LOCUS       sequence-35563-e         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller 
            JA, Somia NV, Bogdanove AJ, Voytas DF
  TITLE     Efficient design and assembly of custom TALEN and other TAL 
            effector-based constructs for DNA targeting.
  JOURNAL   Nucleic Acids Res. 2011 Jul;39(12):e82. doi: 10.1093/nar/gkr218
  PUBMED    21493687
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-07"; volume: "39(12):e82. doi"; pages: " 
            10.1093/nar/gkr21"
COMMENT     Sequence Label: pFUS_B4
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..368)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGAGLDSH
                     HLVRGQAMRRLRLAVVLQ"
     primer_bind     5..21
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     218..240
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     233..249
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     259..278
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        259..277
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(325..345)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(325..343)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(364..380)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(364..380)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(377..399)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    388..404
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(412..418,419..436,437..442))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    457..478
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cgttgtaaaa cgacggccag tcttaagcgt ctcatggcct gaccccggac caagtggtgg
       61 ctatcgagac cggcgccgct acagggcgcg tcccattcgc cattcaggct gcgcaactgt
      121 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      181 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      241 acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac cgggcccccc
      301 ctcgaggtcc tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc
      361 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
      421 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
      481 tgcgttgcgc tcactgcccg ctttccaccg gtggtctcct gttgccggtg ctgtgccagg
      541 accatggcct gaccccggac caagtggtgg ctatcgagac gtctagacca gccaggacag
      601 aaatgcctcg acttcgctgc tacccaaggt tgccgggtga cgcacaccgt ggaaacggat
      661 gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg
      721 tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgca
//
