LOCUS       sequence-35559-e         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller 
            JA, Somia NV, Bogdanove AJ, Voytas DF
  TITLE     Efficient design and assembly of custom TALEN and other TAL 
            effector-based constructs for DNA targeting.
  JOURNAL   Nucleic Acids Res. 2011 Jul;39(12):e82. doi: 10.1093/nar/gkr218
  PUBMED    21493687
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-07"; volume: "39(12):e82. doi"; pages: " 
            10.1093/nar/gkr21"
COMMENT     Sequence Label: pFUS_B8
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..378)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGAGLDSH
                     HLVRGQAMRRLRLAVVLQRRDW"
     primer_bind     15..31
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     243..259
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     269..288
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        269..287
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(335..355)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(335..353)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(374..390)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(374..390)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(387..409)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    398..414
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(422..428,429..446,447..452))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    467..488
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ccagtcacga cgttgtaaaa cgacggccag tcttaagcgt ctcatggcct gaccccggac
       61 caagtggtgg ctatcgagac cggcgccgct acagggcgcg tcccattcgc cattcaggct
      121 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      181 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      241 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac
      301 cgggcccccc ctcgaggtcc tccagctttt gttcccttta gtgagggtta attgcgcgct
      361 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
      421 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
      481 tcacattaat tgcgttgcgc tcactgcccg ctttccaccg gtggtctcct cgaaacggtg
      541 cagcggctgt tgccggtgct gtgccaggac catggcctga ccccggacca agtggtggct
      601 atcgagacgt ctagaccagc caggacagaa atgcctcgac ttcgctgcta cccaaggttg
      661 ccgggtgacg cacaccgtgg aaacggatga aggcacgaac ccagtggaca taagcctgtt
      721 cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga
      781 ccgaacgcag cggtggtaac ggcgc
//
