LOCUS       sequence-355614-       13487 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual fluorescent reporter construct with 5kb lambda spacer, upstream
            TRE (Tetracycline Response Element) recruitment site, phiC31 attB 
            for integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13487)
  AUTHORS   Lensch S, Herschl MH, Ludwig CH, Sinha J, Hinks MM, Mukund A, 
            Fujimori T, Bintu L
  TITLE     Dynamic spreading of chromatin-mediated gene silencing and 
            reactivation between neighboring genes in single cells.
  JOURNAL   Elife. 2022 Jun 9;11. pii: 75115. doi: 10.7554/eLife.75115.
  PUBMED    35678392
REFERENCE   2  (bases 1 to 13487)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-06-9"; volume: "11. pii: 75115. doi"; pages: " 
            10.7554/eLife.75115"
COMMENT     Sequence Label: 
            pSL008_phiC31_neo-5xTetO-pEF-H2B-mCitrine-5kb,lambda-pRSV-H2B-mCherr
            y
FEATURES             Location/Qualifiers
     source          1..13487
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    16..85
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             351..1154
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(414..433)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1024..1043
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(1186..1203)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1192..1416
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    1489..1507
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1525..1543
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1561..1579
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1596..1614
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1632..1650
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     intron          1895..2833
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2790..2810
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        2866..2884
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             2975..3352
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      3371..3380
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3377..3379,3380..3382,3383..4093)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3422..3443)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3683..3702)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4030..4051
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4144..4163)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4145..4163)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    4437..4558
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    5164..5395
                     /gene="attP"
                     /label=lambda attP
                     /bound_moiety="lambda integrase"
                     /note="integrase from phage lambda"
     CDS             complement(5390..6460)
                     /codon_start=1
                     /gene="int"
                     /product="integrase from phage lambda"
                     /label=lambda integrase
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIAI
                     TEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMSKI
                     KAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTN
                     HVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCEMKWS
                     DIVDGYLYVEQSKTGVKIAIPTALHIDALGISMKETLDKCKEILGGETIIASTRREPLS
                     SGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMAS
                     QYRDDRGREWDKTEIK"
     terminator      complement(8681..8925)
                     /label=lambda tL3 terminator
                     /note="transcription terminator tL3 from phage lambda"
     promoter        9616..9878
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     CDS             9892..10269
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      10282..10291
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10288..11001
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYKS"
     primer_bind     complement(10433..10451)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     10701..10720
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    11117..11198
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        11241..11304
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11305..11373,11374..12165)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11523..11542)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12336..12924
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12825..12844
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    13108..13242
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    13345..13466
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 gatgtaggtc acggtctcga agccgcggtg cgggtgccag ggcgtgccct tgggctcccc
       61 gggcgcgtac tccacctcac ccatctggtc catcatgatg aacgggtcga ggtggcggta
      121 gttgatcccg gcgaacgcgc ggcgcaccgg gaagccctcg ccctcgaaac cgctgggcgc
      181 ggtggtcacg gtgagcacgg gacgtgcgac ggcgtcggcg ggtgcggata cgcggggcag
      241 cgtcagcggg ttctcgacgg tcacggcggg catgtcgacg gtatcgataa gcttacgcgt
      301 gctagcgcat gcagatctac tagtcggccg gataaagata tcaactcgag atgggatcgg
      361 ccattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
      421 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
      481 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
      541 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
      601 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
      661 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
      721 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
      781 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
      841 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
      901 gcgatgatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
      961 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     1021 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     1081 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     1141 acgagttctt ctgaggggat caattctcta gagctcgctg atcagcctcg actgtgcctt
     1201 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     1261 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     1321 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     1381 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     1441 gggggctagc aaagcctagg aagacgtcga tatacgcgtc gagtttactc cctatcagtg
     1501 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac
     1561 tccctatcag tgatagagaa cgtatgtcga gtttatccct atcagtgata gagaacgtat
     1621 gtcgagttta ctccctatca gtgatagaga acgtatgtcg aggtaggcgt gtacggtggg
     1681 aatgcatgag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     1741 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     1801 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     1861 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     1921 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
     1981 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     2041 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     2101 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     2161 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     2221 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     2281 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     2341 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     2401 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     2461 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     2521 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     2581 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     2641 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     2701 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     2761 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     2821 tcttccattt caggtgtcgt gagctagcga ttacgccaag ctcgaaatta accctcacta
     2881 aagggaacaa aagctggagc tccaccgcgg tggcggccgc tctagcccgg gcggatcccc
     2941 cgggctgcag gaattcgata tcaagcttgc caccatgcca gagccagcga agtctgctcc
     3001 cgccccgaaa aagggctcca agaaggcggt gactaaggcg cagaagaaag gcggcaagaa
     3061 gcgcaagcgc agccgcaagg agagctattc catctatgtg tacaaggttc tgaagcaggt
     3121 ccaccctgac accggcattt cgtccaaggc catgggcatc atgaattcgt ttgtgaacga
     3181 cattttcgag cgcatcgcag gtgaggcttc ccgcctggcg cattacaaca agcgctcgac
     3241 catcacctcc agggagatcc agacggccgt gcgcctgctg ctgcctgggg agttggccaa
     3301 gcacgccgtg tccgagggta ctaaggccat caccaagtac accagcgcta aggatccccg
     3361 ggtaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     3421 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     3481 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     3541 cgtgccctgg cccaccctcg tgaccacctt cggctacggc ctgatgtgct tcgcccgcta
     3601 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     3661 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     3721 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     3781 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     3841 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     3901 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     3961 gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc ccaacgagaa
     4021 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     4081 cgagctgtac aagtaggtcg acctcgaggg ggggcccggt accaggtaag tgtacccaat
     4141 tcgccctata gtgagtcgta ttacaattca ctcgatcgcc cttcccaaca gttgcgcagc
     4201 ctgaatggcg aatggagatc caatttttaa gtgtataatg tgttaaacta ctgattctaa
     4261 ttgtttgtgt attttagatt cacagtccca aggctcattt caggcccctc agtcctcaca
     4321 gtctgttcat gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     4381 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     4441 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     4501 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaac
     4561 gcgtaaattg taagcgtctc agcaatttat ttggcggcaa cacaggatct ctcttttaag
     4621 ttactctcta ttacatacgt tttccatcta aaaattagta gtattgaact taacggggca
     4681 tcgtattgta gttttccata tttagctttc tgcttccttt tggataaccc actgttattc
     4741 atgttgcatg gtgcactgtt tataccaacg atatagtcta ttaatgcata tatagtatcg
     4801 ccgaacgatt agctcttcag gcttctgaag aagcgtttca agtactaata agccgataga
     4861 tagccacgga cttcgtagcc atttttcata agtgttaact tccgctcatc gctcataaca
     4921 gacattcact acagttatgg cggaaaggta tgcatgctgg gtgtggggaa gtcgtgaaag
     4981 aaaagaagtc agctgcgtcg tttgacatca ctgctatctt cttactggtt atgcaggtcg
     5041 tagtgggtgg cacacaaagc tttgcactgg attgcgaggc tttgtgcttc tctggagtgc
     5101 gacaggtttg atgacaaaaa attagcgcaa gaagacaaaa atcaccttgc gctaatgctc
     5161 tgttacaggt cactaatacc atctaagtag ttgattcata gtgactgcat atgttgtgtt
     5221 ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata
     5281 tcattttacg tttctcgttc agctttttta tactaagttg gcattataaa aaagcattgc
     5341 ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgattt
     5401 cagttttgtc ccactccctg cctctgtcat cacgatactg tgatgccatg gtgtccgact
     5461 tatgcccgag aagatgttga gcaaacttat cgcttatctg cttctcatag agtcttgcag
     5521 acaaactgcg caactcgtga aaggtaggcg gatccccttc gaaggaaaga cctgatgctt
     5581 ttcgtgcgcg cataaaatac cttgatactg tgccggatga aagcggttcg cgacgagtag
     5641 atgcaattat ggtttctccg ccaagaatct ctttgcattt atcaagtgtt tccttcattg
     5701 atattccgag agcatcaata tgcaatgctg ttgggatggc aatttttacg cctgttttgc
     5761 tttgctcgac ataaagatat ccatctacga tatcagacca cttcatttcg cataaatcac
     5821 caactcgttg cccggtaaca acagccagtt ccattgcaag tctgagccaa catggtgatg
     5881 attctgctgc ttgataaatt ttcaggtatt cgtcagccgt aagtcttgat ctccttacct
     5941 ctgattttgc tgcgcgagtg gcagcgacat ggtttgttgt tatatggcct tcagctattg
     6001 cctctcggaa tgcatcgctc agtgttgatc tgattaactt ggctgacgcc gccttgccct
     6061 cgtctatgta tccattgagc attgccgcaa tttcttttgt ggtgatgtct tcaagtggag
     6121 catcaggcag acccctcctt attgctttaa ttttgctcat gtaatttatg agtgtcttct
     6181 gcttgattcc tctgctggcc aggatttttt cgtagcgatc aagccatgaa tgtaacgtaa
     6241 cggaattatc actgttgatt ctcgctgtca gaggcttgtg tttgtgtcct gaaaataact
     6301 caatgttggc ctgtatagct tcagtgattg cgattcgcct gtctctgcct aatccaaact
     6361 ctttacccgt ccttgggtcc ctgtagcagt aatatccatt gtttcttata taaaggttag
     6421 ggggtaaatc ccggcgctca tgacttcgcc ttcttcccat ttctgatcct cttcaaaagg
     6481 ccacctgtta ctggtcgatt taagtcaacc tttaccgctg attcgtggaa cagatactct
     6541 cttccatcct taaccggagg tgggaatatc ctgcattccc gaacccatcg acgaactgtt
     6601 tcaaggcttc ttggacgtcg ctggcgtgcg ttccactcct gaagtgtcaa gtacatcgca
     6661 aagtctccgc aattacacgc aagaaaaaac cgccatcagg cggcttggtg ttctttcagt
     6721 tcttcaattc gaatattggt tacgtctgca tgtgctatct gcgcccatat catccagtgg
     6781 tcgtagcagt cgttgatgtt ctccgcttcg ataactctgt tgaatggctc tccattccat
     6841 tctcctgtga ctcggaagtg catttatcat ctccataaaa caaaacccgc cgtagcgagt
     6901 tcagataaaa taaatccccg cgagagcgag gattgttatg taatattggg tttaatcatc
     6961 tatatgtttt gtacagagag ggcaagtatc gtttccaccg tactcgtgat aataattttg
     7021 cacggtatca gtcatttctc gcacattgca gaatggggat ttgtcttcat tagacttata
     7081 aaccttcatg gaatatttgt atgccgactc tatatctata ccttcatcta cataaacacc
     7141 ttcgtgatgt ctgcatggag acaagacacc ggatctgcac aacattgata acgcccaatc
     7201 tttttgctca gactctaact cattgatact catttataaa ctccttgcaa tgtatgtcgt
     7261 ttcagctaaa cggtatcagc aatgtttatg taaagaaaca gtaagataat actcaacccg
     7321 atgtttgagt acggtcatca tctgacacta cagactctgg catcgctgtg aagacgacgc
     7381 gaaattcagc attttcacaa gcgttatctt ttacaaaacc gatctcactc tcctttgatg
     7441 cgaatgccag cgtcagacat catatgcaga tactcacctg catcctgaac ccattgacct
     7501 ccaaccccgt aatagcgatg cgtaatgatg tcgatagtta ctaacgggtc ttgttcgatt
     7561 aactgccgca gaaactcttc caggtcacca gtgcagcgct tgataacagg agtcttccca
     7621 ggatggcgaa caacaagaaa ctggtttccg tcttcacgga cttcgttgct ttccagttta
     7681 gcaatacgct tactcccatc cgagataaca ccttcgtaat actcacgctg ctcgttgagt
     7741 tttgattttg ctgtttcaag ctcaacacgc agtttcccta ctgttagcgc aatatcctcg
     7801 ttctcctggt cgcggcgttt gatgtattgc tggtttcttt cccgttcatc cagcagttcc
     7861 agcacaatcg atggtgttac caattcatgg aaaaggtctg cgtcaaatcc ccagtcgtca
     7921 tgcattgcct gctctgccgc ttcacgcagt gcctgagagt taatttcgct cacttcgaac
     7981 ctctctgttt actgataagt tccagatcct cctggcaact tgcacaagtc cgacaaccct
     8041 gaacgaccag gcgtcttcgt tcatctatcg gatcgccaca ctcacaacaa tgagtggcag
     8101 atatagcctg gtggttcagg cggcgcattt ttattgctgt gttgcgctgt aattcttcta
     8161 tttctgatgc tgaatcaatg atgtctgcca tctttcatta atccctgaac tgttggttaa
     8221 tacgcttgag ggtgaatgcg aataataaaa aaggagcctg tagctccctg atgattttgc
     8281 ttttcatgtt catcgttcct taaagacgcc gtttaacatg ccgattgcca ggcttaaatg
     8341 agtcggtgtg aatcccatca gcgttaccgt ttcgcggtgc ttcttcagta cgctacggca
     8401 aatgtcatcg acgtttttat ccggaaactg ctgtctggct ttttttgatt tcagaattag
     8461 cctgacgggc aatgctgcga agggcgtttt cctgctgagg tgtcattgaa caagtcccat
     8521 gtcggcaagc ataagcacac agaatatgaa gcccgctgcc agaaaaatgc attccgtggt
     8581 tgtcatacct ggtttctctc atctgcttct gctttcgcca ccatcatttc cagcttttgt
     8641 gaaagggatg cggctaacgt atgaaattct tcgtctgttt ctactggtat tggcacaaac
     8701 ctgattccaa tttgagcaag gctatgtgcc atctcgatac tcgttcttaa ctcaacagaa
     8761 gatgctttgt gcatacagcc cctcgtttat tatttatctc ctcagccagc cgctgtgctt
     8821 tcagtggatt tcggataaca gaaaggccgg gaaataccca gcctcgcttt gtaacggagt
     8881 agacgaaagt gattgcgcct acccggatat tatcgtgagg atgcgtcatc gccattgctc
     8941 cccaaataca aaaccaattt cagccagtgc ctcgtccatt ttttcgatga actccggcac
     9001 gatctcgtca aaactcgcca tgtacttttc atcccgctca atcacgacat aatgcaggcc
     9061 ttcacgcttc atacgcgggt catagttggc aaagtaccag gcattttttc gcgtcaccca
     9121 catgctgtac tgcacctggg ccatgtaagc tgactttatg gcctcgaaac caccgagccg
     9181 gaacttcatg aaatcccggg aggtaaacgg gcatttcagt tcaaggccgt tgccgtcact
     9241 gcataaacca tcgggagagc aggcggtacg catactttcg tcgcgataga tgatcgggga
     9301 ttcagtaaca ttcacgccgg aagtgaattc aaacagggtt ctggcgtcgt tctcgtactg
     9361 ttttccccag gccagtgctt tagcgttaac ttccggagcc acaccggtgc aaacctcagc
     9421 aagcagggtg tggaagtagg acattttcat gtcaggccac ttctttccgg agcggggttt
     9481 tgctatcacg ttgtgaactt ctgaagcggt gatgacgccg agccgtaatt tgtgccacgc
     9541 atcatccccc tgttcgacag ctctcacatc gatcccggta cgctgcagga taatgtccct
     9601 agggacgtcc atatgaatgt agtcttatgc aatacacttg tagtcttgca acatggtaac
     9661 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
     9721 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acaggtctga catggattgg
     9781 acgaaccact gaattgcgca ttgcagagat aattgtattt aagtgcctag ctcgatacaa
     9841 taaacgccat ttgaccattc accacattgg tgtgcaccct gcagatctag aatgccagag
     9901 ccagcgaagt ctgctcccgc cccgaaaaag ggctccaaga aggcggtgac taaggcgcag
     9961 aagaaaggcg gcaagaagcg caagcgcagc cgcaaggaga gctattccat ctatgtgtac
    10021 aaggttctga agcaggtcca ccctgacacc ggcatttcgt ccaaggccat gggcatcatg
    10081 aattcgtttg tgaacgacat tttcgagcgc atcgcaggtg aggcttcccg cctggcgcat
    10141 tacaacaagc gctcgaccat cacctccagg gagatccaga cggccgtgcg cctgctgctg
    10201 cctggggagt tggccaagca cgccgtgtcc gagggtacta aggccatcac caagtacacc
    10261 agcgctaagg atccaccggt cgccaccatg gtgagcaagg gcgaggagga taacatggcc
    10321 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
    10381 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
    10441 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
    10501 tacggctcca aggcctacgt gaagcacccc gccgacattc ccgactactt gaagctgtcc
    10561 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
    10621 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
    10681 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
    10741 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
    10801 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
    10861 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
    10921 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
    10981 gacgagctgt acaagtctta agcgactcta gatcataatc agccatacca catttgtaga
    11041 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
    11101 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
    11161 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
    11221 actcatcaat gtatctcata tgtgtatccg ctcatgagac aataaccctg ataaatgctt
    11281 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
    11341 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
    11401 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
    11461 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
    11521 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
    11581 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
    11641 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
    11701 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
    11761 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
    11821 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
    11881 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
    11941 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
    12001 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
    12061 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
    12121 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
    12181 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
    12241 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
    12301 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
    12361 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
    12421 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
    12481 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
    12541 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
    12601 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
    12661 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
    12721 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
    12781 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
    12841 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
    12901 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
    12961 ttttgctggc cttttgctca catgttcttt cctgcggtac cgatcataat cagccatacc
    13021 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
    13081 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
    13141 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
    13201 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcataatcag ccataccaca
    13261 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
    13321 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
    13381 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
    13441 ttgtccaaac tcatcaatgt atcttatcat gtctgctcga ggtcgac
//
