LOCUS       sequence-355588-        8584 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA
            synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8584)
  AUTHORS   Arsic A, Hagemann C, Stajkovic N, Schubert T, Nikic-Spiegel I
  TITLE     Minimal genetically encoded tags for fluorescent protein labeling in
            living neurons.
  JOURNAL   Nat Commun. 2022 Jan 14;13(1):314. doi: 10.1038/s41467-022-27956-y.
  PUBMED    35031604
REFERENCE   2  (bases 1 to 8584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-01-14"; volume: "13(1):314. doi"; pages: " 
            10.1038/s41467-022-27956-y"
COMMENT     Sequence Label: pcDNA3.1(+)_U6 tRNAPyl_CMV
            NESPylRS(AF)_IRES_e...
FEATURES             Location/Qualifiers
     source          1..8584
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     635..654
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        635..653
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      673..682
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             703..729
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     misc_feature    2119..2670
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2263..2280)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2490..2509
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      2695..2704
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2725..2751
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             2755..2796
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4105..4131
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(4182..4199)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4188..4412
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4458..4886
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4545..4564)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4755..4776
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4895..4915)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4900..5230
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5081..5216
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5143..5162
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5278..5325
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5344..5718
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5848..5969
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5885..5904)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5939..5958
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6018..6034)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6018..6034)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6031..6053)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6042..6058
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6066..6072,6073..6090,6091..6096))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6111..6132
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6249..6266)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6420..7008)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6500..6519)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7179..7970,7971..8039))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7802..7821
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8040..8144)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        complement(8268..8516)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(8326..8345)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(8496..8516)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa
      601 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
      661 aagctggcta gcgccaccat ggcttgccct gtgcctctgc agctgcctcc cctggaacgc
      721 ctgaccctgg acgataagaa accactgaac accctgatta gcgccaccgg cctgtggatg
      781 tccaggaccg gcacaatcca caagatcaag caccacgagg tgagcagatc caaaatctac
      841 atcgagatgg cctgcggcga ccacctggtg gtgaacaaca gcaggagcag caggaccgcc
      901 cgggccctga ggcaccacaa gtatcgcaag acatgcaagc ggtgtcgcgt gtccgacgag
      961 gatctgaaca agttcctgac caaggccaat gaggatcaga catccgtgaa ggtgaaggtg
     1021 gtgtctgccc caacccggac aaagaaggca atgccaaaga gcgtggccag ggcccccaag
     1081 cctctggaga acaccgaggc agcacaggca cagccatctg gcagcaagtt tagcccagca
     1141 atccccgtga gcacccagga gtccgtgtct gtgcctgcca gcgtgtccac atctatctct
     1201 agcatctcta ccggagcaac agccagcgcc ctggtgaagg gcaacaccaa tcctatcaca
     1261 agcatgtccg ccccagtgca ggccagcgcc cctgccctga ccaagtccca gacagaccgc
     1321 ctggaggtgc tgctgaaccc taaggatgag atcagcctga acagcggcaa gccattcagg
     1381 gagctggagt ccgagctgct gtctaggaga aagaaggacc tgcagcaaat ctacgccgag
     1441 gagagggaga actatctggg caagctggag agggagatca ccagattctt tgtggataga
     1501 ggctttctgg agatcaagtc cccaatcctg atccccctgg agtacatcga gcggatgggc
     1561 atcgacaacg atacagagct gtctaagcag attttcaggg tggacaagaa tttttgcctg
     1621 aggcccatgc tggccccaaa cctggccaat tacctgagga agctggaccg cgccctgccc
     1681 gaccccatca agattttcga gatcggccca tgttatagaa aggagtctga tggcaaggag
     1741 cacctggagg agtttaccat gctgaatttt tgccagatgg gctctggctg tacccgggag
     1801 aacctggaga gcatcatcac agacttcctg aatcacctgg gcatcgactt taagatcgtg
     1861 ggcgatagct gtatggtgtt tggcgacaca ctggatgtga tgcacggcga tctggagctg
     1921 tcctctgccg tggtgggacc tatcccactg gacagggagt ggggcatcga taagccctgg
     1981 atcggagcag gattcggcct ggagcgcctg ctgaaggtga agcacgactt taagaacatc
     2041 aagcgggctg ccagaagcga aagttattac aatgggatta gcacaaacct gtgaaagctt
     2101 ggtaccgagc tcggatccac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     2161 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     2221 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     2281 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     2341 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     2401 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     2461 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     2521 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
     2581 gctttacatg tgtttagtcg aggttaaaaa acgtctaggc cccccgaacc acggggacgt
     2641 ggttttcctt tgaaaaacac gatgataata gaattcagac ccaagctggc tagcgccacc
     2701 atggcgtgcc cggtgccgct gcagctgccg ccgctggaac gcctgaccct ggatggcaag
     2761 cctattccca accccctgct gggcctggat agcaccgctg acgacccctc cgctgccgat
     2821 agaaacgtgg aaatctggaa gatcaagaaa ctgattaaga gcctggaggc tgccagagga
     2881 aacggcacca gcatgatctc cctgattatc cctcccaagg atcagatcag cagggtcgct
     2941 aagatgctcg ccgatgattt cggcaccgcc agcaatatca agagcagggt gaacagactg
     3001 agcgtgctgg gcgccattac cagcgtgcag cagagactga agctgtacaa caaggtgccc
     3061 cccaacggac tggtggtgta ctgtggaacc atcgtgaccg aagagggcaa ggaaaaaaaa
     3121 gtgaacatcg acttcgagcc cttcaaaccc atcaacacct ccctctacct gtgcgacaat
     3181 aagttccaca ccgaggccct gacagctctg ctcagcgacg actccaagtt cggcttcatt
     3241 gtgatcgacg gaagcggcgc tctgttcgga accctgcagg gcaacaccag ggaggtgctc
     3301 cacaagttca ccgtggacct gcctaaaaag cacggcagag gcggccagag cgctctgaga
     3361 ttcgccaggc tgagaatgga gaagaggcac aactacgtga gaaaggtggc cgaaaccgcc
     3421 gtgcagctgt tcattagcgg cgacaaggtg aacgtggccg gcctggtgct ggccggctcc
     3481 gccgacttta agaccgagct gtcccagagc gatatgttcg atcagaggct gcaaagcaag
     3541 gtgctgaagc tggtggacat cagctacgga ggcgagaacg gcttcaacca ggccatcgaa
     3601 ctcagcaccg aggtgctgag caacgtgaaa ttcattcaag agaagaagct catcggcagg
     3661 tacttcgacg agatcagcca ggataccggc aagtactgct ttggagtgga ggataccctg
     3721 aaggccctgg agatgggcgc cgtggagatc ctgattgtgt acgagaatct ggacatcatg
     3781 agatatgtgc tccactgcca gggcaccgag gaggagaaaa tcctgtacct cacccccgag
     3841 caggagaagg acaaaagcca cttcaccgat aaggagaccg gccaggaaca cgagctgatt
     3901 gagtccatgc ccctgctgga gtggtttgcc aacaactaca agaaattcgg cgccaccctc
     3961 gagatcgtca ccgacaagtc ccaggaaggc tcccagttcg tcaagggctt cggcggcatc
     4021 ggaggcatcc tcaggtacag ggtcgacttc cagggcatgg agtatcaggg cggcgacgac
     4081 gagttcttcg atctggacga ctactaccca tacgatgttc cagattacgc ttaatgagcg
     4141 gccgctcgag tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
     4201 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     4261 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     4321 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     4381 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     4441 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     4501 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     4561 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc ggggcatccc
     4621 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     4681 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     4741 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     4801 ctattctttt gatttataag ggattttggg gatttcggcc tattggttaa aaaatgagct
     4861 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
     4921 aagtccccag gctccccagg caggcagaag tatgcaaagc atgcatctca attagtcagc
     4981 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     5041 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     5101 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     5161 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     5221 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt
     5281 gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa
     5341 accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg
     5401 gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc
     5461 ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg
     5521 gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg
     5581 gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag
     5641 cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg
     5701 gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa
     5761 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     5821 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     5881 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     5941 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     6001 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     6061 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     6121 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     6181 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     6241 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     6301 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     6361 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     6421 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     6481 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     6541 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     6601 gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     6661 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     6721 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     6781 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     6841 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     6901 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     6961 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     7021 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     7081 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     7141 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     7201 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     7261 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     7321 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     7381 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     7441 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     7501 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     7561 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     7621 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     7681 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     7741 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     7801 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     7861 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     7921 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     7981 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     8041 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     8101 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     8161 gtgccacctg acgtcgacgg atcgggagat ctaaaaaacg gaaaccccgg gaatctaacc
     8221 cggctgaacg gatttagagt ccattcgatc tacatgatca ggtttccggt gtttcgtcct
     8281 ttccacaaga tatataaagc caagaaatcg aaatactttc aagttacggt aagcatatga
     8341 tagtccattt taaaacataa ttttaaaact gcaaactacc caagaaatta ttactttcta
     8401 cgtcacgtat tttgtactaa tatctttgtg tttacagtca aattaattcc aattatctct
     8461 ctaacagcct tgtatcgtat atgcaaatat gaaggaatca tgggaaatag gccctccaat
     8521 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
     8581 tata
//
