LOCUS       sequence-355654-        6314 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plgc-55B::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans AVB 
            premotor IN and other neurals expression of miniSOG-SL2 RFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6314)
  AUTHORS   Gao S, Guan SA, Fouad AD, Meng J, Kawano T, Huang YC, Li Y, Alcaire 
            S, Hung W, Lu Y, Qi YB, Jin Y, Alkema M, Fang-Yen C, Zhen M
  TITLE     Excitatory motor neurons are local oscillators for backward 
            locomotion.
  JOURNAL   Elife. 2018 Jan 23;7. pii: 29915. doi: 10.7554/eLife.29915.
  PUBMED    29360035
REFERENCE   2  (bases 1 to 6314)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-01-23"; volume: "7. pii: 29915. doi"; pages: " 
            10.7554/eLife.29915"
COMMENT     Sequence Label: pJH2890
FEATURES             Location/Qualifiers
     source          1..6314
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     128..147
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     381..398
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    515..536
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(551..556,557..574,575..581)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    589..605
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     594..616
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     613..629
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     613..629
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1975..2295
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     primer_bind     complement(4512..4534)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4634..4653
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4731..4749)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4817..4921
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4922..4990,4991..5782)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5140..5159)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(5953..6314,1..227)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
       61 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
      121 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
      181 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
      241 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
      301 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
      361 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
      421 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
      481 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
      541 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
      601 aacaatttca cacaggaaac agctatgacc atgattacgc caagctgtaa gtttaaacat
      661 gatcttacta actaactatt ctcatttaaa ttttcagagc ttaaaaatgg ctgaaatcac
      721 tcacaacgat ggatacgcta acaacttgga aatgaaataa gcttgggctg cagcatgcgg
      781 ccgctgttgc ttttcttctc ctcacccccc gtcgtctctt cttttttact attccccacg
      841 agaaactgcc caccacaaaa acttactttc cagaaaaata ttggcaaata gatgtcgaaa
      901 tgaaatggct agctttgatg atggtaagcg tttttttttt tctacaaaat tgtggattaa
      961 gaatcttcca aaaccgttat actacactat atccaaatat aaaaaaattt tggcccaggc
     1021 ggggggtcga accagggcct tcagattggt agaccacccc gttaccgcct gagccacttt
     1081 ttcccatttt tgaagttgat ggctaagatt ggagtttgaa cgattgaacg attttagagc
     1141 aaaattttat ggggattctg aaaatatagt ttattttaag tttggagcat tttttatggg
     1201 ctcaaaatga ctcggaagct cgaaaatctg aaattttcaa aacttttaat gtgggctttt
     1261 caaatttcaa atttccgtga aaaatattga atattggttg taaattttaa gaaatatatg
     1321 aatttcaaca ctttattata cgaaaaactg catgcaaaaa ttttcaggtc aaatcatgtg
     1381 caaattttgt gctgatttcg aatttttata tcattttgta ccaaaacccc tagaatttta
     1441 gaaacttttg aacttctgtt aagtaatgac tcacttttcg agcaaaatcg aatgttccaa
     1501 aaataggcaa aacatctgcc cgcgcattaa gctcaaccac gacaatttta ttggacagga
     1561 agttgtttgg tgactaattg aatttcaatt ccccgtcccc cctctccaca caaataccac
     1621 tacgaacatc tcttttgacg gatccccggg attggccaaa ggacccaaag gtatgtttcg
     1681 aatgatacta acataacata gaacattttc aggaggaccc ttggctagcg tcgacggatc
     1741 ccgatgtcgg acacaattct tggtttcaac aaatcaaacg tcgttttggc tgctggaatt
     1801 gctggagccg ctttcctcgg ctactgcatt tacttcgatc ataagagaat caacgctcca
     1861 gactacaagg acaagattag gcaaagtcag tgttttaaca acatatttcc ttcggatttt
     1921 tatctaaaaa caacttattt tctttcagag agacgtgccc aggctgggga tcctatggag
     1981 aaaagtttcg tgataactga tccacggctg ccagacaatc ccatcatctt cgcatccgat
     2041 ggcttcctgg agctgaccga gtattccaga gaggagatcc tgggccgcaa tggccgcttt
     2101 ctgcagggac cagagacaga ccaggccaca gtgcagaaga ttcgcgatgc cattagagat
     2161 cagcgcgaga ttaccgtgca gctgataaac tacacaaaaa gcgggaagaa attctggaac
     2221 ctcctgcacc tccagcccat gagggaccag aagggtgagc tccagtattt catcggagtg
     2281 cagctggatg gataaagatc cccgagatct tcagcgctca gcattcgtag aattgtaagt
     2341 ttaaactttc tcatactaac taacgcgctc tatttaaatt ttcagaattc gctgtctcat
     2401 cctactttca cctagttaac tgcttgtctt aaaatctatg cttctcttta gtatctaaaa
     2461 ttttcctaga agcttacaag tatataaatg gtctcttctc aataaaggtt gtatatttat
     2521 tcatcttatt gaatctgcca tttcctcgtt tttgcgagtt tatatacctt ccaattttct
     2581 ttctattgta ttttcaactt ctaattttaa ttcagggaaa ctgcttcaac gcatcggtac
     2641 cctatggtct caaagggtga agaagataac atggcaatta ttaaagagtt tatgcgtttc
     2701 aaggtgcata tggagggatc tgtcaatggg catgagtttg aaattgaagg tgaaggagaa
     2761 ggccgaccat atgagggaac acaaaccgca aaactaaagg taagtttaaa catatatata
     2821 ctaactaacc ctgattattt aaattttcag gtaactaaag gcggaccatt accattcgcc
     2881 tgggacatcc tctctccaca gttcatgtat ggaagtaaag cttatgttaa acatccggca
     2941 gatataccag attatttgaa actttcattc ccggagggtt ttaagtggga acgcgtaatg
     3001 aattttgaag acggaggagt tgttacagtg acgcaagact caaggtaagt ttaaacagtt
     3061 cggtactaac taaccataca tatttaaatt ttcagcctcc aagatggaga atttatttat
     3121 aaagtcaaac ttcgaggaac gaatttcccc tcggatggat ctgttatgca gaagaagact
     3181 atgggatggg aagcttcaag tgaaagaatg taccctgaag acggtgctct taagggagag
     3241 attaaacaac gtcttaaatt gaaagatgga ggacattacg atgctgaggt aagtttaaac
     3301 atgattttac taactaacta atctgattta aattttcagg tgaagacaac ttacaaagcc
     3361 aaaaaaccag ttcagctgcc aggagcgtac aatgttaata ttaaactgga tatcacctcc
     3421 cacaacgagg attacactat cgttgagcaa tatgaaagag ctgaagggcg gcactcgaca
     3481 ggtggcatgg atgaattgta taagtaggag ctcccgagat cttcagcgct cagcattcgt
     3541 agaattgtaa gtttaaactt tctcatacta actaacgcgc tctatttaaa ttttcagaat
     3601 tccaactgag cgccggtcgc taccattacc aacttgtctg gtgtcaaaaa taataggggc
     3661 cgctgtcatc agagtaagtt taaactgagt tctactaact aacgagtaat atttaaattt
     3721 tcagcatctc gcgcccgtgc ctctgacttc taagtccaat tactcttcaa catccctaca
     3781 tgctctttct ccctgtgctc ccacccccta tttttgttat tatcaaaaaa cttctcttaa
     3841 tttctttgtt ttttagcttc ttttaagtca cctctaacaa tgaaattgtg tagattcaaa
     3901 aatagaatta attcgtaata aaaagtcgaa aaaaattgtg ctccctcccc ccattaataa
     3961 taattctatc ccaaaatcta cacaatgttc tgtgtacact tcttatgttt tttacttctg
     4021 ataaattttt ttgaaacatc atagaaaaaa ccgcacacaa aataccttat catatgttac
     4081 gtttcagttt atgaccgcaa tttttatttc ttcgcacgtc tgggcctctc atgacgtcaa
     4141 atcatgctca tcgtgaaaaa gttttggagt atttttggaa tttttcaatc aagtgaaagt
     4201 ttatgaaatt aattttcctg cttttgcttt ttggggtttc ccctattgtt tgtcaagatt
     4261 tcgaggacgg cgtttttctt gctaaaatca caagtattga tgagcacgat gcaagaaaga
     4321 tcggaagaag gtttgggttt gaggctcagt ggaaggtgag tagaagttga taatttgaaa
     4381 gtggagtagt gtctatgggg tttttgcctt aaatgacaga atacattccc aatataccaa
     4441 acataactgt ttcctactag tcggccgtac gggccctttc gtctcgcgcg tttcggtgat
     4501 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     4561 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     4621 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
     4681 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc ggccttaagg gcctcgtgat
     4741 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     4801 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     4861 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     4921 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     4981 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     5041 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     5101 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     5161 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     5221 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     5281 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     5341 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     5401 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     5461 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     5521 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     5581 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     5641 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     5701 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     5761 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     5821 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     5881 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     5941 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     6001 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     6061 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     6121 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     6181 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     6241 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     6301 ggagcgaacg acct
//
