LOCUS       sequence-355649-        8575 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pnmr-1::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans AVA/E/D 
            premotor IN and others expression of miniSOG-SL2 RFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8575)
  AUTHORS   Gao S, Guan SA, Fouad AD, Meng J, Kawano T, Huang YC, Li Y, Alcaire 
            S, Hung W, Lu Y, Qi YB, Jin Y, Alkema M, Fang-Yen C, Zhen M
  TITLE     Excitatory motor neurons are local oscillators for backward 
            locomotion.
  JOURNAL   Elife. 2018 Jan 23;7. pii: 29915. doi: 10.7554/eLife.29915.
  PUBMED    29360035
REFERENCE   2  (bases 1 to 8575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-01-23"; volume: "7. pii: 29915. doi"; pages: " 
            10.7554/eLife.29915"
COMMENT     Sequence Label: pJH2827
FEATURES             Location/Qualifiers
     source          1..8575
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    89..110
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(125..130,131..148,149..155)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    163..179
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     168..190
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     187..203
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     187..203
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3810..4130
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     primer_bind     complement(6347..6369)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6469..6488
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6566..6584)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6652..6756
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6757..6825,6826..7617)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6975..6994)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7788..8376
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8277..8296
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8530..8547
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
       61 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
      121 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
      181 ttcacacagg aaacagctat gaccatgatt acgccaagct gtaagtttaa acatgatctt
      241 actaactaac tattctcatt taaattttca gagcttaaaa atggctgaaa tcactcacaa
      301 cgatggatac gctaacaact tggaaatgaa ataagcttgg gctgcagcat gcggccgctg
      361 tggtctcact ttgaacgaca aaagaaaacc aaatattgta aaggaatagt acagataaaa
      421 gggaacaagt gataccagct gatggatgta ggtcagcgac aaaagacagg agacgtatag
      481 tttgatgatt atggaaccaa actcagaatt taatgttgga gacgctataa aaattgaatt
      541 gagaaattga aaccatattc ttgaatatct ctcaatttgg aatttggaag tagccgtaaa
      601 atcggctagc tagaacataa aaggactgat ttgcaacctt gaccattcat gatatcattt
      661 caataaaaga agagattcca atgaccaatc ttcaagaatc acaataatca caacaaaact
      721 aagcaaaagt aacaacaaat tgttcaggta gacaagtaga catttggaca cattttgcgg
      781 ggatgtgatg actgtataat aaaaataagt ggacactgag agagagagga ggtcagcagg
      841 aaaaaagaac atcgaactgc ttgacgaatt gaaattagaa tgaggtgtca ggcaggtgac
      901 gattacatgg ttaattatta gtgttctttt tcttcttttt gtggatgccg attagttgaa
      961 ataattgcat ggaaagtgag aattcaatgt gaatctcttt cttattatgt ttcaagtggt
     1021 atctggatgg aagcttgtac ttttatgaat cgaaaattgc gcccaaggtt gattttgaga
     1081 ttattcagta aatttttgtg ctgattattc agttaatgta gaacagagac gcggatgaaa
     1141 acttttttaa tttctaaaaa aaaagacaaa atgtggttaa aaagtttgga aaataattgt
     1201 tatatacttc ttgagagctt attcaaacgg aaaccaatca taaatagtcg aatagtcatt
     1261 tagaaaagag aaactagttg atgtagagag attcaaaaag taaaatatgc aaatgatccg
     1321 tttcttagat ttagtaaaca tctaccaaat gaaacaggaa ttgtcgaaac aatttagaaa
     1381 aggagaagga aacatttgtt tattttcccc tttaaatggc ggtagaggta aaacagcaat
     1441 acacatagta caaggttata ctactttcaa aagaatttga catcccgccg agaaaatgtg
     1501 gtgattatat ggtaaagttt tcagttggat attatcttct acagacggtg attcaaaatg
     1561 atcaaaaggg caagtgggtg gtaggatttg aaaaattgag atatagaacg tgcgattttg
     1621 gtaaaacttt ttgaacacaa ggcatatttt tgaactttga aaaacgcaaa atttaaattt
     1681 tgttaattac atatattttg aacagattat gaatataagc aaggctacat aaaatatgta
     1741 tattttcttt ttacaaatat tcgtacactg ttaaatttat agaaaaatca tttatattca
     1801 gaaaaaccta tttgaacaat ttttagttgc tcaataacca atttgcacaa atttagaaaa
     1861 tataatgggg gcttttaaaa atttaaatcc tcagcgaata ttcctttgcg attttttcga
     1921 acgttttctt tcagattaaa aaatctgaag cagaagtaaa tgaactacca tagaaaattc
     1981 accaaggatc acatttttac aaagcctccg cccaattgta aacttgggaa tttttgcttg
     2041 ttttcttttt tttttcaaaa aaggaatagt tgttttaaag taatttattc ccctatgctt
     2101 tctccagcaa tcagttgttc tccccatact ttacacaatt atcaacaact tcacatagaa
     2161 aacaaaacaa caaatcagaa attcccaccg gtcacagtca cagattcccc atgttttgtt
     2221 catctcattt ctgcaagaaa gtgacatcgc tgccacccaa ttcgacaaat tgtccaagtg
     2281 gtcatcagga agatatgacc ttctttttaa tgataattta gactacaaag ggcgtcaaaa
     2341 aagattcaat gatcaggggt aattgctgta aaatgtagtt tgtatgtgtg tttaccgtag
     2401 cctataactg taaaaattgg ttttttaaaa agtatttctg agattaaaag gctccccaag
     2461 gatgcacgat tctagctaga aatgaacatt ccatgggttg tgaagatgct tttatctcac
     2521 tttagaatgt ctgactccgc ccctctgtgt acgaattgaa gtgctctttt gtgatgatca
     2581 tagctcctcc ttgcggcgac gcccgcacac agcacgagat attacacgga atacttatgt
     2641 gattcatagg aaacaaggaa atcgaaaaaa taataggatt ttgaggaggt atttttagat
     2701 gaatgcttat gatgttatga tgaacccaag aaattctgaa tagacatatg tcagaacggg
     2761 gtaggagctc cagtgtcgct tttgaaataa aaaatcaagt ggtatacttc tcgtatatac
     2821 ccttctgaac taataaaata atttatcttt tgaaaaagtt tgaaaaagaa aaatgcccaa
     2881 aacttgaatt ttaatgtttt gtattttgag catatcgttc aaacgtcgtg atctgaaagt
     2941 agaaagatct aaatattcgt gacacagttt ggtttgaagg tcttagtggt aattttttat
     3001 tacagaagca acaaaaagtt gacatatttt cagaatttcc aaaaaaacgt ttaaaatttc
     3061 aatttcttcg atagagttct aaaaattttt tggaacggat tttgtaatat ccgctgataa
     3121 ccgttcaaga acaatttgaa ttttgaaata atttaatcaa ctagtatcca gtgctctttc
     3181 tcactgctcc acccattttt caacccattt cctttactca atttcgcccg ttttcctttt
     3241 gctcaaatgt gcccaccaaa tcatctttct tcgtcttctt ttctcttatt cggaaggaga
     3301 agaggtgaaa gtagaataag tttttttgtt gaaaagagag gagcacggcc acttttctct
     3361 tatttccatc cttctactac ctcgttttac tttccattta aaaacccgtt taaagttcca
     3421 aaatttcaat tatcttgctt ctgtttggaa cacgacaaac tttagttttg ttacagatac
     3481 ccgggattgg ccaaaggacc caaaggtatg tttcgaatga tactaacata acatagaaca
     3541 ttttcaggag gacccttggc tagcgtcgac ggatcccgat gtcggacaca attcttggtt
     3601 tcaacaaatc aaacgtcgtt ttggctgctg gaattgctgg agccgctttc ctcggctact
     3661 gcatttactt cgatcataag agaatcaacg ctccagacta caaggacaag attaggcaaa
     3721 gtcagtgttt taacaacata tttccttcgg atttttatct aaaaacaact tattttcttt
     3781 cagagagacg tgcccaggct ggggatccta tggagaaaag tttcgtgata actgatccac
     3841 ggctgccaga caatcccatc atcttcgcat ccgatggctt cctggagctg accgagtatt
     3901 ccagagagga gatcctgggc cgcaatggcc gctttctgca gggaccagag acagaccagg
     3961 ccacagtgca gaagattcgc gatgccatta gagatcagcg cgagattacc gtgcagctga
     4021 taaactacac aaaaagcggg aagaaattct ggaacctcct gcacctccag cccatgaggg
     4081 accagaaggg tgagctccag tatttcatcg gagtgcagct ggatggataa agatccccga
     4141 gatcttcagc gctcagcatt cgtagaattg taagtttaaa ctttctcata ctaactaacg
     4201 cgctctattt aaattttcag aattcgctgt ctcatcctac tttcacctag ttaactgctt
     4261 gtcttaaaat ctatgcttct ctttagtatc taaaattttc ctagaagctt acaagtatat
     4321 aaatggtctc ttctcaataa aggttgtata tttattcatc ttattgaatc tgccatttcc
     4381 tcgtttttgc gagtttatat accttccaat tttctttcta ttgtattttc aacttctaat
     4441 tttaattcag ggaaactgct tcaacgcatc ggtaccctat ggtctcaaag ggtgaagaag
     4501 ataacatggc aattattaaa gagtttatgc gtttcaaggt gcatatggag ggatctgtca
     4561 atgggcatga gtttgaaatt gaaggtgaag gagaaggccg accatatgag ggaacacaaa
     4621 ccgcaaaact aaaggtaagt ttaaacatat atatactaac taaccctgat tatttaaatt
     4681 ttcaggtaac taaaggcgga ccattaccat tcgcctggga catcctctct ccacagttca
     4741 tgtatggaag taaagcttat gttaaacatc cggcagatat accagattat ttgaaacttt
     4801 cattcccgga gggttttaag tgggaacgcg taatgaattt tgaagacgga ggagttgtta
     4861 cagtgacgca agactcaagg taagtttaaa cagttcggta ctaactaacc atacatattt
     4921 aaattttcag cctccaagat ggagaattta tttataaagt caaacttcga ggaacgaatt
     4981 tcccctcgga tggatctgtt atgcagaaga agactatggg atgggaagct tcaagtgaaa
     5041 gaatgtaccc tgaagacggt gctcttaagg gagagattaa acaacgtctt aaattgaaag
     5101 atggaggaca ttacgatgct gaggtaagtt taaacatgat tttactaact aactaatctg
     5161 atttaaattt tcaggtgaag acaacttaca aagccaaaaa accagttcag ctgccaggag
     5221 cgtacaatgt taatattaaa ctggatatca cctcccacaa cgaggattac actatcgttg
     5281 agcaatatga aagagctgaa gggcggcact cgacaggtgg catggatgaa ttgtataagt
     5341 aggagctccc gagatcttca gcgctcagca ttcgtagaat tgtaagttta aactttctca
     5401 tactaactaa cgcgctctat ttaaattttc agaattccaa ctgagcgccg gtcgctacca
     5461 ttaccaactt gtctggtgtc aaaaataata ggggccgctg tcatcagagt aagtttaaac
     5521 tgagttctac taactaacga gtaatattta aattttcagc atctcgcgcc cgtgcctctg
     5581 acttctaagt ccaattactc ttcaacatcc ctacatgctc tttctccctg tgctcccacc
     5641 ccctattttt gttattatca aaaaacttct cttaatttct ttgtttttta gcttctttta
     5701 agtcacctct aacaatgaaa ttgtgtagat tcaaaaatag aattaattcg taataaaaag
     5761 tcgaaaaaaa ttgtgctccc tccccccatt aataataatt ctatcccaaa atctacacaa
     5821 tgttctgtgt acacttctta tgttttttac ttctgataaa tttttttgaa acatcataga
     5881 aaaaaccgca cacaaaatac cttatcatat gttacgtttc agtttatgac cgcaattttt
     5941 atttcttcgc acgtctgggc ctctcatgac gtcaaatcat gctcatcgtg aaaaagtttt
     6001 ggagtatttt tggaattttt caatcaagtg aaagtttatg aaattaattt tcctgctttt
     6061 gctttttggg gtttccccta ttgtttgtca agatttcgag gacggcgttt ttcttgctaa
     6121 aatcacaagt attgatgagc acgatgcaag aaagatcgga agaaggtttg ggtttgaggc
     6181 tcagtggaag gtgagtagaa gttgataatt tgaaagtgga gtagtgtcta tggggttttt
     6241 gccttaaatg acagaataca ttcccaatat accaaacata actgtttcct actagtcggc
     6301 cgtacgggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     6361 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     6421 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
     6481 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     6541 aataccgcat caggcggcct taagggcctc gtgatacgcc tatttttata ggttaatgtc
     6601 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     6661 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     6721 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     6781 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     6841 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     6901 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     6961 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     7021 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     7081 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     7141 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     7201 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     7261 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     7321 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     7381 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     7441 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     7501 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     7561 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     7621 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     7681 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     7741 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     7801 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     7861 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     7921 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     7981 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     8041 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     8101 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     8161 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     8221 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     8281 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     8341 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     8401 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     8461 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     8521 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaac
//
