LOCUS       sequence-355569-        6822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  injections to make Bmp reporter mouse transgenic line.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6822)
  AUTHORS   Ivanovitch K, Soro-Barrio P, Chakravarty P, Jones RA, Bell DM, 
            Mousavy Gharavy SN, Stamataki D, Delile J, Smith JC, Briscoe J
  TITLE     Ventricular, atrial, and outflow tract heart progenitors arise from 
            spatially and molecularly distinct regions of the primitive streak.
  JOURNAL   PLoS Biol. 2021 May 17;19(5):e3001200. doi: 
            10.1371/journal.pbio.3001200. eCollection 2021 May.
  PUBMED    33999917
REFERENCE   2  (bases 1 to 6822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2021-05-17"; volume: "19(5):e3001200. doi"; pages: " 
            10.1371/journal.pbio.3001200. eCollection 2021 May"
COMMENT     Sequence Label: pJC5-4-insulator-BREreporter-insulator
FEATURES             Location/Qualifiers
     source          1..6822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(25..42)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(25..41)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(34..56)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(250..269)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     369..391
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(429..447)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        515..619
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(620..688,689..1480)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(838..857)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1651..2239
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2140..2159
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2393..2410
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2527..2548
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2563..2568,2569..2586,2587..2593)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2601..2617
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2606..2628
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2625..2641
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2625..2641
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2660..2679
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2660..2678
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4112..4489
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      4502..4511
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4508..4510,4511..4513,4514..5227)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4553..4574)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4814..4833)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5161..5182
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5267..5388
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5278..5297)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5332..5351
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tatagtgtca cctaaatcca attcactggc cgtcgtttta caacgtcgtg actgggaaaa
       61 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
      121 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
      181 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
      241 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
      301 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
      361 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
      421 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
      481 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
      541 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
      601 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
      661 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
      721 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
      781 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
      841 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
      901 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
      961 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     1021 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     1081 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     1141 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     1201 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     1261 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     1321 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     1381 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     1441 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     1501 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     1561 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     1621 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     1681 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     1741 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     1801 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1861 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1921 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1981 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     2041 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     2101 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2161 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2221 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     2281 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     2341 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     2401 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     2461 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     2521 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     2581 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     2641 ccatgattac gccaagctct aatacgactc actataggga gacaagcttg catgcctgca
     2701 ggtcgactct agagggacag ccccccccca aagcccccag ggatgtaatt acgtccctcc
     2761 cccgctaggg ggcagcagcg agccgcccgg ggctccgctc cggtccggcg ctccccccgc
     2821 atccccgagc cggcagcgtg cggggacagc ccgggcacgg ggaaggtggc acgggatcgc
     2881 tttcctctga acgcttctcg ctgctctttg agcctgcaga cacctggggg gatacgggga
     2941 aaaagcttta ggctgaaaga gagatttaga atgacagaat catagaacgg cctgggttgc
     3001 aaaggagcac agtgctcatc cagatccaac cccctgctat gtgcagggtc atcaaccagc
     3061 agcccaggct gcccagagcc acatccagcc tggccttgaa tgcctgcagg gatggggcat
     3121 ccacagcctc cttgggcaac ctgttcagtg cgtcaccacc ctctggggga aaaactgcct
     3181 cctcatatcc aacccaaacc tcccctgtct cagtgtaaag ccattccccc ttgtcctatc
     3241 aagggggagt ttgctgtgac attgttggtc tggggtgaca catgtttgcc aattcagtgc
     3301 atcacggaga ggcagatctt ggggataagg aagtgcagga cagcatggac gtgggacatg
     3361 caggtgttga gggctctggg acactctcca agtcacagcg ttcagaacag ccttaaggat
     3421 aagaagatag gatagaagga caaagagcaa gttaaaaccc agcatggaga ggagcacaaa
     3481 aaggccacag acactgctgg tccctgtgtc tgagcctgca tgtttgatgg tgtctggatg
     3541 caagcagaag gggtggaaga gcttgcctgg agagatacag ctgggtcagt aggactggga
     3601 caggcagctg gagaattgcc atgtagatgt tcatacaatc gtcaaatcat gaaggctgga
     3661 aaagccctcc aagatcccca agaccaaccc caacccaccc accgtgccca ctggccatgt
     3721 ccctcagtgc cacatcccca cagttcttca tcacctccag ggacggtgac ccccccacct
     3781 ccgtgggcag ctgtgccact gcagcaccgc tctttggaga aggtaaatct tgctaaatcc
     3841 agcccgaccc tcccctggca caacgtaagg ccattatctc tcatccaact ccaggacgga
     3901 gtcagtgagg atggggctct agggatccac gcgtgctagc tcagaccgtt agacgccagg
     3961 acgggctgtc aggctggcgc cgcggcgcca gcctgacagc ccgtcctggc gtctaacggt
     4021 ctgagctagc ccgggctcga gatctgggct ataaaagggg gtgggggcgc gttcgtcctc
     4081 actctcttcc aagcttgcca caaccatggt tatgccagag ccagcgaagt ctgctcccgc
     4141 cccgaaaaag ggctccaaga aggcggtgac taaggcgcag aagaaaggcg gcaagaagcg
     4201 caagcgcagc cgcaaggaga gctattccat ctatgtgtac aaggttctga agcaggtcca
     4261 ccctgacacc ggcatttcgt ccaaggccat gggcatcatg aattcgtttg tgaacgacat
     4321 tttcgagcgc atcgcaggtg aggcttcccg cctggcgcat tacaacaagc gctcgaccat
     4381 cacctccagg gagatccaga cggccgtgcg cctgctgctg cctggggagt tggccaagca
     4441 cgccgtgtcc gagggtacta aggccatcac caagtacacc agcgctaagg atccaccggt
     4501 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     4561 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     4621 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     4681 gcccaccctc gtgaccaccc tgtcctgggg cgtgcagtgc ttcgcccgct accccgacca
     4741 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     4801 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     4861 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     4921 ggggcacaag ctggagtaca actacatcag cgacaacgtc tatatcaccg ccgacaagca
     4981 gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg gcggcgtgca
     5041 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     5101 caaccactac ctgagcaccc agtccaagct gagcaaagac cccaacgaga agcgcgatca
     5161 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     5221 caagtaatct agagtcgggg cggccggccg cttcgagcag acatgataag atacattgat
     5281 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     5341 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     5401 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
     5461 aacctctaca aatgtggtaa aatcgataag gatccgtcga ccgatgccct tgagagcctt
     5521 caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacggatccc
     5581 cgggtaccga gctcgctaga gggacagccc ccccccaaag cccccaggga tgtaattacg
     5641 tccctccccc gctagggggc agcagcgagc cgcccggggc tccgctccgg tccggcgctc
     5701 cccccgcatc cccgagccgg cagcgtgcgg ggacagcccg ggcacgggga aggtggcacg
     5761 ggatcgcttt cctctgaacg cttctcgctg ctctttgagc ctgcagacac ctggggggat
     5821 acggggaaaa agctttaggc tgaaagagag atttagaatg acagaatcat agaacggcct
     5881 gggttgcaaa ggagcacagt gctcatccag atccaacccc ctgctatgtg cagggtcatc
     5941 aaccagcagc ccaggctgcc cagagccaca tccagcctgg ccttgaatgc ctgcagggat
     6001 ggggcatcca cagcctcctt gggcaacctg ttcagtgcgt caccaccctc tgggggaaaa
     6061 actgcctcct catatccaac ccaaacctcc cctgtctcag tgtaaagcca ttcccccttg
     6121 tcctatcaag ggggagtttg ctgtgacatt gttggtctgg ggtgacacat gtttgccaat
     6181 tcagtgcatc acggagaggc agatcttggg gataaggaag tgcaggacag catggacgtg
     6241 ggacatgcag gtgttgaggg ctctgggaca ctctccaagt cacagcgttc agaacagcct
     6301 taaggataag aagataggat agaaggacaa agagcaagtt aaaacccagc atggagagga
     6361 gcacaaaaag gccacagaca ctgctggtcc ctgtgtctga gcctgcatgt ttgatggtgt
     6421 ctggatgcaa gcagaagggg tggaagagct tgcctggaga gatacagctg ggtcagtagg
     6481 actgggacag gcagctggag aattgccatg tagatgttca tacaatcgtc aaatcatgaa
     6541 ggctggaaaa gccctccaag atccccaaga ccaaccccaa cccacccacc gtgcccactg
     6601 gccatgtccc tcagtgccac atccccacag ttcttcatca cctccaggga cggtgacccc
     6661 cccacctccg tgggcagctg tgccactgca gcaccgctct ttggagaagg taaatcttgc
     6721 taaatccagc ccgaccctcc cctggcacaa cgtaaggcca ttatctctca tccaactcca
     6781 ggacggagtc agtgaggatg gggctctagc gagctcgaat tc
//
