LOCUS       sequence-355652-        4027 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ddGFP sensor of Cdc42.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Kim J , Lee S, Jung K, Oh WC, Kim N, Son S, Jo Y, Kwon HB,  Heo WD
  TITLE     Intensiometric biosensors visualize the activity of multiple small 
            GTPases in vivo.
  JOURNAL   Nat Commun. 2019 Jan 14;10(1):211. doi: 10.1038/s41467-018-08217-3.
  PUBMED    30643148
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-01-14"; volume: "10(1):211. doi"; pages: " 
            10.1038/s41467-018-08217-3"
COMMENT     Sequence Label: pAAV-CAG-G-Cdc42
FEATURES             Location/Qualifiers
     source          1..4027
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     405..427
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     489..508
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1461..1517
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    2864..3452
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2917..2937)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3335..3346)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3495..3616
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3506..3525)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3560..3579
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   3778..3907
                     /label=AAV2 ITR
ORIGIN
        1 gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga
       61 aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg
      121 cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc
      181 cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct
      241 tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt tctgtggctg
      301 cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg ctcggggctg
      361 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
      421 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
      481 gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattgctcga
      541 gcgctagcac catggccacc atcaaagagt tcatgcgctt caaggtgcgc atggagggct
      601 ccatgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
      661 cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc
      721 tgtcccccca gttcatgtac ggctccgagg cgtacgtgag gcaccccgcc gacatccccg
      781 attacaagaa gctgcccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgaag
      841 acggcggtct ggtgaccgtt acccaggact cctccctgca ggacggcacg ctgatctgca
      901 aggtgaagat gcgcggcacc aacttccccc ccgacggccc cgtaatgcag aagaagacca
      961 tgggctggga ggcctccacc gagatgctgt accccgaaga cggcgtgctg aagggccata
     1021 gctatcaggc cctgaagctg aaggacggcg gccactacct ggtggagttc gagaccatct
     1081 acatggccaa gaagcccgtg caactgcccg gcgattactg tgtggacacc aagctggaca
     1141 tcacctccca caacgaggac tacaccatcg tggaacagta cgggcgctcc gagggccgcc
     1201 accgtctggg catggacgag cggtacaagt tagggcatgg tacaggtagc acaggcagcg
     1261 gtagctccgg taccctgtac aagggcagcg gcagctccat tttacctgga gataaaacaa
     1321 ataaaaagaa agagaaagag cggccagaga tttctctccc ttcagatttt gaacacacaa
     1381 ttcatgtcgg ttttgatgct gtcacagggg agtttacggg aatgccagag cagtgggccc
     1441 gcttgcttca gggaagcgga gctactaact tcagcctgct gaagcaggct ggagacgtgg
     1501 aggagaaccc tggaccaatg gtgagcaaga gcgaggaggt catcaaagag ttcatgcgct
     1561 tcaaggtgcg cttggagggc tccatgaacg gccacgagtt cgagatcgag ggcgagggcg
     1621 agggccgccc ttacgagggc acccagaccg ccaagctgaa ggtgaccaag ggcggccccc
     1681 tgcccttcgc ctgggacatc ctgtcccccc tgatcatgta cggctccaag atgtacgtga
     1741 agcaccccgc cgacgtcccc gattacatga agctgtcctt ccccgagggc ttcaagtggg
     1801 agcgcgtgat gcacttcgag gacggcggtc tggtgaccgc gacacaggac tcctccctgc
     1861 aggacggcac gctgatctac aaggtgaaga tgcgcggcac caacttcccc cccgacggcc
     1921 ccgtaatgca gaagaagacc ttgggctggg attatgccac cgagcgcctg taccccgaag
     1981 aaggcgtgct gaagggcgag ctcctggggc gcctgaagct gaaggacggc ggcctcaacc
     2041 tggtggagtc caagaccatc tacatggcca agaagcccgt gcaactgccc ggctactact
     2101 tcgtggacac caagctggac atcacctccc acaacgagga ctacaccatc gtggaacagt
     2161 acgagcgctc cgagggccgc caccacctgg ggcatggtac aggtagcaca ggcagcggta
     2221 gctccggtac cctgtacaag tccggactca gatctcgagc tcaagcttcg aattcggcaa
     2281 tgcagacaat taagtgtgtt gttgtgggcg atggtgctgt tggtaaaaca tgtctcctga
     2341 tatcctacac aacaaacaaa tttccatcgg aatatgtacc gactgttttt gacaactatg
     2401 cagtcacagt tatgattggt ggagaaccat atactcttgg actttttgat actgcagggc
     2461 aagaggatta tgacagatta cgaccgctga gttatccaca aacagatgta tttctagtct
     2521 gtttttcagt ggtctctcca tcttcatttg aaaacgtgaa agaaaagtgg gtgcctgaga
     2581 taactcacca ctgtccaaag actcctttct tgcttgttgg gactcaaatt gatctcagag
     2641 atgacccctc tactattgag aaacttgcca agaacaaaca gaagcctatc actccagaga
     2701 ctgctgaaaa gctggcccgt gacctgaagg ctgtcaagta tgtggagtgt tctgcactta
     2761 cacagagagg tctgaagaat gtgtttgatg aggctatcct agctgccctc gagcctccgg
     2821 aaactcaacc caaaaggaag tgctgtatat tctaaatctc cggaatcaac ctctggatta
     2881 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     2941 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     3001 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     3061 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     3121 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     3181 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     3241 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     3301 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     3361 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     3421 gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacccg ggcggccgct
     3481 tcgagcagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     3541 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     3601 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     3661 gatgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga
     3721 tcttcctaga gcatggctac gtagataagt agcatggcgg gttaatcatt aactacaagg
     3781 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
     3841 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
     3901 cgcgcagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     3961 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     4021 atcagct
//
