LOCUS       sequence-355660-        8101 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Punc-129(DB)::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans B-MN 
            -specific expression of miniSOG-SL2 RFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8101)
  AUTHORS   Gao S, Guan SA, Fouad AD, Meng J, Kawano T, Huang YC, Li Y, Alcaire 
            S, Hung W, Lu Y, Qi YB, Jin Y, Alkema M, Fang-Yen C, Zhen M
  TITLE     Excitatory motor neurons are local oscillators for backward 
            locomotion.
  JOURNAL   Elife. 2018 Jan 23;7. pii: 29915. doi: 10.7554/eLife.29915.
  PUBMED    29360035
REFERENCE   2  (bases 1 to 8101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-01-23"; volume: "7. pii: 29915. doi"; pages: " 
            10.7554/eLife.29915"
COMMENT     Sequence Label: pJH3410
FEATURES             Location/Qualifiers
     source          1..8101
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    95..116
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(131..136,137..154,155..161)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    169..185
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     174..196
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     193..209
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     193..209
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3342..3662
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     primer_bind     complement(5879..5901)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6001..6020
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6098..6116)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6184..6288
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6289..6357,6358..7149)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6507..6526)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7320..7908
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7809..7828
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8062..8079
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
       61 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
      121 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
      181 aacaatttca cacaggaaac agctatgacc atgattacgc caagctgtaa gtttaaacat
      241 gatcttacta actaactatt ctcatttaaa ttttcagagc ttaaaaatgg ctgaaatcac
      301 tcacaacgat ggatacgcta acaacttgga aatgaaataa gcttgggctg cagcatgcgg
      361 ccgccgggaa acatgatatc gacggacata tttttgattg attatcataa ttttcgtggg
      421 aatcttcgga acatgctctt tgcggtttat aagtggagat gcgccaacgg gaaacttgtt
      481 ttaaaactat tcctatggtg ctaacatcac cacatgaaaa cttttcaaaa cttttttggg
      541 ttttttattt ttttattatt attcgtatct aaacattgta ggcgttgtga tgttgcccta
      601 aatttcttta aaaagagcga acattttcaa aaatttgcta tttaaatcga atgaaaattt
      661 ccattcaaat atttgaaaaa aattatgaaa aaattccttt tgttgcatct tcatcttcta
      721 tgaaagttac aattttagtt ggatcagaaa tatgagatat caaaaaaaaa ttaagatttt
      781 tttgtttgtt tttttgaagc ctaaaatatt ttgatatcat ttatctctta tgtcttttgt
      841 tttatcgaaa cgtttacaac acatcaacta tgcgtcaata aattttttag gtagttaaac
      901 aaatgttgaa aaaaaaagca aaagcaacca agagctgtta aaatagagaa aatgcagtta
      961 ttttttatat tcaagaaaag ttatcacaga agttccgtaa tgagtttttt ccttatcaac
     1021 atgttcccat caacttctta gtttctcctc attcccattg ttcaacataa ttatcacctc
     1081 tgaccttatt ctgctctttt taagcacagg tgttaatatg ccgtccatca tatttgccct
     1141 ccgtctgccg ctttccttat ctgaccttta catcgccttt ttgtatatgt acataataat
     1201 tgtatcatca tcaacctccg ccataaacat gcatgtcttt ttacctcttt tggcatgtac
     1261 cgttcttctt tgtattttta ttatgtcttc cttccatctt ccctcttttt tacggatttt
     1321 cggattgcgg aagaagaaga tggtcgataa ttaccgtaat gaggtaattt gaaacatatt
     1381 ggacagaaga aatacgcgcg gatgctttcg gtggcaaggt taattcgttg tctttgtttc
     1441 tggaatgtcc ttcataaaaa acacggatta ccacctgttt ttaacgtgga ttaagatttt
     1501 tggaaacaat ttactctgcc taattttttt gtacatttaa aaatgtagac ttaccgaatt
     1561 caccgagctt gaccagtttt aaaggttacc gttcacacta aaaattgttg aaaaaatttt
     1621 cgactaaacc taatgatttt attttaaaaa ataaatgaaa tagcatacaa atttggattt
     1681 ttttcaattt ttttcaagaa aatcatgaaa catttagaaa actcgagtaa caagctttcc
     1741 gttcattctg taatttttaa gttccaaatt taaaaaaatg cattttgatt tactgggatt
     1801 gccgtgctcg taattatcag caaaacattt gccgaattag ccgagctcga caatctattt
     1861 tttcgcaaac ttttgccatg tttcgtaaaa caattttcca ttttatggta gacatttttc
     1921 atcaatgaat tcacaaaatt taaaatcccc actgaaagta ccaaaatctt cctgaaaaat
     1981 ccggcaaaaa atccatctca ttatcatgtc acatatacat gttctattgg cactatgttt
     2041 gggatgtcct ccaattaata cttctgtgtc aatctcatcg gcgcccggcg gccactacca
     2101 aatgcaaaac tttgtttgta aaagaagaag ccaataattt tctcgttatt catttttatt
     2161 actctttttg cgtttcattc ttctttgttc gaattgatga acgcacgttg cttctctctg
     2221 gttttcctgc cgttccttat attgccaagg aaaccaatgc ggattttcgg aagattttct
     2281 tcatgtcctt tagaaaatat caccactttt cttctttttc catttcaaat ttcaatttcc
     2341 atacaactca tcttctatta tagcttttca ggtgttcgac tgtttgaaga taagaatcaa
     2401 ttaaaatttg gttcactgag catgtcatcc acttttcctt ttctttgttc taaaattcac
     2461 caaactgaat tttccttaac cggaaattta catgaaaata agaaacatgg attacatcct
     2521 ttacatatca tttttgtttt tttatgtgta ttccatgttc ccaaaagttt gatgcaattt
     2581 ttgattcttt ttcaattttc caaattcgtg tctcgcaggg aacgattcac agtccaacat
     2641 tcccaaattg cgaaatttta gaatatatat tcccaagcgt cgaatatctc aaaaatttgt
     2701 cgttttctca gtttgacatg tcacctttgc cggttcctct attcgtctat tagctctcca
     2761 gagagcaatg tcatgtcaca cgaagaattc agggacatgt ttcgtttttt tttgtttgca
     2821 tgtggtcacc gatatgggaa cttgaacttt ccaatttttg cttgcttttt cttttccgga
     2881 aacttaatac tatttgagaa actccgcatc ttatttgttt gatcccaaat tctgaaattc
     2941 ctctttgaat ttgatctcaa gcttcactat aaaaaattgt ttcaggaaaa ttggaagagc
     3001 aagcaagaaa acccgggatt ggccaaagga cccaaaggta tgtttcgaat gatactaaca
     3061 taacatagaa cattttcagg aggacccttg gctagcgtcg acggatcccg atgtcggaca
     3121 caattcttgg tttcaacaaa tcaaacgtcg ttttggctgc tggaattgct ggagccgctt
     3181 tcctcggcta ctgcatttac ttcgatcata agagaatcaa cgctccagac tacaaggaca
     3241 agattaggca aagtcagtgt tttaacaaca tatttccttc ggatttttat ctaaaaacaa
     3301 cttattttct ttcagagaga cgtgcccagg ctggggatcc tatggagaaa agtttcgtga
     3361 taactgatcc acggctgcca gacaatccca tcatcttcgc atccgatggc ttcctggagc
     3421 tgaccgagta ttccagagag gagatcctgg gccgcaatgg ccgctttctg cagggaccag
     3481 agacagacca ggccacagtg cagaagattc gcgatgccat tagagatcag cgcgagatta
     3541 ccgtgcagct gataaactac acaaaaagcg ggaagaaatt ctggaacctc ctgcacctcc
     3601 agcccatgag ggaccagaag ggtgagctcc agtatttcat cggagtgcag ctggatggat
     3661 aaagatcccc gagatcttca gcgctcagca ttcgtagaat tgtaagttta aactttctca
     3721 tactaactaa cgcgctctat ttaaattttc agaattcgct gtctcatcct actttcacct
     3781 agttaactgc ttgtcttaaa atctatgctt ctctttagta tctaaaattt tcctagaagc
     3841 ttacaagtat ataaatggtc tcttctcaat aaaggttgta tatttattca tcttattgaa
     3901 tctgccattt cctcgttttt gcgagtttat ataccttcca attttctttc tattgtattt
     3961 tcaacttcta attttaattc agggaaactg cttcaacgca tcggtaccct atggtctcaa
     4021 agggtgaaga agataacatg gcaattatta aagagtttat gcgtttcaag gtgcatatgg
     4081 agggatctgt caatgggcat gagtttgaaa ttgaaggtga aggagaaggc cgaccatatg
     4141 agggaacaca aaccgcaaaa ctaaaggtaa gtttaaacat atatatacta actaaccctg
     4201 attatttaaa ttttcaggta actaaaggcg gaccattacc attcgcctgg gacatcctct
     4261 ctccacagtt catgtatgga agtaaagctt atgttaaaca tccggcagat ataccagatt
     4321 atttgaaact ttcattcccg gagggtttta agtgggaacg cgtaatgaat tttgaagacg
     4381 gaggagttgt tacagtgacg caagactcaa ggtaagttta aacagttcgg tactaactaa
     4441 ccatacatat ttaaattttc agcctccaag atggagaatt tatttataaa gtcaaacttc
     4501 gaggaacgaa tttcccctcg gatggatctg ttatgcagaa gaagactatg ggatgggaag
     4561 cttcaagtga aagaatgtac cctgaagacg gtgctcttaa gggagagatt aaacaacgtc
     4621 ttaaattgaa agatggagga cattacgatg ctgaggtaag tttaaacatg attttactaa
     4681 ctaactaatc tgatttaaat tttcaggtga agacaactta caaagccaaa aaaccagttc
     4741 agctgccagg agcgtacaat gttaatatta aactggatat cacctcccac aacgaggatt
     4801 acactatcgt tgagcaatat gaaagagctg aagggcggca ctcgacaggt ggcatggatg
     4861 aattgtataa gtaggagctc ccgagatctt cagcgctcag cattcgtaga attgtaagtt
     4921 taaactttct catactaact aacgcgctct atttaaattt tcagaattcc aactgagcgc
     4981 cggtcgctac cattaccaac ttgtctggtg tcaaaaataa taggggccgc tgtcatcaga
     5041 gtaagtttaa actgagttct actaactaac gagtaatatt taaattttca gcatctcgcg
     5101 cccgtgcctc tgacttctaa gtccaattac tcttcaacat ccctacatgc tctttctccc
     5161 tgtgctccca ccccctattt ttgttattat caaaaaactt ctcttaattt ctttgttttt
     5221 tagcttcttt taagtcacct ctaacaatga aattgtgtag attcaaaaat agaattaatt
     5281 cgtaataaaa agtcgaaaaa aattgtgctc cctcccccca ttaataataa ttctatccca
     5341 aaatctacac aatgttctgt gtacacttct tatgtttttt acttctgata aatttttttg
     5401 aaacatcata gaaaaaaccg cacacaaaat accttatcat atgttacgtt tcagtttatg
     5461 accgcaattt ttatttcttc gcacgtctgg gcctctcatg acgtcaaatc atgctcatcg
     5521 tgaaaaagtt ttggagtatt tttggaattt ttcaatcaag tgaaagttta tgaaattaat
     5581 tttcctgctt ttgctttttg gggtttcccc tattgtttgt caagatttcg aggacggcgt
     5641 ttttcttgct aaaatcacaa gtattgatga gcacgatgca agaaagatcg gaagaaggtt
     5701 tgggtttgag gctcagtgga aggtgagtag aagttgataa tttgaaagtg gagtagtgtc
     5761 tatggggttt ttgccttaaa tgacagaata cattcccaat ataccaaaca taactgtttc
     5821 ctactagtcg gccgtacggg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
     5881 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     5941 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
     6001 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     6061 gcgtaaggag aaaataccgc atcaggcggc cttaagggcc tcgtgatacg cctattttta
     6121 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     6181 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     6241 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     6301 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     6361 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     6421 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     6481 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     6541 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     6601 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     6661 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     6721 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     6781 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     6841 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     6901 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     6961 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     7021 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     7081 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     7141 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     7201 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     7261 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     7321 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     7381 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     7441 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     7501 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     7561 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     7621 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     7681 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     7741 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     7801 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     7861 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     7921 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     7981 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     8041 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     8101 c
//
