LOCUS       sequence-355570-       10185 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human LTBR linked to puromycin resistance via P2A for 
            lentiviral delivery..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10185)
  AUTHORS   Legut M, Gajic Z, Guarino M, Daniloski Z, Rahman JA, Xue X, Lu C, Lu
            L, Mimitou EP, Hao S, Davoli T, Diefenbach C, Smibert P, Sanjana NE
  TITLE     A genome-scale screen for synthetic drivers of T cell proliferation.
  JOURNAL   Nature. 2022 Mar;603(7902):728-735. doi: 10.1038/s41586-022-04494-7.
            Epub 2022 Mar 16.
  PUBMED    35296855
REFERENCE   2  (bases 1 to 10185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-022-04494-7"; journalName: "Nature"; date: 
            "2022-03"; volume: "603"; issue: "7902"; pages: "728-735"
COMMENT     Sequence Label: pOT_1 - lenti-EFS-LTBR-2A-puro
FEATURES             Location/Qualifiers
     source          1..10185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(51..70)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        242..621
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        623..821
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     776..796
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             839..1019
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1066..1191
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1684..1917
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2102..2146
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2444..2561
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2655..2866
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             4203..4259
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4260..4856
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     4753..4773
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4881..5469
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4934..4954)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5352..5363)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5472..5488)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5473..5489)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5994..6174
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6200..6217)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6206..6430
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6476..6904
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6563..6582)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6773..6794
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6913..6933)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6918..7247
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7098..7233
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7160..7179
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7295..7342
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7361..7735
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7865..7986
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7902..7921)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7956..7975
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8035..8051)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8035..8051)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8048..8070)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8059..8075
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8083..8089,8090..8107,8108..8113))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8128..8149
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8266..8283)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8437..9025)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8517..8536)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9196..9987,9988..10056))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9819..9838
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10057..10161)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc
       61 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
      121 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
      181 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
      241 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
      301 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
      361 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
      421 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      481 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      541 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      601 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
      661 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
      721 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
      781 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg cctgtactgg
      841 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
      901 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
      961 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     1021 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     1081 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     1141 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     1201 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     1261 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     1321 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     1381 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     1441 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     1501 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
     1561 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     1621 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     1681 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     1741 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     1801 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     1861 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     1921 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     1981 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     2041 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     2101 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     2161 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     2221 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     2281 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     2341 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
     2401 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
     2461 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     2521 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     2581 gcagagatcc agtttggtta attaagctag ctaggtcttg aaaggagtgg gaattggctc
     2641 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
     2701 ggtcggcaat tgatccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
     2761 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
     2821 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggacc ggttctagag
     2881 ccgccaccat gctcctgcct tgggccacct ctgcccccgg cctggcctgg gggcctctgg
     2941 tgctgggcct cttcgggctc ctggcagcat cgcagcccca ggcggtgcct ccatatgcgt
     3001 cggagaacca gacctgcagg gaccaggaaa aggaatacta tgagccccag caccgcatct
     3061 gctgctcccg ctgcccgcca ggcacctatg tctcagctaa atgtagccgc atccgggaca
     3121 cagtttgtgc cacatgtgcc gagaattcct acaacgagca ctggaactac ctgaccatct
     3181 gccagctgtg ccgcccctgt gacccagtga tgggcctcga ggagattgcc ccctgcacaa
     3241 gcaaacggaa gacccagtgc cgctgccagc cgggaatgtt ctgtgctgcc tgggccctcg
     3301 agtgtacaca ctgcgagcta ctttctgact gcccgcctgg cactgaagcc gagctcaaag
     3361 atgaagttgg gaagggtaac aaccactgcg tcccctgcaa ggccgggcac ttccagaata
     3421 cctcctcccc cagcgcccgc tgccagcccc acaccaggtg tgagaaccaa ggtctggtgg
     3481 aggcagctcc aggcactgcc cagtccgaca caacctgcaa aaatccatta gagccactgc
     3541 ccccagagat gtcaggaacc atgctgatgc tggccgttct gctgccactg gccttctttc
     3601 tgctccttgc caccgtcttc tcctgcatct ggaagagcca cccttctctc tgcaggaaac
     3661 tgggatcgct gctcaagagg cgtccgcagg gagagggacc caatcctgta gctggaagct
     3721 gggagcctcc gaaggcccat ccatacttcc ctgacttggt acagccactg ctacccattt
     3781 ctggagatgt ttccccagta tccactgggc tccccgcagc cccagttttg gaggcagggg
     3841 tgccgcaaca gcagagtcct ctggacctga ccagggagcc gcagttggaa cccggggagc
     3901 agagccaggt ggcccacggt accaatggca ttcatgtcac cggcgggtct atgactatca
     3961 ctggcaacat ctacatctac aatggaccag tactgggggg accaccgggt cctggagacc
     4021 tcccagctac ccccgaacct ccatacccca ttcccgaaga gggggaccct ggccctcccg
     4081 ggctctctac accccaccag gaagatggca aggcttggca cctagcggag acagagcact
     4141 gtggtgccac accctctaac aggggcccaa ggaaccaatt tatcacccat gacggatccg
     4201 gcgcaacaaa cttctctctg ctgaaacaag ccggagatgt cgaagagaat cctggaccga
     4261 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     4321 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     4381 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     4441 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     4501 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     4561 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     4621 cgtggttcct ggccaccgtc ggagtctcgc ccgaccacca gggcaagggt ctgggcagcg
     4681 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     4741 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     4801 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgagaat
     4861 tcgatatcaa gcttatcggt aatcaacctc tggattacaa aatttgtgaa agattgactg
     4921 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     4981 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     5041 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     5101 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     5161 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     5221 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     5281 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     5341 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     5401 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     5461 cctccccgca tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta
     5521 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     5581 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     5641 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     5701 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga
     5761 actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag
     5821 taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac
     5881 accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg
     5941 acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc
     6001 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     6061 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     6121 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc
     6181 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     6241 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     6301 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     6361 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     6421 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     6481 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     6541 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     6601 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     6661 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     6721 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     6781 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     6841 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     6901 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     6961 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     7021 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     7081 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     7141 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     7201 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     7261 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta
     7321 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc
     7381 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
     7441 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
     7501 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
     7561 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
     7621 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
     7681 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt
     7741 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     7801 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     7861 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     7921 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     7981 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     8041 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     8101 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     8161 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     8221 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     8281 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     8341 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     8401 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     8461 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     8521 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     8581 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     8641 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     8701 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     8761 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     8821 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     8881 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     8941 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     9001 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     9061 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     9121 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     9181 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     9241 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     9301 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     9361 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     9421 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     9481 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     9541 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     9601 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     9661 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     9721 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     9781 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9841 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9901 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9961 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    10021 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    10081 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    10141 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacc
//
