LOCUS       sequence-355549-       10429 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsTNRC6A-delMI-MII.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10429)
  AUTHORS   Lazzaretti D, Tournier I, Izaurralde E
  TITLE     The C-terminal domains of human TNRC6A, TNRC6B, and TNRC6C silence 
            bound transcripts independently of Argonaute proteins.
  JOURNAL   RNA. 2009 Jun . 15(6):1059-66.
  PUBMED    19383768
REFERENCE   2  (bases 1 to 10429)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2009 
            Jun . 15(6):1059-66."
COMMENT     Sequence Label: pCIneo-lambdaN-HA-HsTNRC6A-delMI-MII_F
FEATURES             Location/Qualifiers
     source          1..10429
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1089..1098
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1095..1160
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             1179..1205
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    5171..5182
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     polyA_signal    6133..6254
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6144..6163)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6198..6217
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      6440..6895
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6527..6546)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6737..6758
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7012..7369
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7012..7203
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7035..7055)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7220..7355
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7420..8214
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7474..7493)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8084..8103
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8278..8326
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(8367..8386)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8486..8508
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8546..8564)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8632..8736
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8737..8805,8806..9597)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8955..8974)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9768..10356
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10257..10276
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc caccatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag
     1141 ctcaatggaa agctgcaaac ccaccgctcg agatggctta cccatacgat gttccagatt
     1201 acgctatgga tgctgattct gcctccagtt ctgaatcaga gagaaacatc actatcatgg
     1261 cttcagggaa cacaggtggt gaaaaagatg gccttcggaa tagcactgga cttggttccc
     1321 aaaacaagtt tgtagttggt agcagcagca ataatgtggg ccatggaagt agtactgggc
     1381 catggggttt ttcccatgga gccataataa gcacatgtca ggtctctgtg gatgctcctg
     1441 aaagcaaatc tgaaagtagc aacaatagaa tgaatgcttg gggcactgta agttcttcat
     1501 caaatggagg gttaaatcca agcactttga attcagctag caaccatggt gcctggccag
     1561 tattagagaa caatggactt gccctaaaag ggcctgtagg gagtggtagt tctggcatta
     1621 atattcagtg cagtactata ggccagatgc ctaacaatca gagtattaac tctaaagtga
     1681 gtggtggttc tacccatggt acctggggaa gccttcagga aacttgtgaa tctgaagtaa
     1741 gtggtacaca gaaggtttca ttcagtggtc aacctcaaaa tattaccact gaaatgactg
     1801 gaccaaataa cactactaac tttatgacct ctagtttacc aaactccggt tcagtgcaga
     1861 ataatgagct gcctagtagt aacacagggg cctggcgtgt gagcacaatg aatcatcctc
     1921 agatgcaggc tccatcaggt atgaatggca cttccctttc tcaccttagc aatggagagt
     1981 caaaaagtgg aggctcttat ggtactacat ggggtgccta tggttctaat tactctggag
     2041 acaaatgttc aggccctaat ggccaagcta atggtgacac tgtgaatgca actctaatgc
     2101 agcctggcgt aaatggtcct atgggcacta actttcaagt taacacaaac aaaggaggtg
     2161 gtgtgtggga atctggtgca gcaaactccc agagtacatc atggggaagt ggaaatggca
     2221 caaattctgg aggaagtcga agaggatggg gaacccctgc acaaaacact ggcactaatt
     2281 tacccagcgt tgagtggaac aaactgccta gcaatcagca ttccaatgat agtgcaaatg
     2341 gcaatggtaa gacgtttaca aatggatgga aatctactga ggaagaggat cagggttctg
     2401 ccacatctca gacaaatgag caaagcagtg tgtgggccaa aacaggaggt acaatggaga
     2461 gcgatggtag tacagaaagc actggacgcc ttgaggaaaa aggaactggg gaaagtcaga
     2521 gtagagacag aagaaaaatt gatcagcaca cattactcca aagcattaat gggacagagg
     2581 cctggggaag ctctgcaaca cagactttta actcaggggc atgtatagat aagactagcc
     2641 ctaatggtaa tgatacctca tctgtatcag ggtggggcga tcccaaacct gctctgaggt
     2701 ggggagattc caaaggctca aactgccagg gggggtggga agatgattct gctgctacag
     2761 gaatggtcaa gagcaatcag tgggggaatt gcaaagagga gaaggctgca tggaatgact
     2821 cgcaaaagaa taaacaggga tggggtgatg gacaaaaatc aagccaaggg tggtctgttt
     2881 ctgccagtga taactgggga gaaacttcaa ggaataacca ttggggtgag gccaataaga
     2941 aatccagctc aggaggtagt gacagtgaca ggtccgtttc cggttggaac gaacttggta
     3001 aaactagttc tttcacttgg ggaaacaaca taaatccaaa taattcatca ggatgggatg
     3061 aatcttctaa acctactcct tcccagggat ggggagaccc tccaaagtct aatcagtctc
     3121 taggttgggg agattcgtca aagccagtca gctctccaga ctggaacaag caacaagaca
     3181 ttgttggatc ttggggaatc ccaccagcta caggcaaacc tcctggtaca ggctggctgg
     3241 ggggacctat accagcccca gcaaaagaag aagtcccaaa tggcaacagc cgttcagacc
     3301 agcaagcaca ggtacatcag ctgctaacgc ctgcaagtgc catctcaaac aaagaggcaa
     3361 gcagtggctc tggctggggt gagccctggg gggagccttc tactccagcc acaactgtgg
     3421 ataatggtac ttcagcatgg ggtaagccca tagacagtgg tcccagctgg ggggaaccca
     3481 ttgctgcggc atccagcaca tccacgtggg gctccagctc tgttggtcca caagcattaa
     3541 gcaaatctgg gccaaaatct atgcaagatg gctggtgtgg tgatgatatg ccattgcctg
     3601 gaaatcgccc cactggctgg gaagaggaag aggatgtgga gattggaatg tggaatagta
     3661 attcatctca agagcttaac tcatctttaa attggccacc atatacaaag aaaatgtcat
     3721 cgaagggtct gagtggcaaa aaaaggagaa gggaaggggg aatgatgaaa ggtggaaaca
     3781 aacaagaaga agcgtggata aatccatttg ttaaacagtt ttcaaacatc agtttttcga
     3841 gagactcacc agaggaaaat gtacaaagca ataagatgga cctttctgga ggaatgttac
     3901 aagacaaacg aatggagata gataaacata gcctaaatat tggtgattac aatcgaacgg
     3961 tcgggaaagg ccctggttct cggcctcaga tttccaaaga gtcttccatg gagcgcaatc
     4021 cttattttga taaggatggc attgtagcag atgaatccca aaacatgcag tttatgtcca
     4081 gtcaaagcat gaagcttccc ccttcaaata gtgcactacc taaccaggcc cttggctcca
     4141 tagcagggct gggtatgcaa aacttgaatt ctgttagaca gaatggcaat cccagtatgt
     4201 ttggtgttgg aaacacagca gcacaacccc ggggcatgca gcagcctcca gcacaacctc
     4261 ttagttcatc tcagcctaat ctccgtgctc aagtgcctcc tccattactc tcccctcagg
     4321 ttccagtttc attgctgaag tatgcaccaa acaacggtgg cctgaatcca ctctttggcc
     4381 ctcaacaggt agccatgctg aaccagctat cccagctaaa ccagctttct cagatctccc
     4441 agttacagcg attgttagcg cagcagcaaa gggcgcagag tcagagaagc gtgccttctg
     4501 ggaaccggcc gcagcaagac cagcagggtc gacctcttag tgtgcagcag caaatgatgc
     4561 aacaatctcg tcaacttgat ccaaacctgt tggtgaagca gcagactcca ccatctcagc
     4621 agcagccact ccatcagcca gccatgaagt ctttccttga caatgtcatg ccccacacta
     4681 cacctgagct gcaaaaaggg ccatcaccaa taaatgcttt cagcaacttc cctataggct
     4741 tgaactcaaa cttgaatgta aatatggata tgaacagtat taaagagcca cagtcaagac
     4801 taaggaagtg gacgacagtg ggcagcattt ctgtgaacac atctttggat caaaactcca
     4861 gcaaacatgg tgctatttca agtggtttca ggctggaaga gtctccattt gttccctatg
     4921 actttatgaa cagcagtact tcaccagcca gtcctccagg ttcaatagga gatggctggc
     4981 cacgtgccaa atcgcctaac ggctctagca gtgttaattg gccaccagaa tttcgtcctg
     5041 gtgagccatg gaaaggttat ccaaacattg accctgaaac tgacccttac gtcactcctg
     5101 gcagtgtcat aaacaatctt tcaattaata ctgtgcggga agttgaccac ctcagggaca
     5161 ggaacagtgg gtcatcctca tccttgaaca ccacgctgcc ttcaactagt gcctggtcat
     5221 ccattcgtgc ctccaactac aacgttcccc tcagcagtac agcacaaagc acttcagcca
     5281 gaaatagtga ttccaaattg acatggtctc ctggttcagt tacaaacacc tctctggctc
     5341 atgagctgtg gaaggtccct ttgccaccta aaaacatcac tgctccgtcc cgcccacctc
     5401 cgggactgac tggtcagaag ccacccttgt ctacgtggga taattctccc cttcgtatag
     5461 gtggaggatg gggaaattct gacgccagat ataccccagg ttccagctgg ggtgagagca
     5521 gctcagggag aataacaaat tggcttgttc taaaaaacct tacacctcag atcgatggct
     5581 caactctgcg cactctgtgc atgcagcacg gcccgctgat cacattccac ctgaacctcc
     5641 ctcacggaaa tgctctggtc cgctacagtt caaaagaaga ggtagtggag gcacaaaagt
     5701 ctctgcacat gtgtgtactg gggaacacta ctattcttgc tgagtttgcc agtgaagagg
     5761 agatcagtcg tttctttgca caaagccagt ctctgacccc ttctcccggc tggcagtctc
     5821 tcgggtccag ccagagccgg ctgggctccc tcgactgttc ccactcattc tccagccgga
     5881 ccgatctcaa tcactggaat ggtgctgggc tgtcgggaac taactgtgga gaccttcacg
     5941 gcacttcact ctgggggacc ccgcattatt ccacaagcct gtggggtccc ccaagcagca
     6001 gcgacccccg aggaattagc agcccatctc ccattaacgc ttttctttct gttgaccacc
     6061 tgggtggggg tggagagtcc atgtaagcgg ccgcttccct ttagtgaggg ttaatgcttc
     6121 gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     6181 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     6241 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggaga
     6301 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc cgataaggat
     6361 cgatccgggc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     6421 cagcctgaat ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     6481 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     6541 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     6601 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     6661 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     6721 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     6781 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     6841 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttcctga
     6901 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgc ggatctgcgc
     6961 agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct tctgaggcgg
     7021 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     7081 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     7141 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     7201 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     7261 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     7321 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttgattc
     7381 ttctgacaca acagtctcga acttaaggct agagccacca tgattgaaca agatggattg
     7441 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     7501 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     7561 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
     7621 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     7681 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     7741 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     7801 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     7861 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     7921 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
     7981 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     8041 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     8101 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     8161 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     8221 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga tggccgcaat
     8281 aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagcgata
     8341 aggatccgcg tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     8401 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     8461 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     8521 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     8581 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     8641 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     8701 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     8761 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     8821 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     8881 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     8941 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     9001 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     9061 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     9121 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     9181 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     9241 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     9301 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     9361 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     9421 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     9481 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     9541 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     9601 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     9661 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     9721 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     9781 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     9841 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     9901 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     9961 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
    10021 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
    10081 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
    10141 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
    10201 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
    10261 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
    10321 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
    10381 cggttcctgg ccttttgctg gccttttgct cacatggctc gacagatct
//
