LOCUS       sequence-355561-        7554 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Opto-cytTrkB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7554)
  AUTHORS   Hong J , Heo WD
  TITLE     Optogenetic Modulation of TrkB Signaling in the Mouse Brain.
  JOURNAL   J Mol Biol. 2020 Feb 14;432(4):815-827. doi: 
            10.1016/j.jmb.2020.01.010. Epub 2020 Jan 18.
  PUBMED    31962123
REFERENCE   2  (bases 1 to 7554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2020.01.010"; journalName: "J Mol Biol"; date: 
            "2020-02-14- 14"; volume: "432"; issue: "4"; pages: "815-827"
COMMENT     Sequence Label: pAAV-hSyn1-DIO-Opto-cytTrkB(E281A)-HA
FEATURES             Location/Qualifiers
     source          1..7554
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   2..131
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        176..623
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     protein_bind    complement(661..694)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(745..778)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(790..816)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      3525..3534
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    3540..3573
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    3624..3657
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    3682..4270
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3735..3755)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4153..4164)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4302..4778
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4501..4520)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4818..4958
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4818..4947
                     /label=AAV2 ITR
     rep_origin      5033..5488
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5120..5139)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5330..5351
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5505..5524)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5624..5646
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5684..5702)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5770..5874
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5875..5943,5944..6735)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6093..6112)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6906..7494
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7395..7414
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcctgcaggc agctgcgcgc tcgctcgctc actgaggccg cccgggcgtc gggcgacctt
       61 tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca actccatcac
      121 taggggttcc tgcggccgca cgcgtgtgta tagactgcag agggccctgc gtatgagtgc
      181 aagtgggttt taggaccagg atgaggcggg gtgggggtgc ctacctgacg accgaccccg
      241 acccactgga caagcaccca acccccattc cccaaattgc gcatccccta tcagagaggg
      301 ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca ccgcggacag
      361 tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa ggcgcgctga
      421 cgtcactcgc cggtcccccg caaactcccc ttcccggcca ccttggtcgc gtccgcgccg
      481 ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac gggcgcgacc
      541 atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc agcggaggag
      601 tcgtgtcgtg cctgagagcg cagtcgagaa ggtaccggaa cctctagagt cgactccgga
      661 ataacttcgt ataggatact ttatacgaag ttatgcagaa tggtagctgg attgtagctg
      721 ctattagcaa tatgaaacct cttaataact tcgtatagca tacattatac gaagttatgg
      781 cgcgccttaa gcataatctg gaacatcata tggatatacc ggtgggctgc cgctgccggc
      841 agcaccgatc ataatctgcg cctcccgagt gcgagagatc gctttagcca gcagctcgcg
      901 tgctgtatct atatcaacta taggcttagc gtaattggtg cccagctcca ccccgcttgc
      961 cttgagcact gtcagtggtg cgtcccaagg atggtgaatc cactccgtag gaagtcgggc
     1021 cagctcgggg agccactgtc gaatatactc tccttcggga tcatacttag ctccctgcaa
     1081 ggccggattg tccaacctgt ccaactcatg gccgtcagga attgacccgc taatatattg
     1141 ccatcccaga atgtcacact ccaagtcggc gtcgagaagg gtatcccaaa agtacttcat
     1201 tccccatttc caaggcagga gcagaaactt gacagcaaat gaggacacga ttacccggat
     1261 gcggttgtgc atccagcctg tagcccagag ctctctcatg ccagcatcca cgagtgggta
     1321 ccctgtcctt ccctgtctcc atgccttgaa tttgtcgaca tcggcgtccc aggggaagaa
     1381 cctgaggtgg gacaacagac tctgctcgtg tgtgaatgga aagttgaaac atatataccg
     1441 ggagtattcc cgaagtccga tccctcgcag gaacagatcg gcgctttcgg cgccttcgct
     1501 gtttttatcg cgagcccaga taatctgttt catccgagcg cactgaaata catggcggac
     1561 agaaatctcg ccaaaatgga gatatggaga gagcaagcta gttgagttac ctaccacttt
     1621 cttggagttc ttcgcgtagt caatcagttg tttttcgatg aactcgttaa gcagcttgtc
     1681 ggcattagac cagccggggc tccaggctct agtgagaagt gcattgcttg gcttttctgc
     1741 ttcattttcc agaccgagtt cctcgatgga gcaggcccag atggcctctg cagcagcagt
     1801 aatcggcatc agtctccagg gagggggcaa cataacggac tctattgaca tatccaaaca
     1861 tttcttccag taactgttga aggaggtgaa cggtttccct ttctcgcaat agatctccca
     1921 tggctcgtac agaaggtccc cgttgtagct ctgcacactg atcccccgtt ccaccagctt
     1981 ctccttcaca gtgtggtcgc gcaccagact aactggatcg tacaggtggt taaacacgac
     2041 tttcgttgcg ccggtcaccc tgatgcagtc gaggatggca gagatggtat tgtgtgtctt
     2101 gatcagggta agatcactgc ccagtgcttt cagtgactgg ctaagatggg ccagactttg
     2161 cttcatccac cacctagagg cccttccggg ataaaactgg ccttcttctt caggacacca
     2221 aatgaaaacg gggaagacgg aaccctcgtg ggccgcggcg gcgagcgcgg gattatcttc
     2281 tattctcaaa tctctccgaa accagacgat ggtctttttg tccatcttca tacctgtcgg
     2341 atccgcgcct agaatgtcca ggtagacggg cgacgccttc gccaagttct gaaggagtgt
     2401 gtggatgttc ttgatgttct tccttgtgtg tggttcccgc tgccagcatc ccagcatcag
     2461 ctcgtacacc tcctggggac acgtgcgagg ccgctgaagg actctgccct gggtgatgca
     2521 ttctatcacc tcgttgtttg atagctgata ccagggctgc ttgccgtagg tgaagatctc
     2581 ccacaacaca actcccaggc tccagacgtc actctcggtg gtgaatttcc tgtacatgat
     2641 gctctctgga ggcatccatc ggatgggcaa cattgtgtgg ccaccaaccc ggtagtagtc
     2701 ggtgctgtat acatcccggg acatcccgaa gtccccaatt ttcaccagca ggttctctcc
     2761 taccaagcag ttccgggtgg ccaggtctcg gtgcacgaag tgttgggatg ccaggtagac
     2821 catgcctgct gcgatttgct gagcgatgtg cagcatctgc gactgcgtca gctcggtggg
     2881 cgggttaccc tctgccatca gcactgcatc tggcccgtgt gccctaagga acttgttgag
     2941 gtccccgtgc ttcatgtact caaagaccat gatgagtggg tcgccctcca cacagacacc
     3001 gtagaacttg acaatgtgct cgtgctggag gttggtcagc agctcggctt cgcgatgaaa
     3061 gtccttgcga gcattgtcgc tggcgtcctt cagcgtcttc acggccacca ggatcttatc
     3121 ctgctcgggg cagaggttat agcactccgc taggaaaact ttcccaaagg ctccttctcc
     3181 aagctccctc ttcagaacga tgttgtgtct cttgatgtgc tgaacaaatg tgtccggctt
     3241 gagctggctg ttggtgatac cgaagtactg ggggttttca atgacaggga tcttggtcat
     3301 cccaatgatg acagcatcgg gcccgccctc cgaagaagac ggagtgttgc tcccgttgga
     3361 gatgtggtgg agagggctgg cagagtcatc gtcgttgctg atgacggaag ctgggccttt
     3421 catgccaaac ttggagtgtc ttgccaactt ggaattccca gcactaccag cactaccagc
     3481 actatctgct cccgcgctgt ctttcccttt tgattttata catcccatgg tggctagcaa
     3541 taacttcgta taaagtatcc tatacgaagt tatttgcctt aacccagaaa ttatcactgt
     3601 tattctttag aatggtgcaa agaataactt cgtataatgt atgctatacg aagttatgaa
     3661 ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact
     3721 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     3781 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     3841 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     3901 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     3961 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     4021 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     4081 tcgtcctttc cttggctgct cgcctatgtt gccacctgga ttctgcgcgg gacgtccttc
     4141 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     4201 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     4261 gcctccccgc atcgataccg agcgctgctc gagagatcta cgggtggcat ccctgtgacc
     4321 cctccccagt gcctctcctg gccctggaag ttgccactcc agtgcccacc agccttgtcc
     4381 taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat attatggggt
     4441 ggaggggggt ggtatggagc aaggggcaag ttgggaagac aacctgtagg gcctgcgggg
     4501 tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca atctccgcct
     4561 cctgggttca agcgattctc ctgcctcagc ctcccgagtt gttgggattc caggcatgca
     4621 tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc atattggcca
     4681 ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc aaattgctgg
     4741 gattacaggc gtgaaccact gctcccttcc ctgtccttct gattttgtag gtaaccacgt
     4801 gcggaccgag cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc
     4861 tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc
     4921 ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct
     4981 ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc
     5041 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     5101 gccagcgcct tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     5161 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     5221 cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc
     5281 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     5341 ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt
     5401 ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     5461 tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat
     5521 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     5581 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     5641 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     5701 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     5761 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     5821 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     5881 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     5941 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     6001 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     6061 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     6121 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     6181 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     6241 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     6301 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     6361 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     6421 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     6481 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     6541 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     6601 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     6661 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     6721 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     6781 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     6841 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     6901 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     6961 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     7021 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     7081 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     7141 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     7201 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     7261 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     7321 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     7381 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     7441 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     7501 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catg
//
