LOCUS       sequence-355623-        4335 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC22A14_STOP
FEATURES             Location/Qualifiers
     source          1..4335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1892..1991)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2008..2027)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2009..2027)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2032..2048)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2032..2048)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2150..2169)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2161..2970
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2228..2247)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3117..3705
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3606..3625
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3859..3876
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4035..4062)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4154..4240)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4289..4311
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4303..4320
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4304..4320
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggc cggcgaggag
      121 aacttcaagg aggagctgcg gagccaggac gcatccagaa acctgaatca gcacgaggtg
      181 gccggccacc ctcactcttg gagcctggag atgctgctgc ggagactgag ggcagtgcac
      241 accaagcagg acgataagtt tgccaacctg ctggacgcag tgggagagtt cggcaccttt
      301 cagcagcggc tggtggccct gacattcatc ccatctatca tgagcgcctt ctttatgttt
      361 gccgatcact tcgtgtttac cgcccagaag ccttactgca atacatcctg gatcctggcc
      421 gtgggcccac acctgtctaa ggccgagcag ctgaacctga ccatcccaca ggcccccaat
      481 ggctctttcc tgacatgttt tatgtacctg cccgtgcctt ggaacctgga cagcatcatc
      541 cagttcggcc tgaatgacac cgatacatgc caggacggct ggatctatcc cgatgccaag
      601 aagagatctc tgatcaacga gtttgacctg gtgtgcggca tggagaccaa gaaggataca
      661 gcccagatca tgttcatggc cggcctgcca atcggcagcc tgatctttag gctgatcacc
      721 gataagatgg gccgctatcc agcaatcctg ctgtccctgc tgggactgat catcttcggc
      781 tttggcacag ccttcatgaa ttcttttcac ctgtacctgt tctttagatt cggcatctcc
      841 cagtctgtgg tgggctatgc catcagctcc atctccctgg caaccgagtg gctggtggga
      901 gagcacaggg cacacgccat catcctgggc cactgcttct ttgcagtggg agccgtgctg
      961 ctgacaggaa tcgcctacag cctgccccac tggcagctgc tgttcctggt gggcggcatc
     1021 ctggtcatcc cttttatctc ctatatctgg atcctgcctg agtctccacg ctggctgatg
     1081 atgaagggca aggtgaagga ggccaagcag gtgctgtgct acgccgccag cgtgaacaag
     1141 aagaccatcc catccaatct gctggacgag ctgcagctgc cccggaagaa ggtgacaaga
     1201 gcctccgtgc tggatttctg caagaatcgg cagctgtgca aggtgaccct ggtcatgtcc
     1261 tgcgtgtggt tcacagtgtc ttacacctac ttcaccctga gcctgaggat gagggagctg
     1321 ggcgtgtctg tgcacttccg gcacgtggtg cctagcatca tggaggtgcc agccagactg
     1381 tgctgtatct ttctgctgca gcagatcggc aggaagtgga gcctggcagt gaccctgctg
     1441 caggccatca tctggtgcct gctgctgctg ttcctgccag agggagagga tggactgagg
     1501 ctgaagtggc caaggtgtcc tgcaaccgag ctgaagagca tgacaatcct ggtgctgatg
     1561 ctgagagagt tttccctggc cgccaccgtg acagtgttct ttctgtatac cgcagagctg
     1621 ctgcctaccg tgctgagggc aacaggactg ggactggtgt ccctggcatc tgtggcaggc
     1681 gccatcctga gcctgaccat catctctcag acaccaagcc tgctgcccat cttcctgtgc
     1741 tgcgtgctgg ccatcgtggc cttttccctg tctagcctgc tgccagagac cagggaccag
     1801 cctctgagcg agtccctgaa ccactcctct cagatccgga ataaggtgaa ggatatgaag
     1861 accaaggaga caagctccga cgatgtgtaa gacccagctt tcttgtacaa agttggcatt
     1921 ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
     1981 atcattattt gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct
     2041 gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa
     2101 aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt
     2161 atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat
     2221 ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc
     2281 ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc
     2341 aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg
     2401 accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc
     2461 ggaaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat
     2521 gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac
     2581 agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat
     2641 gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg
     2701 cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat
     2761 aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc
     2821 gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca
     2881 ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag
     2941 tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg
     3001 cagagcatta cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg
     3061 agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     3121 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     3181 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     3241 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     3301 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     3361 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     3421 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     3481 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     3541 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     3601 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     3661 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     3721 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     3781 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     3841 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     3901 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     3961 gactggaaag cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag
     4021 tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg
     4081 cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat
     4141 ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
     4201 gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact
     4261 ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac gttgtaaaac gacggccagt
     4321 cttaagctcg ggccc
//
