LOCUS       sequence-355560-        8386 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmAGO1-F594V.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8386)
  AUTHORS   Eulalio A, Helms S, Fritzsch C, Fauser M, Izaurralde E
  TITLE     A C-terminal silencing domain in GW182 is essential for miRNA 
            function.
  JOURNAL   RNA. 2009 Jun;15(6):1067-77. doi: 10.1261/rna.1605509. Epub 2009 Apr
            21.
  PUBMED    19383769
REFERENCE   2  (bases 1 to 8386)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.1605509"; journalName: "RNA"; date: "2009-06"; volume: 
            "15"; issue: "6"; pages: "1067-77"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmAGO1-F594V-siRNAres_F
FEATURES             Location/Qualifiers
     source          1..8386
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..2510
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2531..2596
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2627..2653
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5637..5678
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5688..5705
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5728..5745)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5778..5912
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5802..5821)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5856..5875
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6513..7101)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6593..6612)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7272..8063,8064..8132))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7895..7914
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8133..8237)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8305..8323
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 catgaatggc atcaactctg aatcaaatct ttgcagatgc acctacttct catttccact
       61 gtcacatcat ttttccagat ctcgctgcct gttatgtggc ccacaaacca agacacgttt
      121 tatggccatt aaagctggct gatcgtcgcc aaacaccaaa tacataatga atatgtacac
      181 attcgagaaa gaagcgatca aagaagcgtc ttcgggcgga gtaggagaat gcggaggaga
      241 aggagaacga gctgatctag tatctctcca caatccaatg ccaactgacc aactggccat
      301 attcggagca atttgaagcc aatttccatc gcctggcgat cgctccattc ttggctatat
      361 gtttttcacc gttacccggg gccattttca aagactcgtc ggcaagataa gattgtgtca
      421 ctcgctgtct ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag
      481 tattttgaag aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac
      541 aaaaacatgt tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt
      601 atgtatatac ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac
      661 tgatgatatt ttaagatgat gccagaccaa aaggcttgaa tttctgcgtc ttttgccgaa
      721 cgcagtgcat gtgcaattgt tgttttttgg aatattcaat tttcggactg tccgctttga
      781 tttcagtttc ttggcttatt caaaaagcaa agtaaagcca aaaaagcgag atggcaatac
      841 caaatgcggc aaaacggtag tggaaggaaa ggggtgcggg gcagcggaag gaagggtggg
      901 gcggggcgtg gcggggtctg tggctgggcg cgacgtcacc gacgttggag ccactccttt
      961 gaccatgtgt gcgtgtgtgt attattcgtg tctcgccact cgccggttgt ttttttcttt
     1021 ttatgctgcg ctctctctag cgccatctcg cttacgcatg ctcaacgcac cgcatgttgc
     1081 cgtttccttt tatgcgtcat tttggctcga aataggcaat tatttaaaca aagattagtc
     1141 aacgaaaacg ctaaaataaa taagtctaca atatggttac ttattgccat gtgtgtgcag
     1201 ccaacgatag caacaaaagc aacaacacag gtggctttcc ctctttcact ttttgtttgc
     1261 aagccgcgtg cgagcaagac ggcacgaccg gcaaacgcaa ttacgctgac aaagagcaga
     1321 cgaagttttg gcgaaaaaca tcaaggcgcc tgatacgaat gcatttgcaa taacaattgc
     1381 gatatttaat attgtttatg aagctgtttg acttcaaaac acacaaaaaa aaaaataaaa
     1441 caaattattt gaaagagaat taggaatcgg acgcttatcg ttagggtaac aacaagaaat
     1501 gcttactgag tcacagcctc tggaaaactg ccgcaagcca gagagagaga gaaaaagagg
     1561 gagagcagct tagaccgcat gtgcttgtgt gtgaggcgtc tctctcttcg tctctgttgc
     1621 gcaaacgcat agactgcact gaaaaaatcg attacctatt ttttatgaat gaatatttgc
     1681 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1741 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1801 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1861 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgaatgcc ctactagaag
     1921 atgtgtgttg cacaaaatgt ccctggaata accaatttga agtgcagata gcagtaaacg
     1981 taagctaata tgaatattat ttaactgtaa tgttttaata tcgctggaca ttactaataa
     2041 acccactata aacacatgta catatgtatg ttttggcata caatgagtag ttggggaaaa
     2101 aatgtgtaaa agcaccgtga ccatcacagc ataaagataa ccagctgaag tatcgaatat
     2161 gagtaacccc caaattgaat cacatgccgc aactgatagg acccatggaa gtacactctt
     2221 catggcgata tacaagacac acacaagcac gaacacccag ttgcggagga aattctccgt
     2281 aaatgaaaac ccaatcggcg aacaattcat acccatatat ggtaaaagtt ttgaacgcga
     2341 cttgagagcg gagagcattg cggctgataa ggttttagcg ctaagcgggc tttataaaac
     2401 gggctgcggg accagttttc atatcactac cgtttgagtt cttgtgctgt gtggatactc
     2461 ctcccgacac aaagccgctc catcagccag cagtcgtcta atccagagac cccggatcgg
     2521 ggtacccaac atggacgcac aaacacgacg acgtgagcgt cgcgctgaga aacaagctca
     2581 atggaaagct gcaaacggtg gatctcctcc aagcttgaat tctatgtacc catacgatgt
     2641 tccagattac gctatgtcca cggagcgtga gctggctccc ggtgggccag ctcagctcca
     2701 tccgcacacg ctgccgctga cttttccgga tctccagatg acctccaccg tgggcatcat
     2761 tgggaaagtc tacgagtcac agtggacccc ctcgcccact cggcctcaga gtccctctca
     2821 ggcgcagact agcttcgaca cgctcacatc accaccagcg cccggctcgt cggtcaatcc
     2881 caccgccgtc accagcccaa gtgcccagaa tgtggccgct ggtggagcaa ctgtggccgg
     2941 tgcagcagca actgccgccc aggtggcctc cgccttgggt gccaccaccg gcagcgtgac
     3001 gccagcaatt gccaccgcaa cgccagccac gcagccggat atgcctgtct ttacgtgtcc
     3061 acgacgcccg aatctcggac gagagggtcg cccgattgtg ctgcgcgcca atcacttcca
     3121 ggtgacaatg ccgcgtggct atgtgcatca ctatgacatc aatatacagc cggacaagtg
     3181 tccgcgaaag gtgaaccgtg agattatcga gactatggtg catgcctata gcaagatctt
     3241 cggagtgctc aagccggtgt tcgatggtcg caacaatctg tatacccgcg atcccctgcc
     3301 cattggcaac gagcgtctag agctggaggt tactctaccc ggcgagggca aagatcgaat
     3361 ctttcgcgta acgatcaagt ggcaggctca ggtctcgctc ttcaatttgg aggaagctct
     3421 cgaaggccgc acgcggcaga taccctatga tgccattttg gcgctcgatg tggtcatgcg
     3481 ccatctgccc agcatgacgt acacgccagt gggacgtagc ttcttcagtt ccccggaggg
     3541 ttactaccat cccctgggtg gtggacgcga ggtttggttc ggtttccatc agagcgcaag
     3601 gccctcgcag tggaagatga tgctcaatat cgatgtctcg gccaccgctt tctacaaggc
     3661 tcaaccagtc attgacttca tgtgcgaggt gctggacatt cgcgacatca acgagcagcg
     3721 caaaccgctc accgattcgc agcgcgtcaa gttcaccaaa gaaattaaag gcctgaagat
     3781 cgagatcacc cactgcggcc agatgcgtcg caagtatcgt gtgtgcaacg tcactcgccg
     3841 ccccgctcag atgcaatcat tcccactgca gctggagaac ggacagaccg tagagtgcac
     3901 cgtggccaag tacttcctgg acaagtaccg catgaaattg cgctacccgc acttgccctg
     3961 cctgcaggtt ggccaagagc acaagcacac ttacctgcct ctagaggtgt gcaacattgt
     4021 ggccggacag cggtgcatta aaaagctgac cgatatgcag acgtcgacca tgatcaaggc
     4081 cacagctcgt tcagctccgg atcgtgagcg tgagattaac aacttggtaa agcgcgccga
     4141 cttcaacaac gattcgtatg tgcaagagtt tggcctgacc atctccaatt cgatgatgga
     4201 ggtacgagga cgcgtcttgc ctcctcccaa gcttcagtat gggggacgtg tgtctaccgg
     4261 cctcaccggc cagcagctgt tcccgccaca gaacaaggtg agcttggcct cgcccaacca
     4321 gggtgtatgg gatatgcgag gcaagcagtt cttcactggc gtcgagatcc gcatctgggc
     4381 catcgcctgt tttgccccac agcgcacggt gcgcgaggat gcgctgcgta atgtcaccca
     4441 gcagctgcag aagatctcaa acgatgcagg catgccgata attggacagc cgtgcttctg
     4501 taagtacgcc accgggccgg atcaagtgga acccatgttc cgttacctga agatcacctt
     4561 ccccggcctg caactcgtcg tggttgtgct gcccggcaag actccagtat acgccgaggt
     4621 gaagcgtgta ggtgacaccg ttctgggtat ggccacccag tgtgtgcagg ccaagaacgt
     4681 gaacaagaca tcgccacaga cgctctctaa tctgtgtctg aagatcaacg tcaagttggg
     4741 cggcatcaat tcaattctgg tcccctccat tcggccaaag gtcttcaatg agccggtcat
     4801 ctttttgggt gccgatgtga cacacccacc agctggcgac aacaagaaac catcgattgc
     4861 cgccgtcgtg ggctccatgg atgcccatcc atcgcggtat gccgccaccg ttcgcgtaca
     4921 gcagcaccga caggagatca tccaggagct gagcagcatg gtgcgcgagc tgttgatcat
     4981 gttctacaag tcgacgggcg gctacaagcc ccaccgcatc atactctatc gtgacggagt
     5041 ctccgaggga caattcccac atgtcctgca acacgaattg accgccattc gcgaggcctg
     5101 cattaagcta gagccagagt atcggccggg catcacattc attgtggtgc agaagcgcca
     5161 tcacactcga ctcttctgcg cggagaagaa ggagcagagc ggcaaatcgg gcaatattcc
     5221 cgcaggcacc accgtcgatg tgggcatcac acatcccacc gaatttgatt tctatctatg
     5281 cagccatcag ggcatccagg gcaccagtcg cccctcgcac taccacgttc tgtgggacga
     5341 caatcacttt gactcggacg agctgcagtg cctcacgtat cagctatgcc gtacgtacgt
     5401 gcgctgcacc cgatccgtca gtataccggc gccagcctac tacgcccatt tagtggcctt
     5461 ccgtgccaga tatcatctgg tggagaagga gcacgattcg ggcgagggtt cgcaccagag
     5521 cggctgctca gaggatcgta cgccaggtgc catggccagg gccatcactg tgcacgcgga
     5581 taccaagaag gtcatgtact ttgcctaact cgagtctaga gggcccgcgg ttcgaaggta
     5641 agcctatccc taaccctctc ctcggtctcg attctacgcg taccggtcat catcaccatc
     5701 accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctaaggcc tgagctcgct
     5761 gatcagcctc gatcgaggat ccagacatga taagatacat tgatgagttt ggacaaacca
     5821 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     5881 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     5941 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
     6001 gtatggctga ttatgatcag tcgaccgatg cccttgagag ccttcaaccc agtcagctcc
     6061 ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg
     6121 caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc
     6181 tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct
     6241 caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc
     6301 ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg
     6361 gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc
     6421 atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggcca gcaaaaggcc
     6481 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     6541 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     6601 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     6661 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     6721 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     6781 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     6841 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     6901 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     6961 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     7021 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     7081 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     7141 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     7201 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     7261 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     7321 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     7381 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     7441 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     7501 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     7561 agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt
     7621 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     7681 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     7741 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     7801 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     7861 cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact
     7921 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     7981 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     8041 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     8101 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     8161 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     8221 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     8281 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa
     8341 ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaaat
//
