LOCUS       sequence-355546-        6725 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Punc-25::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans D-MN and 
            other neurals expression of miniSOG-SL2 RFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6725)
  AUTHORS   Gao S, Guan SA, Fouad AD, Meng J, Kawano T, Huang YC, Li Y, Alcaire 
            S, Hung W, Lu Y, Qi YB, Jin Y, Alkema M, Fang-Yen C, Zhen M
  TITLE     Excitatory motor neurons are local oscillators for backward 
            locomotion.
  JOURNAL   Elife. 2018 Jan 23;7. pii: 29915. doi: 10.7554/eLife.29915.
  PUBMED    29360035
REFERENCE   2  (bases 1 to 6725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-01-23"; volume: "7. pii: 29915. doi"; pages: " 
            10.7554/eLife.29915"
COMMENT     Sequence Label: pJH2844
FEATURES             Location/Qualifiers
     source          1..6725
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1978..2298
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     primer_bind     complement(4515..4537)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4637..4656
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4734..4752)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4820..4924
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4925..4993,4994..5785)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5143..5162)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5956..6544
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6445..6464
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6698..6715
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctgt
      241 aagtttaaac atgatcttac taactaacta ttctcattta aattttcaga gcttaaaaat
      301 ggctgaaatc actcacaacg atggatacgc taacaacttg gaaatgaaat aagcttgggc
      361 tgcagcatgc ggccgcgaaa tatcgatttt ttccgagata tctaaaattg aaagaaattt
      421 tcgattttct gagaaaaata cgaaaaaatc gaaaaaaaaa cccaaatttc ggttttttcg
      481 taaaaaaagt cgtaaaaatg taattttttt cctgaaaaat cggaattttt tacaaaatat
      541 cggaaaatac tcaaaaaaaa gctgaaaatt tcgattttcc ggagaaaaaa tctttttaaa
      601 aaaatatttt ttttttcaga aaataagaaa agccgaaatt tataactatt tctccggaaa
      661 ttcgaaattt ttaacgaaat atcggaataa tttttagatt tttcaagttt gactttgcga
      721 gaaaaaaatc ggaaaaacct cgattcgacg ccgaaaaatg ctccttttcg aaaagatttt
      781 tgaaatttca gaaaatcgac atgcaagcgc gctccacggc gaaatgacaa cgatgatcca
      841 ccgccctcaa aaagttgggt ctcgttaggt atttggcggt aaaactggta aaactccagt
      901 tttgcctcca acgagaccca attttttggg gcggtggtgg agcgcgcttg cacaagctga
      961 aagcattttt ctgcgactcg ataatatttt gaaaacctgt gtcaattctc gaaattcttt
     1021 ttttaaaaaa taatcccgag cttctctcag tcctcctcta tgaggatgtt cctttttttt
     1081 ggtttttcaa ttttttttaa aattccaaat ttctgttgtg caattcactt ccccccaaga
     1141 aatcccaaaa atccccagtt ttccccaaaa atgttccgtt ttcatgtgat ttttccccca
     1201 tttttaaaac atttttttga ctttttttta aaatgattat tattattgtt ttctatttca
     1261 tggccggtaa attatttttt ttctttcttt ttttttgctc ttttttttca agaattttcg
     1321 aattgtttga agggctgctc atctaatctt ttgtcatttt gtcctgatgc catcatttct
     1381 gagaggacct ttgaagactc gtcacgaaac gggagggggg ctcaagtgag cattattatt
     1441 attattattg tcgcaaaaag tttaccccgg gctccctctg gctcccctct ttgagcaagg
     1501 gtttaagggc tcattttgat gacgaattgc tcattgggat tatagtcacg cccctctttt
     1561 ggagcaacta cacaactgag ccacagtaat ccttgggggc ggggtcagta ggaccccctc
     1621 cggaataggg aaaagctcag ttcaccgcca aaaattggcc aaaggaccca aaggtatgtt
     1681 tcgaatgata ctaacataac atagaacatt ttcaggagga cccttggcta gcgtcgacgg
     1741 atcccgatgt cggacacaat tcttggtttc aacaaatcaa acgtcgtttt ggctgctgga
     1801 attgctggag ccgctttcct cggctactgc atttacttcg atcataagag aatcaacgct
     1861 ccagactaca aggacaagat taggcaaagt cagtgtttta acaacatatt tccttcggat
     1921 ttttatctaa aaacaactta ttttctttca gagagacgtg cccaggctgg ggatcctatg
     1981 gagaaaagtt tcgtgataac tgatccacgg ctgccagaca atcccatcat cttcgcatcc
     2041 gatggcttcc tggagctgac cgagtattcc agagaggaga tcctgggccg caatggccgc
     2101 tttctgcagg gaccagagac agaccaggcc acagtgcaga agattcgcga tgccattaga
     2161 gatcagcgcg agattaccgt gcagctgata aactacacaa aaagcgggaa gaaattctgg
     2221 aacctcctgc acctccagcc catgagggac cagaagggtg agctccagta tttcatcgga
     2281 gtgcagctgg atggataaag atccccgaga tcttcagcgc tcagcattcg tagaattgta
     2341 agtttaaact ttctcatact aactaacgcg ctctatttaa attttcagaa ttcgctgtct
     2401 catcctactt tcacctagtt aactgcttgt cttaaaatct atgcttctct ttagtatcta
     2461 aaattttcct agaagcttac aagtatataa atggtctctt ctcaataaag gttgtatatt
     2521 tattcatctt attgaatctg ccatttcctc gtttttgcga gtttatatac cttccaattt
     2581 tctttctatt gtattttcaa cttctaattt taattcaggg aaactgcttc aacgcatcgg
     2641 taccctatgg tctcaaaggg tgaagaagat aacatggcaa ttattaaaga gtttatgcgt
     2701 ttcaaggtgc atatggaggg atctgtcaat gggcatgagt ttgaaattga aggtgaagga
     2761 gaaggccgac catatgaggg aacacaaacc gcaaaactaa aggtaagttt aaacatatat
     2821 atactaacta accctgatta tttaaatttt caggtaacta aaggcggacc attaccattc
     2881 gcctgggaca tcctctctcc acagttcatg tatggaagta aagcttatgt taaacatccg
     2941 gcagatatac cagattattt gaaactttca ttcccggagg gttttaagtg ggaacgcgta
     3001 atgaattttg aagacggagg agttgttaca gtgacgcaag actcaaggta agtttaaaca
     3061 gttcggtact aactaaccat acatatttaa attttcagcc tccaagatgg agaatttatt
     3121 tataaagtca aacttcgagg aacgaatttc ccctcggatg gatctgttat gcagaagaag
     3181 actatgggat gggaagcttc aagtgaaaga atgtaccctg aagacggtgc tcttaaggga
     3241 gagattaaac aacgtcttaa attgaaagat ggaggacatt acgatgctga ggtaagttta
     3301 aacatgattt tactaactaa ctaatctgat ttaaattttc aggtgaagac aacttacaaa
     3361 gccaaaaaac cagttcagct gccaggagcg tacaatgtta atattaaact ggatatcacc
     3421 tcccacaacg aggattacac tatcgttgag caatatgaaa gagctgaagg gcggcactcg
     3481 acaggtggca tggatgaatt gtataagtag gagctcccga gatcttcagc gctcagcatt
     3541 cgtagaattg taagtttaaa ctttctcata ctaactaacg cgctctattt aaattttcag
     3601 aattccaact gagcgccggt cgctaccatt accaacttgt ctggtgtcaa aaataatagg
     3661 ggccgctgtc atcagagtaa gtttaaactg agttctacta actaacgagt aatatttaaa
     3721 ttttcagcat ctcgcgcccg tgcctctgac ttctaagtcc aattactctt caacatccct
     3781 acatgctctt tctccctgtg ctcccacccc ctatttttgt tattatcaaa aaacttctct
     3841 taatttcttt gttttttagc ttcttttaag tcacctctaa caatgaaatt gtgtagattc
     3901 aaaaatagaa ttaattcgta ataaaaagtc gaaaaaaatt gtgctccctc cccccattaa
     3961 taataattct atcccaaaat ctacacaatg ttctgtgtac acttcttatg ttttttactt
     4021 ctgataaatt tttttgaaac atcatagaaa aaaccgcaca caaaatacct tatcatatgt
     4081 tacgtttcag tttatgaccg caatttttat ttcttcgcac gtctgggcct ctcatgacgt
     4141 caaatcatgc tcatcgtgaa aaagttttgg agtatttttg gaatttttca atcaagtgaa
     4201 agtttatgaa attaattttc ctgcttttgc tttttggggt ttcccctatt gtttgtcaag
     4261 atttcgagga cggcgttttt cttgctaaaa tcacaagtat tgatgagcac gatgcaagaa
     4321 agatcggaag aaggtttggg tttgaggctc agtggaaggt gagtagaagt tgataatttg
     4381 aaagtggagt agtgtctatg gggtttttgc cttaaatgac agaatacatt cccaatatac
     4441 caaacataac tgtttcctac tagtcggccg tacgggccct ttcgtctcgc gcgtttcggt
     4501 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     4561 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     4621 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     4681 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcggcctta agggcctcgt
     4741 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     4801 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     4861 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     4921 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     4981 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     5041 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     5101 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     5161 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     5221 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     5281 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     5341 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     5401 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     5461 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     5521 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     5581 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     5641 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     5701 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     5761 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     5821 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     5881 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     5941 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     6001 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     6061 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     6121 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     6181 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     6241 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     6301 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     6361 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     6421 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     6481 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     6541 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     6601 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     6661 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     6721 ggaag
//
