LOCUS       sequence-355554-        7176 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Opto-cytTrkB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7176)
  AUTHORS   Hong J , Heo WD
  TITLE     Optogenetic Modulation of TrkB Signaling in the Mouse Brain.
  JOURNAL   J Mol Biol. 2020 Feb 14;432(4):815-827. doi: 
            10.1016/j.jmb.2020.01.010. Epub 2020 Jan 18.
  PUBMED    31962123
REFERENCE   2  (bases 1 to 7176)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2020.01.010"; journalName: "J Mol Biol"; date: 
            "2020-02-14- 14"; volume: "432"; issue: "4"; pages: "815-827"
COMMENT     Sequence Label: pAAV-CamK2a(0.4)-Opto-cytTrkB(E281A)-HA
FEATURES             Location/Qualifiers
     source          1..7176
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      533..542
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3251..3277
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    3305..3893
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3358..3378)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3776..3787)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3896..3912)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3897..3913)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    3923..4399
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4122..4141)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4439..4579
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4439..4568
                     /label=AAV2 ITR
     rep_origin      4654..5109
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4741..4760)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4951..4972
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5126..5145)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5245..5267
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5305..5323)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5391..5495
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5496..5564,5565..6356)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5714..5733)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6527..7115
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7016..7035
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtaacttg tggactaagt ttgttcacat ccccttctcc
      181 aaccccctca gtacatcacc ctgggggaac agggtccact tgctcctggg cccacacagt
      241 cctgcagtat tgtgtatata aggccagggc aaagaggagc aggttttaaa gtgaaaggca
      301 ggcaggtgtt ggggaggcag ttaccggggc aacgggaaca gggcgtttcg gaggtggttg
      361 ccatggggac ctggatgctg acgaaggctc gcgaggctgt gagcagccac agtgccctgc
      421 tcagaagccc caagctcgtc agtcaagccg gttctccgtt tgcactcagg agcacgggca
      481 ggcgagtggc ccctagttct gggggcagct ctagagccga tccccgggta ccgccaccat
      541 gggatgtata aaatcaaaag ggaaagacag cgcgggagca gatagtgctg gtagtgctgg
      601 tagtgctggg aattccaagt tggcaagaca ctccaagttt ggcatgaaag gcccagcttc
      661 cgtcatcagc aacgacgatg actctgccag ccctctccac cacatctcca acgggagcaa
      721 cactccgtct tcttcggagg gcgggcccga tgctgtcatc attgggatga ccaagatccc
      781 tgtcattgaa aacccccagt acttcggtat caccaacagc cagctcaagc cggacacatt
      841 tgttcagcac atcaagagac acaacatcgt tctgaagagg gagcttggag aaggagcctt
      901 tgggaaagtt ttcctagcgg agtgctataa cctctgcccc gagcaggata agatcctggt
      961 ggccgtgaag acgctgaagg acgccagcga caatgctcgc aaggactttc atcgcgaagc
     1021 cgagctgctg accaacctcc agcacgagca cattgtcaag ttctacggtg tctgtgtgga
     1081 gggcgaccca ctcatcatgg tctttgagta catgaagcac ggggacctca acaagttcct
     1141 tagggcacac gggccagatg cagtgctgat ggcagagggt aacccgccca ccgagctgac
     1201 gcagtcgcag atgctgcaca tcgctcagca aatcgcagca ggcatggtct acctggcatc
     1261 ccaacacttc gtgcaccgag acctggccac ccggaactgc ttggtaggag agaacctgct
     1321 ggtgaaaatt ggggacttcg ggatgtcccg ggatgtatac agcaccgact actaccgggt
     1381 tggtggccac acaatgttgc ccatccgatg gatgcctcca gagagcatca tgtacaggaa
     1441 attcaccacc gagagtgacg tctggagcct gggagttgtg ttgtgggaga tcttcaccta
     1501 cggcaagcag ccctggtatc agctatcaaa caacgaggtg atagaatgca tcacccaggg
     1561 cagagtcctt cagcggcctc gcacgtgtcc ccaggaggtg tacgagctga tgctgggatg
     1621 ctggcagcgg gaaccacaca caaggaagaa catcaagaac atccacacac tccttcagaa
     1681 cttggcgaag gcgtcgcccg tctacctgga cattctaggc gcggatccga caggtatgaa
     1741 gatggacaaa aagaccatcg tctggtttcg gagagatttg agaatagaag ataatcccgc
     1801 gctcgccgcc gcggcccacg agggttccgt cttccccgtt ttcatttggt gtcctgaaga
     1861 agaaggccag ttttatcccg gaagggcctc taggtggtgg atgaagcaaa gtctggccca
     1921 tcttagccag tcactgaaag cactgggcag tgatcttacc ctgatcaaga cacacaatac
     1981 catctctgcc atcctcgact gcatcagggt gaccggcgca acgaaagtcg tgtttaacca
     2041 cctgtacgat ccagttagtc tggtgcgcga ccacactgtg aaggagaagc tggtggaacg
     2101 ggggatcagt gtgcagagct acaacgggga ccttctgtac gagccatggg agatctattg
     2161 cgagaaaggg aaaccgttca cctccttcaa cagttactgg aagaaatgtt tggatatgtc
     2221 aatagagtcc gttatgttgc cccctccctg gagactgatg ccgattactg ctgctgcaga
     2281 ggccatctgg gcctgctcca tcgaggaact cggtctggaa aatgaagcag aaaagccaag
     2341 caatgcactt ctcactagag cctggagccc cggctggtct aatgccgaca agctgcttaa
     2401 cgagttcatc gaaaaacaac tgattgacta cgcgaagaac tccaagaaag tggtaggtaa
     2461 ctcaactagc ttgctctctc catatctcca ttttggcgag atttctgtcc gccatgtatt
     2521 tcagtgcgct cggatgaaac agattatctg ggctcgcgat aaaaacagcg aaggcgccga
     2581 aagcgccgat ctgttcctgc gagggatcgg acttcgggaa tactcccggt atatatgttt
     2641 caactttcca ttcacacacg agcagagtct gttgtcccac ctcaggttct tcccctggga
     2701 cgccgatgtc gacaaattca aggcatggag acagggaagg acagggtacc cactcgtgga
     2761 tgctggcatg agagagctct gggctacagg ctggatgcac aaccgcatcc gggtaatcgt
     2821 gtcctcattt gctgtcaagt ttctgctcct gccttggaaa tggggaatga agtacttttg
     2881 ggataccctt ctcgacgccg acttggagtg tgacattctg ggatggcaat atattagcgg
     2941 gtcaattcct gacggccatg agttggacag gttggacaat ccggccttgc agggagctaa
     3001 gtatgatccc gaaggagagt atattcgaca gtggctcccc gagctggccc gacttcctac
     3061 ggagtggatt caccatcctt gggacgcacc actgacagtg ctcaaggcaa gcggggtgga
     3121 gctgggcacc aattacgcta agcctatagt tgatatagat acagcacgcg agctgctggc
     3181 taaagcgatc tctcgcactc gggaggcgca gattatgatc ggtgctgccg gcagcggcag
     3241 cccaccggta tatccatatg atgttccaga ttatgcttaa gaattcgata tcaagcttat
     3301 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     3361 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     3421 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     3481 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     3541 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     3601 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     3661 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     3721 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     3781 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     3841 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
     3901 ccgtcgacct cgagagatct acgggtggca tccctgtgac ccctccccag tgcctctcct
     3961 ggccctggaa gttgccactc cagtgcccac cagccttgtc ctaataaaat taagttgcat
     4021 cattttgtct gactaggtgt ccttctataa tattatgggg tggagggggg tggtatggag
     4081 caaggggcaa gttgggaaga caacctgtag ggcctgcggg gtctattggg aaccaagctg
     4141 gagtgcagtg gcacaatctt ggctcactgc aatctccgcc tcctgggttc aagcgattct
     4201 cctgcctcag cctcccgagt tgttgggatt ccaggcatgc atgaccaggc tcagctaatt
     4261 tttgtttttt tggtagagac ggggtttcac catattggcc aggctggtct ccaactccta
     4321 atctcaggtg atctacccac cttggcctcc caaattgctg ggattacagg cgtgaaccac
     4381 tgctcccttc cctgtccttc tgattttgta ggtaaccacg tgcggaccga gcggccgcag
     4441 gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
     4501 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
     4561 gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     4621 atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg
     4681 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg
     4741 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     4801 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     4861 aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     4921 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     4981 tcaactctat ctcgggctat tcttttgatt tataagggat tttgccgatt tcggtctatt
     5041 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt
     5101 ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     5161 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     5221 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     5281 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     5341 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     5401 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     5461 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     5521 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     5581 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     5641 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     5701 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     5761 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     5821 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     5881 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     5941 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     6001 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     6061 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     6121 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     6181 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     6241 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     6301 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     6361 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     6421 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     6481 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     6541 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     6601 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     6661 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     6721 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     6781 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     6841 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     6901 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     6961 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     7021 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     7081 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     7141 ggttcctggc cttttgctgg ccttttgctc acatgt
//
