LOCUS       sequence-355548-        7029 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PA-Flp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7029)
  AUTHORS   Jung H, Kim SW, Kim M, Hong J, Yu D, Kim JH, Lee Y, Kim S, Woo D, 
            Shin HS, Park BO, Do Heo W
  TITLE     Noninvasive optical activation of Flp recombinase for genetic 
            manipulation in deep mouse brain regions.
  JOURNAL   Nat Commun. 2019 Jan 18;10(1):314. doi: 10.1038/s41467-018-08282-8.
  PUBMED    30659191
REFERENCE   2  (bases 1 to 7029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-01-18"; volume: "10(1):314. doi"; pages: " 
            10.1038/s41467-018-08282-8"
COMMENT     Sequence Label: pAAV-EF1a-pMagHigh-FlpC
FEATURES             Location/Qualifiers
     source          1..7029
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   2..131
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        232..1410
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          463..1401
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1358..1378
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3157..3745
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3210..3230)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3628..3639)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3777..4253
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3976..3995)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4293..4433
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4293..4422
                     /label=AAV2 ITR
     rep_origin      4508..4963
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4595..4614)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4805..4826
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4980..4999)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5099..5121
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5159..5177)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5245..5349
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5350..5418,5419..6210)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5568..5587)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6381..6969
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6870..6889
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcctgcaggc agctgcgcgc tcgctcgctc actgaggccg cccgggcgtc gggcgacctt
       61 tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca actccatcac
      121 taggggttcc tgcggccgca cgcgtaagct ttgcaaagat ggataaagtt ttaaacagag
      181 aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt
      241 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
      301 ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
      361 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
      421 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt
      481 tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc
      541 tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg
      601 aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc
      661 tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag
      721 tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt
      781 cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc
      841 gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca
      901 ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg
      961 ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga
     1021 gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc
     1081 tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc
     1141 gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg
     1201 agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc
     1261 cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg
     1321 gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa
     1381 agtttttttc ttccatttca ggtgtcgtga ggtaccggat cctctagagt cgactccgga
     1441 accatgcaca ccctgtacgc ccccggcggc tacgacatca tgggctacct gagacagatc
     1501 agaaacagac ccaaccccca ggtggagctg ggccccgtgg acaccagctg cgccctgatc
     1561 ctgtgcgacc tgaagcagaa ggacaccccc atcgtgtacg ccagcgaggc cttcctgtat
     1621 atgaccggct acagcaacgc cgaggtgctg ggcagaaact gcagattcct gcagagcccc
     1681 gacggcatgg tgaagcccaa gagcaccaga aagtacgtgg acagcaacac catcaacacc
     1741 atcagaaagg ccatcgacag aaacgccgag gtgcaggtgg aggtggtgaa cttcaagaag
     1801 aacggccaga gattcgtgaa cttcctgacc atcatccccg tgagagacga gaccggcgag
     1861 tacagataca gcatgggctt ccagtgcgag accgagggtg gaggcggttc aggcggaggt
     1921 ggcagcggcg gtggcggatc gggcgagaag atcgccagct gtgccgccga gctgacctac
     1981 ctgtgctgga tgatcaccca caacggcacc gccatcaaga gggccacctt catgagctac
     2041 aacaccatca tcagcaacag cctgagcttc gacatcgtga acaagagcct gcagttcaag
     2101 tacaagaccc agaaggccac catcctggag gccagcctga agaagctgat ccccgcctgg
     2161 gagttcacca tcatccctta caacggccag aagcaccaga gcgacatcac cgacatcgtg
     2221 tccagcctgc agctgcagtt cgagagcagc gaggaggccg acaagggcaa cagccacagc
     2281 aagaagatgc tgaaggccct gctgtccgag ggcgagagca tctgggagat caccgagaag
     2341 atcctgaaca gcttcgagta caccagcagg ttcaccaaga ccaagaccct gtaccagttc
     2401 ctgttcctgg ccacattcat caactgcggc aggttcagcg acatcaagaa cgtggacccc
     2461 aagagcttca agctggtgca gaacaagtac ctgggcgtga tcattcagtg cctggtgacc
     2521 gagaccaaga caagcgtgtc caggcacatc tactttttca gcgccagagg caggatcgac
     2581 cccctggtgt acctggacga gttcctgagg aacagcgagc ccgtgctgaa gagagtgaac
     2641 aggaccggca acagcagcag caacaagcag gagtaccagc tgctgaagga caacctggtg
     2701 cgcagctaca acaaggccct gaagaagaac gccccctacc ccatcttcgc tatcaagaac
     2761 ggccctaaga gccacatcgg caggcacctg atgaccagct ttctgagcat gaagggcctg
     2821 accgagctga caaacgtggt gggcaactgg agcgacaaga gggcctccgc cgtggccagg
     2881 accacctaca cccaccagat caccgccatc cccgaccact acttcgccct ggtgtccagg
     2941 tactacgcct acgaccccat cagcaaggag atgatcgccc tgaaggacga gaccaacccc
     3001 atcgaggagt ggcagcacat cgagcagctg aagggcagcg ccgagggcag catcagatac
     3061 cccgcctgga acggcatcat cagccaggag gtgctggact acctgagcag ctacatcaac
     3121 aggcggatct aagaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt
     3181 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     3241 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     3301 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     3361 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     3421 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     3481 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     3541 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct atgttgccac ctggattctg
     3601 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     3661 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     3721 atctcccttt gggccgcctc cccgcatcga taccgagcgc tgctcgagag atctacgggt
     3781 ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc actccagtgc
     3841 ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag gtgtccttct
     3901 ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg aagacaacct
     3961 gtagggcctg cggggtctat tgggaaccaa gctggagtgc agtggcacaa tcttggctca
     4021 ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc gagttgttgg
     4081 gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag agacggggtt
     4141 tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac ccaccttggc
     4201 ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc cttctgattt
     4261 tgtaggtaac cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg agttggccac
     4321 tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc
     4381 gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct
     4441 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa
     4501 ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     4561 gtgaccgcta cacttgccag cgccttagcg cccgctcctt tcgctttctt cccttccttt
     4621 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     4681 cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt
     4741 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     4801 aatagtggac tcttgttcca aactggaaca acactcaact ctatctcggg ctattctttt
     4861 gatttataag ggattttgcc gatttcggtc tattggttaa aaaatgagct gatttaacaa
     4921 aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt
     4981 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     5041 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     5101 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     5161 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     5221 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     5281 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     5341 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     5401 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     5461 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     5521 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     5581 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     5641 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     5701 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     5761 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     5821 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     5881 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     5941 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     6001 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     6061 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     6121 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     6181 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     6241 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     6301 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     6361 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     6421 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     6481 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     6541 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     6601 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     6661 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     6721 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     6781 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     6841 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     6901 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     6961 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     7021 gctcacatg
//
