LOCUS       sequence-355658-        8350 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pceh-12::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans VB-MN 
            -specific expression of miniSOG-SL2 RFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8350)
  AUTHORS   Gao S, Guan SA, Fouad AD, Meng J, Kawano T, Huang YC, Li Y, Alcaire 
            S, Hung W, Lu Y, Qi YB, Jin Y, Alkema M, Fang-Yen C, Zhen M
  TITLE     Excitatory motor neurons are local oscillators for backward 
            locomotion.
  JOURNAL   Elife. 2018 Jan 23;7. pii: 29915. doi: 10.7554/eLife.29915.
  PUBMED    29360035
REFERENCE   2  (bases 1 to 8350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-01-23"; volume: "7. pii: 29915. doi"; pages: " 
            10.7554/eLife.29915"
COMMENT     Sequence Label: pJH3409
FEATURES             Location/Qualifiers
     source          1..8350
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     99..118
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     352..369
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    486..507
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(522..527,528..545,546..552)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    560..576
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     565..587
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     584..600
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     584..600
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3982..4302
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     primer_bind     complement(6519..6541)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6641..6660
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6738..6756)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6824..6928
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6929..6997,6998..7789)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7147..7166)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(7960..8350,1..198)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
       61 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
      121 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
      181 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
      241 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
      301 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
      361 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
      421 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
      481 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
      541 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
      601 catgattacg ccaagctgta agtttaaaca tgatcttact aactaactat tctcatttaa
      661 attttcagag cttaaaaatg gctgaaatca ctcacaacga tggatacgct aacaacttgg
      721 aaatgaaata agcttgggct gcagcatgcg gccgctttag cgtatagtat tcggatagaa
      781 atgtgtaaaa atgatgaaaa gcgtagcatt caatgagacc catatggaaa ttatgaaacc
      841 agaataattt ggtaattgta ttagtttaga aatatttgtt taagttattg tgttcaattt
      901 gttttgttta ggcagcaact gatttggatg agaagagtta aaaataggtt atttaatcaa
      961 taaaagcaat atgaaaagta ttcctcagta gggtctaaca ataaatacac taatggtcaa
     1021 aataatataa cttattccag tctaaaaata ttcacgaatt ttcttctcct aaatactaga
     1081 aattcttaaa tttatcaaaa accagaaaaa attgcaacat ctacagtcag tttaaattgt
     1141 ttttgaattt tggtattttg gtatttgaga aacgttcaaa tcagatttta aaaatattgg
     1201 ataggtattt tcgcactcat acatctttga aataaaacat taagtttttt ctcaatacgt
     1261 ttgaaaaaag tacagtacta catagtatat gcacacattt tccaaatata tattatcaac
     1321 agaaaactat tctcatcatt ctattttccc aatattccca tccactttga atcactaaca
     1381 acaatcatgt gatttcctaa atgttttcct tttttcaaat tgaagaaaat caacgctgcg
     1441 gaacacattt tcccgaaaaa agccgtccga gttgttcccg tccacacctt ttttccctct
     1501 caagagttat tcttttgtag attctttgga tcattcactt gatagcctaa tagtgggcgt
     1561 gtgtttgtct gtgctccgtt acgtgaaaaa cggaacaaat attttttgaa aaaaaaacat
     1621 tcgaattttt ccgtcgcgtc tcgaaatgcc catttctcct gctggtaggt ttttatagac
     1681 ctctatttga aaatttagtt atgaatgttt aaataagtgt ctctaaaact ataaaaaacc
     1741 aatttttaga ggaatcttgg tagatgctca cttttcagct cacattgatt aaatcctcaa
     1801 aagttcacat ataatcattg aaattgtttt ttgaaataat tttttaaaaa gttatgtttg
     1861 actcattctg ttttggttac tttggcaact acctgaacac agtctcctgt atcaattgct
     1921 cttaaacttt ttaatcgaaa ttttcagatt caacaagcat tgctcgtcaa aaacaacgtc
     1981 gcatcgaacc acttgtaatg cttgattctc gggattacgg tagttggagg aatggaacaa
     2041 ctccatcgcc gagcacaaat aacaaaagcc ctccggtatt tcaaaatgag aagaagagag
     2101 ttcattggcg gaaataattc ttgtttcaat ttttttttgt attctctgta aactgtgtac
     2161 atattttgta aattggatta ctaatttaaa aataaatact tgtaaaataa aatgaaataa
     2221 ataaataact gtaaaagcgt acttcaggta gatccaaaat atttggagtg gatttaattt
     2281 ttgggttcag gtcaagtctg tgcggcaaac ggcacaaggt tcaagggaca agcaaccgaa
     2341 atcattgata ctaacgaaaa gttgaaatgc agtaagaaga tcaaggaatt accggacaaa
     2401 attattgcca aggaacaaag gcatacgtct cagcttcatt tgcatgatcc tgttcaagta
     2461 ttccgtgaca tcatcaacca ccatcacgtg tgtacctcat accagggctg tgcggcatcc
     2521 tgaattttcg gcgaacgttc gccgacacat aaatttctca taatttttcg gcgatcggca
     2581 tttatcggca cttttcggca tccttaaaaa aaaaattttg tgtttttttt tcaattttct
     2641 aaatctaaat agttgaattc caaagtttta taaagagtgt ctggtttgaa ttttaaagca
     2701 tagatcaatt tcatatttaa acgaccagac tgttgggata gtcaaaaaag gatcccagag
     2761 gcaagaatga ttcaaaataa ttgtgtcgac taaaagaata tggctaataa ttcaaatatg
     2821 ttaatttttg ggaagtgtta gaaatacagg tgatttggga gtgttatgat acaagagaca
     2881 cagagatgtc tttggttgtg ttttatgact ataatgtttg tagatttatg tttcaacgga
     2941 tgaatgtgaa aaacttgaat gtatattcga ttaaattttt aaaaaataaa tttttcacaa
     3001 atgccgaaaa ataccgaaaa aagtcgaaca aaagtcgacc gtttgccgca catctctcat
     3061 acatatgaac gtcatatttg aatattctcg tttaagctga tgtgcacggc atttggaata
     3121 ttacgggaat tgccaaaacc atagaaaata ttttcctaac agcaatcgaa actatttgat
     3181 tccataactc caattccctt ctttaaacta gaaatttata agcacatgcc tgttctaagt
     3241 atccatcaat caaatgccaa gaaaccgcaa aacaaggcga aaattatcag aattctcaac
     3301 aagtcatccg ggcggctccc aagaggcagt gccgcggcgg caacacgctc atcttaaatg
     3361 aaaacccaag aaagagacgc tatataatgt tttccgaaat atcaatcatg cttctcctca
     3421 cacatcagct tctcgtcttc tcgtcctacc gtagtcgctt atgaaccgag ccgcctccct
     3481 agagactatg ggatcctcag cggtaagtga tgggctccgc cttgtaaaat gcaacatttt
     3541 ccctcattca atatgagccg gatgattttt cgcatattta tcatttttgt ttcaaagact
     3601 cctacagtac tcttctaatc gtaaaccttt tcaattgaca ataatttcag acccgggatt
     3661 ggccaaagga cccaaaggta tgtttcgaat gatactaaca taacatagaa cattttcagg
     3721 aggacccttg gctagcgtcg acggatcccg atgtcggaca caattcttgg tttcaacaaa
     3781 tcaaacgtcg ttttggctgc tggaattgct ggagccgctt tcctcggcta ctgcatttac
     3841 ttcgatcata agagaatcaa cgctccagac tacaaggaca agattaggca aagtcagtgt
     3901 tttaacaaca tatttccttc ggatttttat ctaaaaacaa cttattttct ttcagagaga
     3961 cgtgcccagg ctggggatcc tatggagaaa agtttcgtga taactgatcc acggctgcca
     4021 gacaatccca tcatcttcgc atccgatggc ttcctggagc tgaccgagta ttccagagag
     4081 gagatcctgg gccgcaatgg ccgctttctg cagggaccag agacagacca ggccacagtg
     4141 cagaagattc gcgatgccat tagagatcag cgcgagatta ccgtgcagct gataaactac
     4201 acaaaaagcg ggaagaaatt ctggaacctc ctgcacctcc agcccatgag ggaccagaag
     4261 ggtgagctcc agtatttcat cggagtgcag ctggatggat aaagatcccc gagatcttca
     4321 gcgctcagca ttcgtagaat tgtaagttta aactttctca tactaactaa cgcgctctat
     4381 ttaaattttc agaattcgct gtctcatcct actttcacct agttaactgc ttgtcttaaa
     4441 atctatgctt ctctttagta tctaaaattt tcctagaagc ttacaagtat ataaatggtc
     4501 tcttctcaat aaaggttgta tatttattca tcttattgaa tctgccattt cctcgttttt
     4561 gcgagtttat ataccttcca attttctttc tattgtattt tcaacttcta attttaattc
     4621 agggaaactg cttcaacgca tcggtaccct atggtctcaa agggtgaaga agataacatg
     4681 gcaattatta aagagtttat gcgtttcaag gtgcatatgg agggatctgt caatgggcat
     4741 gagtttgaaa ttgaaggtga aggagaaggc cgaccatatg agggaacaca aaccgcaaaa
     4801 ctaaaggtaa gtttaaacat atatatacta actaaccctg attatttaaa ttttcaggta
     4861 actaaaggcg gaccattacc attcgcctgg gacatcctct ctccacagtt catgtatgga
     4921 agtaaagctt atgttaaaca tccggcagat ataccagatt atttgaaact ttcattcccg
     4981 gagggtttta agtgggaacg cgtaatgaat tttgaagacg gaggagttgt tacagtgacg
     5041 caagactcaa ggtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc
     5101 agcctccaag atggagaatt tatttataaa gtcaaacttc gaggaacgaa tttcccctcg
     5161 gatggatctg ttatgcagaa gaagactatg ggatgggaag cttcaagtga aagaatgtac
     5221 cctgaagacg gtgctcttaa gggagagatt aaacaacgtc ttaaattgaa agatggagga
     5281 cattacgatg ctgaggtaag tttaaacatg attttactaa ctaactaatc tgatttaaat
     5341 tttcaggtga agacaactta caaagccaaa aaaccagttc agctgccagg agcgtacaat
     5401 gttaatatta aactggatat cacctcccac aacgaggatt acactatcgt tgagcaatat
     5461 gaaagagctg aagggcggca ctcgacaggt ggcatggatg aattgtataa gtaggagctc
     5521 ccgagatctt cagcgctcag cattcgtaga attgtaagtt taaactttct catactaact
     5581 aacgcgctct atttaaattt tcagaattcc aactgagcgc cggtcgctac cattaccaac
     5641 ttgtctggtg tcaaaaataa taggggccgc tgtcatcaga gtaagtttaa actgagttct
     5701 actaactaac gagtaatatt taaattttca gcatctcgcg cccgtgcctc tgacttctaa
     5761 gtccaattac tcttcaacat ccctacatgc tctttctccc tgtgctccca ccccctattt
     5821 ttgttattat caaaaaactt ctcttaattt ctttgttttt tagcttcttt taagtcacct
     5881 ctaacaatga aattgtgtag attcaaaaat agaattaatt cgtaataaaa agtcgaaaaa
     5941 aattgtgctc cctcccccca ttaataataa ttctatccca aaatctacac aatgttctgt
     6001 gtacacttct tatgtttttt acttctgata aatttttttg aaacatcata gaaaaaaccg
     6061 cacacaaaat accttatcat atgttacgtt tcagtttatg accgcaattt ttatttcttc
     6121 gcacgtctgg gcctctcatg acgtcaaatc atgctcatcg tgaaaaagtt ttggagtatt
     6181 tttggaattt ttcaatcaag tgaaagttta tgaaattaat tttcctgctt ttgctttttg
     6241 gggtttcccc tattgtttgt caagatttcg aggacggcgt ttttcttgct aaaatcacaa
     6301 gtattgatga gcacgatgca agaaagatcg gaagaaggtt tgggtttgag gctcagtgga
     6361 aggtgagtag aagttgataa tttgaaagtg gagtagtgtc tatggggttt ttgccttaaa
     6421 tgacagaata cattcccaat ataccaaaca taactgtttc ctactagtcg gccgtacggg
     6481 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     6541 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     6601 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     6661 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     6721 atcaggcggc cttaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     6781 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     6841 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     6901 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     6961 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     7021 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     7081 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     7141 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     7201 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     7261 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     7321 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     7381 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     7441 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     7501 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     7561 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     7621 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     7681 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     7741 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     7801 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     7861 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     7921 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     7981 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     8041 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     8101 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     8161 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     8221 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     8281 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     8341 acagcgtgag
//
