagaccaaa gctggtctca accacttgcg ctggggttag
     2161 tccgtgggct tgacagagaa ctggtagaag gcgttggacg gtttcaagag cctgtttgcc
     2221 accgtcatga ctagcaatcg ctacaacttg ctccggagtc agaccgtggg cttgacagag
     2281 aactggcagt aggcgctgta cggtttcaag agcctgtttg ccaccattgt tactagcaat
     2341 cgctacaacc tgcgagacca tatctggtcg agccacccat gaccaaaatc ccttaacgtg
     2401 agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     2461 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     2521 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     2581 agagcgcaga taccaaatac tgtt
//
