LOCUS       sequence-355624-        4080 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC2A4_STOP
FEATURES             Location/Qualifiers
     source          1..4080
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1637..1736)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1753..1772)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1754..1772)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1777..1793)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1777..1793)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1895..1914)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1906..2715
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1973..1992)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2862..3450
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3351..3370
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3604..3621
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3780..3807)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3899..3985)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4034..4056
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4048..4065
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4049..4065
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgcc ttctggcttc
      121 cagcagatcg gcagcgagga cggagagcca cctcagcaga gggtgaccgg cacactggtg
      181 ctggccgtgt tctctgccgt gctgggcagc ctgcagtttg gctacaacat cggcgtgatc
      241 aatgccccac agaaagtgat cgagcagagc tataacgaga cctggctggg ccggcaggga
      301 ccagagggac ctagctccat cccacccggc acactgacca cactgtgggc cctgtccgtg
      361 gccatcttct ctgtgggcgg catgatctct agctttctga tcggcatcat ctcccagtgg
      421 ctgggaagga agagggcaat gctggtgaac aatgtgctgg ccgtgctggg aggatctctg
      481 atgggactgg caaacgcagc agcaagctac gagatgctga tcctgggcag gttcctgatc
      541 ggagcatact ctggactgac cagcggactg gtgccaatgt atgtgggcga gatcgcacca
      601 acccacctga ggggcgccct gggcacactg aatcagctgg ccatcgtgat cggcatcctg
      661 atcgcacagg tgctgggact ggagagcctg ctgggaaccg catccctgtg gcctctgctg
      721 ctgggcctga cagtgctgcc agccctgctg cagctggtgc tgctgccatt ttgccccgag
      781 agccctaggt acctgtatat catccagaat ctggagggac cagcaaggaa gtccctgaag
      841 cgcctgaccg gatgggcaga cgtgtctggc gtgctggccg agctgaagga tgagaagcgc
      901 aagctggagc gggagagacc cctgtccctg ctgcagctgc tgggctctcg gacacacaga
      961 cagcctctga tcatcgccgt ggtgctgcag ctgtcccagc agctgtctgg catcaacgcc
     1021 gtgttctact attccacctc tatctttgag acagcaggag tgggacagcc agcatacgca
     1081 accatcggcg ccggcgtggt gaataccgtg ttcacactgg tgtctgtgct gctggtggag
     1141 agggcaggcc ggagaaccct gcacctgctg ggactggcag gaatgtgcgg ctgtgccatc
     1201 ctgatgacag tggccctgct gctgctggag agagtgcctg ccatgagcta cgtgagcatc
     1261 gtggccatct tcggctttgt ggccttcttt gagatcggcc ctggcccaat cccctggttc
     1321 atcgtggccg agctgttttc ccagggacct agaccagcag caatggcagt ggcaggcttt
     1381 agcaactgga cctccaattt catcatcggc atgggctttc agtacgtggc cgaggccatg
     1441 ggcccatacg tgttcctgct gtttgccgtg ctgctgctgg gcttctttat cttcacattt
     1501 ctgagggtgc cagagaccag gggacgcaca ttcgatcaga tcagcgccgc ctttcacaga
     1561 accccctccc tgctggagca ggaggtgaag ccttccacag agctggagta tctgggccca
     1621 gacgagaacg attaagaccc agctttcttg tacaaagttg gcattataag aaagcattgc
     1681 ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat
     1741 ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc
     1801 tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga
     1861 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa
     1921 cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
     1981 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc
     2041 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
     2101 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
     2161 atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc
     2221 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
     2281 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
     2341 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
     2401 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca
     2461 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
     2521 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
     2581 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     2641 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
     2701 gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg
     2761 acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta cgcgtcgttc
     2821 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2881 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2941 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     3001 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     3061 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     3121 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     3181 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     3241 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     3301 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     3361 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     3421 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     3481 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     3541 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     3601 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     3661 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     3721 agtgagcgca acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa
     3781 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     3841 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     3901 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     3961 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta
     4021 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
//
