LOCUS       sequence-355573-        9687 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human truncated NGFR linked to puromycin resistance via 
            P2A for lentiviral delivery..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9687)
  AUTHORS   Legut M, Gajic Z, Guarino M, Daniloski Z, Rahman JA, Xue X, Lu C, Lu
            L, Mimitou EP, Hao S, Davoli T, Diefenbach C, Smibert P, Sanjana NE
  TITLE     A genome-scale screen for synthetic drivers of T cell proliferation.
  JOURNAL   Nature. 2022 Mar;603(7902):728-735. doi: 10.1038/s41586-022-04494-7.
            Epub 2022 Mar 16.
  PUBMED    35296855
REFERENCE   2  (bases 1 to 9687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-022-04494-7"; journalName: "Nature"; date: 
            "2022-03"; volume: "603"; issue: "7902"; pages: "728-735"
COMMENT     Sequence Label: pOT_2 - lenti-EFS-tNGFR-2A-puro
FEATURES             Location/Qualifiers
     source          1..9687
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(93..112)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        284..663
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        665..863
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     818..838
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             881..1061
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1108..1233
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1726..1959
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2144..2188
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2486..2603
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2697..2908
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             3747..3803
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3804..4400
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     4297..4317
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4425..5013
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4478..4498)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4896..4907)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5016..5032)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5017..5033)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5538..5718
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5744..5761)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5750..5974
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6020..6448
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6107..6126)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6317..6338
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6457..6477)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6462..6791
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6642..6777
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6704..6723
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6839..6886
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6905..7279
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7409..7530
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7446..7465)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7500..7519
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7579..7595)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7579..7595)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7592..7614)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7603..7619
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7627..7633,7634..7651,7652..7657))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7672..7693
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7810..7827)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7981..8569)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8061..8080)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8740..9531,9532..9600))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9363..9382
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9601..9687,1..18))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
       61 agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
      121 agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt
      181 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg
      241 cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact
      301 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
      361 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      421 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      481 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      541 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      601 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      661 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      721 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      781 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      841 cggtgggagg tctatataag cagcgcgttt tgcctgtact gggtctctct ggttagacca
      901 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
      961 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     1021 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     1081 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     1141 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     1201 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     1261 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     1321 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     1381 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     1441 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     1501 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     1561 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     1621 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     1681 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     1741 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     1801 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     1861 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     1921 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     1981 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     2041 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     2101 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     2161 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     2221 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     2281 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     2341 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     2401 agacagagac agatccattc gattagtgaa cggatcggca ctgcgtgcgc caattctgca
     2461 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     2521 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     2581 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttggt
     2641 taattaagct agctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
     2701 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgatccgg
     2761 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     2821 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     2881 tcgcaacggg tttgccgcca gaacacagga ccggttctag agccgccacc atgtacgacg
     2941 ggccgcgcct gctgctgttg ctgcttctgg gggtgtccct tggaggtgcc aaggaggcat
     3001 gccccacagg cctgtacaca cacagcggtg agtgctgcaa agcctgcaac ctgggcgagg
     3061 gtgtggccca gccttgtgga gccaaccaga ccgtgtgtga gccctgcctg gacagcgtga
     3121 cgttctccga cgtggtgagc gcgaccgagc cgtgcaagcc gtgcaccgag tgcgtggggc
     3181 tccagagcat gtcggcgccg tgcgtggagg ccgacgacgc cgtgtgccgc tgcgcctacg
     3241 gctactacca ggatgagacc actgggcgct gcgaggcgtg ccgcgtgtgc gaggcgggct
     3301 cgggcctcgt gttctcctgc caggacaagc agaacaccgt gtgcgaggag tgccccgacg
     3361 gcacgtattc cgacgaggcc aaccacgtgg acccgtgcct gccctgcacc gtgtgcgagg
     3421 acaccgagcg ccagctccgc gagtgcacac gctgggccga cgccgagtgc gaggagatcc
     3481 ctggccgttg gattacacgg tccacacccc cagagggctc ggacagcaca gcccccagca
     3541 cccaggagcc tgaggcacct ccagaacaag acctcatagc cagcacggtg gcaggtgtgg
     3601 tgaccacagt gatgggcagc tcccagcccg tggtgacccg aggcaccacc gacaacctca
     3661 tccctgtcta ttgctccatc ctggctgctg tggttgtggg tcttgtggcc tacatagcct
     3721 tcaagaggtg gaacagtgga tccggcgcaa caaacttctc tctgctgaaa caagccggag
     3781 atgtcgaaga gaatcctgga ccgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     3841 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     3901 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     3961 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     4021 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     4081 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     4141 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggagtc tcgcccgacc
     4201 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     4261 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     4321 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     4381 cccgcaagcc cggtgcctga gaattcgata tcaagcttat cggtaatcaa cctctggatt
     4441 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     4501 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     4561 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     4621 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     4681 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     4741 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     4801 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     4861 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     4921 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     4981 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagacctag
     5041 aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc
     5101 tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac
     5161 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     5221 aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac
     5281 aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga
     5341 cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg
     5401 aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     5461 gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc
     5521 tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     5581 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     5641 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     5701 tgtggaaaat ctctagcagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     5761 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     5821 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     5881 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     5941 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     6001 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     6061 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     6121 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     6181 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     6241 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     6301 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     6361 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     6421 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     6481 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     6541 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     6601 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     6661 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     6721 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     6781 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt
     6841 tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta
     6901 aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc
     6961 ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc
     7021 cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc
     7081 ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc
     7141 ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga
     7201 gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt
     7261 ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga
     7321 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     7381 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     7441 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     7501 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     7561 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     7621 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     7681 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     7741 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     7801 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     7861 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     7921 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     7981 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     8041 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     8101 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     8161 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     8221 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     8281 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     8341 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     8401 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     8461 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     8521 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     8581 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     8641 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     8701 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     8761 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     8821 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     8881 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     8941 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     9001 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     9061 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     9121 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     9181 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     9241 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     9301 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     9361 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     9421 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     9481 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     9541 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     9601 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     9661 catatttgaa tgtatttaga aaaataa
//
